Job ID = 6528450 SRX = SRX6468505 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T15:11:20 prefetch.2.10.7: 1) Downloading 'SRR9710591'... 2020-06-29T15:11:20 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:14:47 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:14:47 prefetch.2.10.7: 1) 'SRR9710591' was downloaded successfully Read 30440827 spots for SRR9710591/SRR9710591.sra Written 30440827 spots for SRR9710591/SRR9710591.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:01 30440827 reads; of these: 30440827 (100.00%) were unpaired; of these: 2712319 (8.91%) aligned 0 times 19602255 (64.39%) aligned exactly 1 time 8126253 (26.70%) aligned >1 times 91.09% overall alignment rate Time searching: 00:10:01 Overall time: 00:10:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 10495743 / 27728508 = 0.3785 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:39:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6468505/SRX6468505.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6468505/SRX6468505.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6468505/SRX6468505.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6468505/SRX6468505.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:39:25: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:39:25: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:39:31: 1000000 INFO @ Tue, 30 Jun 2020 00:39:37: 2000000 INFO @ Tue, 30 Jun 2020 00:39:42: 3000000 INFO @ Tue, 30 Jun 2020 00:39:48: 4000000 INFO @ Tue, 30 Jun 2020 00:39:54: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:39:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6468505/SRX6468505.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6468505/SRX6468505.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6468505/SRX6468505.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6468505/SRX6468505.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:39:55: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:39:55: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:40:00: 6000000 INFO @ Tue, 30 Jun 2020 00:40:03: 1000000 INFO @ Tue, 30 Jun 2020 00:40:06: 7000000 INFO @ Tue, 30 Jun 2020 00:40:10: 2000000 INFO @ Tue, 30 Jun 2020 00:40:13: 8000000 INFO @ Tue, 30 Jun 2020 00:40:17: 3000000 INFO @ Tue, 30 Jun 2020 00:40:19: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:40:25: 4000000 INFO @ Tue, 30 Jun 2020 00:40:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6468505/SRX6468505.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6468505/SRX6468505.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6468505/SRX6468505.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6468505/SRX6468505.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:40:25: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:40:25: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:40:26: 10000000 INFO @ Tue, 30 Jun 2020 00:40:32: 1000000 INFO @ Tue, 30 Jun 2020 00:40:32: 5000000 INFO @ Tue, 30 Jun 2020 00:40:32: 11000000 INFO @ Tue, 30 Jun 2020 00:40:39: 2000000 INFO @ Tue, 30 Jun 2020 00:40:39: 12000000 INFO @ Tue, 30 Jun 2020 00:40:39: 6000000 INFO @ Tue, 30 Jun 2020 00:40:45: 3000000 INFO @ Tue, 30 Jun 2020 00:40:45: 13000000 INFO @ Tue, 30 Jun 2020 00:40:47: 7000000 INFO @ Tue, 30 Jun 2020 00:40:52: 14000000 INFO @ Tue, 30 Jun 2020 00:40:52: 4000000 INFO @ Tue, 30 Jun 2020 00:40:54: 8000000 INFO @ Tue, 30 Jun 2020 00:40:58: 5000000 INFO @ Tue, 30 Jun 2020 00:40:58: 15000000 INFO @ Tue, 30 Jun 2020 00:41:01: 9000000 INFO @ Tue, 30 Jun 2020 00:41:05: 16000000 INFO @ Tue, 30 Jun 2020 00:41:05: 6000000 INFO @ Tue, 30 Jun 2020 00:41:08: 10000000 INFO @ Tue, 30 Jun 2020 00:41:11: 17000000 INFO @ Tue, 30 Jun 2020 00:41:11: 7000000 INFO @ Tue, 30 Jun 2020 00:41:13: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:41:13: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:41:13: #1 total tags in treatment: 17232765 INFO @ Tue, 30 Jun 2020 00:41:13: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:41:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:41:13: #1 tags after filtering in treatment: 17232765 INFO @ Tue, 30 Jun 2020 00:41:13: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:41:13: #1 finished! INFO @ Tue, 30 Jun 2020 00:41:13: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:41:13: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:41:14: #2 number of paired peaks: 11 WARNING @ Tue, 30 Jun 2020 00:41:14: Too few paired peaks (11) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:41:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6468505/SRX6468505.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468505/SRX6468505.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468505/SRX6468505.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468505/SRX6468505.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:41:15: 11000000 INFO @ Tue, 30 Jun 2020 00:41:18: 8000000 INFO @ Tue, 30 Jun 2020 00:41:22: 12000000 INFO @ Tue, 30 Jun 2020 00:41:24: 9000000 INFO @ Tue, 30 Jun 2020 00:41:29: 13000000 INFO @ Tue, 30 Jun 2020 00:41:31: 10000000 INFO @ Tue, 30 Jun 2020 00:41:37: 14000000 INFO @ Tue, 30 Jun 2020 00:41:37: 11000000 INFO @ Tue, 30 Jun 2020 00:41:43: 12000000 INFO @ Tue, 30 Jun 2020 00:41:44: 15000000 INFO @ Tue, 30 Jun 2020 00:41:50: 13000000 INFO @ Tue, 30 Jun 2020 00:41:51: 16000000 INFO @ Tue, 30 Jun 2020 00:41:56: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:41:58: 17000000 INFO @ Tue, 30 Jun 2020 00:41:59: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:41:59: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:41:59: #1 total tags in treatment: 17232765 INFO @ Tue, 30 Jun 2020 00:41:59: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:41:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:42:00: #1 tags after filtering in treatment: 17232765 INFO @ Tue, 30 Jun 2020 00:42:00: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:42:00: #1 finished! INFO @ Tue, 30 Jun 2020 00:42:00: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:42:00: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:42:01: #2 number of paired peaks: 11 WARNING @ Tue, 30 Jun 2020 00:42:01: Too few paired peaks (11) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:42:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6468505/SRX6468505.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468505/SRX6468505.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468505/SRX6468505.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468505/SRX6468505.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:42:02: 15000000 INFO @ Tue, 30 Jun 2020 00:42:08: 16000000 INFO @ Tue, 30 Jun 2020 00:42:14: 17000000 INFO @ Tue, 30 Jun 2020 00:42:16: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:42:16: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:42:16: #1 total tags in treatment: 17232765 INFO @ Tue, 30 Jun 2020 00:42:16: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:42:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:42:16: #1 tags after filtering in treatment: 17232765 INFO @ Tue, 30 Jun 2020 00:42:16: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:42:16: #1 finished! INFO @ Tue, 30 Jun 2020 00:42:16: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:42:16: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:42:17: #2 number of paired peaks: 11 WARNING @ Tue, 30 Jun 2020 00:42:17: Too few paired peaks (11) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:42:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6468505/SRX6468505.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468505/SRX6468505.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468505/SRX6468505.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468505/SRX6468505.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。