Job ID = 6528467 SRX = SRX685466 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T15:07:55 prefetch.2.10.7: 1) Downloading 'SRR1556388'... 2020-06-29T15:07:55 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:16:51 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:16:51 prefetch.2.10.7: 1) 'SRR1556388' was downloaded successfully Read 45263312 spots for SRR1556388/SRR1556388.sra Written 45263312 spots for SRR1556388/SRR1556388.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:18:38 45263312 reads; of these: 45263312 (100.00%) were unpaired; of these: 1116991 (2.47%) aligned 0 times 28811244 (63.65%) aligned exactly 1 time 15335077 (33.88%) aligned >1 times 97.53% overall alignment rate Time searching: 00:18:38 Overall time: 00:18:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8275643 / 44146321 = 0.1875 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:59:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX685466/SRX685466.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX685466/SRX685466.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX685466/SRX685466.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX685466/SRX685466.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:59:00: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:59:00: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:59:08: 1000000 INFO @ Tue, 30 Jun 2020 00:59:15: 2000000 INFO @ Tue, 30 Jun 2020 00:59:22: 3000000 INFO @ Tue, 30 Jun 2020 00:59:28: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:59:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX685466/SRX685466.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX685466/SRX685466.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX685466/SRX685466.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX685466/SRX685466.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:59:31: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:59:31: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:59:36: 5000000 INFO @ Tue, 30 Jun 2020 00:59:38: 1000000 INFO @ Tue, 30 Jun 2020 00:59:43: 6000000 INFO @ Tue, 30 Jun 2020 00:59:46: 2000000 INFO @ Tue, 30 Jun 2020 00:59:50: 7000000 INFO @ Tue, 30 Jun 2020 00:59:53: 3000000 INFO @ Tue, 30 Jun 2020 00:59:58: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:00:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX685466/SRX685466.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX685466/SRX685466.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX685466/SRX685466.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX685466/SRX685466.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:00:00: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:00:00: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:00:01: 4000000 INFO @ Tue, 30 Jun 2020 01:00:05: 9000000 INFO @ Tue, 30 Jun 2020 01:00:08: 1000000 INFO @ Tue, 30 Jun 2020 01:00:08: 5000000 INFO @ Tue, 30 Jun 2020 01:00:13: 10000000 INFO @ Tue, 30 Jun 2020 01:00:16: 2000000 INFO @ Tue, 30 Jun 2020 01:00:16: 6000000 INFO @ Tue, 30 Jun 2020 01:00:20: 11000000 INFO @ Tue, 30 Jun 2020 01:00:23: 3000000 INFO @ Tue, 30 Jun 2020 01:00:24: 7000000 INFO @ Tue, 30 Jun 2020 01:00:28: 12000000 INFO @ Tue, 30 Jun 2020 01:00:31: 4000000 INFO @ Tue, 30 Jun 2020 01:00:31: 8000000 INFO @ Tue, 30 Jun 2020 01:00:36: 13000000 INFO @ Tue, 30 Jun 2020 01:00:38: 5000000 INFO @ Tue, 30 Jun 2020 01:00:39: 9000000 INFO @ Tue, 30 Jun 2020 01:00:44: 14000000 INFO @ Tue, 30 Jun 2020 01:00:46: 6000000 INFO @ Tue, 30 Jun 2020 01:00:47: 10000000 INFO @ Tue, 30 Jun 2020 01:00:51: 15000000 INFO @ Tue, 30 Jun 2020 01:00:53: 7000000 INFO @ Tue, 30 Jun 2020 01:00:54: 11000000 INFO @ Tue, 30 Jun 2020 01:00:58: 16000000 INFO @ Tue, 30 Jun 2020 01:01:00: 8000000 INFO @ Tue, 30 Jun 2020 01:01:02: 12000000 INFO @ Tue, 30 Jun 2020 01:01:06: 17000000 INFO @ Tue, 30 Jun 2020 01:01:08: 9000000 INFO @ Tue, 30 Jun 2020 01:01:09: 13000000 INFO @ Tue, 30 Jun 2020 01:01:13: 18000000 INFO @ Tue, 30 Jun 2020 01:01:15: 10000000 INFO @ Tue, 30 Jun 2020 01:01:16: 14000000 INFO @ Tue, 30 Jun 2020 01:01:21: 19000000 INFO @ Tue, 30 Jun 2020 01:01:22: 11000000 INFO @ Tue, 30 Jun 2020 01:01:23: 15000000 INFO @ Tue, 30 Jun 2020 01:01:29: 20000000 INFO @ Tue, 30 Jun 2020 01:01:29: 12000000 INFO @ Tue, 30 Jun 2020 01:01:31: 16000000 INFO @ Tue, 30 Jun 2020 01:01:36: 13000000 INFO @ Tue, 30 Jun 2020 01:01:37: 21000000 INFO @ Tue, 30 Jun 2020 01:01:38: 17000000 INFO @ Tue, 30 Jun 2020 01:01:44: 14000000 INFO @ Tue, 30 Jun 2020 01:01:45: 22000000 INFO @ Tue, 30 Jun 2020 01:01:46: 18000000 INFO @ Tue, 30 Jun 2020 01:01:51: 15000000 INFO @ Tue, 30 Jun 2020 01:01:53: 23000000 INFO @ Tue, 30 Jun 2020 01:01:55: 19000000 INFO @ Tue, 30 Jun 2020 01:01:58: 16000000 INFO @ Tue, 30 Jun 2020 01:02:01: 24000000 INFO @ Tue, 30 Jun 2020 01:02:03: 20000000 INFO @ Tue, 30 Jun 2020 01:02:05: 17000000 INFO @ Tue, 30 Jun 2020 01:02:09: 25000000 INFO @ Tue, 30 Jun 2020 01:02:12: 21000000 INFO @ Tue, 30 Jun 2020 01:02:13: 18000000 INFO @ Tue, 30 Jun 2020 01:02:17: 26000000 INFO @ Tue, 30 Jun 2020 01:02:19: 22000000 INFO @ Tue, 30 Jun 2020 01:02:20: 19000000 INFO @ Tue, 30 Jun 2020 01:02:25: 27000000 INFO @ Tue, 30 Jun 2020 01:02:27: 23000000 INFO @ Tue, 30 Jun 2020 01:02:28: 20000000 INFO @ Tue, 30 Jun 2020 01:02:33: 28000000 INFO @ Tue, 30 Jun 2020 01:02:34: 24000000 INFO @ Tue, 30 Jun 2020 01:02:36: 21000000 INFO @ Tue, 30 Jun 2020 01:02:41: 29000000 INFO @ Tue, 30 Jun 2020 01:02:42: 25000000 INFO @ Tue, 30 Jun 2020 01:02:44: 22000000 INFO @ Tue, 30 Jun 2020 01:02:49: 30000000 INFO @ Tue, 30 Jun 2020 01:02:49: 26000000 INFO @ Tue, 30 Jun 2020 01:02:51: 23000000 INFO @ Tue, 30 Jun 2020 01:02:57: 31000000 INFO @ Tue, 30 Jun 2020 01:02:57: 27000000 INFO @ Tue, 30 Jun 2020 01:02:59: 24000000 INFO @ Tue, 30 Jun 2020 01:03:04: 32000000 INFO @ Tue, 30 Jun 2020 01:03:05: 28000000 INFO @ Tue, 30 Jun 2020 01:03:06: 25000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 01:03:12: 33000000 INFO @ Tue, 30 Jun 2020 01:03:12: 29000000 INFO @ Tue, 30 Jun 2020 01:03:14: 26000000 INFO @ Tue, 30 Jun 2020 01:03:20: 34000000 INFO @ Tue, 30 Jun 2020 01:03:20: 30000000 INFO @ Tue, 30 Jun 2020 01:03:22: 27000000 INFO @ Tue, 30 Jun 2020 01:03:28: 35000000 INFO @ Tue, 30 Jun 2020 01:03:28: 31000000 INFO @ Tue, 30 Jun 2020 01:03:30: 28000000 INFO @ Tue, 30 Jun 2020 01:03:34: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 01:03:34: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 01:03:34: #1 total tags in treatment: 35870678 INFO @ Tue, 30 Jun 2020 01:03:34: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:03:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:03:35: #1 tags after filtering in treatment: 35870678 INFO @ Tue, 30 Jun 2020 01:03:35: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:03:35: #1 finished! INFO @ Tue, 30 Jun 2020 01:03:35: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:03:35: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:03:35: 32000000 INFO @ Tue, 30 Jun 2020 01:03:37: #2 number of paired peaks: 65 WARNING @ Tue, 30 Jun 2020 01:03:37: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:03:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX685466/SRX685466.05_peaks.narrowPeak: No such file or directory INFO @ Tue, 30 Jun 2020 01:03:37: 29000000 pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX685466/SRX685466.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX685466/SRX685466.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX685466/SRX685466.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 01:03:43: 33000000 INFO @ Tue, 30 Jun 2020 01:03:45: 30000000 INFO @ Tue, 30 Jun 2020 01:03:51: 34000000 INFO @ Tue, 30 Jun 2020 01:03:53: 31000000 INFO @ Tue, 30 Jun 2020 01:03:58: 35000000 INFO @ Tue, 30 Jun 2020 01:04:00: 32000000 INFO @ Tue, 30 Jun 2020 01:04:05: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 01:04:05: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 01:04:05: #1 total tags in treatment: 35870678 INFO @ Tue, 30 Jun 2020 01:04:05: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:04:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:04:05: #1 tags after filtering in treatment: 35870678 INFO @ Tue, 30 Jun 2020 01:04:05: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:04:05: #1 finished! INFO @ Tue, 30 Jun 2020 01:04:05: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:04:05: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:04:08: #2 number of paired peaks: 65 WARNING @ Tue, 30 Jun 2020 01:04:08: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:04:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX685466/SRX685466.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX685466/SRX685466.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX685466/SRX685466.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX685466/SRX685466.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 01:04:08: 33000000 INFO @ Tue, 30 Jun 2020 01:04:16: 34000000 INFO @ Tue, 30 Jun 2020 01:04:23: 35000000 INFO @ Tue, 30 Jun 2020 01:04:29: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 01:04:29: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 01:04:29: #1 total tags in treatment: 35870678 INFO @ Tue, 30 Jun 2020 01:04:29: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:04:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:04:30: #1 tags after filtering in treatment: 35870678 INFO @ Tue, 30 Jun 2020 01:04:30: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:04:30: #1 finished! INFO @ Tue, 30 Jun 2020 01:04:30: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:04:30: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:04:32: #2 number of paired peaks: 65 WARNING @ Tue, 30 Jun 2020 01:04:32: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:04:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX685466/SRX685466.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX685466/SRX685466.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX685466/SRX685466.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX685466/SRX685466.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。