Job ID = 4303130 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 40,755,850 reads read : 40,755,850 reads written : 40,755,850 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR1552296.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:00 40755850 reads; of these: 40755850 (100.00%) were unpaired; of these: 2750228 (6.75%) aligned 0 times 33284248 (81.67%) aligned exactly 1 time 4721374 (11.58%) aligned >1 times 93.25% overall alignment rate Time searching: 00:08:00 Overall time: 00:08:00 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 5884505 / 38005622 = 0.1548 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Thu, 12 Dec 2019 01:05:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX681827/SRX681827.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX681827/SRX681827.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX681827/SRX681827.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX681827/SRX681827.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 01:05:37: #1 read tag files... INFO @ Thu, 12 Dec 2019 01:05:37: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 01:05:42: 1000000 INFO @ Thu, 12 Dec 2019 01:05:46: 2000000 INFO @ Thu, 12 Dec 2019 01:05:51: 3000000 INFO @ Thu, 12 Dec 2019 01:05:56: 4000000 INFO @ Thu, 12 Dec 2019 01:06:00: 5000000 INFO @ Thu, 12 Dec 2019 01:06:05: 6000000 INFO @ Thu, 12 Dec 2019 01:06:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX681827/SRX681827.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX681827/SRX681827.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX681827/SRX681827.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX681827/SRX681827.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 01:06:07: #1 read tag files... INFO @ Thu, 12 Dec 2019 01:06:07: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 01:06:10: 7000000 INFO @ Thu, 12 Dec 2019 01:06:12: 1000000 INFO @ Thu, 12 Dec 2019 01:06:14: 8000000 INFO @ Thu, 12 Dec 2019 01:06:17: 2000000 INFO @ Thu, 12 Dec 2019 01:06:19: 9000000 INFO @ Thu, 12 Dec 2019 01:06:21: 3000000 INFO @ Thu, 12 Dec 2019 01:06:23: 10000000 INFO @ Thu, 12 Dec 2019 01:06:26: 4000000 INFO @ Thu, 12 Dec 2019 01:06:28: 11000000 INFO @ Thu, 12 Dec 2019 01:06:31: 5000000 INFO @ Thu, 12 Dec 2019 01:06:33: 12000000 BedGraph に変換中... INFO @ Thu, 12 Dec 2019 01:06:36: 6000000 INFO @ Thu, 12 Dec 2019 01:06:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX681827/SRX681827.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX681827/SRX681827.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX681827/SRX681827.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX681827/SRX681827.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 01:06:37: #1 read tag files... INFO @ Thu, 12 Dec 2019 01:06:37: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 01:06:37: 13000000 INFO @ Thu, 12 Dec 2019 01:06:40: 7000000 INFO @ Thu, 12 Dec 2019 01:06:42: 1000000 INFO @ Thu, 12 Dec 2019 01:06:42: 14000000 INFO @ Thu, 12 Dec 2019 01:06:45: 8000000 INFO @ Thu, 12 Dec 2019 01:06:47: 2000000 INFO @ Thu, 12 Dec 2019 01:06:47: 15000000 INFO @ Thu, 12 Dec 2019 01:06:50: 9000000 INFO @ Thu, 12 Dec 2019 01:06:52: 3000000 INFO @ Thu, 12 Dec 2019 01:06:52: 16000000 INFO @ Thu, 12 Dec 2019 01:06:55: 10000000 INFO @ Thu, 12 Dec 2019 01:06:57: 4000000 INFO @ Thu, 12 Dec 2019 01:06:57: 17000000 INFO @ Thu, 12 Dec 2019 01:06:59: 11000000 INFO @ Thu, 12 Dec 2019 01:07:02: 5000000 INFO @ Thu, 12 Dec 2019 01:07:02: 18000000 INFO @ Thu, 12 Dec 2019 01:07:04: 12000000 INFO @ Thu, 12 Dec 2019 01:07:07: 6000000 INFO @ Thu, 12 Dec 2019 01:07:08: 19000000 INFO @ Thu, 12 Dec 2019 01:07:09: 13000000 INFO @ Thu, 12 Dec 2019 01:07:12: 7000000 INFO @ Thu, 12 Dec 2019 01:07:12: 20000000 INFO @ Thu, 12 Dec 2019 01:07:14: 14000000 INFO @ Thu, 12 Dec 2019 01:07:17: 8000000 INFO @ Thu, 12 Dec 2019 01:07:17: 21000000 INFO @ Thu, 12 Dec 2019 01:07:19: 15000000 INFO @ Thu, 12 Dec 2019 01:07:22: 9000000 INFO @ Thu, 12 Dec 2019 01:07:22: 22000000 INFO @ Thu, 12 Dec 2019 01:07:23: 16000000 INFO @ Thu, 12 Dec 2019 01:07:27: 10000000 INFO @ Thu, 12 Dec 2019 01:07:27: 23000000 INFO @ Thu, 12 Dec 2019 01:07:28: 17000000 INFO @ Thu, 12 Dec 2019 01:07:32: 11000000 INFO @ Thu, 12 Dec 2019 01:07:32: 24000000 INFO @ Thu, 12 Dec 2019 01:07:33: 18000000 INFO @ Thu, 12 Dec 2019 01:07:37: 12000000 INFO @ Thu, 12 Dec 2019 01:07:37: 25000000 INFO @ Thu, 12 Dec 2019 01:07:37: 19000000 INFO @ Thu, 12 Dec 2019 01:07:42: 13000000 INFO @ Thu, 12 Dec 2019 01:07:42: 20000000 INFO @ Thu, 12 Dec 2019 01:07:42: 26000000 INFO @ Thu, 12 Dec 2019 01:07:47: 21000000 INFO @ Thu, 12 Dec 2019 01:07:47: 14000000 INFO @ Thu, 12 Dec 2019 01:07:47: 27000000 INFO @ Thu, 12 Dec 2019 01:07:52: 22000000 INFO @ Thu, 12 Dec 2019 01:07:52: 15000000 INFO @ Thu, 12 Dec 2019 01:07:52: 28000000 INFO @ Thu, 12 Dec 2019 01:07:56: 23000000 INFO @ Thu, 12 Dec 2019 01:07:57: 16000000 INFO @ Thu, 12 Dec 2019 01:07:57: 29000000 INFO @ Thu, 12 Dec 2019 01:08:01: 24000000 INFO @ Thu, 12 Dec 2019 01:08:02: 17000000 INFO @ Thu, 12 Dec 2019 01:08:02: 30000000 INFO @ Thu, 12 Dec 2019 01:08:06: 25000000 INFO @ Thu, 12 Dec 2019 01:08:07: 18000000 INFO @ Thu, 12 Dec 2019 01:08:07: 31000000 INFO @ Thu, 12 Dec 2019 01:08:11: 26000000 INFO @ Thu, 12 Dec 2019 01:08:12: 19000000 INFO @ Thu, 12 Dec 2019 01:08:12: 32000000 INFO @ Thu, 12 Dec 2019 01:08:13: #1 tag size is determined as 50 bps INFO @ Thu, 12 Dec 2019 01:08:13: #1 tag size = 50 INFO @ Thu, 12 Dec 2019 01:08:13: #1 total tags in treatment: 32121117 INFO @ Thu, 12 Dec 2019 01:08:13: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 01:08:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 01:08:13: #1 tags after filtering in treatment: 32121117 INFO @ Thu, 12 Dec 2019 01:08:13: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 01:08:13: #1 finished! INFO @ Thu, 12 Dec 2019 01:08:13: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 01:08:13: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 01:08:15: #2 number of paired peaks: 11 WARNING @ Thu, 12 Dec 2019 01:08:15: Too few paired peaks (11) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 01:08:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX681827/SRX681827.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX681827/SRX681827.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX681827/SRX681827.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX681827/SRX681827.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 12 Dec 2019 01:08:15: 27000000 INFO @ Thu, 12 Dec 2019 01:08:17: 20000000 INFO @ Thu, 12 Dec 2019 01:08:20: 28000000 INFO @ Thu, 12 Dec 2019 01:08:21: 21000000 INFO @ Thu, 12 Dec 2019 01:08:25: 29000000 INFO @ Thu, 12 Dec 2019 01:08:26: 22000000 INFO @ Thu, 12 Dec 2019 01:08:30: 30000000 INFO @ Thu, 12 Dec 2019 01:08:30: 23000000 INFO @ Thu, 12 Dec 2019 01:08:35: 31000000 INFO @ Thu, 12 Dec 2019 01:08:35: 24000000 INFO @ Thu, 12 Dec 2019 01:08:40: 25000000 INFO @ Thu, 12 Dec 2019 01:08:40: 32000000 INFO @ Thu, 12 Dec 2019 01:08:41: #1 tag size is determined as 50 bps INFO @ Thu, 12 Dec 2019 01:08:41: #1 tag size = 50 INFO @ Thu, 12 Dec 2019 01:08:41: #1 total tags in treatment: 32121117 INFO @ Thu, 12 Dec 2019 01:08:41: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 01:08:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 01:08:41: #1 tags after filtering in treatment: 32121117 INFO @ Thu, 12 Dec 2019 01:08:41: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 01:08:41: #1 finished! INFO @ Thu, 12 Dec 2019 01:08:41: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 01:08:41: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 01:08:43: #2 number of paired peaks: 11 WARNING @ Thu, 12 Dec 2019 01:08:43: Too few paired peaks (11) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 01:08:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX681827/SRX681827.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX681827/SRX681827.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX681827/SRX681827.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX681827/SRX681827.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 12 Dec 2019 01:08:44: 26000000 INFO @ Thu, 12 Dec 2019 01:08:49: 27000000 INFO @ Thu, 12 Dec 2019 01:08:54: 28000000 INFO @ Thu, 12 Dec 2019 01:08:58: 29000000 INFO @ Thu, 12 Dec 2019 01:09:03: 30000000 INFO @ Thu, 12 Dec 2019 01:09:07: 31000000 INFO @ Thu, 12 Dec 2019 01:09:12: 32000000 INFO @ Thu, 12 Dec 2019 01:09:12: #1 tag size is determined as 50 bps INFO @ Thu, 12 Dec 2019 01:09:12: #1 tag size = 50 INFO @ Thu, 12 Dec 2019 01:09:12: #1 total tags in treatment: 32121117 INFO @ Thu, 12 Dec 2019 01:09:12: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 01:09:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 01:09:13: #1 tags after filtering in treatment: 32121117 INFO @ Thu, 12 Dec 2019 01:09:13: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 01:09:13: #1 finished! INFO @ Thu, 12 Dec 2019 01:09:13: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 01:09:13: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 01:09:14: #2 number of paired peaks: 11 WARNING @ Thu, 12 Dec 2019 01:09:14: Too few paired peaks (11) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 01:09:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX681827/SRX681827.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX681827/SRX681827.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX681827/SRX681827.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX681827/SRX681827.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。