Job ID = 14168150 SRX = SRX7175248 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 21065616 spots for SRR10485688/SRR10485688.sra Written 21065616 spots for SRR10485688/SRR10485688.sra fastq に変換しました。 bowtie でマッピング中... Your job 14169137 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:05:30 21065616 reads; of these: 21065616 (100.00%) were paired; of these: 1138280 (5.40%) aligned concordantly 0 times 13474974 (63.97%) aligned concordantly exactly 1 time 6452362 (30.63%) aligned concordantly >1 times ---- 1138280 pairs aligned concordantly 0 times; of these: 86139 (7.57%) aligned discordantly 1 time ---- 1052141 pairs aligned 0 times concordantly or discordantly; of these: 2104282 mates make up the pairs; of these: 1229854 (58.45%) aligned 0 times 477246 (22.68%) aligned exactly 1 time 397182 (18.87%) aligned >1 times 97.08% overall alignment rate Time searching: 01:05:30 Overall time: 01:05:30 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 1094600 / 19978406 = 0.0548 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 17:05:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7175248/SRX7175248.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7175248/SRX7175248.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7175248/SRX7175248.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7175248/SRX7175248.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 17:05:38: #1 read tag files... INFO @ Fri, 10 Dec 2021 17:05:38: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 17:05:44: 1000000 INFO @ Fri, 10 Dec 2021 17:05:49: 2000000 INFO @ Fri, 10 Dec 2021 17:05:54: 3000000 INFO @ Fri, 10 Dec 2021 17:05:59: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 17:06:05: 5000000 INFO @ Fri, 10 Dec 2021 17:06:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7175248/SRX7175248.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7175248/SRX7175248.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7175248/SRX7175248.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7175248/SRX7175248.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 17:06:07: #1 read tag files... INFO @ Fri, 10 Dec 2021 17:06:07: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 17:06:10: 6000000 INFO @ Fri, 10 Dec 2021 17:06:13: 1000000 INFO @ Fri, 10 Dec 2021 17:06:15: 7000000 INFO @ Fri, 10 Dec 2021 17:06:20: 2000000 INFO @ Fri, 10 Dec 2021 17:06:20: 8000000 INFO @ Fri, 10 Dec 2021 17:06:25: 9000000 INFO @ Fri, 10 Dec 2021 17:06:26: 3000000 INFO @ Fri, 10 Dec 2021 17:06:31: 10000000 INFO @ Fri, 10 Dec 2021 17:06:33: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 17:06:36: 11000000 INFO @ Fri, 10 Dec 2021 17:06:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7175248/SRX7175248.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7175248/SRX7175248.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7175248/SRX7175248.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7175248/SRX7175248.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 17:06:37: #1 read tag files... INFO @ Fri, 10 Dec 2021 17:06:37: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 17:06:39: 5000000 INFO @ Fri, 10 Dec 2021 17:06:41: 12000000 INFO @ Fri, 10 Dec 2021 17:06:44: 1000000 INFO @ Fri, 10 Dec 2021 17:06:46: 6000000 INFO @ Fri, 10 Dec 2021 17:06:46: 13000000 INFO @ Fri, 10 Dec 2021 17:06:51: 2000000 INFO @ Fri, 10 Dec 2021 17:06:51: 14000000 INFO @ Fri, 10 Dec 2021 17:06:53: 7000000 INFO @ Fri, 10 Dec 2021 17:06:57: 15000000 INFO @ Fri, 10 Dec 2021 17:06:58: 3000000 INFO @ Fri, 10 Dec 2021 17:07:00: 8000000 INFO @ Fri, 10 Dec 2021 17:07:02: 16000000 INFO @ Fri, 10 Dec 2021 17:07:05: 4000000 INFO @ Fri, 10 Dec 2021 17:07:07: 17000000 INFO @ Fri, 10 Dec 2021 17:07:07: 9000000 INFO @ Fri, 10 Dec 2021 17:07:12: 5000000 INFO @ Fri, 10 Dec 2021 17:07:12: 18000000 INFO @ Fri, 10 Dec 2021 17:07:14: 10000000 INFO @ Fri, 10 Dec 2021 17:07:17: 19000000 INFO @ Fri, 10 Dec 2021 17:07:19: 6000000 INFO @ Fri, 10 Dec 2021 17:07:21: 11000000 INFO @ Fri, 10 Dec 2021 17:07:22: 20000000 INFO @ Fri, 10 Dec 2021 17:07:26: 7000000 INFO @ Fri, 10 Dec 2021 17:07:27: 21000000 INFO @ Fri, 10 Dec 2021 17:07:28: 12000000 INFO @ Fri, 10 Dec 2021 17:07:33: 8000000 INFO @ Fri, 10 Dec 2021 17:07:33: 22000000 INFO @ Fri, 10 Dec 2021 17:07:35: 13000000 INFO @ Fri, 10 Dec 2021 17:07:38: 23000000 INFO @ Fri, 10 Dec 2021 17:07:40: 9000000 INFO @ Fri, 10 Dec 2021 17:07:42: 14000000 INFO @ Fri, 10 Dec 2021 17:07:43: 24000000 INFO @ Fri, 10 Dec 2021 17:07:46: 10000000 INFO @ Fri, 10 Dec 2021 17:07:48: 25000000 INFO @ Fri, 10 Dec 2021 17:07:49: 15000000 INFO @ Fri, 10 Dec 2021 17:07:53: 26000000 INFO @ Fri, 10 Dec 2021 17:07:53: 11000000 INFO @ Fri, 10 Dec 2021 17:07:56: 16000000 INFO @ Fri, 10 Dec 2021 17:07:58: 27000000 INFO @ Fri, 10 Dec 2021 17:08:00: 12000000 INFO @ Fri, 10 Dec 2021 17:08:02: 17000000 INFO @ Fri, 10 Dec 2021 17:08:03: 28000000 INFO @ Fri, 10 Dec 2021 17:08:07: 13000000 INFO @ Fri, 10 Dec 2021 17:08:08: 29000000 INFO @ Fri, 10 Dec 2021 17:08:09: 18000000 INFO @ Fri, 10 Dec 2021 17:08:14: 30000000 INFO @ Fri, 10 Dec 2021 17:08:14: 14000000 INFO @ Fri, 10 Dec 2021 17:08:16: 19000000 INFO @ Fri, 10 Dec 2021 17:08:19: 31000000 INFO @ Fri, 10 Dec 2021 17:08:21: 15000000 INFO @ Fri, 10 Dec 2021 17:08:23: 20000000 INFO @ Fri, 10 Dec 2021 17:08:24: 32000000 INFO @ Fri, 10 Dec 2021 17:08:28: 16000000 INFO @ Fri, 10 Dec 2021 17:08:29: 33000000 INFO @ Fri, 10 Dec 2021 17:08:30: 21000000 INFO @ Fri, 10 Dec 2021 17:08:34: 34000000 INFO @ Fri, 10 Dec 2021 17:08:35: 17000000 INFO @ Fri, 10 Dec 2021 17:08:37: 22000000 INFO @ Fri, 10 Dec 2021 17:08:40: 35000000 INFO @ Fri, 10 Dec 2021 17:08:41: 18000000 INFO @ Fri, 10 Dec 2021 17:08:44: 23000000 INFO @ Fri, 10 Dec 2021 17:08:45: 36000000 INFO @ Fri, 10 Dec 2021 17:08:48: 19000000 INFO @ Fri, 10 Dec 2021 17:08:50: 37000000 INFO @ Fri, 10 Dec 2021 17:08:50: 24000000 INFO @ Fri, 10 Dec 2021 17:08:55: 20000000 INFO @ Fri, 10 Dec 2021 17:08:55: 38000000 INFO @ Fri, 10 Dec 2021 17:08:57: 25000000 INFO @ Fri, 10 Dec 2021 17:08:59: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 17:08:59: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 17:08:59: #1 total tags in treatment: 18834074 INFO @ Fri, 10 Dec 2021 17:08:59: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 17:08:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 17:08:59: #1 tags after filtering in treatment: 16999364 INFO @ Fri, 10 Dec 2021 17:08:59: #1 Redundant rate of treatment: 0.10 INFO @ Fri, 10 Dec 2021 17:08:59: #1 finished! INFO @ Fri, 10 Dec 2021 17:08:59: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 17:08:59: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 17:09:01: #2 number of paired peaks: 84 WARNING @ Fri, 10 Dec 2021 17:09:01: Too few paired peaks (84) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 17:09:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7175248/SRX7175248.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175248/SRX7175248.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175248/SRX7175248.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175248/SRX7175248.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 17:09:02: 21000000 INFO @ Fri, 10 Dec 2021 17:09:04: 26000000 INFO @ Fri, 10 Dec 2021 17:09:09: 22000000 INFO @ Fri, 10 Dec 2021 17:09:10: 27000000 INFO @ Fri, 10 Dec 2021 17:09:15: 23000000 INFO @ Fri, 10 Dec 2021 17:09:17: 28000000 INFO @ Fri, 10 Dec 2021 17:09:22: 24000000 INFO @ Fri, 10 Dec 2021 17:09:24: 29000000 INFO @ Fri, 10 Dec 2021 17:09:29: 25000000 INFO @ Fri, 10 Dec 2021 17:09:30: 30000000 INFO @ Fri, 10 Dec 2021 17:09:35: 26000000 INFO @ Fri, 10 Dec 2021 17:09:37: 31000000 INFO @ Fri, 10 Dec 2021 17:09:42: 27000000 INFO @ Fri, 10 Dec 2021 17:09:44: 32000000 INFO @ Fri, 10 Dec 2021 17:09:48: 28000000 INFO @ Fri, 10 Dec 2021 17:09:51: 33000000 INFO @ Fri, 10 Dec 2021 17:09:55: 29000000 INFO @ Fri, 10 Dec 2021 17:09:58: 34000000 INFO @ Fri, 10 Dec 2021 17:10:02: 30000000 INFO @ Fri, 10 Dec 2021 17:10:05: 35000000 INFO @ Fri, 10 Dec 2021 17:10:09: 31000000 INFO @ Fri, 10 Dec 2021 17:10:11: 36000000 INFO @ Fri, 10 Dec 2021 17:10:15: 32000000 INFO @ Fri, 10 Dec 2021 17:10:18: 37000000 INFO @ Fri, 10 Dec 2021 17:10:22: 33000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 17:10:25: 38000000 INFO @ Fri, 10 Dec 2021 17:10:29: 34000000 INFO @ Fri, 10 Dec 2021 17:10:30: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 17:10:30: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 17:10:30: #1 total tags in treatment: 18834074 INFO @ Fri, 10 Dec 2021 17:10:30: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 17:10:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 17:10:30: #1 tags after filtering in treatment: 16999364 INFO @ Fri, 10 Dec 2021 17:10:30: #1 Redundant rate of treatment: 0.10 INFO @ Fri, 10 Dec 2021 17:10:30: #1 finished! INFO @ Fri, 10 Dec 2021 17:10:30: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 17:10:30: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 17:10:31: #2 number of paired peaks: 84 WARNING @ Fri, 10 Dec 2021 17:10:31: Too few paired peaks (84) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 17:10:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7175248/SRX7175248.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175248/SRX7175248.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175248/SRX7175248.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175248/SRX7175248.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 17:10:35: 35000000 INFO @ Fri, 10 Dec 2021 17:10:41: 36000000 INFO @ Fri, 10 Dec 2021 17:10:47: 37000000 INFO @ Fri, 10 Dec 2021 17:10:54: 38000000 INFO @ Fri, 10 Dec 2021 17:10:58: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 17:10:58: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 17:10:58: #1 total tags in treatment: 18834074 INFO @ Fri, 10 Dec 2021 17:10:58: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 17:10:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 17:10:58: #1 tags after filtering in treatment: 16999364 INFO @ Fri, 10 Dec 2021 17:10:58: #1 Redundant rate of treatment: 0.10 INFO @ Fri, 10 Dec 2021 17:10:58: #1 finished! INFO @ Fri, 10 Dec 2021 17:10:58: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 17:10:58: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 17:11:00: #2 number of paired peaks: 84 WARNING @ Fri, 10 Dec 2021 17:11:00: Too few paired peaks (84) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 17:11:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7175248/SRX7175248.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175248/SRX7175248.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175248/SRX7175248.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175248/SRX7175248.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。