Job ID = 6498676 SRX = SRX685468 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-25T23:36:15 prefetch.2.10.7: 1) Downloading 'SRR1556390'... 2020-06-25T23:36:15 prefetch.2.10.7: Downloading via HTTPS... 2020-06-25T23:43:50 prefetch.2.10.7: HTTPS download succeed 2020-06-25T23:43:50 prefetch.2.10.7: 1) 'SRR1556390' was downloaded successfully Read 53620336 spots for SRR1556390/SRR1556390.sra Written 53620336 spots for SRR1556390/SRR1556390.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:20:03 53620336 reads; of these: 53620336 (100.00%) were unpaired; of these: 1320623 (2.46%) aligned 0 times 34621746 (64.57%) aligned exactly 1 time 17677967 (32.97%) aligned >1 times 97.54% overall alignment rate Time searching: 00:20:03 Overall time: 00:20:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 10492242 / 52299713 = 0.2006 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 09:20:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX685468/SRX685468.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX685468/SRX685468.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX685468/SRX685468.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX685468/SRX685468.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 09:20:18: #1 read tag files... INFO @ Fri, 26 Jun 2020 09:20:18: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 09:20:23: 1000000 INFO @ Fri, 26 Jun 2020 09:20:28: 2000000 INFO @ Fri, 26 Jun 2020 09:20:34: 3000000 INFO @ Fri, 26 Jun 2020 09:20:39: 4000000 INFO @ Fri, 26 Jun 2020 09:20:44: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 09:20:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX685468/SRX685468.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX685468/SRX685468.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX685468/SRX685468.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX685468/SRX685468.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 09:20:48: #1 read tag files... INFO @ Fri, 26 Jun 2020 09:20:48: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 09:20:50: 6000000 INFO @ Fri, 26 Jun 2020 09:20:53: 1000000 INFO @ Fri, 26 Jun 2020 09:20:55: 7000000 INFO @ Fri, 26 Jun 2020 09:20:59: 2000000 INFO @ Fri, 26 Jun 2020 09:21:01: 8000000 INFO @ Fri, 26 Jun 2020 09:21:04: 3000000 INFO @ Fri, 26 Jun 2020 09:21:06: 9000000 INFO @ Fri, 26 Jun 2020 09:21:10: 4000000 INFO @ Fri, 26 Jun 2020 09:21:12: 10000000 INFO @ Fri, 26 Jun 2020 09:21:15: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 09:21:17: 11000000 INFO @ Fri, 26 Jun 2020 09:21:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX685468/SRX685468.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX685468/SRX685468.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX685468/SRX685468.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX685468/SRX685468.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 09:21:18: #1 read tag files... INFO @ Fri, 26 Jun 2020 09:21:18: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 09:21:21: 6000000 INFO @ Fri, 26 Jun 2020 09:21:23: 12000000 INFO @ Fri, 26 Jun 2020 09:21:24: 1000000 INFO @ Fri, 26 Jun 2020 09:21:27: 7000000 INFO @ Fri, 26 Jun 2020 09:21:28: 13000000 INFO @ Fri, 26 Jun 2020 09:21:30: 2000000 INFO @ Fri, 26 Jun 2020 09:21:33: 8000000 INFO @ Fri, 26 Jun 2020 09:21:34: 14000000 INFO @ Fri, 26 Jun 2020 09:21:36: 3000000 INFO @ Fri, 26 Jun 2020 09:21:38: 9000000 INFO @ Fri, 26 Jun 2020 09:21:40: 15000000 INFO @ Fri, 26 Jun 2020 09:21:42: 4000000 INFO @ Fri, 26 Jun 2020 09:21:44: 10000000 INFO @ Fri, 26 Jun 2020 09:21:45: 16000000 INFO @ Fri, 26 Jun 2020 09:21:48: 5000000 INFO @ Fri, 26 Jun 2020 09:21:50: 11000000 INFO @ Fri, 26 Jun 2020 09:21:51: 17000000 INFO @ Fri, 26 Jun 2020 09:21:54: 6000000 INFO @ Fri, 26 Jun 2020 09:21:56: 12000000 INFO @ Fri, 26 Jun 2020 09:21:56: 18000000 INFO @ Fri, 26 Jun 2020 09:22:00: 7000000 INFO @ Fri, 26 Jun 2020 09:22:02: 13000000 INFO @ Fri, 26 Jun 2020 09:22:02: 19000000 INFO @ Fri, 26 Jun 2020 09:22:06: 8000000 INFO @ Fri, 26 Jun 2020 09:22:07: 20000000 INFO @ Fri, 26 Jun 2020 09:22:08: 14000000 INFO @ Fri, 26 Jun 2020 09:22:12: 9000000 INFO @ Fri, 26 Jun 2020 09:22:13: 21000000 INFO @ Fri, 26 Jun 2020 09:22:13: 15000000 INFO @ Fri, 26 Jun 2020 09:22:18: 10000000 INFO @ Fri, 26 Jun 2020 09:22:19: 22000000 INFO @ Fri, 26 Jun 2020 09:22:19: 16000000 INFO @ Fri, 26 Jun 2020 09:22:24: 11000000 INFO @ Fri, 26 Jun 2020 09:22:24: 23000000 INFO @ Fri, 26 Jun 2020 09:22:25: 17000000 INFO @ Fri, 26 Jun 2020 09:22:30: 24000000 INFO @ Fri, 26 Jun 2020 09:22:30: 12000000 INFO @ Fri, 26 Jun 2020 09:22:31: 18000000 INFO @ Fri, 26 Jun 2020 09:22:35: 25000000 INFO @ Fri, 26 Jun 2020 09:22:36: 13000000 INFO @ Fri, 26 Jun 2020 09:22:37: 19000000 INFO @ Fri, 26 Jun 2020 09:22:41: 26000000 INFO @ Fri, 26 Jun 2020 09:22:42: 14000000 INFO @ Fri, 26 Jun 2020 09:22:42: 20000000 INFO @ Fri, 26 Jun 2020 09:22:46: 27000000 INFO @ Fri, 26 Jun 2020 09:22:48: 15000000 INFO @ Fri, 26 Jun 2020 09:22:48: 21000000 INFO @ Fri, 26 Jun 2020 09:22:52: 28000000 INFO @ Fri, 26 Jun 2020 09:22:54: 16000000 INFO @ Fri, 26 Jun 2020 09:22:54: 22000000 INFO @ Fri, 26 Jun 2020 09:22:57: 29000000 INFO @ Fri, 26 Jun 2020 09:22:59: 23000000 INFO @ Fri, 26 Jun 2020 09:23:00: 17000000 INFO @ Fri, 26 Jun 2020 09:23:03: 30000000 INFO @ Fri, 26 Jun 2020 09:23:05: 24000000 INFO @ Fri, 26 Jun 2020 09:23:06: 18000000 INFO @ Fri, 26 Jun 2020 09:23:09: 31000000 INFO @ Fri, 26 Jun 2020 09:23:11: 25000000 INFO @ Fri, 26 Jun 2020 09:23:12: 19000000 INFO @ Fri, 26 Jun 2020 09:23:14: 32000000 INFO @ Fri, 26 Jun 2020 09:23:17: 26000000 INFO @ Fri, 26 Jun 2020 09:23:18: 20000000 INFO @ Fri, 26 Jun 2020 09:23:20: 33000000 INFO @ Fri, 26 Jun 2020 09:23:22: 27000000 INFO @ Fri, 26 Jun 2020 09:23:24: 21000000 INFO @ Fri, 26 Jun 2020 09:23:25: 34000000 INFO @ Fri, 26 Jun 2020 09:23:28: 28000000 INFO @ Fri, 26 Jun 2020 09:23:29: 22000000 INFO @ Fri, 26 Jun 2020 09:23:31: 35000000 INFO @ Fri, 26 Jun 2020 09:23:34: 29000000 INFO @ Fri, 26 Jun 2020 09:23:35: 23000000 INFO @ Fri, 26 Jun 2020 09:23:36: 36000000 INFO @ Fri, 26 Jun 2020 09:23:40: 30000000 INFO @ Fri, 26 Jun 2020 09:23:41: 24000000 INFO @ Fri, 26 Jun 2020 09:23:42: 37000000 INFO @ Fri, 26 Jun 2020 09:23:45: 31000000 INFO @ Fri, 26 Jun 2020 09:23:47: 25000000 INFO @ Fri, 26 Jun 2020 09:23:48: 38000000 INFO @ Fri, 26 Jun 2020 09:23:51: 32000000 INFO @ Fri, 26 Jun 2020 09:23:53: 26000000 INFO @ Fri, 26 Jun 2020 09:23:53: 39000000 INFO @ Fri, 26 Jun 2020 09:23:57: 33000000 INFO @ Fri, 26 Jun 2020 09:23:59: 27000000 INFO @ Fri, 26 Jun 2020 09:23:59: 40000000 INFO @ Fri, 26 Jun 2020 09:24:03: 34000000 INFO @ Fri, 26 Jun 2020 09:24:05: 41000000 INFO @ Fri, 26 Jun 2020 09:24:05: 28000000 INFO @ Fri, 26 Jun 2020 09:24:08: 35000000 INFO @ Fri, 26 Jun 2020 09:24:09: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 09:24:09: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 09:24:09: #1 total tags in treatment: 41807471 INFO @ Fri, 26 Jun 2020 09:24:09: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:24:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:24:10: #1 tags after filtering in treatment: 41807471 INFO @ Fri, 26 Jun 2020 09:24:10: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 09:24:10: #1 finished! INFO @ Fri, 26 Jun 2020 09:24:10: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:24:10: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:24:11: 29000000 INFO @ Fri, 26 Jun 2020 09:24:13: #2 number of paired peaks: 36 WARNING @ Fri, 26 Jun 2020 09:24:13: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 09:24:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX685468/SRX685468.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX685468/SRX685468.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX685468/SRX685468.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX685468/SRX685468.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 09:24:14: 36000000 INFO @ Fri, 26 Jun 2020 09:24:17: 30000000 INFO @ Fri, 26 Jun 2020 09:24:19: 37000000 INFO @ Fri, 26 Jun 2020 09:24:22: 31000000 INFO @ Fri, 26 Jun 2020 09:24:25: 38000000 INFO @ Fri, 26 Jun 2020 09:24:28: 32000000 INFO @ Fri, 26 Jun 2020 09:24:30: 39000000 INFO @ Fri, 26 Jun 2020 09:24:34: 33000000 INFO @ Fri, 26 Jun 2020 09:24:36: 40000000 INFO @ Fri, 26 Jun 2020 09:24:40: 34000000 INFO @ Fri, 26 Jun 2020 09:24:42: 41000000 INFO @ Fri, 26 Jun 2020 09:24:45: 35000000 INFO @ Fri, 26 Jun 2020 09:24:47: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 09:24:47: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 09:24:47: #1 total tags in treatment: 41807471 INFO @ Fri, 26 Jun 2020 09:24:47: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:24:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:24:47: #1 tags after filtering in treatment: 41807471 INFO @ Fri, 26 Jun 2020 09:24:47: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 09:24:47: #1 finished! INFO @ Fri, 26 Jun 2020 09:24:47: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:24:47: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:24:50: #2 number of paired peaks: 36 WARNING @ Fri, 26 Jun 2020 09:24:50: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 09:24:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX685468/SRX685468.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX685468/SRX685468.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX685468/SRX685468.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX685468/SRX685468.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 09:24:51: 36000000 INFO @ Fri, 26 Jun 2020 09:24:56: 37000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 26 Jun 2020 09:25:01: 38000000 INFO @ Fri, 26 Jun 2020 09:25:07: 39000000 INFO @ Fri, 26 Jun 2020 09:25:12: 40000000 INFO @ Fri, 26 Jun 2020 09:25:18: 41000000 INFO @ Fri, 26 Jun 2020 09:25:22: #1 tag size is determined as 50 bps INFO @ Fri, 26 Jun 2020 09:25:22: #1 tag size = 50 INFO @ Fri, 26 Jun 2020 09:25:22: #1 total tags in treatment: 41807471 INFO @ Fri, 26 Jun 2020 09:25:22: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:25:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:25:23: #1 tags after filtering in treatment: 41807471 INFO @ Fri, 26 Jun 2020 09:25:23: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 09:25:23: #1 finished! INFO @ Fri, 26 Jun 2020 09:25:23: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:25:23: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:25:26: #2 number of paired peaks: 36 WARNING @ Fri, 26 Jun 2020 09:25:26: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 09:25:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX685468/SRX685468.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX685468/SRX685468.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX685468/SRX685468.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX685468/SRX685468.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。