Job ID = 4178632 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 29,759,533 reads read : 29,759,533 reads written : 29,759,533 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:59 29759533 reads; of these: 29759533 (100.00%) were unpaired; of these: 7109943 (23.89%) aligned 0 times 15280598 (51.35%) aligned exactly 1 time 7368992 (24.76%) aligned >1 times 76.11% overall alignment rate Time searching: 00:11:59 Overall time: 00:11:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 8387796 / 22649590 = 0.3703 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Thu, 05 Dec 2019 13:53:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6468506/SRX6468506.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6468506/SRX6468506.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6468506/SRX6468506.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6468506/SRX6468506.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Dec 2019 13:53:29: #1 read tag files... INFO @ Thu, 05 Dec 2019 13:53:29: #1 read treatment tags... INFO @ Thu, 05 Dec 2019 13:53:38: 1000000 INFO @ Thu, 05 Dec 2019 13:53:48: 2000000 INFO @ Thu, 05 Dec 2019 13:53:56: 3000000 INFO @ Thu, 05 Dec 2019 13:53:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6468506/SRX6468506.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6468506/SRX6468506.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6468506/SRX6468506.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6468506/SRX6468506.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Dec 2019 13:53:58: #1 read tag files... INFO @ Thu, 05 Dec 2019 13:53:58: #1 read treatment tags... INFO @ Thu, 05 Dec 2019 13:54:04: 4000000 INFO @ Thu, 05 Dec 2019 13:54:06: 1000000 INFO @ Thu, 05 Dec 2019 13:54:13: 5000000 INFO @ Thu, 05 Dec 2019 13:54:15: 2000000 INFO @ Thu, 05 Dec 2019 13:54:23: 6000000 BedGraph に変換中... INFO @ Thu, 05 Dec 2019 13:54:25: 3000000 INFO @ Thu, 05 Dec 2019 13:54:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6468506/SRX6468506.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6468506/SRX6468506.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6468506/SRX6468506.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6468506/SRX6468506.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Dec 2019 13:54:28: #1 read tag files... INFO @ Thu, 05 Dec 2019 13:54:28: #1 read treatment tags... INFO @ Thu, 05 Dec 2019 13:54:32: 7000000 INFO @ Thu, 05 Dec 2019 13:54:35: 4000000 INFO @ Thu, 05 Dec 2019 13:54:37: 1000000 INFO @ Thu, 05 Dec 2019 13:54:41: 8000000 INFO @ Thu, 05 Dec 2019 13:54:44: 5000000 INFO @ Thu, 05 Dec 2019 13:54:45: 2000000 INFO @ Thu, 05 Dec 2019 13:54:51: 9000000 INFO @ Thu, 05 Dec 2019 13:54:54: 6000000 INFO @ Thu, 05 Dec 2019 13:54:56: 3000000 INFO @ Thu, 05 Dec 2019 13:55:01: 10000000 INFO @ Thu, 05 Dec 2019 13:55:03: 7000000 INFO @ Thu, 05 Dec 2019 13:55:04: 4000000 INFO @ Thu, 05 Dec 2019 13:55:10: 11000000 INFO @ Thu, 05 Dec 2019 13:55:13: 5000000 INFO @ Thu, 05 Dec 2019 13:55:13: 8000000 INFO @ Thu, 05 Dec 2019 13:55:19: 12000000 INFO @ Thu, 05 Dec 2019 13:55:22: 6000000 INFO @ Thu, 05 Dec 2019 13:55:22: 9000000 INFO @ Thu, 05 Dec 2019 13:55:28: 13000000 INFO @ Thu, 05 Dec 2019 13:55:30: 7000000 INFO @ Thu, 05 Dec 2019 13:55:31: 10000000 INFO @ Thu, 05 Dec 2019 13:55:39: 14000000 INFO @ Thu, 05 Dec 2019 13:55:40: 8000000 INFO @ Thu, 05 Dec 2019 13:55:41: 11000000 INFO @ Thu, 05 Dec 2019 13:55:42: #1 tag size is determined as 50 bps INFO @ Thu, 05 Dec 2019 13:55:42: #1 tag size = 50 INFO @ Thu, 05 Dec 2019 13:55:42: #1 total tags in treatment: 14261794 INFO @ Thu, 05 Dec 2019 13:55:42: #1 user defined the maximum tags... INFO @ Thu, 05 Dec 2019 13:55:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Dec 2019 13:55:42: #1 tags after filtering in treatment: 14261794 INFO @ Thu, 05 Dec 2019 13:55:42: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Dec 2019 13:55:42: #1 finished! INFO @ Thu, 05 Dec 2019 13:55:42: #2 Build Peak Model... INFO @ Thu, 05 Dec 2019 13:55:42: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Dec 2019 13:55:44: #2 number of paired peaks: 22 WARNING @ Thu, 05 Dec 2019 13:55:44: Too few paired peaks (22) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Dec 2019 13:55:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6468506/SRX6468506.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468506/SRX6468506.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468506/SRX6468506.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468506/SRX6468506.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 05 Dec 2019 13:55:52: 9000000 INFO @ Thu, 05 Dec 2019 13:55:53: 12000000 INFO @ Thu, 05 Dec 2019 13:56:03: 10000000 INFO @ Thu, 05 Dec 2019 13:56:04: 13000000 INFO @ Thu, 05 Dec 2019 13:56:14: 11000000 INFO @ Thu, 05 Dec 2019 13:56:15: 14000000 INFO @ Thu, 05 Dec 2019 13:56:18: #1 tag size is determined as 50 bps INFO @ Thu, 05 Dec 2019 13:56:18: #1 tag size = 50 INFO @ Thu, 05 Dec 2019 13:56:18: #1 total tags in treatment: 14261794 INFO @ Thu, 05 Dec 2019 13:56:18: #1 user defined the maximum tags... INFO @ Thu, 05 Dec 2019 13:56:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Dec 2019 13:56:18: #1 tags after filtering in treatment: 14261794 INFO @ Thu, 05 Dec 2019 13:56:18: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Dec 2019 13:56:18: #1 finished! INFO @ Thu, 05 Dec 2019 13:56:18: #2 Build Peak Model... INFO @ Thu, 05 Dec 2019 13:56:18: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Dec 2019 13:56:20: #2 number of paired peaks: 22 WARNING @ Thu, 05 Dec 2019 13:56:20: Too few paired peaks (22) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Dec 2019 13:56:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6468506/SRX6468506.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468506/SRX6468506.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468506/SRX6468506.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468506/SRX6468506.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 05 Dec 2019 13:56:25: 12000000 INFO @ Thu, 05 Dec 2019 13:56:35: 13000000 INFO @ Thu, 05 Dec 2019 13:56:45: 14000000 INFO @ Thu, 05 Dec 2019 13:56:48: #1 tag size is determined as 50 bps INFO @ Thu, 05 Dec 2019 13:56:48: #1 tag size = 50 INFO @ Thu, 05 Dec 2019 13:56:48: #1 total tags in treatment: 14261794 INFO @ Thu, 05 Dec 2019 13:56:48: #1 user defined the maximum tags... INFO @ Thu, 05 Dec 2019 13:56:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Dec 2019 13:56:48: #1 tags after filtering in treatment: 14261794 INFO @ Thu, 05 Dec 2019 13:56:48: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Dec 2019 13:56:48: #1 finished! INFO @ Thu, 05 Dec 2019 13:56:48: #2 Build Peak Model... INFO @ Thu, 05 Dec 2019 13:56:48: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Dec 2019 13:56:49: #2 number of paired peaks: 22 WARNING @ Thu, 05 Dec 2019 13:56:49: Too few paired peaks (22) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Dec 2019 13:56:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6468506/SRX6468506.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468506/SRX6468506.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468506/SRX6468506.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468506/SRX6468506.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。