Job ID = 6528464 SRX = SRX6827253 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:48:54 prefetch.2.10.7: 1) Downloading 'SRR10094666'... 2020-06-29T14:48:54 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:52:32 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:52:32 prefetch.2.10.7: 1) 'SRR10094666' was downloaded successfully Read 52274882 spots for SRR10094666/SRR10094666.sra Written 52274882 spots for SRR10094666/SRR10094666.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:27 52274882 reads; of these: 52274882 (100.00%) were unpaired; of these: 25378973 (48.55%) aligned 0 times 20698385 (39.60%) aligned exactly 1 time 6197524 (11.86%) aligned >1 times 51.45% overall alignment rate Time searching: 00:11:27 Overall time: 00:11:27 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 3781808 / 26895909 = 0.1406 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:25:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6827253/SRX6827253.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6827253/SRX6827253.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6827253/SRX6827253.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6827253/SRX6827253.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:25:14: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:25:14: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:25:21: 1000000 INFO @ Tue, 30 Jun 2020 00:25:27: 2000000 INFO @ Tue, 30 Jun 2020 00:25:34: 3000000 INFO @ Tue, 30 Jun 2020 00:25:41: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:25:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6827253/SRX6827253.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6827253/SRX6827253.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6827253/SRX6827253.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6827253/SRX6827253.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:25:44: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:25:44: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:25:49: 5000000 INFO @ Tue, 30 Jun 2020 00:25:51: 1000000 INFO @ Tue, 30 Jun 2020 00:25:56: 6000000 INFO @ Tue, 30 Jun 2020 00:25:59: 2000000 INFO @ Tue, 30 Jun 2020 00:26:04: 7000000 INFO @ Tue, 30 Jun 2020 00:26:07: 3000000 INFO @ Tue, 30 Jun 2020 00:26:11: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:26:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6827253/SRX6827253.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6827253/SRX6827253.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6827253/SRX6827253.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6827253/SRX6827253.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:26:14: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:26:14: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:26:15: 4000000 INFO @ Tue, 30 Jun 2020 00:26:19: 9000000 INFO @ Tue, 30 Jun 2020 00:26:22: 1000000 INFO @ Tue, 30 Jun 2020 00:26:23: 5000000 INFO @ Tue, 30 Jun 2020 00:26:27: 10000000 INFO @ Tue, 30 Jun 2020 00:26:30: 2000000 INFO @ Tue, 30 Jun 2020 00:26:31: 6000000 INFO @ Tue, 30 Jun 2020 00:26:34: 11000000 INFO @ Tue, 30 Jun 2020 00:26:38: 3000000 INFO @ Tue, 30 Jun 2020 00:26:39: 7000000 INFO @ Tue, 30 Jun 2020 00:26:42: 12000000 INFO @ Tue, 30 Jun 2020 00:26:46: 4000000 INFO @ Tue, 30 Jun 2020 00:26:47: 8000000 INFO @ Tue, 30 Jun 2020 00:26:50: 13000000 INFO @ Tue, 30 Jun 2020 00:26:54: 5000000 INFO @ Tue, 30 Jun 2020 00:26:55: 9000000 INFO @ Tue, 30 Jun 2020 00:26:57: 14000000 INFO @ Tue, 30 Jun 2020 00:27:02: 6000000 INFO @ Tue, 30 Jun 2020 00:27:03: 10000000 INFO @ Tue, 30 Jun 2020 00:27:05: 15000000 INFO @ Tue, 30 Jun 2020 00:27:11: 7000000 INFO @ Tue, 30 Jun 2020 00:27:11: 11000000 INFO @ Tue, 30 Jun 2020 00:27:13: 16000000 INFO @ Tue, 30 Jun 2020 00:27:19: 8000000 INFO @ Tue, 30 Jun 2020 00:27:19: 12000000 INFO @ Tue, 30 Jun 2020 00:27:20: 17000000 INFO @ Tue, 30 Jun 2020 00:27:27: 13000000 INFO @ Tue, 30 Jun 2020 00:27:27: 9000000 INFO @ Tue, 30 Jun 2020 00:27:28: 18000000 INFO @ Tue, 30 Jun 2020 00:27:35: 14000000 INFO @ Tue, 30 Jun 2020 00:27:35: 10000000 INFO @ Tue, 30 Jun 2020 00:27:36: 19000000 INFO @ Tue, 30 Jun 2020 00:27:43: 15000000 INFO @ Tue, 30 Jun 2020 00:27:43: 11000000 INFO @ Tue, 30 Jun 2020 00:27:44: 20000000 INFO @ Tue, 30 Jun 2020 00:27:51: 16000000 INFO @ Tue, 30 Jun 2020 00:27:52: 12000000 INFO @ Tue, 30 Jun 2020 00:27:52: 21000000 INFO @ Tue, 30 Jun 2020 00:27:59: 17000000 INFO @ Tue, 30 Jun 2020 00:28:00: 22000000 INFO @ Tue, 30 Jun 2020 00:28:00: 13000000 INFO @ Tue, 30 Jun 2020 00:28:07: 23000000 INFO @ Tue, 30 Jun 2020 00:28:07: 18000000 INFO @ Tue, 30 Jun 2020 00:28:08: 14000000 INFO @ Tue, 30 Jun 2020 00:28:08: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:28:08: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:28:08: #1 total tags in treatment: 23114101 INFO @ Tue, 30 Jun 2020 00:28:08: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:28:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:28:09: #1 tags after filtering in treatment: 23114101 INFO @ Tue, 30 Jun 2020 00:28:09: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:28:09: #1 finished! INFO @ Tue, 30 Jun 2020 00:28:09: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:28:09: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:28:10: #2 number of paired peaks: 52 WARNING @ Tue, 30 Jun 2020 00:28:10: Too few paired peaks (52) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:28:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6827253/SRX6827253.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6827253/SRX6827253.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6827253/SRX6827253.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6827253/SRX6827253.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:28:15: 19000000 INFO @ Tue, 30 Jun 2020 00:28:16: 15000000 INFO @ Tue, 30 Jun 2020 00:28:23: 20000000 INFO @ Tue, 30 Jun 2020 00:28:24: 16000000 INFO @ Tue, 30 Jun 2020 00:28:31: 21000000 INFO @ Tue, 30 Jun 2020 00:28:32: 17000000 INFO @ Tue, 30 Jun 2020 00:28:39: 22000000 INFO @ Tue, 30 Jun 2020 00:28:41: 18000000 INFO @ Tue, 30 Jun 2020 00:28:47: 23000000 INFO @ Tue, 30 Jun 2020 00:28:48: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:28:48: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:28:48: #1 total tags in treatment: 23114101 INFO @ Tue, 30 Jun 2020 00:28:48: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:28:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:28:48: #1 tags after filtering in treatment: 23114101 INFO @ Tue, 30 Jun 2020 00:28:48: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:28:48: #1 finished! INFO @ Tue, 30 Jun 2020 00:28:48: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:28:48: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:28:49: 19000000 INFO @ Tue, 30 Jun 2020 00:28:50: #2 number of paired peaks: 52 WARNING @ Tue, 30 Jun 2020 00:28:50: Too few paired peaks (52) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:28:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6827253/SRX6827253.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6827253/SRX6827253.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6827253/SRX6827253.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6827253/SRX6827253.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:28:56: 20000000 BigWig に変換しました。 INFO @ Tue, 30 Jun 2020 00:29:04: 21000000 INFO @ Tue, 30 Jun 2020 00:29:11: 22000000 INFO @ Tue, 30 Jun 2020 00:29:19: 23000000 INFO @ Tue, 30 Jun 2020 00:29:20: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:29:20: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:29:20: #1 total tags in treatment: 23114101 INFO @ Tue, 30 Jun 2020 00:29:20: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:29:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:29:20: #1 tags after filtering in treatment: 23114101 INFO @ Tue, 30 Jun 2020 00:29:20: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:29:20: #1 finished! INFO @ Tue, 30 Jun 2020 00:29:20: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:29:20: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:29:22: #2 number of paired peaks: 52 WARNING @ Tue, 30 Jun 2020 00:29:22: Too few paired peaks (52) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:29:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6827253/SRX6827253.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6827253/SRX6827253.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6827253/SRX6827253.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6827253/SRX6827253.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling