Job ID = 14168232 SRX = SRX7175257 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 18364782 spots for SRR10485697/SRR10485697.sra Written 18364782 spots for SRR10485697/SRR10485697.sra fastq に変換しました。 bowtie でマッピング中... Your job 14169410 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:01:21 18364782 reads; of these: 18364782 (100.00%) were paired; of these: 1075908 (5.86%) aligned concordantly 0 times 12667351 (68.98%) aligned concordantly exactly 1 time 4621523 (25.17%) aligned concordantly >1 times ---- 1075908 pairs aligned concordantly 0 times; of these: 218567 (20.31%) aligned discordantly 1 time ---- 857341 pairs aligned 0 times concordantly or discordantly; of these: 1714682 mates make up the pairs; of these: 1100528 (64.18%) aligned 0 times 356586 (20.80%) aligned exactly 1 time 257568 (15.02%) aligned >1 times 97.00% overall alignment rate Time searching: 01:01:21 Overall time: 01:01:21 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 432768 / 17466617 = 0.0248 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 17:37:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7175257/SRX7175257.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7175257/SRX7175257.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7175257/SRX7175257.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7175257/SRX7175257.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 17:37:33: #1 read tag files... INFO @ Fri, 10 Dec 2021 17:37:33: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 17:37:43: 1000000 INFO @ Fri, 10 Dec 2021 17:37:52: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 17:38:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7175257/SRX7175257.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7175257/SRX7175257.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7175257/SRX7175257.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7175257/SRX7175257.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 17:38:01: #1 read tag files... INFO @ Fri, 10 Dec 2021 17:38:01: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 17:38:02: 3000000 INFO @ Fri, 10 Dec 2021 17:38:12: 4000000 INFO @ Fri, 10 Dec 2021 17:38:12: 1000000 INFO @ Fri, 10 Dec 2021 17:38:21: 5000000 INFO @ Fri, 10 Dec 2021 17:38:24: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 17:38:31: 6000000 INFO @ Fri, 10 Dec 2021 17:38:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7175257/SRX7175257.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7175257/SRX7175257.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7175257/SRX7175257.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7175257/SRX7175257.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 17:38:31: #1 read tag files... INFO @ Fri, 10 Dec 2021 17:38:31: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 17:38:34: 3000000 INFO @ Fri, 10 Dec 2021 17:38:42: 7000000 INFO @ Fri, 10 Dec 2021 17:38:42: 1000000 INFO @ Fri, 10 Dec 2021 17:38:46: 4000000 INFO @ Fri, 10 Dec 2021 17:38:53: 8000000 INFO @ Fri, 10 Dec 2021 17:38:53: 2000000 INFO @ Fri, 10 Dec 2021 17:38:58: 5000000 INFO @ Fri, 10 Dec 2021 17:39:03: 3000000 INFO @ Fri, 10 Dec 2021 17:39:03: 9000000 INFO @ Fri, 10 Dec 2021 17:39:09: 6000000 INFO @ Fri, 10 Dec 2021 17:39:13: 4000000 INFO @ Fri, 10 Dec 2021 17:39:13: 10000000 INFO @ Fri, 10 Dec 2021 17:39:20: 7000000 INFO @ Fri, 10 Dec 2021 17:39:24: 5000000 INFO @ Fri, 10 Dec 2021 17:39:24: 11000000 INFO @ Fri, 10 Dec 2021 17:39:31: 8000000 INFO @ Fri, 10 Dec 2021 17:39:35: 6000000 INFO @ Fri, 10 Dec 2021 17:39:36: 12000000 INFO @ Fri, 10 Dec 2021 17:39:42: 9000000 INFO @ Fri, 10 Dec 2021 17:39:45: 7000000 INFO @ Fri, 10 Dec 2021 17:39:46: 13000000 INFO @ Fri, 10 Dec 2021 17:39:52: 10000000 INFO @ Fri, 10 Dec 2021 17:39:55: 8000000 INFO @ Fri, 10 Dec 2021 17:39:55: 14000000 INFO @ Fri, 10 Dec 2021 17:40:03: 11000000 INFO @ Fri, 10 Dec 2021 17:40:05: 9000000 INFO @ Fri, 10 Dec 2021 17:40:05: 15000000 INFO @ Fri, 10 Dec 2021 17:40:14: 10000000 INFO @ Fri, 10 Dec 2021 17:40:14: 12000000 INFO @ Fri, 10 Dec 2021 17:40:15: 16000000 INFO @ Fri, 10 Dec 2021 17:40:24: 11000000 INFO @ Fri, 10 Dec 2021 17:40:25: 17000000 INFO @ Fri, 10 Dec 2021 17:40:25: 13000000 INFO @ Fri, 10 Dec 2021 17:40:34: 12000000 INFO @ Fri, 10 Dec 2021 17:40:35: 18000000 INFO @ Fri, 10 Dec 2021 17:40:36: 14000000 INFO @ Fri, 10 Dec 2021 17:40:44: 13000000 INFO @ Fri, 10 Dec 2021 17:40:45: 19000000 INFO @ Fri, 10 Dec 2021 17:40:47: 15000000 INFO @ Fri, 10 Dec 2021 17:40:55: 14000000 INFO @ Fri, 10 Dec 2021 17:40:55: 20000000 INFO @ Fri, 10 Dec 2021 17:40:58: 16000000 INFO @ Fri, 10 Dec 2021 17:41:04: 15000000 INFO @ Fri, 10 Dec 2021 17:41:06: 21000000 INFO @ Fri, 10 Dec 2021 17:41:08: 17000000 INFO @ Fri, 10 Dec 2021 17:41:14: 16000000 INFO @ Fri, 10 Dec 2021 17:41:17: 22000000 INFO @ Fri, 10 Dec 2021 17:41:19: 18000000 INFO @ Fri, 10 Dec 2021 17:41:24: 17000000 INFO @ Fri, 10 Dec 2021 17:41:27: 23000000 INFO @ Fri, 10 Dec 2021 17:41:30: 19000000 INFO @ Fri, 10 Dec 2021 17:41:34: 18000000 INFO @ Fri, 10 Dec 2021 17:41:37: 24000000 INFO @ Fri, 10 Dec 2021 17:41:41: 20000000 INFO @ Fri, 10 Dec 2021 17:41:44: 19000000 INFO @ Fri, 10 Dec 2021 17:41:48: 25000000 INFO @ Fri, 10 Dec 2021 17:41:53: 21000000 INFO @ Fri, 10 Dec 2021 17:41:54: 20000000 INFO @ Fri, 10 Dec 2021 17:41:57: 26000000 INFO @ Fri, 10 Dec 2021 17:42:05: 22000000 INFO @ Fri, 10 Dec 2021 17:42:05: 21000000 INFO @ Fri, 10 Dec 2021 17:42:07: 27000000 INFO @ Fri, 10 Dec 2021 17:42:16: 22000000 INFO @ Fri, 10 Dec 2021 17:42:17: 23000000 INFO @ Fri, 10 Dec 2021 17:42:18: 28000000 INFO @ Fri, 10 Dec 2021 17:42:27: 23000000 INFO @ Fri, 10 Dec 2021 17:42:28: 24000000 INFO @ Fri, 10 Dec 2021 17:42:29: 29000000 INFO @ Fri, 10 Dec 2021 17:42:37: 24000000 INFO @ Fri, 10 Dec 2021 17:42:39: 30000000 INFO @ Fri, 10 Dec 2021 17:42:39: 25000000 INFO @ Fri, 10 Dec 2021 17:42:48: 25000000 INFO @ Fri, 10 Dec 2021 17:42:50: 31000000 INFO @ Fri, 10 Dec 2021 17:42:50: 26000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 17:42:59: 26000000 INFO @ Fri, 10 Dec 2021 17:43:00: 32000000 INFO @ Fri, 10 Dec 2021 17:43:01: 27000000 INFO @ Fri, 10 Dec 2021 17:43:09: 27000000 INFO @ Fri, 10 Dec 2021 17:43:10: 33000000 INFO @ Fri, 10 Dec 2021 17:43:13: 28000000 INFO @ Fri, 10 Dec 2021 17:43:20: 28000000 INFO @ Fri, 10 Dec 2021 17:43:20: 34000000 INFO @ Fri, 10 Dec 2021 17:43:24: 29000000 INFO @ Fri, 10 Dec 2021 17:43:27: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 17:43:27: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 17:43:27: #1 total tags in treatment: 16858760 INFO @ Fri, 10 Dec 2021 17:43:27: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 17:43:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 17:43:28: #1 tags after filtering in treatment: 15873575 INFO @ Fri, 10 Dec 2021 17:43:28: #1 Redundant rate of treatment: 0.06 INFO @ Fri, 10 Dec 2021 17:43:28: #1 finished! INFO @ Fri, 10 Dec 2021 17:43:28: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 17:43:28: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 17:43:29: #2 number of paired peaks: 88 WARNING @ Fri, 10 Dec 2021 17:43:29: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 17:43:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7175257/SRX7175257.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175257/SRX7175257.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175257/SRX7175257.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175257/SRX7175257.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 17:43:30: 29000000 INFO @ Fri, 10 Dec 2021 17:43:35: 30000000 INFO @ Fri, 10 Dec 2021 17:43:39: 30000000 INFO @ Fri, 10 Dec 2021 17:43:45: 31000000 INFO @ Fri, 10 Dec 2021 17:43:49: 31000000 INFO @ Fri, 10 Dec 2021 17:43:56: 32000000 INFO @ Fri, 10 Dec 2021 17:43:59: 32000000 INFO @ Fri, 10 Dec 2021 17:44:08: 33000000 INFO @ Fri, 10 Dec 2021 17:44:09: 33000000 INFO @ Fri, 10 Dec 2021 17:44:18: 34000000 INFO @ Fri, 10 Dec 2021 17:44:22: 34000000 INFO @ Fri, 10 Dec 2021 17:44:26: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 17:44:26: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 17:44:26: #1 total tags in treatment: 16858760 INFO @ Fri, 10 Dec 2021 17:44:26: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 17:44:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 17:44:26: #1 tags after filtering in treatment: 15873575 INFO @ Fri, 10 Dec 2021 17:44:26: #1 Redundant rate of treatment: 0.06 INFO @ Fri, 10 Dec 2021 17:44:26: #1 finished! INFO @ Fri, 10 Dec 2021 17:44:26: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 17:44:26: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 17:44:27: #2 number of paired peaks: 88 WARNING @ Fri, 10 Dec 2021 17:44:27: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 17:44:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7175257/SRX7175257.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175257/SRX7175257.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175257/SRX7175257.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175257/SRX7175257.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 17:44:33: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 17:44:33: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 17:44:33: #1 total tags in treatment: 16858760 INFO @ Fri, 10 Dec 2021 17:44:33: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 17:44:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 17:44:33: #1 tags after filtering in treatment: 15873575 INFO @ Fri, 10 Dec 2021 17:44:33: #1 Redundant rate of treatment: 0.06 INFO @ Fri, 10 Dec 2021 17:44:33: #1 finished! INFO @ Fri, 10 Dec 2021 17:44:33: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 17:44:33: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 17:44:34: #2 number of paired peaks: 88 WARNING @ Fri, 10 Dec 2021 17:44:34: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 17:44:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7175257/SRX7175257.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175257/SRX7175257.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175257/SRX7175257.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175257/SRX7175257.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。