Job ID = 6528470 SRX = SRX6962950 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T15:11:21 prefetch.2.10.7: 1) Downloading 'SRR10244307'... 2020-06-29T15:11:21 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:13:41 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:13:42 prefetch.2.10.7: 'SRR10244307' is valid 2020-06-29T15:13:42 prefetch.2.10.7: 1) 'SRR10244307' was downloaded successfully 2020-06-29T15:13:42 prefetch.2.10.7: 'SRR10244307' has 0 unresolved dependencies Read 12405101 spots for SRR10244307/SRR10244307.sra Written 12405101 spots for SRR10244307/SRR10244307.sra 2020-06-29T15:14:38 prefetch.2.10.7: 1) Downloading 'SRR10244308'... 2020-06-29T15:14:38 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:16:02 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:16:02 prefetch.2.10.7: 'SRR10244308' is valid 2020-06-29T15:16:02 prefetch.2.10.7: 1) 'SRR10244308' was downloaded successfully 2020-06-29T15:16:02 prefetch.2.10.7: 'SRR10244308' has 0 unresolved dependencies Read 12283986 spots for SRR10244308/SRR10244308.sra Written 12283986 spots for SRR10244308/SRR10244308.sra 2020-06-29T15:16:56 prefetch.2.10.7: 1) Downloading 'SRR10244309'... 2020-06-29T15:16:56 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:18:33 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:18:34 prefetch.2.10.7: 'SRR10244309' is valid 2020-06-29T15:18:34 prefetch.2.10.7: 1) 'SRR10244309' was downloaded successfully 2020-06-29T15:18:34 prefetch.2.10.7: 'SRR10244309' has 0 unresolved dependencies Read 12957467 spots for SRR10244309/SRR10244309.sra Written 12957467 spots for SRR10244309/SRR10244309.sra 2020-06-29T15:19:28 prefetch.2.10.7: 1) Downloading 'SRR10244310'... 2020-06-29T15:19:28 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:21:03 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:21:04 prefetch.2.10.7: 'SRR10244310' is valid 2020-06-29T15:21:04 prefetch.2.10.7: 1) 'SRR10244310' was downloaded successfully 2020-06-29T15:21:04 prefetch.2.10.7: 'SRR10244310' has 0 unresolved dependencies Read 12832569 spots for SRR10244310/SRR10244310.sra Written 12832569 spots for SRR10244310/SRR10244310.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:20:53 50479123 reads; of these: 50479123 (100.00%) were unpaired; of these: 4379214 (8.68%) aligned 0 times 34130879 (67.61%) aligned exactly 1 time 11969030 (23.71%) aligned >1 times 91.32% overall alignment rate Time searching: 00:20:53 Overall time: 00:20:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 11926363 / 46099909 = 0.2587 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:15:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6962950/SRX6962950.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6962950/SRX6962950.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6962950/SRX6962950.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6962950/SRX6962950.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:15:26: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:15:26: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:15:31: 1000000 INFO @ Tue, 30 Jun 2020 01:15:36: 2000000 INFO @ Tue, 30 Jun 2020 01:15:42: 3000000 INFO @ Tue, 30 Jun 2020 01:15:47: 4000000 INFO @ Tue, 30 Jun 2020 01:15:52: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:15:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6962950/SRX6962950.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6962950/SRX6962950.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6962950/SRX6962950.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6962950/SRX6962950.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:15:56: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:15:56: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:15:57: 6000000 INFO @ Tue, 30 Jun 2020 01:16:02: 1000000 INFO @ Tue, 30 Jun 2020 01:16:03: 7000000 INFO @ Tue, 30 Jun 2020 01:16:08: 8000000 INFO @ Tue, 30 Jun 2020 01:16:09: 2000000 INFO @ Tue, 30 Jun 2020 01:16:13: 9000000 INFO @ Tue, 30 Jun 2020 01:16:15: 3000000 INFO @ Tue, 30 Jun 2020 01:16:19: 10000000 INFO @ Tue, 30 Jun 2020 01:16:21: 4000000 INFO @ Tue, 30 Jun 2020 01:16:24: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:16:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6962950/SRX6962950.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6962950/SRX6962950.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6962950/SRX6962950.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6962950/SRX6962950.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:16:26: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:16:26: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:16:28: 5000000 INFO @ Tue, 30 Jun 2020 01:16:29: 12000000 INFO @ Tue, 30 Jun 2020 01:16:32: 1000000 INFO @ Tue, 30 Jun 2020 01:16:34: 6000000 INFO @ Tue, 30 Jun 2020 01:16:35: 13000000 INFO @ Tue, 30 Jun 2020 01:16:37: 2000000 INFO @ Tue, 30 Jun 2020 01:16:40: 7000000 INFO @ Tue, 30 Jun 2020 01:16:40: 14000000 INFO @ Tue, 30 Jun 2020 01:16:42: 3000000 INFO @ Tue, 30 Jun 2020 01:16:46: 15000000 INFO @ Tue, 30 Jun 2020 01:16:46: 8000000 INFO @ Tue, 30 Jun 2020 01:16:48: 4000000 INFO @ Tue, 30 Jun 2020 01:16:51: 16000000 INFO @ Tue, 30 Jun 2020 01:16:52: 9000000 INFO @ Tue, 30 Jun 2020 01:16:53: 5000000 INFO @ Tue, 30 Jun 2020 01:16:56: 17000000 INFO @ Tue, 30 Jun 2020 01:16:59: 6000000 INFO @ Tue, 30 Jun 2020 01:16:59: 10000000 INFO @ Tue, 30 Jun 2020 01:17:02: 18000000 INFO @ Tue, 30 Jun 2020 01:17:04: 7000000 INFO @ Tue, 30 Jun 2020 01:17:05: 11000000 INFO @ Tue, 30 Jun 2020 01:17:07: 19000000 INFO @ Tue, 30 Jun 2020 01:17:09: 8000000 INFO @ Tue, 30 Jun 2020 01:17:11: 12000000 INFO @ Tue, 30 Jun 2020 01:17:12: 20000000 INFO @ Tue, 30 Jun 2020 01:17:15: 9000000 INFO @ Tue, 30 Jun 2020 01:17:18: 13000000 INFO @ Tue, 30 Jun 2020 01:17:18: 21000000 INFO @ Tue, 30 Jun 2020 01:17:20: 10000000 INFO @ Tue, 30 Jun 2020 01:17:23: 22000000 INFO @ Tue, 30 Jun 2020 01:17:24: 14000000 INFO @ Tue, 30 Jun 2020 01:17:26: 11000000 INFO @ Tue, 30 Jun 2020 01:17:28: 23000000 INFO @ Tue, 30 Jun 2020 01:17:31: 15000000 INFO @ Tue, 30 Jun 2020 01:17:31: 12000000 INFO @ Tue, 30 Jun 2020 01:17:33: 24000000 INFO @ Tue, 30 Jun 2020 01:17:36: 13000000 INFO @ Tue, 30 Jun 2020 01:17:37: 16000000 INFO @ Tue, 30 Jun 2020 01:17:39: 25000000 INFO @ Tue, 30 Jun 2020 01:17:42: 14000000 INFO @ Tue, 30 Jun 2020 01:17:43: 17000000 INFO @ Tue, 30 Jun 2020 01:17:44: 26000000 INFO @ Tue, 30 Jun 2020 01:17:47: 15000000 INFO @ Tue, 30 Jun 2020 01:17:49: 27000000 INFO @ Tue, 30 Jun 2020 01:17:50: 18000000 INFO @ Tue, 30 Jun 2020 01:17:53: 16000000 INFO @ Tue, 30 Jun 2020 01:17:55: 28000000 INFO @ Tue, 30 Jun 2020 01:17:56: 19000000 INFO @ Tue, 30 Jun 2020 01:17:58: 17000000 INFO @ Tue, 30 Jun 2020 01:18:00: 29000000 INFO @ Tue, 30 Jun 2020 01:18:02: 20000000 INFO @ Tue, 30 Jun 2020 01:18:03: 18000000 INFO @ Tue, 30 Jun 2020 01:18:05: 30000000 INFO @ Tue, 30 Jun 2020 01:18:09: 19000000 INFO @ Tue, 30 Jun 2020 01:18:09: 21000000 INFO @ Tue, 30 Jun 2020 01:18:10: 31000000 INFO @ Tue, 30 Jun 2020 01:18:14: 20000000 INFO @ Tue, 30 Jun 2020 01:18:15: 22000000 INFO @ Tue, 30 Jun 2020 01:18:16: 32000000 INFO @ Tue, 30 Jun 2020 01:18:20: 21000000 INFO @ Tue, 30 Jun 2020 01:18:21: 33000000 INFO @ Tue, 30 Jun 2020 01:18:21: 23000000 INFO @ Tue, 30 Jun 2020 01:18:25: 22000000 INFO @ Tue, 30 Jun 2020 01:18:26: 34000000 INFO @ Tue, 30 Jun 2020 01:18:27: 24000000 INFO @ Tue, 30 Jun 2020 01:18:28: #1 tag size is determined as 75 bps INFO @ Tue, 30 Jun 2020 01:18:28: #1 tag size = 75 INFO @ Tue, 30 Jun 2020 01:18:28: #1 total tags in treatment: 34173546 INFO @ Tue, 30 Jun 2020 01:18:28: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:18:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:18:28: #1 tags after filtering in treatment: 34173546 INFO @ Tue, 30 Jun 2020 01:18:28: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:18:28: #1 finished! INFO @ Tue, 30 Jun 2020 01:18:28: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:18:28: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:18:30: 23000000 INFO @ Tue, 30 Jun 2020 01:18:30: #2 number of paired peaks: 2 WARNING @ Tue, 30 Jun 2020 01:18:30: Too few paired peaks (2) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:18:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6962950/SRX6962950.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6962950/SRX6962950.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6962950/SRX6962950.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6962950/SRX6962950.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 01:18:34: 25000000 INFO @ Tue, 30 Jun 2020 01:18:35: 24000000 INFO @ Tue, 30 Jun 2020 01:18:40: 26000000 INFO @ Tue, 30 Jun 2020 01:18:41: 25000000 INFO @ Tue, 30 Jun 2020 01:18:46: 27000000 INFO @ Tue, 30 Jun 2020 01:18:46: 26000000 INFO @ Tue, 30 Jun 2020 01:18:51: 27000000 INFO @ Tue, 30 Jun 2020 01:18:52: 28000000 INFO @ Tue, 30 Jun 2020 01:18:57: 28000000 INFO @ Tue, 30 Jun 2020 01:18:58: 29000000 INFO @ Tue, 30 Jun 2020 01:19:02: 29000000 INFO @ Tue, 30 Jun 2020 01:19:04: 30000000 INFO @ Tue, 30 Jun 2020 01:19:07: 30000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 01:19:10: 31000000 INFO @ Tue, 30 Jun 2020 01:19:13: 31000000 INFO @ Tue, 30 Jun 2020 01:19:16: 32000000 INFO @ Tue, 30 Jun 2020 01:19:18: 32000000 INFO @ Tue, 30 Jun 2020 01:19:22: 33000000 INFO @ Tue, 30 Jun 2020 01:19:23: 33000000 INFO @ Tue, 30 Jun 2020 01:19:28: 34000000 INFO @ Tue, 30 Jun 2020 01:19:28: 34000000 INFO @ Tue, 30 Jun 2020 01:19:29: #1 tag size is determined as 75 bps INFO @ Tue, 30 Jun 2020 01:19:29: #1 tag size = 75 INFO @ Tue, 30 Jun 2020 01:19:29: #1 total tags in treatment: 34173546 INFO @ Tue, 30 Jun 2020 01:19:29: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:19:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:19:30: #1 tags after filtering in treatment: 34173546 INFO @ Tue, 30 Jun 2020 01:19:30: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:19:30: #1 finished! INFO @ Tue, 30 Jun 2020 01:19:30: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:19:30: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:19:30: #1 tag size is determined as 75 bps INFO @ Tue, 30 Jun 2020 01:19:30: #1 tag size = 75 INFO @ Tue, 30 Jun 2020 01:19:30: #1 total tags in treatment: 34173546 INFO @ Tue, 30 Jun 2020 01:19:30: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:19:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:19:30: #1 tags after filtering in treatment: 34173546 INFO @ Tue, 30 Jun 2020 01:19:30: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:19:30: #1 finished! INFO @ Tue, 30 Jun 2020 01:19:30: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:19:30: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:19:32: #2 number of paired peaks: 2 WARNING @ Tue, 30 Jun 2020 01:19:32: Too few paired peaks (2) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:19:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6962950/SRX6962950.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6962950/SRX6962950.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6962950/SRX6962950.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6962950/SRX6962950.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 01:19:32: #2 number of paired peaks: 2 WARNING @ Tue, 30 Jun 2020 01:19:32: Too few paired peaks (2) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:19:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6962950/SRX6962950.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6962950/SRX6962950.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6962950/SRX6962950.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6962950/SRX6962950.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。