Job ID = 6528456 SRX = SRX647918 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T15:09:09 prefetch.2.10.7: 1) Downloading 'SRR1508954'... 2020-06-29T15:09:09 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:11:51 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:11:51 prefetch.2.10.7: 1) 'SRR1508954' was downloaded successfully 2020-06-29T15:11:51 prefetch.2.10.7: 'SRR1508954' has 0 unresolved dependencies Read 22022780 spots for SRR1508954/SRR1508954.sra Written 22022780 spots for SRR1508954/SRR1508954.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:14 22022780 reads; of these: 22022780 (100.00%) were unpaired; of these: 818123 (3.71%) aligned 0 times 16163853 (73.40%) aligned exactly 1 time 5040804 (22.89%) aligned >1 times 96.29% overall alignment rate Time searching: 00:07:14 Overall time: 00:07:14 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 2053558 / 21204657 = 0.0968 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:31:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX647918/SRX647918.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX647918/SRX647918.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX647918/SRX647918.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX647918/SRX647918.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:31:07: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:31:07: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:31:12: 1000000 INFO @ Tue, 30 Jun 2020 00:31:17: 2000000 INFO @ Tue, 30 Jun 2020 00:31:21: 3000000 INFO @ Tue, 30 Jun 2020 00:31:26: 4000000 INFO @ Tue, 30 Jun 2020 00:31:31: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:31:36: 6000000 INFO @ Tue, 30 Jun 2020 00:31:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX647918/SRX647918.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX647918/SRX647918.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX647918/SRX647918.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX647918/SRX647918.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:31:37: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:31:37: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:31:41: 7000000 INFO @ Tue, 30 Jun 2020 00:31:42: 1000000 INFO @ Tue, 30 Jun 2020 00:31:46: 8000000 INFO @ Tue, 30 Jun 2020 00:31:47: 2000000 INFO @ Tue, 30 Jun 2020 00:31:51: 9000000 INFO @ Tue, 30 Jun 2020 00:31:52: 3000000 INFO @ Tue, 30 Jun 2020 00:31:55: 10000000 INFO @ Tue, 30 Jun 2020 00:31:57: 4000000 INFO @ Tue, 30 Jun 2020 00:32:00: 11000000 INFO @ Tue, 30 Jun 2020 00:32:02: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:32:05: 12000000 INFO @ Tue, 30 Jun 2020 00:32:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX647918/SRX647918.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX647918/SRX647918.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX647918/SRX647918.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX647918/SRX647918.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:32:07: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:32:07: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:32:08: 6000000 INFO @ Tue, 30 Jun 2020 00:32:10: 13000000 INFO @ Tue, 30 Jun 2020 00:32:13: 1000000 INFO @ Tue, 30 Jun 2020 00:32:13: 7000000 INFO @ Tue, 30 Jun 2020 00:32:15: 14000000 INFO @ Tue, 30 Jun 2020 00:32:18: 8000000 INFO @ Tue, 30 Jun 2020 00:32:19: 2000000 INFO @ Tue, 30 Jun 2020 00:32:20: 15000000 INFO @ Tue, 30 Jun 2020 00:32:23: 9000000 INFO @ Tue, 30 Jun 2020 00:32:24: 3000000 INFO @ Tue, 30 Jun 2020 00:32:25: 16000000 INFO @ Tue, 30 Jun 2020 00:32:28: 10000000 INFO @ Tue, 30 Jun 2020 00:32:30: 4000000 INFO @ Tue, 30 Jun 2020 00:32:30: 17000000 INFO @ Tue, 30 Jun 2020 00:32:33: 11000000 INFO @ Tue, 30 Jun 2020 00:32:36: 18000000 INFO @ Tue, 30 Jun 2020 00:32:36: 5000000 INFO @ Tue, 30 Jun 2020 00:32:38: 12000000 INFO @ Tue, 30 Jun 2020 00:32:41: 19000000 INFO @ Tue, 30 Jun 2020 00:32:41: 6000000 INFO @ Tue, 30 Jun 2020 00:32:42: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:32:42: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:32:42: #1 total tags in treatment: 19151099 INFO @ Tue, 30 Jun 2020 00:32:42: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:32:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:32:42: #1 tags after filtering in treatment: 19151099 INFO @ Tue, 30 Jun 2020 00:32:42: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:32:42: #1 finished! INFO @ Tue, 30 Jun 2020 00:32:42: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:32:42: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:32:43: #2 number of paired peaks: 68 WARNING @ Tue, 30 Jun 2020 00:32:43: Too few paired peaks (68) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:32:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX647918/SRX647918.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX647918/SRX647918.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX647918/SRX647918.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX647918/SRX647918.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:32:43: 13000000 INFO @ Tue, 30 Jun 2020 00:32:47: 7000000 INFO @ Tue, 30 Jun 2020 00:32:48: 14000000 INFO @ Tue, 30 Jun 2020 00:32:53: 8000000 INFO @ Tue, 30 Jun 2020 00:32:53: 15000000 INFO @ Tue, 30 Jun 2020 00:32:58: 9000000 INFO @ Tue, 30 Jun 2020 00:32:59: 16000000 INFO @ Tue, 30 Jun 2020 00:33:04: 17000000 INFO @ Tue, 30 Jun 2020 00:33:04: 10000000 INFO @ Tue, 30 Jun 2020 00:33:09: 18000000 INFO @ Tue, 30 Jun 2020 00:33:09: 11000000 INFO @ Tue, 30 Jun 2020 00:33:14: 19000000 INFO @ Tue, 30 Jun 2020 00:33:15: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:33:15: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:33:15: #1 total tags in treatment: 19151099 INFO @ Tue, 30 Jun 2020 00:33:15: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:33:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:33:15: #1 tags after filtering in treatment: 19151099 INFO @ Tue, 30 Jun 2020 00:33:15: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:33:15: #1 finished! INFO @ Tue, 30 Jun 2020 00:33:15: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:33:15: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:33:15: 12000000 INFO @ Tue, 30 Jun 2020 00:33:16: #2 number of paired peaks: 68 WARNING @ Tue, 30 Jun 2020 00:33:16: Too few paired peaks (68) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:33:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX647918/SRX647918.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX647918/SRX647918.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX647918/SRX647918.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX647918/SRX647918.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:33:21: 13000000 INFO @ Tue, 30 Jun 2020 00:33:26: 14000000 INFO @ Tue, 30 Jun 2020 00:33:32: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:33:37: 16000000 INFO @ Tue, 30 Jun 2020 00:33:43: 17000000 INFO @ Tue, 30 Jun 2020 00:33:48: 18000000 INFO @ Tue, 30 Jun 2020 00:33:54: 19000000 INFO @ Tue, 30 Jun 2020 00:33:55: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:33:55: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:33:55: #1 total tags in treatment: 19151099 INFO @ Tue, 30 Jun 2020 00:33:55: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:33:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:33:55: #1 tags after filtering in treatment: 19151099 INFO @ Tue, 30 Jun 2020 00:33:55: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:33:55: #1 finished! INFO @ Tue, 30 Jun 2020 00:33:55: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:33:55: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:33:56: #2 number of paired peaks: 68 WARNING @ Tue, 30 Jun 2020 00:33:56: Too few paired peaks (68) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:33:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX647918/SRX647918.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX647918/SRX647918.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX647918/SRX647918.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX647918/SRX647918.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。