Job ID = 4303124 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 46,393,557 reads read : 46,393,557 reads written : 46,393,557 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR1552290.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:55 46393557 reads; of these: 46393557 (100.00%) were unpaired; of these: 4868690 (10.49%) aligned 0 times 35810408 (77.19%) aligned exactly 1 time 5714459 (12.32%) aligned >1 times 89.51% overall alignment rate Time searching: 00:12:55 Overall time: 00:12:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 7603749 / 41524867 = 0.1831 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Thu, 12 Dec 2019 01:13:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX681821/SRX681821.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX681821/SRX681821.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX681821/SRX681821.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX681821/SRX681821.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 01:13:56: #1 read tag files... INFO @ Thu, 12 Dec 2019 01:13:56: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 01:14:05: 1000000 INFO @ Thu, 12 Dec 2019 01:14:12: 2000000 INFO @ Thu, 12 Dec 2019 01:14:20: 3000000 INFO @ Thu, 12 Dec 2019 01:14:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX681821/SRX681821.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX681821/SRX681821.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX681821/SRX681821.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX681821/SRX681821.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 01:14:25: #1 read tag files... INFO @ Thu, 12 Dec 2019 01:14:25: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 01:14:28: 4000000 INFO @ Thu, 12 Dec 2019 01:14:36: 1000000 INFO @ Thu, 12 Dec 2019 01:14:37: 5000000 INFO @ Thu, 12 Dec 2019 01:14:47: 6000000 INFO @ Thu, 12 Dec 2019 01:14:47: 2000000 BedGraph に変換中... INFO @ Thu, 12 Dec 2019 01:14:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX681821/SRX681821.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX681821/SRX681821.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX681821/SRX681821.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX681821/SRX681821.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 12 Dec 2019 01:14:55: #1 read tag files... INFO @ Thu, 12 Dec 2019 01:14:55: #1 read treatment tags... INFO @ Thu, 12 Dec 2019 01:14:57: 7000000 INFO @ Thu, 12 Dec 2019 01:14:57: 3000000 INFO @ Thu, 12 Dec 2019 01:15:07: 8000000 INFO @ Thu, 12 Dec 2019 01:15:08: 4000000 INFO @ Thu, 12 Dec 2019 01:15:08: 1000000 INFO @ Thu, 12 Dec 2019 01:15:17: 9000000 INFO @ Thu, 12 Dec 2019 01:15:19: 5000000 INFO @ Thu, 12 Dec 2019 01:15:20: 2000000 INFO @ Thu, 12 Dec 2019 01:15:28: 10000000 INFO @ Thu, 12 Dec 2019 01:15:29: 6000000 INFO @ Thu, 12 Dec 2019 01:15:31: 3000000 INFO @ Thu, 12 Dec 2019 01:15:39: 11000000 INFO @ Thu, 12 Dec 2019 01:15:39: 7000000 INFO @ Thu, 12 Dec 2019 01:15:44: 4000000 INFO @ Thu, 12 Dec 2019 01:15:49: 8000000 INFO @ Thu, 12 Dec 2019 01:15:50: 12000000 INFO @ Thu, 12 Dec 2019 01:15:57: 5000000 INFO @ Thu, 12 Dec 2019 01:16:00: 9000000 INFO @ Thu, 12 Dec 2019 01:16:00: 13000000 INFO @ Thu, 12 Dec 2019 01:16:09: 6000000 INFO @ Thu, 12 Dec 2019 01:16:11: 14000000 INFO @ Thu, 12 Dec 2019 01:16:11: 10000000 INFO @ Thu, 12 Dec 2019 01:16:20: 7000000 INFO @ Thu, 12 Dec 2019 01:16:21: 15000000 INFO @ Thu, 12 Dec 2019 01:16:22: 11000000 INFO @ Thu, 12 Dec 2019 01:16:32: 16000000 INFO @ Thu, 12 Dec 2019 01:16:32: 8000000 INFO @ Thu, 12 Dec 2019 01:16:33: 12000000 INFO @ Thu, 12 Dec 2019 01:16:42: 17000000 INFO @ Thu, 12 Dec 2019 01:16:44: 13000000 INFO @ Thu, 12 Dec 2019 01:16:44: 9000000 INFO @ Thu, 12 Dec 2019 01:16:52: 18000000 INFO @ Thu, 12 Dec 2019 01:16:55: 14000000 INFO @ Thu, 12 Dec 2019 01:16:56: 10000000 INFO @ Thu, 12 Dec 2019 01:17:02: 19000000 INFO @ Thu, 12 Dec 2019 01:17:05: 15000000 INFO @ Thu, 12 Dec 2019 01:17:09: 11000000 INFO @ Thu, 12 Dec 2019 01:17:12: 20000000 INFO @ Thu, 12 Dec 2019 01:17:15: 16000000 INFO @ Thu, 12 Dec 2019 01:17:22: 12000000 INFO @ Thu, 12 Dec 2019 01:17:23: 21000000 INFO @ Thu, 12 Dec 2019 01:17:26: 17000000 INFO @ Thu, 12 Dec 2019 01:17:33: 22000000 INFO @ Thu, 12 Dec 2019 01:17:35: 13000000 INFO @ Thu, 12 Dec 2019 01:17:36: 18000000 INFO @ Thu, 12 Dec 2019 01:17:43: 23000000 INFO @ Thu, 12 Dec 2019 01:17:46: 19000000 INFO @ Thu, 12 Dec 2019 01:17:48: 14000000 INFO @ Thu, 12 Dec 2019 01:17:53: 24000000 INFO @ Thu, 12 Dec 2019 01:17:56: 20000000 INFO @ Thu, 12 Dec 2019 01:18:00: 15000000 INFO @ Thu, 12 Dec 2019 01:18:03: 25000000 INFO @ Thu, 12 Dec 2019 01:18:06: 21000000 INFO @ Thu, 12 Dec 2019 01:18:12: 16000000 INFO @ Thu, 12 Dec 2019 01:18:14: 26000000 INFO @ Thu, 12 Dec 2019 01:18:16: 22000000 INFO @ Thu, 12 Dec 2019 01:18:24: 17000000 INFO @ Thu, 12 Dec 2019 01:18:25: 27000000 INFO @ Thu, 12 Dec 2019 01:18:27: 23000000 INFO @ Thu, 12 Dec 2019 01:18:35: 28000000 INFO @ Thu, 12 Dec 2019 01:18:36: 18000000 INFO @ Thu, 12 Dec 2019 01:18:37: 24000000 INFO @ Thu, 12 Dec 2019 01:18:45: 29000000 INFO @ Thu, 12 Dec 2019 01:18:48: 19000000 INFO @ Thu, 12 Dec 2019 01:18:48: 25000000 INFO @ Thu, 12 Dec 2019 01:18:56: 30000000 INFO @ Thu, 12 Dec 2019 01:18:59: 20000000 INFO @ Thu, 12 Dec 2019 01:19:00: 26000000 INFO @ Thu, 12 Dec 2019 01:19:06: 31000000 INFO @ Thu, 12 Dec 2019 01:19:11: 21000000 INFO @ Thu, 12 Dec 2019 01:19:11: 27000000 INFO @ Thu, 12 Dec 2019 01:19:16: 32000000 INFO @ Thu, 12 Dec 2019 01:19:22: 28000000 INFO @ Thu, 12 Dec 2019 01:19:22: 22000000 INFO @ Thu, 12 Dec 2019 01:19:26: 33000000 INFO @ Thu, 12 Dec 2019 01:19:33: 29000000 INFO @ Thu, 12 Dec 2019 01:19:34: 23000000 INFO @ Thu, 12 Dec 2019 01:19:36: #1 tag size is determined as 50 bps INFO @ Thu, 12 Dec 2019 01:19:36: #1 tag size = 50 INFO @ Thu, 12 Dec 2019 01:19:36: #1 total tags in treatment: 33921118 INFO @ Thu, 12 Dec 2019 01:19:36: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 01:19:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 01:19:37: #1 tags after filtering in treatment: 33921118 INFO @ Thu, 12 Dec 2019 01:19:37: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 01:19:37: #1 finished! INFO @ Thu, 12 Dec 2019 01:19:37: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 01:19:37: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 01:19:40: #2 number of paired peaks: 38 WARNING @ Thu, 12 Dec 2019 01:19:40: Too few paired peaks (38) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 01:19:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX681821/SRX681821.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX681821/SRX681821.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX681821/SRX681821.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX681821/SRX681821.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 12 Dec 2019 01:19:43: 30000000 INFO @ Thu, 12 Dec 2019 01:19:45: 24000000 INFO @ Thu, 12 Dec 2019 01:19:53: 31000000 INFO @ Thu, 12 Dec 2019 01:19:56: 25000000 INFO @ Thu, 12 Dec 2019 01:20:04: 32000000 INFO @ Thu, 12 Dec 2019 01:20:09: 26000000 INFO @ Thu, 12 Dec 2019 01:20:14: 33000000 INFO @ Thu, 12 Dec 2019 01:20:20: 27000000 INFO @ Thu, 12 Dec 2019 01:20:24: #1 tag size is determined as 50 bps INFO @ Thu, 12 Dec 2019 01:20:24: #1 tag size = 50 INFO @ Thu, 12 Dec 2019 01:20:24: #1 total tags in treatment: 33921118 INFO @ Thu, 12 Dec 2019 01:20:24: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 01:20:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 01:20:25: #1 tags after filtering in treatment: 33921118 INFO @ Thu, 12 Dec 2019 01:20:25: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 01:20:25: #1 finished! INFO @ Thu, 12 Dec 2019 01:20:25: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 01:20:25: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 01:20:27: #2 number of paired peaks: 38 WARNING @ Thu, 12 Dec 2019 01:20:27: Too few paired peaks (38) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 01:20:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX681821/SRX681821.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX681821/SRX681821.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX681821/SRX681821.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX681821/SRX681821.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 12 Dec 2019 01:20:31: 28000000 INFO @ Thu, 12 Dec 2019 01:20:42: 29000000 INFO @ Thu, 12 Dec 2019 01:20:54: 30000000 INFO @ Thu, 12 Dec 2019 01:21:05: 31000000 INFO @ Thu, 12 Dec 2019 01:21:16: 32000000 INFO @ Thu, 12 Dec 2019 01:21:27: 33000000 INFO @ Thu, 12 Dec 2019 01:21:37: #1 tag size is determined as 50 bps INFO @ Thu, 12 Dec 2019 01:21:37: #1 tag size = 50 INFO @ Thu, 12 Dec 2019 01:21:37: #1 total tags in treatment: 33921118 INFO @ Thu, 12 Dec 2019 01:21:37: #1 user defined the maximum tags... INFO @ Thu, 12 Dec 2019 01:21:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 12 Dec 2019 01:21:38: #1 tags after filtering in treatment: 33921118 INFO @ Thu, 12 Dec 2019 01:21:38: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 12 Dec 2019 01:21:38: #1 finished! INFO @ Thu, 12 Dec 2019 01:21:38: #2 Build Peak Model... INFO @ Thu, 12 Dec 2019 01:21:38: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 12 Dec 2019 01:21:41: #2 number of paired peaks: 38 WARNING @ Thu, 12 Dec 2019 01:21:41: Too few paired peaks (38) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 12 Dec 2019 01:21:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX681821/SRX681821.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX681821/SRX681821.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX681821/SRX681821.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX681821/SRX681821.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。