Job ID = 5721263 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 10,858,467 reads read : 10,858,467 reads written : 10,858,467 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR1581492.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:47 10858467 reads; of these: 10858467 (100.00%) were unpaired; of these: 10118793 (93.19%) aligned 0 times 559120 (5.15%) aligned exactly 1 time 180554 (1.66%) aligned >1 times 6.81% overall alignment rate Time searching: 00:00:47 Overall time: 00:00:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 384636 / 739674 = 0.5200 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 06:09:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX706811/SRX706811.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX706811/SRX706811.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX706811/SRX706811.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX706811/SRX706811.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 06:09:13: #1 read tag files... INFO @ Thu, 16 Apr 2020 06:09:13: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 06:09:15: #1 tag size is determined as 44 bps INFO @ Thu, 16 Apr 2020 06:09:15: #1 tag size = 44 INFO @ Thu, 16 Apr 2020 06:09:15: #1 total tags in treatment: 355038 INFO @ Thu, 16 Apr 2020 06:09:15: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 06:09:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 06:09:15: #1 tags after filtering in treatment: 355038 INFO @ Thu, 16 Apr 2020 06:09:15: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 16 Apr 2020 06:09:15: #1 finished! INFO @ Thu, 16 Apr 2020 06:09:15: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 06:09:15: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 06:09:15: #2 number of paired peaks: 776 WARNING @ Thu, 16 Apr 2020 06:09:15: Fewer paired peaks (776) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 776 pairs to build model! INFO @ Thu, 16 Apr 2020 06:09:15: start model_add_line... INFO @ Thu, 16 Apr 2020 06:09:15: start X-correlation... INFO @ Thu, 16 Apr 2020 06:09:15: end of X-cor INFO @ Thu, 16 Apr 2020 06:09:15: #2 finished! INFO @ Thu, 16 Apr 2020 06:09:15: #2 predicted fragment length is 39 bps INFO @ Thu, 16 Apr 2020 06:09:15: #2 alternative fragment length(s) may be 39,198,260,285,345,415,463,513,541,579 bps INFO @ Thu, 16 Apr 2020 06:09:15: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX706811/SRX706811.05_model.r WARNING @ Thu, 16 Apr 2020 06:09:15: #2 Since the d (39) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 06:09:15: #2 You may need to consider one of the other alternative d(s): 39,198,260,285,345,415,463,513,541,579 WARNING @ Thu, 16 Apr 2020 06:09:15: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 06:09:15: #3 Call peaks... INFO @ Thu, 16 Apr 2020 06:09:15: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 06:09:15: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 06:09:16: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX706811/SRX706811.05_peaks.xls INFO @ Thu, 16 Apr 2020 06:09:16: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX706811/SRX706811.05_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 06:09:16: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX706811/SRX706811.05_summits.bed INFO @ Thu, 16 Apr 2020 06:09:16: Done! pass1 - making usageList (10 chroms): 0 millis pass2 - checking and writing primary data (135 records, 4 fields): 1 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 06:09:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX706811/SRX706811.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX706811/SRX706811.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX706811/SRX706811.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX706811/SRX706811.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 06:09:43: #1 read tag files... INFO @ Thu, 16 Apr 2020 06:09:43: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 06:09:45: #1 tag size is determined as 44 bps INFO @ Thu, 16 Apr 2020 06:09:45: #1 tag size = 44 INFO @ Thu, 16 Apr 2020 06:09:45: #1 total tags in treatment: 355038 INFO @ Thu, 16 Apr 2020 06:09:45: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 06:09:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 06:09:45: #1 tags after filtering in treatment: 355038 INFO @ Thu, 16 Apr 2020 06:09:45: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 16 Apr 2020 06:09:45: #1 finished! INFO @ Thu, 16 Apr 2020 06:09:45: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 06:09:45: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 06:09:45: #2 number of paired peaks: 776 WARNING @ Thu, 16 Apr 2020 06:09:45: Fewer paired peaks (776) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 776 pairs to build model! INFO @ Thu, 16 Apr 2020 06:09:45: start model_add_line... INFO @ Thu, 16 Apr 2020 06:09:45: start X-correlation... INFO @ Thu, 16 Apr 2020 06:09:45: end of X-cor INFO @ Thu, 16 Apr 2020 06:09:45: #2 finished! INFO @ Thu, 16 Apr 2020 06:09:45: #2 predicted fragment length is 39 bps INFO @ Thu, 16 Apr 2020 06:09:45: #2 alternative fragment length(s) may be 39,198,260,285,345,415,463,513,541,579 bps INFO @ Thu, 16 Apr 2020 06:09:45: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX706811/SRX706811.10_model.r WARNING @ Thu, 16 Apr 2020 06:09:45: #2 Since the d (39) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 06:09:45: #2 You may need to consider one of the other alternative d(s): 39,198,260,285,345,415,463,513,541,579 WARNING @ Thu, 16 Apr 2020 06:09:45: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 06:09:45: #3 Call peaks... INFO @ Thu, 16 Apr 2020 06:09:45: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 06:09:46: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 06:09:46: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX706811/SRX706811.10_peaks.xls INFO @ Thu, 16 Apr 2020 06:09:46: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX706811/SRX706811.10_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 06:09:46: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX706811/SRX706811.10_summits.bed INFO @ Thu, 16 Apr 2020 06:09:46: Done! pass1 - making usageList (10 chroms): 1 millis pass2 - checking and writing primary data (67 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 06:10:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX706811/SRX706811.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX706811/SRX706811.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX706811/SRX706811.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX706811/SRX706811.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 06:10:13: #1 read tag files... INFO @ Thu, 16 Apr 2020 06:10:13: #1 read treatment tags... BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 16 Apr 2020 06:10:15: #1 tag size is determined as 44 bps INFO @ Thu, 16 Apr 2020 06:10:15: #1 tag size = 44 INFO @ Thu, 16 Apr 2020 06:10:15: #1 total tags in treatment: 355038 INFO @ Thu, 16 Apr 2020 06:10:15: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 06:10:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 06:10:15: #1 tags after filtering in treatment: 355038 INFO @ Thu, 16 Apr 2020 06:10:15: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 16 Apr 2020 06:10:15: #1 finished! INFO @ Thu, 16 Apr 2020 06:10:15: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 06:10:15: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 06:10:15: #2 number of paired peaks: 776 WARNING @ Thu, 16 Apr 2020 06:10:15: Fewer paired peaks (776) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 776 pairs to build model! INFO @ Thu, 16 Apr 2020 06:10:15: start model_add_line... INFO @ Thu, 16 Apr 2020 06:10:15: start X-correlation... INFO @ Thu, 16 Apr 2020 06:10:15: end of X-cor INFO @ Thu, 16 Apr 2020 06:10:15: #2 finished! INFO @ Thu, 16 Apr 2020 06:10:15: #2 predicted fragment length is 39 bps INFO @ Thu, 16 Apr 2020 06:10:15: #2 alternative fragment length(s) may be 39,198,260,285,345,415,463,513,541,579 bps INFO @ Thu, 16 Apr 2020 06:10:15: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX706811/SRX706811.20_model.r WARNING @ Thu, 16 Apr 2020 06:10:15: #2 Since the d (39) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 06:10:15: #2 You may need to consider one of the other alternative d(s): 39,198,260,285,345,415,463,513,541,579 WARNING @ Thu, 16 Apr 2020 06:10:15: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 06:10:15: #3 Call peaks... INFO @ Thu, 16 Apr 2020 06:10:15: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Thu, 16 Apr 2020 06:10:15: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 06:10:16: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX706811/SRX706811.20_peaks.xls INFO @ Thu, 16 Apr 2020 06:10:16: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX706811/SRX706811.20_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 06:10:16: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX706811/SRX706811.20_summits.bed INFO @ Thu, 16 Apr 2020 06:10:16: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (34 records, 4 fields): 1 millis CompletedMACS2peakCalling