Job ID = 14168053 SRX = SRX7175238 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 26942224 spots for SRR10485678/SRR10485678.sra Written 26942224 spots for SRR10485678/SRR10485678.sra fastq に変換しました。 bowtie でマッピング中... Your job 14168951 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:59:20 26942224 reads; of these: 26942224 (100.00%) were paired; of these: 2191494 (8.13%) aligned concordantly 0 times 17951041 (66.63%) aligned concordantly exactly 1 time 6799689 (25.24%) aligned concordantly >1 times ---- 2191494 pairs aligned concordantly 0 times; of these: 381655 (17.42%) aligned discordantly 1 time ---- 1809839 pairs aligned 0 times concordantly or discordantly; of these: 3619678 mates make up the pairs; of these: 2011283 (55.57%) aligned 0 times 837508 (23.14%) aligned exactly 1 time 770887 (21.30%) aligned >1 times 96.27% overall alignment rate Time searching: 00:59:20 Overall time: 00:59:20 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 1319043 / 24897240 = 0.0530 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 16:01:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7175238/SRX7175238.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7175238/SRX7175238.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7175238/SRX7175238.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7175238/SRX7175238.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 16:01:15: #1 read tag files... INFO @ Fri, 10 Dec 2021 16:01:15: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 16:01:21: 1000000 INFO @ Fri, 10 Dec 2021 16:01:26: 2000000 INFO @ Fri, 10 Dec 2021 16:01:32: 3000000 INFO @ Fri, 10 Dec 2021 16:01:38: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 16:01:44: 5000000 INFO @ Fri, 10 Dec 2021 16:01:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7175238/SRX7175238.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7175238/SRX7175238.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7175238/SRX7175238.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7175238/SRX7175238.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 16:01:45: #1 read tag files... INFO @ Fri, 10 Dec 2021 16:01:45: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 16:01:51: 6000000 INFO @ Fri, 10 Dec 2021 16:01:52: 1000000 INFO @ Fri, 10 Dec 2021 16:01:58: 7000000 INFO @ Fri, 10 Dec 2021 16:01:59: 2000000 INFO @ Fri, 10 Dec 2021 16:02:04: 8000000 INFO @ Fri, 10 Dec 2021 16:02:06: 3000000 INFO @ Fri, 10 Dec 2021 16:02:11: 9000000 INFO @ Fri, 10 Dec 2021 16:02:13: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 16:02:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7175238/SRX7175238.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7175238/SRX7175238.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7175238/SRX7175238.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7175238/SRX7175238.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 16:02:15: #1 read tag files... INFO @ Fri, 10 Dec 2021 16:02:15: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 16:02:19: 10000000 INFO @ Fri, 10 Dec 2021 16:02:21: 5000000 INFO @ Fri, 10 Dec 2021 16:02:23: 1000000 INFO @ Fri, 10 Dec 2021 16:02:27: 11000000 INFO @ Fri, 10 Dec 2021 16:02:29: 6000000 INFO @ Fri, 10 Dec 2021 16:02:32: 2000000 INFO @ Fri, 10 Dec 2021 16:02:35: 12000000 INFO @ Fri, 10 Dec 2021 16:02:37: 7000000 INFO @ Fri, 10 Dec 2021 16:02:41: 3000000 INFO @ Fri, 10 Dec 2021 16:02:43: 13000000 INFO @ Fri, 10 Dec 2021 16:02:45: 8000000 INFO @ Fri, 10 Dec 2021 16:02:49: 4000000 INFO @ Fri, 10 Dec 2021 16:02:51: 14000000 INFO @ Fri, 10 Dec 2021 16:02:53: 9000000 INFO @ Fri, 10 Dec 2021 16:02:58: 5000000 INFO @ Fri, 10 Dec 2021 16:02:59: 15000000 INFO @ Fri, 10 Dec 2021 16:03:01: 10000000 INFO @ Fri, 10 Dec 2021 16:03:07: 6000000 INFO @ Fri, 10 Dec 2021 16:03:07: 16000000 INFO @ Fri, 10 Dec 2021 16:03:09: 11000000 INFO @ Fri, 10 Dec 2021 16:03:15: 7000000 INFO @ Fri, 10 Dec 2021 16:03:16: 17000000 INFO @ Fri, 10 Dec 2021 16:03:18: 12000000 INFO @ Fri, 10 Dec 2021 16:03:24: 8000000 INFO @ Fri, 10 Dec 2021 16:03:24: 18000000 INFO @ Fri, 10 Dec 2021 16:03:26: 13000000 INFO @ Fri, 10 Dec 2021 16:03:32: 9000000 INFO @ Fri, 10 Dec 2021 16:03:32: 19000000 INFO @ Fri, 10 Dec 2021 16:03:34: 14000000 INFO @ Fri, 10 Dec 2021 16:03:40: 20000000 INFO @ Fri, 10 Dec 2021 16:03:40: 10000000 INFO @ Fri, 10 Dec 2021 16:03:42: 15000000 INFO @ Fri, 10 Dec 2021 16:03:48: 21000000 INFO @ Fri, 10 Dec 2021 16:03:49: 11000000 INFO @ Fri, 10 Dec 2021 16:03:50: 16000000 INFO @ Fri, 10 Dec 2021 16:03:56: 22000000 INFO @ Fri, 10 Dec 2021 16:03:57: 12000000 INFO @ Fri, 10 Dec 2021 16:03:58: 17000000 INFO @ Fri, 10 Dec 2021 16:04:04: 23000000 INFO @ Fri, 10 Dec 2021 16:04:05: 13000000 INFO @ Fri, 10 Dec 2021 16:04:06: 18000000 INFO @ Fri, 10 Dec 2021 16:04:12: 24000000 INFO @ Fri, 10 Dec 2021 16:04:14: 14000000 INFO @ Fri, 10 Dec 2021 16:04:14: 19000000 INFO @ Fri, 10 Dec 2021 16:04:20: 25000000 INFO @ Fri, 10 Dec 2021 16:04:22: 20000000 INFO @ Fri, 10 Dec 2021 16:04:22: 15000000 INFO @ Fri, 10 Dec 2021 16:04:28: 26000000 INFO @ Fri, 10 Dec 2021 16:04:30: 21000000 INFO @ Fri, 10 Dec 2021 16:04:31: 16000000 INFO @ Fri, 10 Dec 2021 16:04:36: 27000000 INFO @ Fri, 10 Dec 2021 16:04:38: 22000000 INFO @ Fri, 10 Dec 2021 16:04:39: 17000000 INFO @ Fri, 10 Dec 2021 16:04:44: 28000000 INFO @ Fri, 10 Dec 2021 16:04:46: 23000000 INFO @ Fri, 10 Dec 2021 16:04:48: 18000000 INFO @ Fri, 10 Dec 2021 16:04:52: 29000000 INFO @ Fri, 10 Dec 2021 16:04:54: 24000000 INFO @ Fri, 10 Dec 2021 16:04:56: 19000000 INFO @ Fri, 10 Dec 2021 16:05:00: 30000000 INFO @ Fri, 10 Dec 2021 16:05:03: 25000000 INFO @ Fri, 10 Dec 2021 16:05:04: 20000000 INFO @ Fri, 10 Dec 2021 16:05:08: 31000000 INFO @ Fri, 10 Dec 2021 16:05:11: 26000000 INFO @ Fri, 10 Dec 2021 16:05:13: 21000000 INFO @ Fri, 10 Dec 2021 16:05:16: 32000000 INFO @ Fri, 10 Dec 2021 16:05:19: 27000000 INFO @ Fri, 10 Dec 2021 16:05:22: 22000000 INFO @ Fri, 10 Dec 2021 16:05:24: 33000000 INFO @ Fri, 10 Dec 2021 16:05:27: 28000000 INFO @ Fri, 10 Dec 2021 16:05:30: 23000000 INFO @ Fri, 10 Dec 2021 16:05:32: 34000000 INFO @ Fri, 10 Dec 2021 16:05:35: 29000000 INFO @ Fri, 10 Dec 2021 16:05:39: 24000000 INFO @ Fri, 10 Dec 2021 16:05:40: 35000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 16:05:43: 30000000 INFO @ Fri, 10 Dec 2021 16:05:48: 25000000 INFO @ Fri, 10 Dec 2021 16:05:48: 36000000 INFO @ Fri, 10 Dec 2021 16:05:50: 31000000 INFO @ Fri, 10 Dec 2021 16:05:56: 26000000 INFO @ Fri, 10 Dec 2021 16:05:57: 37000000 INFO @ Fri, 10 Dec 2021 16:05:58: 32000000 INFO @ Fri, 10 Dec 2021 16:06:05: 38000000 INFO @ Fri, 10 Dec 2021 16:06:05: 27000000 INFO @ Fri, 10 Dec 2021 16:06:07: 33000000 INFO @ Fri, 10 Dec 2021 16:06:13: 39000000 INFO @ Fri, 10 Dec 2021 16:06:14: 28000000 INFO @ Fri, 10 Dec 2021 16:06:15: 34000000 INFO @ Fri, 10 Dec 2021 16:06:21: 40000000 INFO @ Fri, 10 Dec 2021 16:06:22: 29000000 INFO @ Fri, 10 Dec 2021 16:06:23: 35000000 INFO @ Fri, 10 Dec 2021 16:06:29: 41000000 INFO @ Fri, 10 Dec 2021 16:06:31: 36000000 INFO @ Fri, 10 Dec 2021 16:06:31: 30000000 INFO @ Fri, 10 Dec 2021 16:06:37: 42000000 INFO @ Fri, 10 Dec 2021 16:06:39: 37000000 INFO @ Fri, 10 Dec 2021 16:06:39: 31000000 INFO @ Fri, 10 Dec 2021 16:06:45: 43000000 INFO @ Fri, 10 Dec 2021 16:06:47: 38000000 INFO @ Fri, 10 Dec 2021 16:06:48: 32000000 INFO @ Fri, 10 Dec 2021 16:06:53: 44000000 INFO @ Fri, 10 Dec 2021 16:06:55: 39000000 INFO @ Fri, 10 Dec 2021 16:06:57: 33000000 INFO @ Fri, 10 Dec 2021 16:07:01: 45000000 INFO @ Fri, 10 Dec 2021 16:07:03: 40000000 INFO @ Fri, 10 Dec 2021 16:07:05: 34000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 16:07:09: 46000000 INFO @ Fri, 10 Dec 2021 16:07:11: 41000000 INFO @ Fri, 10 Dec 2021 16:07:14: 35000000 INFO @ Fri, 10 Dec 2021 16:07:18: 47000000 INFO @ Fri, 10 Dec 2021 16:07:19: 42000000 INFO @ Fri, 10 Dec 2021 16:07:23: 36000000 INFO @ Fri, 10 Dec 2021 16:07:26: 48000000 INFO @ Fri, 10 Dec 2021 16:07:28: 43000000 INFO @ Fri, 10 Dec 2021 16:07:31: 37000000 INFO @ Fri, 10 Dec 2021 16:07:34: 49000000 INFO @ Fri, 10 Dec 2021 16:07:35: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 16:07:35: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 16:07:35: #1 total tags in treatment: 23436294 INFO @ Fri, 10 Dec 2021 16:07:35: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 16:07:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 16:07:36: 44000000 INFO @ Fri, 10 Dec 2021 16:07:36: #1 tags after filtering in treatment: 21789441 INFO @ Fri, 10 Dec 2021 16:07:36: #1 Redundant rate of treatment: 0.07 INFO @ Fri, 10 Dec 2021 16:07:36: #1 finished! INFO @ Fri, 10 Dec 2021 16:07:36: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 16:07:36: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 16:07:37: #2 number of paired peaks: 67 WARNING @ Fri, 10 Dec 2021 16:07:37: Too few paired peaks (67) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 16:07:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7175238/SRX7175238.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175238/SRX7175238.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175238/SRX7175238.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175238/SRX7175238.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 16:07:40: 38000000 INFO @ Fri, 10 Dec 2021 16:07:44: 45000000 INFO @ Fri, 10 Dec 2021 16:07:48: 39000000 INFO @ Fri, 10 Dec 2021 16:07:52: 46000000 INFO @ Fri, 10 Dec 2021 16:07:56: 40000000 INFO @ Fri, 10 Dec 2021 16:07:59: 47000000 INFO @ Fri, 10 Dec 2021 16:08:04: 41000000 INFO @ Fri, 10 Dec 2021 16:08:07: 48000000 INFO @ Fri, 10 Dec 2021 16:08:12: 42000000 INFO @ Fri, 10 Dec 2021 16:08:15: 49000000 INFO @ Fri, 10 Dec 2021 16:08:17: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 16:08:17: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 16:08:17: #1 total tags in treatment: 23436294 INFO @ Fri, 10 Dec 2021 16:08:17: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 16:08:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 16:08:17: #1 tags after filtering in treatment: 21789441 INFO @ Fri, 10 Dec 2021 16:08:17: #1 Redundant rate of treatment: 0.07 INFO @ Fri, 10 Dec 2021 16:08:17: #1 finished! INFO @ Fri, 10 Dec 2021 16:08:17: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 16:08:17: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 16:08:19: #2 number of paired peaks: 67 WARNING @ Fri, 10 Dec 2021 16:08:19: Too few paired peaks (67) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 16:08:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7175238/SRX7175238.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175238/SRX7175238.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175238/SRX7175238.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175238/SRX7175238.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 16:08:20: 43000000 INFO @ Fri, 10 Dec 2021 16:08:28: 44000000 INFO @ Fri, 10 Dec 2021 16:08:35: 45000000 INFO @ Fri, 10 Dec 2021 16:08:43: 46000000 INFO @ Fri, 10 Dec 2021 16:08:50: 47000000 INFO @ Fri, 10 Dec 2021 16:08:58: 48000000 INFO @ Fri, 10 Dec 2021 16:09:06: 49000000 INFO @ Fri, 10 Dec 2021 16:09:07: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 16:09:07: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 16:09:07: #1 total tags in treatment: 23436294 INFO @ Fri, 10 Dec 2021 16:09:07: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 16:09:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 16:09:08: #1 tags after filtering in treatment: 21789441 INFO @ Fri, 10 Dec 2021 16:09:08: #1 Redundant rate of treatment: 0.07 INFO @ Fri, 10 Dec 2021 16:09:08: #1 finished! INFO @ Fri, 10 Dec 2021 16:09:08: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 16:09:08: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 16:09:09: #2 number of paired peaks: 67 WARNING @ Fri, 10 Dec 2021 16:09:09: Too few paired peaks (67) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 16:09:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7175238/SRX7175238.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175238/SRX7175238.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175238/SRX7175238.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175238/SRX7175238.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling