Job ID = 4178650 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 74,659,995 reads read : 149,319,990 reads written : 74,659,995 reads 0-length : 74,659,995 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:48:05 74659995 reads; of these: 74659995 (100.00%) were unpaired; of these: 3477546 (4.66%) aligned 0 times 46874054 (62.78%) aligned exactly 1 time 24308395 (32.56%) aligned >1 times 95.34% overall alignment rate Time searching: 00:48:06 Overall time: 00:48:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 16052135 / 71182449 = 0.2255 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Thu, 05 Dec 2019 15:18:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7158308/SRX7158308.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7158308/SRX7158308.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7158308/SRX7158308.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7158308/SRX7158308.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Dec 2019 15:18:08: #1 read tag files... INFO @ Thu, 05 Dec 2019 15:18:08: #1 read treatment tags... INFO @ Thu, 05 Dec 2019 15:18:17: 1000000 INFO @ Thu, 05 Dec 2019 15:18:25: 2000000 INFO @ Thu, 05 Dec 2019 15:18:34: 3000000 INFO @ Thu, 05 Dec 2019 15:18:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7158308/SRX7158308.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7158308/SRX7158308.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7158308/SRX7158308.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7158308/SRX7158308.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Dec 2019 15:18:37: #1 read tag files... INFO @ Thu, 05 Dec 2019 15:18:37: #1 read treatment tags... INFO @ Thu, 05 Dec 2019 15:18:42: 4000000 INFO @ Thu, 05 Dec 2019 15:18:46: 1000000 INFO @ Thu, 05 Dec 2019 15:18:52: 5000000 INFO @ Thu, 05 Dec 2019 15:18:55: 2000000 INFO @ Thu, 05 Dec 2019 15:19:01: 6000000 INFO @ Thu, 05 Dec 2019 15:19:03: 3000000 BedGraph に変換中... INFO @ Thu, 05 Dec 2019 15:19:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX7158308/SRX7158308.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX7158308/SRX7158308.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX7158308/SRX7158308.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX7158308/SRX7158308.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Dec 2019 15:19:07: #1 read tag files... INFO @ Thu, 05 Dec 2019 15:19:07: #1 read treatment tags... INFO @ Thu, 05 Dec 2019 15:19:09: 7000000 INFO @ Thu, 05 Dec 2019 15:19:12: 4000000 INFO @ Thu, 05 Dec 2019 15:19:17: 1000000 INFO @ Thu, 05 Dec 2019 15:19:17: 8000000 INFO @ Thu, 05 Dec 2019 15:19:21: 5000000 INFO @ Thu, 05 Dec 2019 15:19:25: 9000000 INFO @ Thu, 05 Dec 2019 15:19:26: 2000000 INFO @ Thu, 05 Dec 2019 15:19:30: 6000000 INFO @ Thu, 05 Dec 2019 15:19:33: 10000000 INFO @ Thu, 05 Dec 2019 15:19:35: 3000000 INFO @ Thu, 05 Dec 2019 15:19:39: 7000000 INFO @ Thu, 05 Dec 2019 15:19:41: 11000000 INFO @ Thu, 05 Dec 2019 15:19:44: 4000000 INFO @ Thu, 05 Dec 2019 15:19:47: 8000000 INFO @ Thu, 05 Dec 2019 15:19:49: 12000000 INFO @ Thu, 05 Dec 2019 15:19:53: 5000000 INFO @ Thu, 05 Dec 2019 15:19:56: 9000000 INFO @ Thu, 05 Dec 2019 15:19:57: 13000000 INFO @ Thu, 05 Dec 2019 15:20:01: 6000000 INFO @ Thu, 05 Dec 2019 15:20:04: 14000000 INFO @ Thu, 05 Dec 2019 15:20:05: 10000000 INFO @ Thu, 05 Dec 2019 15:20:10: 7000000 INFO @ Thu, 05 Dec 2019 15:20:12: 15000000 INFO @ Thu, 05 Dec 2019 15:20:14: 11000000 INFO @ Thu, 05 Dec 2019 15:20:19: 8000000 INFO @ Thu, 05 Dec 2019 15:20:20: 16000000 INFO @ Thu, 05 Dec 2019 15:20:22: 12000000 INFO @ Thu, 05 Dec 2019 15:20:28: 9000000 INFO @ Thu, 05 Dec 2019 15:20:28: 17000000 INFO @ Thu, 05 Dec 2019 15:20:31: 13000000 INFO @ Thu, 05 Dec 2019 15:20:35: 18000000 INFO @ Thu, 05 Dec 2019 15:20:36: 10000000 INFO @ Thu, 05 Dec 2019 15:20:40: 14000000 INFO @ Thu, 05 Dec 2019 15:20:43: 19000000 INFO @ Thu, 05 Dec 2019 15:20:45: 11000000 INFO @ Thu, 05 Dec 2019 15:20:49: 15000000 INFO @ Thu, 05 Dec 2019 15:20:51: 20000000 INFO @ Thu, 05 Dec 2019 15:20:53: 12000000 INFO @ Thu, 05 Dec 2019 15:20:57: 16000000 INFO @ Thu, 05 Dec 2019 15:20:59: 21000000 INFO @ Thu, 05 Dec 2019 15:21:02: 13000000 INFO @ Thu, 05 Dec 2019 15:21:06: 17000000 INFO @ Thu, 05 Dec 2019 15:21:07: 22000000 INFO @ Thu, 05 Dec 2019 15:21:10: 14000000 INFO @ Thu, 05 Dec 2019 15:21:15: 18000000 INFO @ Thu, 05 Dec 2019 15:21:15: 23000000 INFO @ Thu, 05 Dec 2019 15:21:19: 15000000 INFO @ Thu, 05 Dec 2019 15:21:22: 24000000 INFO @ Thu, 05 Dec 2019 15:21:23: 19000000 INFO @ Thu, 05 Dec 2019 15:21:28: 16000000 INFO @ Thu, 05 Dec 2019 15:21:31: 25000000 INFO @ Thu, 05 Dec 2019 15:21:31: 20000000 INFO @ Thu, 05 Dec 2019 15:21:36: 17000000 INFO @ Thu, 05 Dec 2019 15:21:38: 26000000 INFO @ Thu, 05 Dec 2019 15:21:40: 21000000 INFO @ Thu, 05 Dec 2019 15:21:45: 18000000 INFO @ Thu, 05 Dec 2019 15:21:46: 27000000 INFO @ Thu, 05 Dec 2019 15:21:48: 22000000 INFO @ Thu, 05 Dec 2019 15:21:53: 19000000 INFO @ Thu, 05 Dec 2019 15:21:53: 28000000 INFO @ Thu, 05 Dec 2019 15:21:57: 23000000 INFO @ Thu, 05 Dec 2019 15:22:01: 29000000 INFO @ Thu, 05 Dec 2019 15:22:02: 20000000 INFO @ Thu, 05 Dec 2019 15:22:05: 24000000 INFO @ Thu, 05 Dec 2019 15:22:09: 30000000 INFO @ Thu, 05 Dec 2019 15:22:10: 21000000 INFO @ Thu, 05 Dec 2019 15:22:13: 25000000 INFO @ Thu, 05 Dec 2019 15:22:18: 31000000 INFO @ Thu, 05 Dec 2019 15:22:18: 22000000 INFO @ Thu, 05 Dec 2019 15:22:22: 26000000 INFO @ Thu, 05 Dec 2019 15:22:26: 32000000 INFO @ Thu, 05 Dec 2019 15:22:27: 23000000 INFO @ Thu, 05 Dec 2019 15:22:30: 27000000 INFO @ Thu, 05 Dec 2019 15:22:34: 33000000 INFO @ Thu, 05 Dec 2019 15:22:35: 24000000 INFO @ Thu, 05 Dec 2019 15:22:38: 28000000 INFO @ Thu, 05 Dec 2019 15:22:43: 34000000 INFO @ Thu, 05 Dec 2019 15:22:44: 25000000 INFO @ Thu, 05 Dec 2019 15:22:47: 29000000 INFO @ Thu, 05 Dec 2019 15:22:52: 35000000 INFO @ Thu, 05 Dec 2019 15:22:52: 26000000 INFO @ Thu, 05 Dec 2019 15:22:55: 30000000 INFO @ Thu, 05 Dec 2019 15:23:01: 36000000 INFO @ Thu, 05 Dec 2019 15:23:01: 27000000 INFO @ Thu, 05 Dec 2019 15:23:04: 31000000 INFO @ Thu, 05 Dec 2019 15:23:09: 28000000 INFO @ Thu, 05 Dec 2019 15:23:10: 37000000 INFO @ Thu, 05 Dec 2019 15:23:12: 32000000 INFO @ Thu, 05 Dec 2019 15:23:18: 29000000 INFO @ Thu, 05 Dec 2019 15:23:19: 38000000 INFO @ Thu, 05 Dec 2019 15:23:20: 33000000 INFO @ Thu, 05 Dec 2019 15:23:26: 30000000 INFO @ Thu, 05 Dec 2019 15:23:28: 39000000 INFO @ Thu, 05 Dec 2019 15:23:30: 34000000 INFO @ Thu, 05 Dec 2019 15:23:34: 31000000 INFO @ Thu, 05 Dec 2019 15:23:38: 40000000 INFO @ Thu, 05 Dec 2019 15:23:39: 35000000 INFO @ Thu, 05 Dec 2019 15:23:43: 32000000 INFO @ Thu, 05 Dec 2019 15:23:48: 41000000 INFO @ Thu, 05 Dec 2019 15:23:48: 36000000 INFO @ Thu, 05 Dec 2019 15:23:51: 33000000 INFO @ Thu, 05 Dec 2019 15:23:56: 37000000 INFO @ Thu, 05 Dec 2019 15:23:57: 42000000 INFO @ Thu, 05 Dec 2019 15:24:00: 34000000 INFO @ Thu, 05 Dec 2019 15:24:05: 38000000 INFO @ Thu, 05 Dec 2019 15:24:05: 43000000 INFO @ Thu, 05 Dec 2019 15:24:09: 35000000 INFO @ Thu, 05 Dec 2019 15:24:14: 39000000 INFO @ Thu, 05 Dec 2019 15:24:15: 44000000 INFO @ Thu, 05 Dec 2019 15:24:18: 36000000 INFO @ Thu, 05 Dec 2019 15:24:23: 40000000 INFO @ Thu, 05 Dec 2019 15:24:24: 45000000 INFO @ Thu, 05 Dec 2019 15:24:27: 37000000 INFO @ Thu, 05 Dec 2019 15:24:32: 41000000 INFO @ Thu, 05 Dec 2019 15:24:34: 46000000 INFO @ Thu, 05 Dec 2019 15:24:36: 38000000 INFO @ Thu, 05 Dec 2019 15:24:40: 42000000 INFO @ Thu, 05 Dec 2019 15:24:43: 47000000 INFO @ Thu, 05 Dec 2019 15:24:44: 39000000 INFO @ Thu, 05 Dec 2019 15:24:49: 43000000 INFO @ Thu, 05 Dec 2019 15:24:51: 48000000 INFO @ Thu, 05 Dec 2019 15:24:53: 40000000 INFO @ Thu, 05 Dec 2019 15:24:57: 44000000 INFO @ Thu, 05 Dec 2019 15:25:00: 49000000 INFO @ Thu, 05 Dec 2019 15:25:02: 41000000 INFO @ Thu, 05 Dec 2019 15:25:06: 45000000 INFO @ Thu, 05 Dec 2019 15:25:09: 50000000 INFO @ Thu, 05 Dec 2019 15:25:10: 42000000 INFO @ Thu, 05 Dec 2019 15:25:14: 46000000 INFO @ Thu, 05 Dec 2019 15:25:18: 51000000 INFO @ Thu, 05 Dec 2019 15:25:19: 43000000 INFO @ Thu, 05 Dec 2019 15:25:23: 47000000 INFO @ Thu, 05 Dec 2019 15:25:27: 52000000 INFO @ Thu, 05 Dec 2019 15:25:28: 44000000 INFO @ Thu, 05 Dec 2019 15:25:31: 48000000 INFO @ Thu, 05 Dec 2019 15:25:36: 53000000 INFO @ Thu, 05 Dec 2019 15:25:36: 45000000 INFO @ Thu, 05 Dec 2019 15:25:39: 49000000 INFO @ Thu, 05 Dec 2019 15:25:45: 46000000 INFO @ Thu, 05 Dec 2019 15:25:45: 54000000 INFO @ Thu, 05 Dec 2019 15:25:48: 50000000 INFO @ Thu, 05 Dec 2019 15:25:54: 47000000 INFO @ Thu, 05 Dec 2019 15:25:54: 55000000 INFO @ Thu, 05 Dec 2019 15:25:55: #1 tag size is determined as 75 bps INFO @ Thu, 05 Dec 2019 15:25:55: #1 tag size = 75 INFO @ Thu, 05 Dec 2019 15:25:55: #1 total tags in treatment: 55130314 INFO @ Thu, 05 Dec 2019 15:25:55: #1 user defined the maximum tags... INFO @ Thu, 05 Dec 2019 15:25:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Dec 2019 15:25:56: 51000000 INFO @ Thu, 05 Dec 2019 15:25:56: #1 tags after filtering in treatment: 55130314 INFO @ Thu, 05 Dec 2019 15:25:56: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Dec 2019 15:25:56: #1 finished! INFO @ Thu, 05 Dec 2019 15:25:56: #2 Build Peak Model... INFO @ Thu, 05 Dec 2019 15:25:56: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Dec 2019 15:26:00: #2 number of paired peaks: 0 WARNING @ Thu, 05 Dec 2019 15:26:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Dec 2019 15:26:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7158308/SRX7158308.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7158308/SRX7158308.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7158308/SRX7158308.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7158308/SRX7158308.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 05 Dec 2019 15:26:02: 48000000 INFO @ Thu, 05 Dec 2019 15:26:04: 52000000 INFO @ Thu, 05 Dec 2019 15:26:10: 49000000 INFO @ Thu, 05 Dec 2019 15:26:13: 53000000 INFO @ Thu, 05 Dec 2019 15:26:19: 50000000 INFO @ Thu, 05 Dec 2019 15:26:21: 54000000 INFO @ Thu, 05 Dec 2019 15:26:27: 51000000 INFO @ Thu, 05 Dec 2019 15:26:29: 55000000 INFO @ Thu, 05 Dec 2019 15:26:30: #1 tag size is determined as 75 bps INFO @ Thu, 05 Dec 2019 15:26:30: #1 tag size = 75 INFO @ Thu, 05 Dec 2019 15:26:30: #1 total tags in treatment: 55130314 INFO @ Thu, 05 Dec 2019 15:26:30: #1 user defined the maximum tags... INFO @ Thu, 05 Dec 2019 15:26:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Dec 2019 15:26:31: #1 tags after filtering in treatment: 55130314 INFO @ Thu, 05 Dec 2019 15:26:31: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Dec 2019 15:26:31: #1 finished! INFO @ Thu, 05 Dec 2019 15:26:31: #2 Build Peak Model... INFO @ Thu, 05 Dec 2019 15:26:31: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Dec 2019 15:26:35: #2 number of paired peaks: 0 WARNING @ Thu, 05 Dec 2019 15:26:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Dec 2019 15:26:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7158308/SRX7158308.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7158308/SRX7158308.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7158308/SRX7158308.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7158308/SRX7158308.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 05 Dec 2019 15:26:35: 52000000 INFO @ Thu, 05 Dec 2019 15:26:43: 53000000 INFO @ Thu, 05 Dec 2019 15:26:51: 54000000 INFO @ Thu, 05 Dec 2019 15:26:59: 55000000 INFO @ Thu, 05 Dec 2019 15:27:01: #1 tag size is determined as 75 bps INFO @ Thu, 05 Dec 2019 15:27:01: #1 tag size = 75 INFO @ Thu, 05 Dec 2019 15:27:01: #1 total tags in treatment: 55130314 INFO @ Thu, 05 Dec 2019 15:27:01: #1 user defined the maximum tags... INFO @ Thu, 05 Dec 2019 15:27:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Dec 2019 15:27:02: #1 tags after filtering in treatment: 55130314 INFO @ Thu, 05 Dec 2019 15:27:02: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Dec 2019 15:27:02: #1 finished! INFO @ Thu, 05 Dec 2019 15:27:02: #2 Build Peak Model... INFO @ Thu, 05 Dec 2019 15:27:02: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Dec 2019 15:27:05: #2 number of paired peaks: 0 WARNING @ Thu, 05 Dec 2019 15:27:05: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Dec 2019 15:27:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX7158308/SRX7158308.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7158308/SRX7158308.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7158308/SRX7158308.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX7158308/SRX7158308.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。