Job ID = 14168224 SRX = SRX7175254 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 29745592 spots for SRR10485694/SRR10485694.sra Written 29745592 spots for SRR10485694/SRR10485694.sra fastq に変換しました。 bowtie でマッピング中... Your job 14169314 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:05:23 29745592 reads; of these: 29745592 (100.00%) were paired; of these: 1480299 (4.98%) aligned concordantly 0 times 19211620 (64.59%) aligned concordantly exactly 1 time 9053673 (30.44%) aligned concordantly >1 times ---- 1480299 pairs aligned concordantly 0 times; of these: 185752 (12.55%) aligned discordantly 1 time ---- 1294547 pairs aligned 0 times concordantly or discordantly; of these: 2589094 mates make up the pairs; of these: 1412643 (54.56%) aligned 0 times 691799 (26.72%) aligned exactly 1 time 484652 (18.72%) aligned >1 times 97.63% overall alignment rate Time searching: 01:05:23 Overall time: 01:05:23 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 2306428 / 28382256 = 0.0813 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 17:34:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7175254/SRX7175254.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7175254/SRX7175254.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7175254/SRX7175254.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7175254/SRX7175254.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 17:34:55: #1 read tag files... INFO @ Fri, 10 Dec 2021 17:34:55: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 17:35:01: 1000000 INFO @ Fri, 10 Dec 2021 17:35:06: 2000000 INFO @ Fri, 10 Dec 2021 17:35:12: 3000000 INFO @ Fri, 10 Dec 2021 17:35:17: 4000000 INFO @ Fri, 10 Dec 2021 17:35:23: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 17:35:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7175254/SRX7175254.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7175254/SRX7175254.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7175254/SRX7175254.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7175254/SRX7175254.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 17:35:25: #1 read tag files... INFO @ Fri, 10 Dec 2021 17:35:25: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 17:35:29: 6000000 INFO @ Fri, 10 Dec 2021 17:35:31: 1000000 INFO @ Fri, 10 Dec 2021 17:35:35: 7000000 INFO @ Fri, 10 Dec 2021 17:35:37: 2000000 INFO @ Fri, 10 Dec 2021 17:35:41: 8000000 INFO @ Fri, 10 Dec 2021 17:35:44: 3000000 INFO @ Fri, 10 Dec 2021 17:35:48: 9000000 INFO @ Fri, 10 Dec 2021 17:35:50: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 17:35:54: 10000000 INFO @ Fri, 10 Dec 2021 17:35:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7175254/SRX7175254.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7175254/SRX7175254.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7175254/SRX7175254.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7175254/SRX7175254.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 17:35:55: #1 read tag files... INFO @ Fri, 10 Dec 2021 17:35:55: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 17:35:56: 5000000 INFO @ Fri, 10 Dec 2021 17:36:00: 11000000 INFO @ Fri, 10 Dec 2021 17:36:01: 1000000 INFO @ Fri, 10 Dec 2021 17:36:02: 6000000 INFO @ Fri, 10 Dec 2021 17:36:07: 12000000 INFO @ Fri, 10 Dec 2021 17:36:08: 2000000 INFO @ Fri, 10 Dec 2021 17:36:09: 7000000 INFO @ Fri, 10 Dec 2021 17:36:14: 13000000 INFO @ Fri, 10 Dec 2021 17:36:15: 3000000 INFO @ Fri, 10 Dec 2021 17:36:15: 8000000 INFO @ Fri, 10 Dec 2021 17:36:20: 14000000 INFO @ Fri, 10 Dec 2021 17:36:21: 9000000 INFO @ Fri, 10 Dec 2021 17:36:21: 4000000 INFO @ Fri, 10 Dec 2021 17:36:27: 15000000 INFO @ Fri, 10 Dec 2021 17:36:27: 10000000 INFO @ Fri, 10 Dec 2021 17:36:28: 5000000 INFO @ Fri, 10 Dec 2021 17:36:33: 16000000 INFO @ Fri, 10 Dec 2021 17:36:34: 11000000 INFO @ Fri, 10 Dec 2021 17:36:34: 6000000 INFO @ Fri, 10 Dec 2021 17:36:40: 17000000 INFO @ Fri, 10 Dec 2021 17:36:40: 12000000 INFO @ Fri, 10 Dec 2021 17:36:41: 7000000 INFO @ Fri, 10 Dec 2021 17:36:46: 18000000 INFO @ Fri, 10 Dec 2021 17:36:46: 13000000 INFO @ Fri, 10 Dec 2021 17:36:47: 8000000 INFO @ Fri, 10 Dec 2021 17:36:53: 14000000 INFO @ Fri, 10 Dec 2021 17:36:53: 19000000 INFO @ Fri, 10 Dec 2021 17:36:54: 9000000 INFO @ Fri, 10 Dec 2021 17:36:59: 15000000 INFO @ Fri, 10 Dec 2021 17:37:00: 20000000 INFO @ Fri, 10 Dec 2021 17:37:00: 10000000 INFO @ Fri, 10 Dec 2021 17:37:05: 16000000 INFO @ Fri, 10 Dec 2021 17:37:06: 21000000 INFO @ Fri, 10 Dec 2021 17:37:07: 11000000 INFO @ Fri, 10 Dec 2021 17:37:11: 17000000 INFO @ Fri, 10 Dec 2021 17:37:13: 22000000 INFO @ Fri, 10 Dec 2021 17:37:14: 12000000 INFO @ Fri, 10 Dec 2021 17:37:18: 18000000 INFO @ Fri, 10 Dec 2021 17:37:19: 23000000 INFO @ Fri, 10 Dec 2021 17:37:20: 13000000 INFO @ Fri, 10 Dec 2021 17:37:24: 19000000 INFO @ Fri, 10 Dec 2021 17:37:26: 24000000 INFO @ Fri, 10 Dec 2021 17:37:27: 14000000 INFO @ Fri, 10 Dec 2021 17:37:30: 20000000 INFO @ Fri, 10 Dec 2021 17:37:32: 25000000 INFO @ Fri, 10 Dec 2021 17:37:33: 15000000 INFO @ Fri, 10 Dec 2021 17:37:36: 21000000 INFO @ Fri, 10 Dec 2021 17:37:39: 26000000 INFO @ Fri, 10 Dec 2021 17:37:40: 16000000 INFO @ Fri, 10 Dec 2021 17:37:43: 22000000 INFO @ Fri, 10 Dec 2021 17:37:46: 27000000 INFO @ Fri, 10 Dec 2021 17:37:46: 17000000 INFO @ Fri, 10 Dec 2021 17:37:49: 23000000 INFO @ Fri, 10 Dec 2021 17:37:52: 28000000 INFO @ Fri, 10 Dec 2021 17:37:53: 18000000 INFO @ Fri, 10 Dec 2021 17:37:55: 24000000 INFO @ Fri, 10 Dec 2021 17:37:59: 29000000 INFO @ Fri, 10 Dec 2021 17:37:59: 19000000 INFO @ Fri, 10 Dec 2021 17:38:01: 25000000 INFO @ Fri, 10 Dec 2021 17:38:05: 30000000 INFO @ Fri, 10 Dec 2021 17:38:06: 20000000 INFO @ Fri, 10 Dec 2021 17:38:07: 26000000 INFO @ Fri, 10 Dec 2021 17:38:12: 31000000 INFO @ Fri, 10 Dec 2021 17:38:12: 21000000 INFO @ Fri, 10 Dec 2021 17:38:14: 27000000 INFO @ Fri, 10 Dec 2021 17:38:18: 32000000 INFO @ Fri, 10 Dec 2021 17:38:19: 22000000 INFO @ Fri, 10 Dec 2021 17:38:20: 28000000 INFO @ Fri, 10 Dec 2021 17:38:25: 33000000 INFO @ Fri, 10 Dec 2021 17:38:26: 23000000 INFO @ Fri, 10 Dec 2021 17:38:26: 29000000 INFO @ Fri, 10 Dec 2021 17:38:32: 34000000 INFO @ Fri, 10 Dec 2021 17:38:32: 24000000 INFO @ Fri, 10 Dec 2021 17:38:32: 30000000 INFO @ Fri, 10 Dec 2021 17:38:38: 35000000 INFO @ Fri, 10 Dec 2021 17:38:39: 31000000 INFO @ Fri, 10 Dec 2021 17:38:39: 25000000 INFO @ Fri, 10 Dec 2021 17:38:45: 36000000 INFO @ Fri, 10 Dec 2021 17:38:45: 32000000 INFO @ Fri, 10 Dec 2021 17:38:45: 26000000 INFO @ Fri, 10 Dec 2021 17:38:51: 37000000 INFO @ Fri, 10 Dec 2021 17:38:51: 33000000 INFO @ Fri, 10 Dec 2021 17:38:51: 27000000 INFO @ Fri, 10 Dec 2021 17:38:57: 38000000 INFO @ Fri, 10 Dec 2021 17:38:57: 34000000 INFO @ Fri, 10 Dec 2021 17:38:58: 28000000 INFO @ Fri, 10 Dec 2021 17:39:03: 39000000 INFO @ Fri, 10 Dec 2021 17:39:03: 35000000 INFO @ Fri, 10 Dec 2021 17:39:04: 29000000 INFO @ Fri, 10 Dec 2021 17:39:10: 36000000 INFO @ Fri, 10 Dec 2021 17:39:10: 40000000 INFO @ Fri, 10 Dec 2021 17:39:10: 30000000 INFO @ Fri, 10 Dec 2021 17:39:16: 37000000 INFO @ Fri, 10 Dec 2021 17:39:16: 41000000 INFO @ Fri, 10 Dec 2021 17:39:17: 31000000 INFO @ Fri, 10 Dec 2021 17:39:22: 38000000 INFO @ Fri, 10 Dec 2021 17:39:23: 42000000 INFO @ Fri, 10 Dec 2021 17:39:23: 32000000 INFO @ Fri, 10 Dec 2021 17:39:28: 39000000 INFO @ Fri, 10 Dec 2021 17:39:29: 43000000 INFO @ Fri, 10 Dec 2021 17:39:30: 33000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 17:39:34: 40000000 INFO @ Fri, 10 Dec 2021 17:39:35: 44000000 INFO @ Fri, 10 Dec 2021 17:39:36: 34000000 INFO @ Fri, 10 Dec 2021 17:39:40: 41000000 INFO @ Fri, 10 Dec 2021 17:39:42: 45000000 INFO @ Fri, 10 Dec 2021 17:39:42: 35000000 INFO @ Fri, 10 Dec 2021 17:39:46: 42000000 INFO @ Fri, 10 Dec 2021 17:39:48: 46000000 INFO @ Fri, 10 Dec 2021 17:39:49: 36000000 INFO @ Fri, 10 Dec 2021 17:39:53: 43000000 INFO @ Fri, 10 Dec 2021 17:39:55: 47000000 INFO @ Fri, 10 Dec 2021 17:39:55: 37000000 INFO @ Fri, 10 Dec 2021 17:39:59: 44000000 INFO @ Fri, 10 Dec 2021 17:40:01: 48000000 INFO @ Fri, 10 Dec 2021 17:40:01: 38000000 INFO @ Fri, 10 Dec 2021 17:40:05: 45000000 INFO @ Fri, 10 Dec 2021 17:40:07: 49000000 INFO @ Fri, 10 Dec 2021 17:40:07: 39000000 INFO @ Fri, 10 Dec 2021 17:40:11: 46000000 INFO @ Fri, 10 Dec 2021 17:40:14: 50000000 INFO @ Fri, 10 Dec 2021 17:40:14: 40000000 INFO @ Fri, 10 Dec 2021 17:40:17: 47000000 INFO @ Fri, 10 Dec 2021 17:40:20: 51000000 INFO @ Fri, 10 Dec 2021 17:40:20: 41000000 INFO @ Fri, 10 Dec 2021 17:40:24: 48000000 INFO @ Fri, 10 Dec 2021 17:40:26: 52000000 INFO @ Fri, 10 Dec 2021 17:40:27: 42000000 INFO @ Fri, 10 Dec 2021 17:40:30: 49000000 INFO @ Fri, 10 Dec 2021 17:40:33: 53000000 INFO @ Fri, 10 Dec 2021 17:40:33: 43000000 INFO @ Fri, 10 Dec 2021 17:40:36: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 17:40:36: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 17:40:36: #1 total tags in treatment: 25963661 INFO @ Fri, 10 Dec 2021 17:40:36: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 17:40:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 17:40:36: 50000000 INFO @ Fri, 10 Dec 2021 17:40:36: #1 tags after filtering in treatment: 23299816 INFO @ Fri, 10 Dec 2021 17:40:36: #1 Redundant rate of treatment: 0.10 INFO @ Fri, 10 Dec 2021 17:40:36: #1 finished! INFO @ Fri, 10 Dec 2021 17:40:36: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 17:40:36: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 17:40:38: #2 number of paired peaks: 80 WARNING @ Fri, 10 Dec 2021 17:40:38: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 17:40:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7175254/SRX7175254.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175254/SRX7175254.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175254/SRX7175254.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175254/SRX7175254.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 17:40:40: 44000000 INFO @ Fri, 10 Dec 2021 17:40:42: 51000000 INFO @ Fri, 10 Dec 2021 17:40:46: 45000000 INFO @ Fri, 10 Dec 2021 17:40:48: 52000000 INFO @ Fri, 10 Dec 2021 17:40:52: 46000000 INFO @ Fri, 10 Dec 2021 17:40:54: 53000000 INFO @ Fri, 10 Dec 2021 17:40:57: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 17:40:57: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 17:40:57: #1 total tags in treatment: 25963661 INFO @ Fri, 10 Dec 2021 17:40:57: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 17:40:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 17:40:58: #1 tags after filtering in treatment: 23299816 INFO @ Fri, 10 Dec 2021 17:40:58: #1 Redundant rate of treatment: 0.10 INFO @ Fri, 10 Dec 2021 17:40:58: #1 finished! INFO @ Fri, 10 Dec 2021 17:40:58: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 17:40:58: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 17:40:58: 47000000 INFO @ Fri, 10 Dec 2021 17:40:59: #2 number of paired peaks: 80 WARNING @ Fri, 10 Dec 2021 17:40:59: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 17:40:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7175254/SRX7175254.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175254/SRX7175254.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175254/SRX7175254.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175254/SRX7175254.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 17:41:04: 48000000 INFO @ Fri, 10 Dec 2021 17:41:10: 49000000 INFO @ Fri, 10 Dec 2021 17:41:16: 50000000 INFO @ Fri, 10 Dec 2021 17:41:22: 51000000 INFO @ Fri, 10 Dec 2021 17:41:27: 52000000 INFO @ Fri, 10 Dec 2021 17:41:33: 53000000 INFO @ Fri, 10 Dec 2021 17:41:36: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 17:41:36: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 17:41:36: #1 total tags in treatment: 25963661 INFO @ Fri, 10 Dec 2021 17:41:36: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 17:41:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 17:41:36: #1 tags after filtering in treatment: 23299816 INFO @ Fri, 10 Dec 2021 17:41:36: #1 Redundant rate of treatment: 0.10 INFO @ Fri, 10 Dec 2021 17:41:36: #1 finished! INFO @ Fri, 10 Dec 2021 17:41:36: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 17:41:36: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 17:41:38: #2 number of paired peaks: 80 WARNING @ Fri, 10 Dec 2021 17:41:38: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 17:41:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7175254/SRX7175254.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175254/SRX7175254.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175254/SRX7175254.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175254/SRX7175254.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling