Job ID = 6528463 SRX = SRX681813 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T15:06:40 prefetch.2.10.7: 1) Downloading 'SRR1552282'... 2020-06-29T15:06:40 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:33:02 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:33:02 prefetch.2.10.7: 1) 'SRR1552282' was downloaded successfully Read 216875939 spots for SRR1552282/SRR1552282.sra Written 216875939 spots for SRR1552282/SRR1552282.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:55:37 216875939 reads; of these: 216875939 (100.00%) were unpaired; of these: 27360129 (12.62%) aligned 0 times 168128861 (77.52%) aligned exactly 1 time 21386949 (9.86%) aligned >1 times 87.38% overall alignment rate Time searching: 00:55:38 Overall time: 00:55:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 80 files... [bam_rmdupse_core] 144193162 / 189515810 = 0.7609 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 03:16:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX681813/SRX681813.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX681813/SRX681813.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX681813/SRX681813.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX681813/SRX681813.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 03:16:32: #1 read tag files... INFO @ Tue, 30 Jun 2020 03:16:32: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 03:16:38: 1000000 INFO @ Tue, 30 Jun 2020 03:16:43: 2000000 INFO @ Tue, 30 Jun 2020 03:16:49: 3000000 INFO @ Tue, 30 Jun 2020 03:16:55: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 03:17:02: 5000000 INFO @ Tue, 30 Jun 2020 03:17:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX681813/SRX681813.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX681813/SRX681813.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX681813/SRX681813.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX681813/SRX681813.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 03:17:02: #1 read tag files... INFO @ Tue, 30 Jun 2020 03:17:02: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 03:17:08: 6000000 INFO @ Tue, 30 Jun 2020 03:17:09: 1000000 INFO @ Tue, 30 Jun 2020 03:17:15: 7000000 INFO @ Tue, 30 Jun 2020 03:17:15: 2000000 INFO @ Tue, 30 Jun 2020 03:17:22: 8000000 INFO @ Tue, 30 Jun 2020 03:17:22: 3000000 INFO @ Tue, 30 Jun 2020 03:17:28: 9000000 INFO @ Tue, 30 Jun 2020 03:17:29: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 03:17:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX681813/SRX681813.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX681813/SRX681813.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX681813/SRX681813.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX681813/SRX681813.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 03:17:32: #1 read tag files... INFO @ Tue, 30 Jun 2020 03:17:32: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 03:17:35: 10000000 INFO @ Tue, 30 Jun 2020 03:17:36: 5000000 INFO @ Tue, 30 Jun 2020 03:17:38: 1000000 INFO @ Tue, 30 Jun 2020 03:17:42: 11000000 INFO @ Tue, 30 Jun 2020 03:17:43: 6000000 INFO @ Tue, 30 Jun 2020 03:17:45: 2000000 INFO @ Tue, 30 Jun 2020 03:17:48: 12000000 INFO @ Tue, 30 Jun 2020 03:17:50: 7000000 INFO @ Tue, 30 Jun 2020 03:17:52: 3000000 INFO @ Tue, 30 Jun 2020 03:17:55: 13000000 INFO @ Tue, 30 Jun 2020 03:17:57: 8000000 INFO @ Tue, 30 Jun 2020 03:17:59: 4000000 INFO @ Tue, 30 Jun 2020 03:18:02: 14000000 INFO @ Tue, 30 Jun 2020 03:18:04: 9000000 INFO @ Tue, 30 Jun 2020 03:18:05: 5000000 INFO @ Tue, 30 Jun 2020 03:18:09: 15000000 INFO @ Tue, 30 Jun 2020 03:18:11: 10000000 INFO @ Tue, 30 Jun 2020 03:18:12: 6000000 INFO @ Tue, 30 Jun 2020 03:18:15: 16000000 INFO @ Tue, 30 Jun 2020 03:18:18: 11000000 INFO @ Tue, 30 Jun 2020 03:18:19: 7000000 INFO @ Tue, 30 Jun 2020 03:18:22: 17000000 INFO @ Tue, 30 Jun 2020 03:18:25: 12000000 INFO @ Tue, 30 Jun 2020 03:18:26: 8000000 INFO @ Tue, 30 Jun 2020 03:18:29: 18000000 INFO @ Tue, 30 Jun 2020 03:18:32: 13000000 INFO @ Tue, 30 Jun 2020 03:18:33: 9000000 INFO @ Tue, 30 Jun 2020 03:18:36: 19000000 INFO @ Tue, 30 Jun 2020 03:18:39: 14000000 INFO @ Tue, 30 Jun 2020 03:18:40: 10000000 INFO @ Tue, 30 Jun 2020 03:18:43: 20000000 INFO @ Tue, 30 Jun 2020 03:18:46: 15000000 INFO @ Tue, 30 Jun 2020 03:18:47: 11000000 INFO @ Tue, 30 Jun 2020 03:18:49: 21000000 INFO @ Tue, 30 Jun 2020 03:18:52: 16000000 INFO @ Tue, 30 Jun 2020 03:18:53: 12000000 INFO @ Tue, 30 Jun 2020 03:18:56: 22000000 INFO @ Tue, 30 Jun 2020 03:18:59: 17000000 INFO @ Tue, 30 Jun 2020 03:19:00: 13000000 INFO @ Tue, 30 Jun 2020 03:19:02: 23000000 INFO @ Tue, 30 Jun 2020 03:19:06: 18000000 INFO @ Tue, 30 Jun 2020 03:19:07: 14000000 INFO @ Tue, 30 Jun 2020 03:19:09: 24000000 INFO @ Tue, 30 Jun 2020 03:19:12: 19000000 INFO @ Tue, 30 Jun 2020 03:19:14: 15000000 INFO @ Tue, 30 Jun 2020 03:19:16: 25000000 INFO @ Tue, 30 Jun 2020 03:19:19: 20000000 INFO @ Tue, 30 Jun 2020 03:19:20: 16000000 INFO @ Tue, 30 Jun 2020 03:19:23: 26000000 INFO @ Tue, 30 Jun 2020 03:19:26: 21000000 INFO @ Tue, 30 Jun 2020 03:19:27: 17000000 INFO @ Tue, 30 Jun 2020 03:19:29: 27000000 INFO @ Tue, 30 Jun 2020 03:19:32: 22000000 INFO @ Tue, 30 Jun 2020 03:19:34: 18000000 INFO @ Tue, 30 Jun 2020 03:19:36: 28000000 INFO @ Tue, 30 Jun 2020 03:19:39: 23000000 INFO @ Tue, 30 Jun 2020 03:19:40: 19000000 INFO @ Tue, 30 Jun 2020 03:19:42: 29000000 INFO @ Tue, 30 Jun 2020 03:19:45: 24000000 INFO @ Tue, 30 Jun 2020 03:19:47: 20000000 INFO @ Tue, 30 Jun 2020 03:19:49: 30000000 INFO @ Tue, 30 Jun 2020 03:19:52: 25000000 INFO @ Tue, 30 Jun 2020 03:19:53: 21000000 INFO @ Tue, 30 Jun 2020 03:19:55: 31000000 INFO @ Tue, 30 Jun 2020 03:19:58: 26000000 INFO @ Tue, 30 Jun 2020 03:20:00: 22000000 INFO @ Tue, 30 Jun 2020 03:20:02: 32000000 INFO @ Tue, 30 Jun 2020 03:20:05: 27000000 INFO @ Tue, 30 Jun 2020 03:20:06: 23000000 INFO @ Tue, 30 Jun 2020 03:20:08: 33000000 INFO @ Tue, 30 Jun 2020 03:20:11: 28000000 INFO @ Tue, 30 Jun 2020 03:20:12: 24000000 INFO @ Tue, 30 Jun 2020 03:20:15: 34000000 INFO @ Tue, 30 Jun 2020 03:20:17: 29000000 INFO @ Tue, 30 Jun 2020 03:20:19: 25000000 INFO @ Tue, 30 Jun 2020 03:20:21: 35000000 INFO @ Tue, 30 Jun 2020 03:20:24: 30000000 INFO @ Tue, 30 Jun 2020 03:20:25: 26000000 INFO @ Tue, 30 Jun 2020 03:20:28: 36000000 INFO @ Tue, 30 Jun 2020 03:20:30: 31000000 INFO @ Tue, 30 Jun 2020 03:20:32: 27000000 INFO @ Tue, 30 Jun 2020 03:20:34: 37000000 INFO @ Tue, 30 Jun 2020 03:20:37: 32000000 INFO @ Tue, 30 Jun 2020 03:20:38: 28000000 INFO @ Tue, 30 Jun 2020 03:20:41: 38000000 INFO @ Tue, 30 Jun 2020 03:20:43: 33000000 INFO @ Tue, 30 Jun 2020 03:20:45: 29000000 INFO @ Tue, 30 Jun 2020 03:20:47: 39000000 INFO @ Tue, 30 Jun 2020 03:20:50: 34000000 INFO @ Tue, 30 Jun 2020 03:20:51: 30000000 INFO @ Tue, 30 Jun 2020 03:20:54: 40000000 INFO @ Tue, 30 Jun 2020 03:20:56: 35000000 INFO @ Tue, 30 Jun 2020 03:20:58: 31000000 INFO @ Tue, 30 Jun 2020 03:21:00: 41000000 INFO @ Tue, 30 Jun 2020 03:21:03: 36000000 INFO @ Tue, 30 Jun 2020 03:21:04: 32000000 INFO @ Tue, 30 Jun 2020 03:21:07: 42000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 03:21:10: 37000000 INFO @ Tue, 30 Jun 2020 03:21:11: 33000000 INFO @ Tue, 30 Jun 2020 03:21:14: 43000000 INFO @ Tue, 30 Jun 2020 03:21:17: 38000000 INFO @ Tue, 30 Jun 2020 03:21:18: 34000000 INFO @ Tue, 30 Jun 2020 03:21:21: 44000000 INFO @ Tue, 30 Jun 2020 03:21:24: 39000000 INFO @ Tue, 30 Jun 2020 03:21:25: 35000000 INFO @ Tue, 30 Jun 2020 03:21:28: 45000000 INFO @ Tue, 30 Jun 2020 03:21:30: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 03:21:30: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 03:21:30: #1 total tags in treatment: 45322648 INFO @ Tue, 30 Jun 2020 03:21:30: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 03:21:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 03:21:31: #1 tags after filtering in treatment: 45322648 INFO @ Tue, 30 Jun 2020 03:21:31: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 03:21:31: #1 finished! INFO @ Tue, 30 Jun 2020 03:21:31: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 03:21:31: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 03:21:31: 40000000 INFO @ Tue, 30 Jun 2020 03:21:32: 36000000 INFO @ Tue, 30 Jun 2020 03:21:34: #2 number of paired peaks: 48 WARNING @ Tue, 30 Jun 2020 03:21:34: Too few paired peaks (48) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 03:21:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX681813/SRX681813.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX681813/SRX681813.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX681813/SRX681813.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX681813/SRX681813.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 03:21:37: 41000000 INFO @ Tue, 30 Jun 2020 03:21:38: 37000000 INFO @ Tue, 30 Jun 2020 03:21:44: 42000000 INFO @ Tue, 30 Jun 2020 03:21:45: 38000000 INFO @ Tue, 30 Jun 2020 03:21:50: 43000000 INFO @ Tue, 30 Jun 2020 03:21:51: 39000000 INFO @ Tue, 30 Jun 2020 03:21:57: 44000000 INFO @ Tue, 30 Jun 2020 03:21:58: 40000000 INFO @ Tue, 30 Jun 2020 03:22:03: 45000000 INFO @ Tue, 30 Jun 2020 03:22:04: 41000000 INFO @ Tue, 30 Jun 2020 03:22:06: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 03:22:06: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 03:22:06: #1 total tags in treatment: 45322648 INFO @ Tue, 30 Jun 2020 03:22:06: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 03:22:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 03:22:06: #1 tags after filtering in treatment: 45322648 INFO @ Tue, 30 Jun 2020 03:22:06: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 03:22:06: #1 finished! INFO @ Tue, 30 Jun 2020 03:22:06: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 03:22:06: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 03:22:09: #2 number of paired peaks: 48 WARNING @ Tue, 30 Jun 2020 03:22:09: Too few paired peaks (48) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 03:22:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX681813/SRX681813.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX681813/SRX681813.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX681813/SRX681813.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX681813/SRX681813.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 03:22:10: 42000000 INFO @ Tue, 30 Jun 2020 03:22:16: 43000000 INFO @ Tue, 30 Jun 2020 03:22:22: 44000000 INFO @ Tue, 30 Jun 2020 03:22:28: 45000000 INFO @ Tue, 30 Jun 2020 03:22:30: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 03:22:30: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 03:22:30: #1 total tags in treatment: 45322648 INFO @ Tue, 30 Jun 2020 03:22:30: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 03:22:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 03:22:31: #1 tags after filtering in treatment: 45322648 INFO @ Tue, 30 Jun 2020 03:22:31: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 03:22:31: #1 finished! INFO @ Tue, 30 Jun 2020 03:22:31: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 03:22:31: #2 looking for paired plus/minus strand peaks... BigWig に変換しました。 INFO @ Tue, 30 Jun 2020 03:22:33: #2 number of paired peaks: 48 WARNING @ Tue, 30 Jun 2020 03:22:33: Too few paired peaks (48) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 03:22:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX681813/SRX681813.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX681813/SRX681813.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX681813/SRX681813.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX681813/SRX681813.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling