Job ID = 6528469 SRX = SRX6962949 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T15:06:20 prefetch.2.10.7: 1) Downloading 'SRR10244303'... 2020-06-29T15:06:20 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:08:00 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:08:01 prefetch.2.10.7: 'SRR10244303' is valid 2020-06-29T15:08:01 prefetch.2.10.7: 1) 'SRR10244303' was downloaded successfully 2020-06-29T15:08:01 prefetch.2.10.7: 'SRR10244303' has 0 unresolved dependencies Read 11826452 spots for SRR10244303/SRR10244303.sra Written 11826452 spots for SRR10244303/SRR10244303.sra 2020-06-29T15:08:51 prefetch.2.10.7: 1) Downloading 'SRR10244304'... 2020-06-29T15:08:51 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:10:17 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:10:18 prefetch.2.10.7: 'SRR10244304' is valid 2020-06-29T15:10:18 prefetch.2.10.7: 1) 'SRR10244304' was downloaded successfully 2020-06-29T15:10:18 prefetch.2.10.7: 'SRR10244304' has 0 unresolved dependencies Read 11725216 spots for SRR10244304/SRR10244304.sra Written 11725216 spots for SRR10244304/SRR10244304.sra 2020-06-29T15:11:08 prefetch.2.10.7: 1) Downloading 'SRR10244305'... 2020-06-29T15:11:08 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:12:24 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:12:24 prefetch.2.10.7: 'SRR10244305' is valid 2020-06-29T15:12:24 prefetch.2.10.7: 1) 'SRR10244305' was downloaded successfully 2020-06-29T15:12:25 prefetch.2.10.7: 'SRR10244305' has 0 unresolved dependencies Read 12350191 spots for SRR10244305/SRR10244305.sra Written 12350191 spots for SRR10244305/SRR10244305.sra 2020-06-29T15:13:21 prefetch.2.10.7: 1) Downloading 'SRR10244306'... 2020-06-29T15:13:21 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:15:20 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:15:21 prefetch.2.10.7: 'SRR10244306' is valid 2020-06-29T15:15:21 prefetch.2.10.7: 1) 'SRR10244306' was downloaded successfully 2020-06-29T15:15:21 prefetch.2.10.7: 'SRR10244306' has 0 unresolved dependencies Read 12233190 spots for SRR10244306/SRR10244306.sra Written 12233190 spots for SRR10244306/SRR10244306.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:28 48135049 reads; of these: 48135049 (100.00%) were unpaired; of these: 29989754 (62.30%) aligned 0 times 13433270 (27.91%) aligned exactly 1 time 4712025 (9.79%) aligned >1 times 37.70% overall alignment rate Time searching: 00:11:28 Overall time: 00:11:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6001842 / 18145295 = 0.3308 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:51:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6962949/SRX6962949.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6962949/SRX6962949.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6962949/SRX6962949.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6962949/SRX6962949.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:51:36: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:51:36: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:51:41: 1000000 INFO @ Tue, 30 Jun 2020 00:51:46: 2000000 INFO @ Tue, 30 Jun 2020 00:51:52: 3000000 INFO @ Tue, 30 Jun 2020 00:51:57: 4000000 INFO @ Tue, 30 Jun 2020 00:52:02: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:52:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6962949/SRX6962949.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6962949/SRX6962949.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6962949/SRX6962949.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6962949/SRX6962949.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:52:05: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:52:05: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:52:08: 6000000 INFO @ Tue, 30 Jun 2020 00:52:11: 1000000 INFO @ Tue, 30 Jun 2020 00:52:13: 7000000 INFO @ Tue, 30 Jun 2020 00:52:17: 2000000 INFO @ Tue, 30 Jun 2020 00:52:19: 8000000 INFO @ Tue, 30 Jun 2020 00:52:22: 3000000 INFO @ Tue, 30 Jun 2020 00:52:24: 9000000 INFO @ Tue, 30 Jun 2020 00:52:28: 4000000 INFO @ Tue, 30 Jun 2020 00:52:30: 10000000 INFO @ Tue, 30 Jun 2020 00:52:33: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:52:36: 11000000 INFO @ Tue, 30 Jun 2020 00:52:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6962949/SRX6962949.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6962949/SRX6962949.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6962949/SRX6962949.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6962949/SRX6962949.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:52:36: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:52:36: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:52:39: 6000000 INFO @ Tue, 30 Jun 2020 00:52:41: 12000000 INFO @ Tue, 30 Jun 2020 00:52:42: 1000000 INFO @ Tue, 30 Jun 2020 00:52:42: #1 tag size is determined as 75 bps INFO @ Tue, 30 Jun 2020 00:52:42: #1 tag size = 75 INFO @ Tue, 30 Jun 2020 00:52:42: #1 total tags in treatment: 12143453 INFO @ Tue, 30 Jun 2020 00:52:42: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:52:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:52:42: #1 tags after filtering in treatment: 12143453 INFO @ Tue, 30 Jun 2020 00:52:42: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:52:42: #1 finished! INFO @ Tue, 30 Jun 2020 00:52:42: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:52:42: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:52:43: #2 number of paired peaks: 45 WARNING @ Tue, 30 Jun 2020 00:52:43: Too few paired peaks (45) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:52:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6962949/SRX6962949.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6962949/SRX6962949.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6962949/SRX6962949.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6962949/SRX6962949.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:52:45: 7000000 INFO @ Tue, 30 Jun 2020 00:52:47: 2000000 INFO @ Tue, 30 Jun 2020 00:52:50: 8000000 INFO @ Tue, 30 Jun 2020 00:52:53: 3000000 INFO @ Tue, 30 Jun 2020 00:52:56: 9000000 INFO @ Tue, 30 Jun 2020 00:52:59: 4000000 INFO @ Tue, 30 Jun 2020 00:53:02: 10000000 INFO @ Tue, 30 Jun 2020 00:53:04: 5000000 INFO @ Tue, 30 Jun 2020 00:53:07: 11000000 INFO @ Tue, 30 Jun 2020 00:53:11: 6000000 INFO @ Tue, 30 Jun 2020 00:53:13: 12000000 INFO @ Tue, 30 Jun 2020 00:53:14: #1 tag size is determined as 75 bps INFO @ Tue, 30 Jun 2020 00:53:14: #1 tag size = 75 INFO @ Tue, 30 Jun 2020 00:53:14: #1 total tags in treatment: 12143453 INFO @ Tue, 30 Jun 2020 00:53:14: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:53:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:53:14: #1 tags after filtering in treatment: 12143453 INFO @ Tue, 30 Jun 2020 00:53:14: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:53:14: #1 finished! INFO @ Tue, 30 Jun 2020 00:53:14: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:53:14: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:53:15: #2 number of paired peaks: 45 WARNING @ Tue, 30 Jun 2020 00:53:15: Too few paired peaks (45) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:53:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6962949/SRX6962949.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6962949/SRX6962949.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6962949/SRX6962949.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6962949/SRX6962949.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:53:17: 7000000 INFO @ Tue, 30 Jun 2020 00:53:22: 8000000 INFO @ Tue, 30 Jun 2020 00:53:28: 9000000 INFO @ Tue, 30 Jun 2020 00:53:34: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:53:40: 11000000 INFO @ Tue, 30 Jun 2020 00:53:45: 12000000 INFO @ Tue, 30 Jun 2020 00:53:46: #1 tag size is determined as 75 bps INFO @ Tue, 30 Jun 2020 00:53:46: #1 tag size = 75 INFO @ Tue, 30 Jun 2020 00:53:46: #1 total tags in treatment: 12143453 INFO @ Tue, 30 Jun 2020 00:53:46: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:53:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:53:46: #1 tags after filtering in treatment: 12143453 INFO @ Tue, 30 Jun 2020 00:53:46: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:53:46: #1 finished! INFO @ Tue, 30 Jun 2020 00:53:46: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:53:46: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:53:47: #2 number of paired peaks: 45 WARNING @ Tue, 30 Jun 2020 00:53:47: Too few paired peaks (45) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:53:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6962949/SRX6962949.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6962949/SRX6962949.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6962949/SRX6962949.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6962949/SRX6962949.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。