Job ID = 14168048 SRX = SRX7175236 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 26090069 spots for SRR10485676/SRR10485676.sra Written 26090069 spots for SRR10485676/SRR10485676.sra fastq に変換しました。 bowtie でマッピング中... Your job 14169008 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:20:32 26090069 reads; of these: 26090069 (100.00%) were paired; of these: 2148543 (8.24%) aligned concordantly 0 times 17394058 (66.67%) aligned concordantly exactly 1 time 6547468 (25.10%) aligned concordantly >1 times ---- 2148543 pairs aligned concordantly 0 times; of these: 410522 (19.11%) aligned discordantly 1 time ---- 1738021 pairs aligned 0 times concordantly or discordantly; of these: 3476042 mates make up the pairs; of these: 1949738 (56.09%) aligned 0 times 782157 (22.50%) aligned exactly 1 time 744147 (21.41%) aligned >1 times 96.26% overall alignment rate Time searching: 01:20:33 Overall time: 01:20:33 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 1341574 / 24119737 = 0.0556 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 16:25:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7175236/SRX7175236.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7175236/SRX7175236.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7175236/SRX7175236.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7175236/SRX7175236.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 16:25:53: #1 read tag files... INFO @ Fri, 10 Dec 2021 16:25:53: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 16:25:59: 1000000 INFO @ Fri, 10 Dec 2021 16:26:04: 2000000 INFO @ Fri, 10 Dec 2021 16:26:10: 3000000 INFO @ Fri, 10 Dec 2021 16:26:15: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 16:26:21: 5000000 INFO @ Fri, 10 Dec 2021 16:26:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7175236/SRX7175236.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7175236/SRX7175236.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7175236/SRX7175236.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7175236/SRX7175236.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 16:26:22: #1 read tag files... INFO @ Fri, 10 Dec 2021 16:26:22: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 16:26:27: 6000000 INFO @ Fri, 10 Dec 2021 16:26:28: 1000000 INFO @ Fri, 10 Dec 2021 16:26:33: 7000000 INFO @ Fri, 10 Dec 2021 16:26:34: 2000000 INFO @ Fri, 10 Dec 2021 16:26:39: 8000000 INFO @ Fri, 10 Dec 2021 16:26:40: 3000000 INFO @ Fri, 10 Dec 2021 16:26:46: 9000000 INFO @ Fri, 10 Dec 2021 16:26:47: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 16:26:52: 10000000 INFO @ Fri, 10 Dec 2021 16:26:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7175236/SRX7175236.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7175236/SRX7175236.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7175236/SRX7175236.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7175236/SRX7175236.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 16:26:52: #1 read tag files... INFO @ Fri, 10 Dec 2021 16:26:52: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 16:26:53: 5000000 INFO @ Fri, 10 Dec 2021 16:26:58: 1000000 INFO @ Fri, 10 Dec 2021 16:26:58: 11000000 INFO @ Fri, 10 Dec 2021 16:26:59: 6000000 INFO @ Fri, 10 Dec 2021 16:27:04: 2000000 INFO @ Fri, 10 Dec 2021 16:27:04: 12000000 INFO @ Fri, 10 Dec 2021 16:27:06: 7000000 INFO @ Fri, 10 Dec 2021 16:27:10: 3000000 INFO @ Fri, 10 Dec 2021 16:27:11: 13000000 INFO @ Fri, 10 Dec 2021 16:27:12: 8000000 INFO @ Fri, 10 Dec 2021 16:27:16: 4000000 INFO @ Fri, 10 Dec 2021 16:27:17: 14000000 INFO @ Fri, 10 Dec 2021 16:27:18: 9000000 INFO @ Fri, 10 Dec 2021 16:27:22: 5000000 INFO @ Fri, 10 Dec 2021 16:27:23: 15000000 INFO @ Fri, 10 Dec 2021 16:27:25: 10000000 INFO @ Fri, 10 Dec 2021 16:27:28: 6000000 INFO @ Fri, 10 Dec 2021 16:27:30: 16000000 INFO @ Fri, 10 Dec 2021 16:27:31: 11000000 INFO @ Fri, 10 Dec 2021 16:27:34: 7000000 INFO @ Fri, 10 Dec 2021 16:27:36: 17000000 INFO @ Fri, 10 Dec 2021 16:27:37: 12000000 INFO @ Fri, 10 Dec 2021 16:27:40: 8000000 INFO @ Fri, 10 Dec 2021 16:27:43: 18000000 INFO @ Fri, 10 Dec 2021 16:27:44: 13000000 INFO @ Fri, 10 Dec 2021 16:27:45: 9000000 INFO @ Fri, 10 Dec 2021 16:27:49: 19000000 INFO @ Fri, 10 Dec 2021 16:27:50: 14000000 INFO @ Fri, 10 Dec 2021 16:27:51: 10000000 INFO @ Fri, 10 Dec 2021 16:27:55: 20000000 INFO @ Fri, 10 Dec 2021 16:27:56: 15000000 INFO @ Fri, 10 Dec 2021 16:27:57: 11000000 INFO @ Fri, 10 Dec 2021 16:28:02: 21000000 INFO @ Fri, 10 Dec 2021 16:28:03: 16000000 INFO @ Fri, 10 Dec 2021 16:28:03: 12000000 INFO @ Fri, 10 Dec 2021 16:28:08: 22000000 INFO @ Fri, 10 Dec 2021 16:28:09: 13000000 INFO @ Fri, 10 Dec 2021 16:28:09: 17000000 INFO @ Fri, 10 Dec 2021 16:28:14: 23000000 INFO @ Fri, 10 Dec 2021 16:28:15: 14000000 INFO @ Fri, 10 Dec 2021 16:28:15: 18000000 INFO @ Fri, 10 Dec 2021 16:28:21: 24000000 INFO @ Fri, 10 Dec 2021 16:28:21: 15000000 INFO @ Fri, 10 Dec 2021 16:28:22: 19000000 INFO @ Fri, 10 Dec 2021 16:28:27: 25000000 INFO @ Fri, 10 Dec 2021 16:28:27: 16000000 INFO @ Fri, 10 Dec 2021 16:28:28: 20000000 INFO @ Fri, 10 Dec 2021 16:28:33: 17000000 INFO @ Fri, 10 Dec 2021 16:28:33: 26000000 INFO @ Fri, 10 Dec 2021 16:28:34: 21000000 INFO @ Fri, 10 Dec 2021 16:28:39: 18000000 INFO @ Fri, 10 Dec 2021 16:28:39: 27000000 INFO @ Fri, 10 Dec 2021 16:28:40: 22000000 INFO @ Fri, 10 Dec 2021 16:28:45: 19000000 INFO @ Fri, 10 Dec 2021 16:28:46: 28000000 INFO @ Fri, 10 Dec 2021 16:28:47: 23000000 INFO @ Fri, 10 Dec 2021 16:28:51: 20000000 INFO @ Fri, 10 Dec 2021 16:28:52: 29000000 INFO @ Fri, 10 Dec 2021 16:28:53: 24000000 INFO @ Fri, 10 Dec 2021 16:28:57: 21000000 INFO @ Fri, 10 Dec 2021 16:28:58: 30000000 INFO @ Fri, 10 Dec 2021 16:28:59: 25000000 INFO @ Fri, 10 Dec 2021 16:29:02: 22000000 INFO @ Fri, 10 Dec 2021 16:29:04: 31000000 INFO @ Fri, 10 Dec 2021 16:29:06: 26000000 INFO @ Fri, 10 Dec 2021 16:29:08: 23000000 INFO @ Fri, 10 Dec 2021 16:29:11: 32000000 INFO @ Fri, 10 Dec 2021 16:29:12: 27000000 INFO @ Fri, 10 Dec 2021 16:29:14: 24000000 INFO @ Fri, 10 Dec 2021 16:29:17: 33000000 INFO @ Fri, 10 Dec 2021 16:29:18: 28000000 INFO @ Fri, 10 Dec 2021 16:29:20: 25000000 INFO @ Fri, 10 Dec 2021 16:29:23: 34000000 INFO @ Fri, 10 Dec 2021 16:29:24: 29000000 INFO @ Fri, 10 Dec 2021 16:29:26: 26000000 INFO @ Fri, 10 Dec 2021 16:29:30: 35000000 INFO @ Fri, 10 Dec 2021 16:29:31: 30000000 INFO @ Fri, 10 Dec 2021 16:29:31: 27000000 INFO @ Fri, 10 Dec 2021 16:29:36: 36000000 INFO @ Fri, 10 Dec 2021 16:29:37: 31000000 INFO @ Fri, 10 Dec 2021 16:29:37: 28000000 INFO @ Fri, 10 Dec 2021 16:29:42: 37000000 INFO @ Fri, 10 Dec 2021 16:29:43: 32000000 INFO @ Fri, 10 Dec 2021 16:29:43: 29000000 INFO @ Fri, 10 Dec 2021 16:29:48: 38000000 INFO @ Fri, 10 Dec 2021 16:29:49: 33000000 INFO @ Fri, 10 Dec 2021 16:29:49: 30000000 INFO @ Fri, 10 Dec 2021 16:29:54: 39000000 INFO @ Fri, 10 Dec 2021 16:29:55: 31000000 INFO @ Fri, 10 Dec 2021 16:29:55: 34000000 INFO @ Fri, 10 Dec 2021 16:30:01: 40000000 INFO @ Fri, 10 Dec 2021 16:30:01: 32000000 INFO @ Fri, 10 Dec 2021 16:30:01: 35000000 INFO @ Fri, 10 Dec 2021 16:30:07: 33000000 INFO @ Fri, 10 Dec 2021 16:30:07: 41000000 INFO @ Fri, 10 Dec 2021 16:30:08: 36000000 INFO @ Fri, 10 Dec 2021 16:30:13: 34000000 INFO @ Fri, 10 Dec 2021 16:30:13: 42000000 INFO @ Fri, 10 Dec 2021 16:30:14: 37000000 INFO @ Fri, 10 Dec 2021 16:30:18: 35000000 INFO @ Fri, 10 Dec 2021 16:30:20: 43000000 INFO @ Fri, 10 Dec 2021 16:30:20: 38000000 INFO @ Fri, 10 Dec 2021 16:30:24: 36000000 INFO @ Fri, 10 Dec 2021 16:30:26: 44000000 INFO @ Fri, 10 Dec 2021 16:30:26: 39000000 INFO @ Fri, 10 Dec 2021 16:30:30: 37000000 INFO @ Fri, 10 Dec 2021 16:30:32: 45000000 INFO @ Fri, 10 Dec 2021 16:30:32: 40000000 INFO @ Fri, 10 Dec 2021 16:30:36: 38000000 INFO @ Fri, 10 Dec 2021 16:30:38: 46000000 INFO @ Fri, 10 Dec 2021 16:30:39: 41000000 INFO @ Fri, 10 Dec 2021 16:30:42: 39000000 INFO @ Fri, 10 Dec 2021 16:30:44: 47000000 INFO @ Fri, 10 Dec 2021 16:30:45: 42000000 INFO @ Fri, 10 Dec 2021 16:30:48: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 16:30:48: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 16:30:48: #1 total tags in treatment: 22605262 INFO @ Fri, 10 Dec 2021 16:30:48: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 16:30:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 16:30:48: 40000000 INFO @ Fri, 10 Dec 2021 16:30:48: #1 tags after filtering in treatment: 21009669 INFO @ Fri, 10 Dec 2021 16:30:48: #1 Redundant rate of treatment: 0.07 INFO @ Fri, 10 Dec 2021 16:30:48: #1 finished! INFO @ Fri, 10 Dec 2021 16:30:48: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 16:30:48: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 16:30:50: #2 number of paired peaks: 65 WARNING @ Fri, 10 Dec 2021 16:30:50: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 16:30:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7175236/SRX7175236.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175236/SRX7175236.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175236/SRX7175236.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175236/SRX7175236.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 16:30:51: 43000000 INFO @ Fri, 10 Dec 2021 16:30:54: 41000000 INFO @ Fri, 10 Dec 2021 16:30:57: 44000000 INFO @ Fri, 10 Dec 2021 16:31:00: 42000000 INFO @ Fri, 10 Dec 2021 16:31:02: 45000000 INFO @ Fri, 10 Dec 2021 16:31:06: 43000000 INFO @ Fri, 10 Dec 2021 16:31:08: 46000000 INFO @ Fri, 10 Dec 2021 16:31:12: 44000000 INFO @ Fri, 10 Dec 2021 16:31:13: 47000000 INFO @ Fri, 10 Dec 2021 16:31:17: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 16:31:17: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 16:31:17: #1 total tags in treatment: 22605262 INFO @ Fri, 10 Dec 2021 16:31:17: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 16:31:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 16:31:17: #1 tags after filtering in treatment: 21009669 INFO @ Fri, 10 Dec 2021 16:31:17: #1 Redundant rate of treatment: 0.07 INFO @ Fri, 10 Dec 2021 16:31:17: #1 finished! INFO @ Fri, 10 Dec 2021 16:31:17: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 16:31:17: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 16:31:18: 45000000 INFO @ Fri, 10 Dec 2021 16:31:19: #2 number of paired peaks: 65 WARNING @ Fri, 10 Dec 2021 16:31:19: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 16:31:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7175236/SRX7175236.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175236/SRX7175236.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175236/SRX7175236.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175236/SRX7175236.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 16:31:23: 46000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 16:31:29: 47000000 INFO @ Fri, 10 Dec 2021 16:31:32: #1 tag size is determined as 75 bps INFO @ Fri, 10 Dec 2021 16:31:32: #1 tag size = 75 INFO @ Fri, 10 Dec 2021 16:31:32: #1 total tags in treatment: 22605262 INFO @ Fri, 10 Dec 2021 16:31:32: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 16:31:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 16:31:33: #1 tags after filtering in treatment: 21009669 INFO @ Fri, 10 Dec 2021 16:31:33: #1 Redundant rate of treatment: 0.07 INFO @ Fri, 10 Dec 2021 16:31:33: #1 finished! INFO @ Fri, 10 Dec 2021 16:31:33: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 16:31:33: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 16:31:34: #2 number of paired peaks: 65 WARNING @ Fri, 10 Dec 2021 16:31:34: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 10 Dec 2021 16:31:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7175236/SRX7175236.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175236/SRX7175236.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175236/SRX7175236.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7175236/SRX7175236.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。