Job ID = 4178633 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 37,492,837 reads read : 37,492,837 reads written : 37,492,837 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:17 37492837 reads; of these: 37492837 (100.00%) were unpaired; of these: 8503426 (22.68%) aligned 0 times 18832641 (50.23%) aligned exactly 1 time 10156770 (27.09%) aligned >1 times 77.32% overall alignment rate Time searching: 00:12:17 Overall time: 00:12:17 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 9305383 / 28989411 = 0.3210 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Thu, 05 Dec 2019 13:58:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6468507/SRX6468507.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6468507/SRX6468507.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6468507/SRX6468507.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6468507/SRX6468507.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Dec 2019 13:58:06: #1 read tag files... INFO @ Thu, 05 Dec 2019 13:58:06: #1 read treatment tags... INFO @ Thu, 05 Dec 2019 13:58:12: 1000000 INFO @ Thu, 05 Dec 2019 13:58:19: 2000000 INFO @ Thu, 05 Dec 2019 13:58:25: 3000000 INFO @ Thu, 05 Dec 2019 13:58:31: 4000000 INFO @ Thu, 05 Dec 2019 13:58:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6468507/SRX6468507.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6468507/SRX6468507.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6468507/SRX6468507.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6468507/SRX6468507.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Dec 2019 13:58:36: #1 read tag files... INFO @ Thu, 05 Dec 2019 13:58:36: #1 read treatment tags... INFO @ Thu, 05 Dec 2019 13:58:37: 5000000 INFO @ Thu, 05 Dec 2019 13:58:42: 1000000 INFO @ Thu, 05 Dec 2019 13:58:44: 6000000 INFO @ Thu, 05 Dec 2019 13:58:48: 2000000 INFO @ Thu, 05 Dec 2019 13:58:50: 7000000 INFO @ Thu, 05 Dec 2019 13:58:53: 3000000 INFO @ Thu, 05 Dec 2019 13:58:56: 8000000 INFO @ Thu, 05 Dec 2019 13:58:59: 4000000 INFO @ Thu, 05 Dec 2019 13:59:02: 9000000 BedGraph に変換中... INFO @ Thu, 05 Dec 2019 13:59:05: 5000000 INFO @ Thu, 05 Dec 2019 13:59:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6468507/SRX6468507.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6468507/SRX6468507.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6468507/SRX6468507.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6468507/SRX6468507.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Dec 2019 13:59:06: #1 read tag files... INFO @ Thu, 05 Dec 2019 13:59:06: #1 read treatment tags... INFO @ Thu, 05 Dec 2019 13:59:08: 10000000 INFO @ Thu, 05 Dec 2019 13:59:11: 6000000 INFO @ Thu, 05 Dec 2019 13:59:12: 1000000 INFO @ Thu, 05 Dec 2019 13:59:15: 11000000 INFO @ Thu, 05 Dec 2019 13:59:17: 7000000 INFO @ Thu, 05 Dec 2019 13:59:19: 2000000 INFO @ Thu, 05 Dec 2019 13:59:21: 12000000 INFO @ Thu, 05 Dec 2019 13:59:24: 8000000 INFO @ Thu, 05 Dec 2019 13:59:25: 3000000 INFO @ Thu, 05 Dec 2019 13:59:27: 13000000 INFO @ Thu, 05 Dec 2019 13:59:30: 9000000 INFO @ Thu, 05 Dec 2019 13:59:32: 4000000 INFO @ Thu, 05 Dec 2019 13:59:33: 14000000 INFO @ Thu, 05 Dec 2019 13:59:37: 10000000 INFO @ Thu, 05 Dec 2019 13:59:38: 5000000 INFO @ Thu, 05 Dec 2019 13:59:39: 15000000 INFO @ Thu, 05 Dec 2019 13:59:43: 11000000 INFO @ Thu, 05 Dec 2019 13:59:44: 6000000 INFO @ Thu, 05 Dec 2019 13:59:45: 16000000 INFO @ Thu, 05 Dec 2019 13:59:49: 12000000 INFO @ Thu, 05 Dec 2019 13:59:51: 7000000 INFO @ Thu, 05 Dec 2019 13:59:51: 17000000 INFO @ Thu, 05 Dec 2019 13:59:56: 13000000 INFO @ Thu, 05 Dec 2019 13:59:57: 8000000 INFO @ Thu, 05 Dec 2019 13:59:58: 18000000 INFO @ Thu, 05 Dec 2019 14:00:02: 14000000 INFO @ Thu, 05 Dec 2019 14:00:03: 9000000 INFO @ Thu, 05 Dec 2019 14:00:04: 19000000 INFO @ Thu, 05 Dec 2019 14:00:08: #1 tag size is determined as 50 bps INFO @ Thu, 05 Dec 2019 14:00:08: #1 tag size = 50 INFO @ Thu, 05 Dec 2019 14:00:08: #1 total tags in treatment: 19684028 INFO @ Thu, 05 Dec 2019 14:00:08: #1 user defined the maximum tags... INFO @ Thu, 05 Dec 2019 14:00:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Dec 2019 14:00:08: #1 tags after filtering in treatment: 19684028 INFO @ Thu, 05 Dec 2019 14:00:08: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Dec 2019 14:00:08: #1 finished! INFO @ Thu, 05 Dec 2019 14:00:08: #2 Build Peak Model... INFO @ Thu, 05 Dec 2019 14:00:08: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Dec 2019 14:00:08: 15000000 INFO @ Thu, 05 Dec 2019 14:00:09: #2 number of paired peaks: 4 WARNING @ Thu, 05 Dec 2019 14:00:09: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Dec 2019 14:00:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6468507/SRX6468507.05_peaks.narrowPeak: No such file or directory INFO @ Thu, 05 Dec 2019 14:00:10: 10000000 pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468507/SRX6468507.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468507/SRX6468507.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468507/SRX6468507.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 05 Dec 2019 14:00:15: 16000000 INFO @ Thu, 05 Dec 2019 14:00:16: 11000000 INFO @ Thu, 05 Dec 2019 14:00:21: 17000000 INFO @ Thu, 05 Dec 2019 14:00:22: 12000000 INFO @ Thu, 05 Dec 2019 14:00:27: 18000000 INFO @ Thu, 05 Dec 2019 14:00:28: 13000000 INFO @ Thu, 05 Dec 2019 14:00:33: 19000000 INFO @ Thu, 05 Dec 2019 14:00:35: 14000000 INFO @ Thu, 05 Dec 2019 14:00:37: #1 tag size is determined as 50 bps INFO @ Thu, 05 Dec 2019 14:00:37: #1 tag size = 50 INFO @ Thu, 05 Dec 2019 14:00:37: #1 total tags in treatment: 19684028 INFO @ Thu, 05 Dec 2019 14:00:37: #1 user defined the maximum tags... INFO @ Thu, 05 Dec 2019 14:00:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Dec 2019 14:00:38: #1 tags after filtering in treatment: 19684028 INFO @ Thu, 05 Dec 2019 14:00:38: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Dec 2019 14:00:38: #1 finished! INFO @ Thu, 05 Dec 2019 14:00:38: #2 Build Peak Model... INFO @ Thu, 05 Dec 2019 14:00:38: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Dec 2019 14:00:39: #2 number of paired peaks: 4 WARNING @ Thu, 05 Dec 2019 14:00:39: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Dec 2019 14:00:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6468507/SRX6468507.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468507/SRX6468507.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468507/SRX6468507.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468507/SRX6468507.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 05 Dec 2019 14:00:40: 15000000 INFO @ Thu, 05 Dec 2019 14:00:46: 16000000 INFO @ Thu, 05 Dec 2019 14:00:52: 17000000 INFO @ Thu, 05 Dec 2019 14:00:57: 18000000 INFO @ Thu, 05 Dec 2019 14:01:03: 19000000 INFO @ Thu, 05 Dec 2019 14:01:07: #1 tag size is determined as 50 bps INFO @ Thu, 05 Dec 2019 14:01:07: #1 tag size = 50 INFO @ Thu, 05 Dec 2019 14:01:07: #1 total tags in treatment: 19684028 INFO @ Thu, 05 Dec 2019 14:01:07: #1 user defined the maximum tags... INFO @ Thu, 05 Dec 2019 14:01:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Dec 2019 14:01:07: #1 tags after filtering in treatment: 19684028 INFO @ Thu, 05 Dec 2019 14:01:07: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Dec 2019 14:01:07: #1 finished! INFO @ Thu, 05 Dec 2019 14:01:07: #2 Build Peak Model... INFO @ Thu, 05 Dec 2019 14:01:07: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Dec 2019 14:01:08: #2 number of paired peaks: 4 WARNING @ Thu, 05 Dec 2019 14:01:08: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Dec 2019 14:01:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6468507/SRX6468507.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468507/SRX6468507.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468507/SRX6468507.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468507/SRX6468507.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。