Job ID = 9159823 sra ファイルのダウンロード中... Completed: 1068340K bytes transferred in 11 seconds (734341K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 50693654 spots for /home/okishinya/chipatlas/results/dm3/SRX699544/SRR1573543.sra Written 50693654 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:41 50693654 reads; of these: 50693654 (100.00%) were unpaired; of these: 9888397 (19.51%) aligned 0 times 36911289 (72.81%) aligned exactly 1 time 3893968 (7.68%) aligned >1 times 80.49% overall alignment rate Time searching: 00:10:41 Overall time: 00:10:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5321305 / 40805257 = 0.1304 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 00:20:45: # Command line: callpeak -t SRX699544.bam -f BAM -g dm -n SRX699544.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX699544.05 # format = BAM # ChIP-seq file = ['SRX699544.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 00:20:45: #1 read tag files... INFO @ Wed, 28 Jun 2017 00:20:45: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 00:20:45: # Command line: callpeak -t SRX699544.bam -f BAM -g dm -n SRX699544.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX699544.10 # format = BAM # ChIP-seq file = ['SRX699544.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 00:20:45: #1 read tag files... INFO @ Wed, 28 Jun 2017 00:20:45: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 00:20:45: # Command line: callpeak -t SRX699544.bam -f BAM -g dm -n SRX699544.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX699544.20 # format = BAM # ChIP-seq file = ['SRX699544.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 00:20:45: #1 read tag files... INFO @ Wed, 28 Jun 2017 00:20:45: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 00:20:52: 1000000 INFO @ Wed, 28 Jun 2017 00:20:53: 1000000 INFO @ Wed, 28 Jun 2017 00:20:53: 1000000 INFO @ Wed, 28 Jun 2017 00:20:58: 2000000 INFO @ Wed, 28 Jun 2017 00:21:00: 2000000 INFO @ Wed, 28 Jun 2017 00:21:00: 2000000 INFO @ Wed, 28 Jun 2017 00:21:05: 3000000 INFO @ Wed, 28 Jun 2017 00:21:07: 3000000 INFO @ Wed, 28 Jun 2017 00:21:07: 3000000 INFO @ Wed, 28 Jun 2017 00:21:11: 4000000 INFO @ Wed, 28 Jun 2017 00:21:15: 4000000 INFO @ Wed, 28 Jun 2017 00:21:15: 4000000 INFO @ Wed, 28 Jun 2017 00:21:18: 5000000 INFO @ Wed, 28 Jun 2017 00:21:22: 5000000 INFO @ Wed, 28 Jun 2017 00:21:22: 5000000 INFO @ Wed, 28 Jun 2017 00:21:25: 6000000 INFO @ Wed, 28 Jun 2017 00:21:30: 6000000 INFO @ Wed, 28 Jun 2017 00:21:30: 6000000 INFO @ Wed, 28 Jun 2017 00:21:31: 7000000 INFO @ Wed, 28 Jun 2017 00:21:37: 7000000 INFO @ Wed, 28 Jun 2017 00:21:37: 7000000 INFO @ Wed, 28 Jun 2017 00:21:38: 8000000 INFO @ Wed, 28 Jun 2017 00:21:44: 9000000 INFO @ Wed, 28 Jun 2017 00:21:44: 8000000 INFO @ Wed, 28 Jun 2017 00:21:44: 8000000 INFO @ Wed, 28 Jun 2017 00:21:51: 10000000 INFO @ Wed, 28 Jun 2017 00:21:52: 9000000 INFO @ Wed, 28 Jun 2017 00:21:52: 9000000 INFO @ Wed, 28 Jun 2017 00:21:57: 11000000 INFO @ Wed, 28 Jun 2017 00:21:59: 10000000 INFO @ Wed, 28 Jun 2017 00:21:59: 10000000 INFO @ Wed, 28 Jun 2017 00:22:04: 12000000 INFO @ Wed, 28 Jun 2017 00:22:06: 11000000 INFO @ Wed, 28 Jun 2017 00:22:06: 11000000 INFO @ Wed, 28 Jun 2017 00:22:10: 13000000 INFO @ Wed, 28 Jun 2017 00:22:14: 12000000 INFO @ Wed, 28 Jun 2017 00:22:14: 12000000 INFO @ Wed, 28 Jun 2017 00:22:17: 14000000 INFO @ Wed, 28 Jun 2017 00:22:21: 13000000 INFO @ Wed, 28 Jun 2017 00:22:21: 13000000 INFO @ Wed, 28 Jun 2017 00:22:24: 15000000 INFO @ Wed, 28 Jun 2017 00:22:29: 14000000 INFO @ Wed, 28 Jun 2017 00:22:29: 14000000 INFO @ Wed, 28 Jun 2017 00:22:30: 16000000 INFO @ Wed, 28 Jun 2017 00:22:36: 15000000 INFO @ Wed, 28 Jun 2017 00:22:36: 15000000 INFO @ Wed, 28 Jun 2017 00:22:37: 17000000 INFO @ Wed, 28 Jun 2017 00:22:43: 16000000 INFO @ Wed, 28 Jun 2017 00:22:43: 16000000 INFO @ Wed, 28 Jun 2017 00:22:44: 18000000 INFO @ Wed, 28 Jun 2017 00:22:50: 19000000 INFO @ Wed, 28 Jun 2017 00:22:51: 17000000 INFO @ Wed, 28 Jun 2017 00:22:51: 17000000 INFO @ Wed, 28 Jun 2017 00:22:57: 20000000 INFO @ Wed, 28 Jun 2017 00:22:58: 18000000 INFO @ Wed, 28 Jun 2017 00:22:58: 18000000 INFO @ Wed, 28 Jun 2017 00:23:04: 21000000 INFO @ Wed, 28 Jun 2017 00:23:05: 19000000 INFO @ Wed, 28 Jun 2017 00:23:05: 19000000 INFO @ Wed, 28 Jun 2017 00:23:10: 22000000 INFO @ Wed, 28 Jun 2017 00:23:13: 20000000 INFO @ Wed, 28 Jun 2017 00:23:13: 20000000 INFO @ Wed, 28 Jun 2017 00:23:17: 23000000 INFO @ Wed, 28 Jun 2017 00:23:20: 21000000 INFO @ Wed, 28 Jun 2017 00:23:20: 21000000 INFO @ Wed, 28 Jun 2017 00:23:23: 24000000 INFO @ Wed, 28 Jun 2017 00:23:28: 22000000 INFO @ Wed, 28 Jun 2017 00:23:28: 22000000 INFO @ Wed, 28 Jun 2017 00:23:29: 25000000 INFO @ Wed, 28 Jun 2017 00:23:35: 26000000 INFO @ Wed, 28 Jun 2017 00:23:35: 23000000 INFO @ Wed, 28 Jun 2017 00:23:35: 23000000 INFO @ Wed, 28 Jun 2017 00:23:41: 27000000 INFO @ Wed, 28 Jun 2017 00:23:43: 24000000 INFO @ Wed, 28 Jun 2017 00:23:43: 24000000 INFO @ Wed, 28 Jun 2017 00:23:47: 28000000 INFO @ Wed, 28 Jun 2017 00:23:50: 25000000 INFO @ Wed, 28 Jun 2017 00:23:50: 25000000 INFO @ Wed, 28 Jun 2017 00:23:53: 29000000 INFO @ Wed, 28 Jun 2017 00:23:58: 26000000 INFO @ Wed, 28 Jun 2017 00:23:58: 26000000 INFO @ Wed, 28 Jun 2017 00:23:59: 30000000 INFO @ Wed, 28 Jun 2017 00:24:05: 27000000 INFO @ Wed, 28 Jun 2017 00:24:05: 27000000 INFO @ Wed, 28 Jun 2017 00:24:05: 31000000 INFO @ Wed, 28 Jun 2017 00:24:12: 32000000 INFO @ Wed, 28 Jun 2017 00:24:13: 28000000 INFO @ Wed, 28 Jun 2017 00:24:13: 28000000 INFO @ Wed, 28 Jun 2017 00:24:18: 33000000 INFO @ Wed, 28 Jun 2017 00:24:20: 29000000 INFO @ Wed, 28 Jun 2017 00:24:20: 29000000 INFO @ Wed, 28 Jun 2017 00:24:24: 34000000 INFO @ Wed, 28 Jun 2017 00:24:28: 30000000 INFO @ Wed, 28 Jun 2017 00:24:28: 30000000 INFO @ Wed, 28 Jun 2017 00:24:31: 35000000 INFO @ Wed, 28 Jun 2017 00:24:35: #1 tag size is determined as 36 bps INFO @ Wed, 28 Jun 2017 00:24:35: #1 tag size = 36 INFO @ Wed, 28 Jun 2017 00:24:35: #1 total tags in treatment: 35483952 INFO @ Wed, 28 Jun 2017 00:24:35: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 00:24:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 00:24:35: 31000000 INFO @ Wed, 28 Jun 2017 00:24:36: 31000000 INFO @ Wed, 28 Jun 2017 00:24:36: #1 tags after filtering in treatment: 35483952 INFO @ Wed, 28 Jun 2017 00:24:36: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 00:24:36: #1 finished! INFO @ Wed, 28 Jun 2017 00:24:36: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 00:24:36: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 00:24:38: #2 number of paired peaks: 14 WARNING @ Wed, 28 Jun 2017 00:24:38: Too few paired peaks (14) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 00:24:38: Process for pairing-model is terminated! cat: SRX699544.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX699544.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX699544.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX699544.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 00:24:43: 32000000 INFO @ Wed, 28 Jun 2017 00:24:43: 32000000 INFO @ Wed, 28 Jun 2017 00:24:50: 33000000 INFO @ Wed, 28 Jun 2017 00:24:51: 33000000 INFO @ Wed, 28 Jun 2017 00:24:58: 34000000 INFO @ Wed, 28 Jun 2017 00:24:58: 34000000 INFO @ Wed, 28 Jun 2017 00:25:05: 35000000 INFO @ Wed, 28 Jun 2017 00:25:06: 35000000 INFO @ Wed, 28 Jun 2017 00:25:09: #1 tag size is determined as 36 bps INFO @ Wed, 28 Jun 2017 00:25:09: #1 tag size = 36 INFO @ Wed, 28 Jun 2017 00:25:09: #1 total tags in treatment: 35483952 INFO @ Wed, 28 Jun 2017 00:25:09: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 00:25:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 00:25:10: #1 tag size is determined as 36 bps INFO @ Wed, 28 Jun 2017 00:25:10: #1 tag size = 36 INFO @ Wed, 28 Jun 2017 00:25:10: #1 total tags in treatment: 35483952 INFO @ Wed, 28 Jun 2017 00:25:10: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 00:25:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 00:25:10: #1 tags after filtering in treatment: 35483952 INFO @ Wed, 28 Jun 2017 00:25:10: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 00:25:10: #1 finished! INFO @ Wed, 28 Jun 2017 00:25:10: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 00:25:10: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 00:25:10: #1 tags after filtering in treatment: 35483952 INFO @ Wed, 28 Jun 2017 00:25:10: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 00:25:10: #1 finished! INFO @ Wed, 28 Jun 2017 00:25:10: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 00:25:10: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 00:25:12: #2 number of paired peaks: 14 WARNING @ Wed, 28 Jun 2017 00:25:12: Too few paired peaks (14) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 00:25:12: Process for pairing-model is terminated! cat: SRX699544.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX699544.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX699544.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX699544.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 00:25:13: #2 number of paired peaks: 14 WARNING @ Wed, 28 Jun 2017 00:25:13: Too few paired peaks (14) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 00:25:13: Process for pairing-model is terminated! cat: SRX699544.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX699544.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX699544.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX699544.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。