Job ID = 6528476 SRX = SRX7158311 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T15:27:06 prefetch.2.10.7: 1) Downloading 'SRR10467037'... 2020-06-29T15:27:06 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:33:21 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:33:21 prefetch.2.10.7: 1) 'SRR10467037' was downloaded successfully 2020-06-29T15:33:21 prefetch.2.10.7: 'SRR10467037' has 0 unresolved dependencies Read 45292235 spots for SRR10467037/SRR10467037.sra Written 45292235 spots for SRR10467037/SRR10467037.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:19:44 45292235 reads; of these: 45292235 (100.00%) were unpaired; of these: 1590441 (3.51%) aligned 0 times 34959660 (77.19%) aligned exactly 1 time 8742134 (19.30%) aligned >1 times 96.49% overall alignment rate Time searching: 00:19:44 Overall time: 00:19:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 5745193 / 43701794 = 0.1315 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:26:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7158311/SRX7158311.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7158311/SRX7158311.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7158311/SRX7158311.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7158311/SRX7158311.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:26:44: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:26:44: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:26:49: 1000000 INFO @ Tue, 30 Jun 2020 01:26:55: 2000000 INFO @ Tue, 30 Jun 2020 01:27:01: 3000000 INFO @ Tue, 30 Jun 2020 01:27:07: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:27:12: 5000000 INFO @ Tue, 30 Jun 2020 01:27:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7158311/SRX7158311.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7158311/SRX7158311.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7158311/SRX7158311.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7158311/SRX7158311.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:27:13: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:27:13: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:27:18: 6000000 INFO @ Tue, 30 Jun 2020 01:27:20: 1000000 INFO @ Tue, 30 Jun 2020 01:27:24: 7000000 INFO @ Tue, 30 Jun 2020 01:27:26: 2000000 INFO @ Tue, 30 Jun 2020 01:27:31: 8000000 INFO @ Tue, 30 Jun 2020 01:27:32: 3000000 INFO @ Tue, 30 Jun 2020 01:27:37: 9000000 INFO @ Tue, 30 Jun 2020 01:27:38: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:27:43: 10000000 INFO @ Tue, 30 Jun 2020 01:27:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7158311/SRX7158311.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7158311/SRX7158311.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7158311/SRX7158311.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7158311/SRX7158311.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:27:43: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:27:43: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:27:45: 5000000 INFO @ Tue, 30 Jun 2020 01:27:49: 11000000 INFO @ Tue, 30 Jun 2020 01:27:50: 1000000 INFO @ Tue, 30 Jun 2020 01:27:51: 6000000 INFO @ Tue, 30 Jun 2020 01:27:56: 12000000 INFO @ Tue, 30 Jun 2020 01:27:56: 2000000 INFO @ Tue, 30 Jun 2020 01:27:57: 7000000 INFO @ Tue, 30 Jun 2020 01:28:02: 13000000 INFO @ Tue, 30 Jun 2020 01:28:02: 3000000 INFO @ Tue, 30 Jun 2020 01:28:04: 8000000 INFO @ Tue, 30 Jun 2020 01:28:08: 14000000 INFO @ Tue, 30 Jun 2020 01:28:08: 4000000 INFO @ Tue, 30 Jun 2020 01:28:10: 9000000 INFO @ Tue, 30 Jun 2020 01:28:14: 15000000 INFO @ Tue, 30 Jun 2020 01:28:15: 5000000 INFO @ Tue, 30 Jun 2020 01:28:16: 10000000 INFO @ Tue, 30 Jun 2020 01:28:21: 16000000 INFO @ Tue, 30 Jun 2020 01:28:21: 6000000 INFO @ Tue, 30 Jun 2020 01:28:23: 11000000 INFO @ Tue, 30 Jun 2020 01:28:27: 17000000 INFO @ Tue, 30 Jun 2020 01:28:27: 7000000 INFO @ Tue, 30 Jun 2020 01:28:29: 12000000 INFO @ Tue, 30 Jun 2020 01:28:33: 18000000 INFO @ Tue, 30 Jun 2020 01:28:34: 8000000 INFO @ Tue, 30 Jun 2020 01:28:35: 13000000 INFO @ Tue, 30 Jun 2020 01:28:40: 19000000 INFO @ Tue, 30 Jun 2020 01:28:40: 9000000 INFO @ Tue, 30 Jun 2020 01:28:42: 14000000 INFO @ Tue, 30 Jun 2020 01:28:46: 20000000 INFO @ Tue, 30 Jun 2020 01:28:46: 10000000 INFO @ Tue, 30 Jun 2020 01:28:48: 15000000 INFO @ Tue, 30 Jun 2020 01:28:52: 21000000 INFO @ Tue, 30 Jun 2020 01:28:52: 11000000 INFO @ Tue, 30 Jun 2020 01:28:54: 16000000 INFO @ Tue, 30 Jun 2020 01:28:58: 22000000 INFO @ Tue, 30 Jun 2020 01:28:59: 12000000 INFO @ Tue, 30 Jun 2020 01:29:01: 17000000 INFO @ Tue, 30 Jun 2020 01:29:05: 23000000 INFO @ Tue, 30 Jun 2020 01:29:05: 13000000 INFO @ Tue, 30 Jun 2020 01:29:07: 18000000 INFO @ Tue, 30 Jun 2020 01:29:11: 24000000 INFO @ Tue, 30 Jun 2020 01:29:11: 14000000 INFO @ Tue, 30 Jun 2020 01:29:13: 19000000 INFO @ Tue, 30 Jun 2020 01:29:17: 25000000 INFO @ Tue, 30 Jun 2020 01:29:17: 15000000 INFO @ Tue, 30 Jun 2020 01:29:20: 20000000 INFO @ Tue, 30 Jun 2020 01:29:24: 26000000 INFO @ Tue, 30 Jun 2020 01:29:24: 16000000 INFO @ Tue, 30 Jun 2020 01:29:26: 21000000 INFO @ Tue, 30 Jun 2020 01:29:30: 17000000 INFO @ Tue, 30 Jun 2020 01:29:30: 27000000 INFO @ Tue, 30 Jun 2020 01:29:33: 22000000 INFO @ Tue, 30 Jun 2020 01:29:36: 28000000 INFO @ Tue, 30 Jun 2020 01:29:37: 18000000 INFO @ Tue, 30 Jun 2020 01:29:39: 23000000 INFO @ Tue, 30 Jun 2020 01:29:43: 19000000 INFO @ Tue, 30 Jun 2020 01:29:43: 29000000 INFO @ Tue, 30 Jun 2020 01:29:45: 24000000 INFO @ Tue, 30 Jun 2020 01:29:49: 20000000 INFO @ Tue, 30 Jun 2020 01:29:49: 30000000 INFO @ Tue, 30 Jun 2020 01:29:52: 25000000 INFO @ Tue, 30 Jun 2020 01:29:55: 21000000 INFO @ Tue, 30 Jun 2020 01:29:55: 31000000 INFO @ Tue, 30 Jun 2020 01:29:58: 26000000 INFO @ Tue, 30 Jun 2020 01:30:02: 22000000 INFO @ Tue, 30 Jun 2020 01:30:02: 32000000 INFO @ Tue, 30 Jun 2020 01:30:05: 27000000 INFO @ Tue, 30 Jun 2020 01:30:08: 23000000 INFO @ Tue, 30 Jun 2020 01:30:08: 33000000 INFO @ Tue, 30 Jun 2020 01:30:11: 28000000 INFO @ Tue, 30 Jun 2020 01:30:14: 24000000 INFO @ Tue, 30 Jun 2020 01:30:15: 34000000 INFO @ Tue, 30 Jun 2020 01:30:18: 29000000 INFO @ Tue, 30 Jun 2020 01:30:21: 25000000 INFO @ Tue, 30 Jun 2020 01:30:21: 35000000 INFO @ Tue, 30 Jun 2020 01:30:24: 30000000 INFO @ Tue, 30 Jun 2020 01:30:27: 26000000 INFO @ Tue, 30 Jun 2020 01:30:27: 36000000 INFO @ Tue, 30 Jun 2020 01:30:31: 31000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 01:30:33: 27000000 INFO @ Tue, 30 Jun 2020 01:30:34: 37000000 INFO @ Tue, 30 Jun 2020 01:30:37: 32000000 INFO @ Tue, 30 Jun 2020 01:30:40: 28000000 INFO @ Tue, 30 Jun 2020 01:30:40: #1 tag size is determined as 75 bps INFO @ Tue, 30 Jun 2020 01:30:40: #1 tag size = 75 INFO @ Tue, 30 Jun 2020 01:30:40: #1 total tags in treatment: 37956601 INFO @ Tue, 30 Jun 2020 01:30:40: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:30:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:30:40: #1 tags after filtering in treatment: 37956601 INFO @ Tue, 30 Jun 2020 01:30:40: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:30:40: #1 finished! INFO @ Tue, 30 Jun 2020 01:30:40: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:30:40: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:30:43: #2 number of paired peaks: 49 WARNING @ Tue, 30 Jun 2020 01:30:43: Too few paired peaks (49) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:30:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7158311/SRX7158311.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7158311/SRX7158311.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7158311/SRX7158311.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7158311/SRX7158311.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 01:30:44: 33000000 INFO @ Tue, 30 Jun 2020 01:30:46: 29000000 INFO @ Tue, 30 Jun 2020 01:30:50: 34000000 INFO @ Tue, 30 Jun 2020 01:30:52: 30000000 INFO @ Tue, 30 Jun 2020 01:30:57: 35000000 INFO @ Tue, 30 Jun 2020 01:30:59: 31000000 INFO @ Tue, 30 Jun 2020 01:31:03: 36000000 INFO @ Tue, 30 Jun 2020 01:31:05: 32000000 INFO @ Tue, 30 Jun 2020 01:31:10: 37000000 INFO @ Tue, 30 Jun 2020 01:31:11: 33000000 INFO @ Tue, 30 Jun 2020 01:31:16: #1 tag size is determined as 75 bps INFO @ Tue, 30 Jun 2020 01:31:16: #1 tag size = 75 INFO @ Tue, 30 Jun 2020 01:31:16: #1 total tags in treatment: 37956601 INFO @ Tue, 30 Jun 2020 01:31:16: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:31:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:31:16: #1 tags after filtering in treatment: 37956601 INFO @ Tue, 30 Jun 2020 01:31:16: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:31:16: #1 finished! INFO @ Tue, 30 Jun 2020 01:31:16: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:31:16: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:31:17: 34000000 INFO @ Tue, 30 Jun 2020 01:31:19: #2 number of paired peaks: 49 WARNING @ Tue, 30 Jun 2020 01:31:19: Too few paired peaks (49) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:31:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7158311/SRX7158311.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7158311/SRX7158311.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7158311/SRX7158311.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7158311/SRX7158311.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 01:31:23: 35000000 INFO @ Tue, 30 Jun 2020 01:31:29: 36000000 INFO @ Tue, 30 Jun 2020 01:31:35: 37000000 INFO @ Tue, 30 Jun 2020 01:31:41: #1 tag size is determined as 75 bps INFO @ Tue, 30 Jun 2020 01:31:41: #1 tag size = 75 INFO @ Tue, 30 Jun 2020 01:31:41: #1 total tags in treatment: 37956601 INFO @ Tue, 30 Jun 2020 01:31:41: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:31:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:31:41: #1 tags after filtering in treatment: 37956601 INFO @ Tue, 30 Jun 2020 01:31:41: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:31:41: #1 finished! INFO @ Tue, 30 Jun 2020 01:31:41: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:31:41: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:31:44: #2 number of paired peaks: 49 WARNING @ Tue, 30 Jun 2020 01:31:44: Too few paired peaks (49) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:31:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7158311/SRX7158311.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7158311/SRX7158311.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7158311/SRX7158311.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7158311/SRX7158311.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。