Job ID = 14168134 SRX = SRX7175242 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 32036737 spots for SRR10485682/SRR10485682.sra Written 32036737 spots for SRR10485682/SRR10485682.sra fastq に変換しました。 bowtie でマッピング中... Your job 14169164 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:19:47 32036737 reads; of these: 32036737 (100.00%) were paired; of these: 4639841 (14.48%) aligned concordantly 0 times 20197715 (63.05%) aligned concordantly exactly 1 time 7199181 (22.47%) aligned concordantly >1 times ---- 4639841 pairs aligned concordantly 0 times; of these: 191818 (4.13%) aligned discordantly 1 time ---- 4448023 pairs aligned 0 times concordantly or discordantly; of these: 8896046 mates make up the pairs; of these: 7016795 (78.88%) aligned 0 times 938386 (10.55%) aligned exactly 1 time 940865 (10.58%) aligned >1 times 89.05% overall alignment rate Time searching: 01:19:48 Overall time: 01:19:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 3091906 / 27489665 = 0.1125 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 17:16:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7175242/SRX7175242.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7175242/SRX7175242.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7175242/SRX7175242.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7175242/SRX7175242.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 17:16:37: #1 read tag files... INFO @ Fri, 10 Dec 2021 17:16:37: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 17:16:43: 1000000 INFO @ Fri, 10 Dec 2021 17:16:48: 2000000 INFO @ Fri, 10 Dec 2021 17:16:53: 3000000 INFO @ Fri, 10 Dec 2021 17:16:58: 4000000 INFO @ Fri, 10 Dec 2021 17:17:04: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 17:17:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7175242/SRX7175242.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7175242/SRX7175242.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7175242/SRX7175242.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7175242/SRX7175242.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 17:17:07: #1 read tag files... INFO @ Fri, 10 Dec 2021 17:17:07: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 17:17:09: 6000000 INFO @ Fri, 10 Dec 2021 17:17:13: 1000000 INFO @ Fri, 10 Dec 2021 17:17:15: 7000000 INFO @ Fri, 10 Dec 2021 17:17:18: 2000000 INFO @ Fri, 10 Dec 2021 17:17:20: 8000000 INFO @ Fri, 10 Dec 2021 17:17:23: 3000000 INFO @ Fri, 10 Dec 2021 17:17:25: 9000000 INFO @ Fri, 10 Dec 2021 17:17:29: 4000000 INFO @ Fri, 10 Dec 2021 17:17:31: 10000000 INFO @ Fri, 10 Dec 2021 17:17:34: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 17:17:36: 11000000 INFO @ Fri, 10 Dec 2021 17:17:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7175242/SRX7175242.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7175242/SRX7175242.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7175242/SRX7175242.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7175242/SRX7175242.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 17:17:37: #1 read tag files... INFO @ Fri, 10 Dec 2021 17:17:37: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 17:17:40: 6000000 INFO @ Fri, 10 Dec 2021 17:17:42: 12000000 INFO @ Fri, 10 Dec 2021 17:17:44: 1000000 INFO @ Fri, 10 Dec 2021 17:17:45: 7000000 INFO @ Fri, 10 Dec 2021 17:17:47: 13000000 INFO @ Fri, 10 Dec 2021 17:17:50: 2000000 INFO @ Fri, 10 Dec 2021 17:17:50: 8000000 INFO @ Fri, 10 Dec 2021 17:17:53: 14000000 INFO @ Fri, 10 Dec 2021 17:17:56: 9000000 INFO @ Fri, 10 Dec 2021 17:17:56: 3000000 INFO @ Fri, 10 Dec 2021 17:17:58: 15000000 INFO @ Fri, 10 Dec 2021 17:18:01: 10000000 INFO @ Fri, 10 Dec 2021 17:18:03: 4000000 INFO @ Fri, 10 Dec 2021 17:18:04: 16000000 INFO @ Fri, 10 Dec 2021 17:18:07: 11000000 INFO @ Fri, 10 Dec 2021 17:18:09: 5000000 INFO @ Fri, 10 Dec 2021 17:18:09: 17000000 INFO @ Fri, 10 Dec 2021 17:18:12: 12000000 INFO @ Fri, 10 Dec 2021 17:18:15: 18000000 INFO @ Fri, 10 Dec 2021 17:18:15: 6000000 INFO @ Fri, 10 Dec 2021 17:18:18: 13000000 INFO @ Fri, 10 Dec 2021 17:18:20: 19000000 INFO @ Fri, 10 Dec 2021 17:18:22: 7000000 INFO @ Fri, 10 Dec 2021 17:18:23: 14000000 INFO @ Fri, 10 Dec 2021 17:18:26: 20000000 INFO @ Fri, 10 Dec 2021 17:18:28: 8000000 INFO @ Fri, 10 Dec 2021 17:18:29: 15000000 INFO @ Fri, 10 Dec 2021 17:18:31: 21000000 INFO @ Fri, 10 Dec 2021 17:18:34: 16000000 INFO @ Fri, 10 Dec 2021 17:18:34: 9000000 INFO @ Fri, 10 Dec 2021 17:18:37: 22000000 INFO @ Fri, 10 Dec 2021 17:18:40: 17000000 INFO @ Fri, 10 Dec 2021 17:18:41: 10000000 INFO @ Fri, 10 Dec 2021 17:18:42: 23000000 INFO @ Fri, 10 Dec 2021 17:18:45: 18000000 INFO @ Fri, 10 Dec 2021 17:18:47: 11000000 INFO @ Fri, 10 Dec 2021 17:18:47: 24000000 INFO @ Fri, 10 Dec 2021 17:18:50: 19000000 INFO @ Fri, 10 Dec 2021 17:18:53: 25000000 INFO @ Fri, 10 Dec 2021 17:18:54: 12000000 INFO @ Fri, 10 Dec 2021 17:18:56: 20000000 INFO @ Fri, 10 Dec 2021 17:18:58: 26000000 INFO @ Fri, 10 Dec 2021 17:19:00: 13000000 INFO @ Fri, 10 Dec 2021 17:19:01: 21000000 INFO @ Fri, 10 Dec 2021 17:19:04: 27000000 INFO @ Fri, 10 Dec 2021 17:19:06: 14000000 INFO @ Fri, 10 Dec 2021 17:19:07: 22000000 INFO @ Fri, 10 Dec 2021 17:19:10: 28000000 INFO @ Fri, 10 Dec 2021 17:19:12: 23000000 INFO @ Fri, 10 Dec 2021 17:19:13: 15000000 INFO @ Fri, 10 Dec 2021 17:19:15: 29000000 INFO @ Fri, 10 Dec 2021 17:19:17: 24000000 INFO @ Fri, 10 Dec 2021 17:19:19: 16000000 INFO @ Fri, 10 Dec 2021 17:19:21: 30000000 INFO @ Fri, 10 Dec 2021 17:19:23: 25000000 INFO @ Fri, 10 Dec 2021 17:19:25: 17000000 INFO @ Fri, 10 Dec 2021 17:19:26: 31000000 INFO @ Fri, 10 Dec 2021 17:19:28: 26000000 INFO @ Fri, 10 Dec 2021 17:19:32: 32000000 INFO @ Fri, 10 Dec 2021 17:19:32: 18000000 INFO @ Fri, 10 Dec 2021 17:19:34: 27000000 INFO @ Fri, 10 Dec 2021 17:19:37: 33000000 INFO @ Fri, 10 Dec 2021 17:19:38: 19000000 INFO @ Fri, 10 Dec 2021 17:19:39: 28000000 INFO @ Fri, 10 Dec 2021 17:19:43: 34000000 INFO @ Fri, 10 Dec 2021 17:19:44: 20000000 INFO @ Fri, 10 Dec 2021 17:19:44: 29000000 INFO @ Fri, 10 Dec 2021 17:19:48: 35000000 INFO @ Fri, 10 Dec 2021 17:19:50: 30000000 INFO @ Fri, 10 Dec 2021 17:19:51: 21000000 INFO @ Fri, 10 Dec 2021 17:19:54: 36000000 INFO @ Fri, 10 Dec 2021 17:19:55: 31000000 INFO @ Fri, 10 Dec 2021 17:19:57: 22000000 INFO @ Fri, 10 Dec 2021 17:20:00: 37000000 INFO @ Fri, 10 Dec 2021 17:20:01: 32000000 INFO @ Fri, 10 Dec 2021 17:20:03: 23000000 INFO @ Fri, 10 Dec 2021 17:20:05: 38000000 INFO @ Fri, 10 Dec 2021 17:20:06: 33000000 INFO @ Fri, 10 Dec 2021 17:20:10: 24000000 INFO @ Fri, 10 Dec 2021 17:20:11: 39000000 INFO @ Fri, 10 Dec 2021 17:20:12: 34000000 INFO @ Fri, 10 Dec 2021 17:20:16: 25000000 INFO @ Fri, 10 Dec 2021 17:20:16: 40000000 INFO @ Fri, 10 Dec 2021 17:20:17: 35000000 INFO @ Fri, 10 Dec 2021 17:20:22: 41000000 INFO @ Fri, 10 Dec 2021 17:20:22: 26000000 INFO @ Fri, 10 Dec 2021 17:20:23: 36000000 INFO @ Fri, 10 Dec 2021 17:20:28: 42000000 INFO @ Fri, 10 Dec 2021 17:20:28: 37000000 INFO @ Fri, 10 Dec 2021 17:20:29: 27000000 INFO @ Fri, 10 Dec 2021 17:20:33: 43000000 INFO @ Fri, 10 Dec 2021 17:20:34: 38000000 INFO @ Fri, 10 Dec 2021 17:20:35: 28000000 INFO @ Fri, 10 Dec 2021 17:20:39: 44000000 INFO @ Fri, 10 Dec 2021 17:20:39: 39000000 INFO @ Fri, 10 Dec 2021 17:20:41: 29000000 INFO @ Fri, 10 Dec 2021 17:20:44: 45000000 INFO @ Fri, 10 Dec 2021 17:20:45: 40000000 INFO @ Fri, 10 Dec 2021 17:20:48: 30000000 INFO @ Fri, 10 Dec 2021 17:20:50: 46000000 INFO @ Fri, 10 Dec 2021 17:20:50: 41000000 INFO @ Fri, 10 Dec 2021 17:20:54: 31000000 INFO @ Fri, 10 Dec 2021 17:20:56: 47000000 INFO @ Fri, 10 Dec 2021 17:20:56: 42000000 INFO @ Fri, 10 Dec 2021 17:21:00: 32000000 INFO @ Fri, 10 Dec 2021 17:21:01: 43000000 INFO @ Fri, 10 Dec 2021 17:21:01: 48000000 INFO @ Fri, 10 Dec 2021 17:21:07: 44000000 INFO @ Fri, 10 Dec 2021 17:21:07: 49000000 INFO @ Fri, 10 Dec 2021 17:21:07: 33000000 INFO @ Fri, 10 Dec 2021 17:21:12: 45000000 INFO @ Fri, 10 Dec 2021 17:21:12: 50000000 INFO @ Fri, 10 Dec 2021 17:21:13: 34000000 INFO @ Fri, 10 Dec 2021 17:21:18: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 17:21:18: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 17:21:18: #1 total tags in treatment: 24311237 INFO @ Fri, 10 Dec 2021 17:21:18: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 17:21:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 17:21:18: 46000000 INFO @ Fri, 10 Dec 2021 17:21:18: #1 tags after filtering in treatment: 22593500 INFO @ Fri, 10 Dec 2021 17:21:18: #1 Redundant rate of treatment: 0.07 INFO @ Fri, 10 Dec 2021 17:21:18: #1 finished! INFO @ Fri, 10 Dec 2021 17:21:18: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 17:21:18: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 17:21:20: 35000000 INFO @ Fri, 10 Dec 2021 17:21:20: #2 number of paired peaks: 82 WARNING @ Fri, 10 Dec 2021 17:21:20: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 17:21:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7175242/SRX7175242.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175242/SRX7175242.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175242/SRX7175242.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175242/SRX7175242.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 17:21:23: 47000000 INFO @ Fri, 10 Dec 2021 17:21:26: 36000000 INFO @ Fri, 10 Dec 2021 17:21:29: 48000000 INFO @ Fri, 10 Dec 2021 17:21:32: 37000000 INFO @ Fri, 10 Dec 2021 17:21:34: 49000000 INFO @ Fri, 10 Dec 2021 17:21:39: 38000000 INFO @ Fri, 10 Dec 2021 17:21:40: 50000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 17:21:45: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 17:21:45: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 17:21:45: #1 total tags in treatment: 24311237 INFO @ Fri, 10 Dec 2021 17:21:45: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 17:21:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 17:21:45: #1 tags after filtering in treatment: 22593500 INFO @ Fri, 10 Dec 2021 17:21:45: #1 Redundant rate of treatment: 0.07 INFO @ Fri, 10 Dec 2021 17:21:45: #1 finished! INFO @ Fri, 10 Dec 2021 17:21:45: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 17:21:45: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 17:21:45: 39000000 INFO @ Fri, 10 Dec 2021 17:21:47: #2 number of paired peaks: 82 WARNING @ Fri, 10 Dec 2021 17:21:47: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 17:21:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7175242/SRX7175242.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175242/SRX7175242.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175242/SRX7175242.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175242/SRX7175242.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 17:21:51: 40000000 INFO @ Fri, 10 Dec 2021 17:21:58: 41000000 INFO @ Fri, 10 Dec 2021 17:22:04: 42000000 INFO @ Fri, 10 Dec 2021 17:22:10: 43000000 INFO @ Fri, 10 Dec 2021 17:22:17: 44000000 INFO @ Fri, 10 Dec 2021 17:22:23: 45000000 INFO @ Fri, 10 Dec 2021 17:22:29: 46000000 INFO @ Fri, 10 Dec 2021 17:22:35: 47000000 INFO @ Fri, 10 Dec 2021 17:22:42: 48000000 INFO @ Fri, 10 Dec 2021 17:22:48: 49000000 INFO @ Fri, 10 Dec 2021 17:22:54: 50000000 INFO @ Fri, 10 Dec 2021 17:23:00: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 17:23:00: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 17:23:00: #1 total tags in treatment: 24311237 INFO @ Fri, 10 Dec 2021 17:23:00: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 17:23:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 17:23:00: #1 tags after filtering in treatment: 22593500 INFO @ Fri, 10 Dec 2021 17:23:00: #1 Redundant rate of treatment: 0.07 INFO @ Fri, 10 Dec 2021 17:23:00: #1 finished! INFO @ Fri, 10 Dec 2021 17:23:00: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 17:23:00: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 17:23:02: #2 number of paired peaks: 82 WARNING @ Fri, 10 Dec 2021 17:23:02: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 17:23:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7175242/SRX7175242.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175242/SRX7175242.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175242/SRX7175242.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175242/SRX7175242.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。