Job ID = 6528449 SRX = SRX6468504 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T15:47:07 prefetch.2.10.7: 1) Downloading 'SRR9710590'... 2020-06-29T15:47:07 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:49:46 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:49:46 prefetch.2.10.7: 1) 'SRR9710590' was downloaded successfully Read 26072395 spots for SRR9710590/SRR9710590.sra Written 26072395 spots for SRR9710590/SRR9710590.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:31 26072395 reads; of these: 26072395 (100.00%) were unpaired; of these: 2337700 (8.97%) aligned 0 times 16583722 (63.61%) aligned exactly 1 time 7150973 (27.43%) aligned >1 times 91.03% overall alignment rate Time searching: 00:09:31 Overall time: 00:09:31 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 7630849 / 23734695 = 0.3215 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:15:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6468504/SRX6468504.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6468504/SRX6468504.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6468504/SRX6468504.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6468504/SRX6468504.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:15:42: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:15:42: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:15:49: 1000000 INFO @ Tue, 30 Jun 2020 01:15:57: 2000000 INFO @ Tue, 30 Jun 2020 01:16:04: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:16:11: 4000000 INFO @ Tue, 30 Jun 2020 01:16:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6468504/SRX6468504.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6468504/SRX6468504.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6468504/SRX6468504.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6468504/SRX6468504.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:16:12: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:16:12: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:16:18: 5000000 INFO @ Tue, 30 Jun 2020 01:16:21: 1000000 INFO @ Tue, 30 Jun 2020 01:16:26: 6000000 INFO @ Tue, 30 Jun 2020 01:16:30: 2000000 INFO @ Tue, 30 Jun 2020 01:16:33: 7000000 INFO @ Tue, 30 Jun 2020 01:16:38: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:16:41: 8000000 INFO @ Tue, 30 Jun 2020 01:16:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6468504/SRX6468504.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6468504/SRX6468504.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6468504/SRX6468504.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6468504/SRX6468504.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:16:42: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:16:42: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:16:46: 4000000 INFO @ Tue, 30 Jun 2020 01:16:49: 9000000 INFO @ Tue, 30 Jun 2020 01:16:50: 1000000 INFO @ Tue, 30 Jun 2020 01:16:54: 5000000 INFO @ Tue, 30 Jun 2020 01:16:58: 2000000 INFO @ Tue, 30 Jun 2020 01:16:58: 10000000 INFO @ Tue, 30 Jun 2020 01:17:02: 6000000 INFO @ Tue, 30 Jun 2020 01:17:05: 3000000 INFO @ Tue, 30 Jun 2020 01:17:06: 11000000 INFO @ Tue, 30 Jun 2020 01:17:10: 7000000 INFO @ Tue, 30 Jun 2020 01:17:13: 4000000 INFO @ Tue, 30 Jun 2020 01:17:14: 12000000 INFO @ Tue, 30 Jun 2020 01:17:19: 8000000 INFO @ Tue, 30 Jun 2020 01:17:20: 5000000 INFO @ Tue, 30 Jun 2020 01:17:23: 13000000 INFO @ Tue, 30 Jun 2020 01:17:27: 6000000 INFO @ Tue, 30 Jun 2020 01:17:27: 9000000 INFO @ Tue, 30 Jun 2020 01:17:32: 14000000 INFO @ Tue, 30 Jun 2020 01:17:34: 7000000 INFO @ Tue, 30 Jun 2020 01:17:35: 10000000 INFO @ Tue, 30 Jun 2020 01:17:40: 15000000 INFO @ Tue, 30 Jun 2020 01:17:41: 8000000 INFO @ Tue, 30 Jun 2020 01:17:44: 11000000 INFO @ Tue, 30 Jun 2020 01:17:48: 9000000 INFO @ Tue, 30 Jun 2020 01:17:48: 16000000 INFO @ Tue, 30 Jun 2020 01:17:49: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 01:17:49: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 01:17:49: #1 total tags in treatment: 16103846 INFO @ Tue, 30 Jun 2020 01:17:49: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:17:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:17:49: #1 tags after filtering in treatment: 16103846 INFO @ Tue, 30 Jun 2020 01:17:49: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:17:49: #1 finished! INFO @ Tue, 30 Jun 2020 01:17:49: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:17:49: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:17:50: #2 number of paired peaks: 9 WARNING @ Tue, 30 Jun 2020 01:17:50: Too few paired peaks (9) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:17:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6468504/SRX6468504.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468504/SRX6468504.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468504/SRX6468504.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468504/SRX6468504.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 01:17:52: 12000000 INFO @ Tue, 30 Jun 2020 01:17:55: 10000000 INFO @ Tue, 30 Jun 2020 01:17:59: 13000000 INFO @ Tue, 30 Jun 2020 01:18:04: 11000000 INFO @ Tue, 30 Jun 2020 01:18:07: 14000000 INFO @ Tue, 30 Jun 2020 01:18:11: 12000000 INFO @ Tue, 30 Jun 2020 01:18:15: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 01:18:18: 13000000 INFO @ Tue, 30 Jun 2020 01:18:22: 16000000 INFO @ Tue, 30 Jun 2020 01:18:23: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 01:18:23: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 01:18:23: #1 total tags in treatment: 16103846 INFO @ Tue, 30 Jun 2020 01:18:23: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:18:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:18:23: #1 tags after filtering in treatment: 16103846 INFO @ Tue, 30 Jun 2020 01:18:23: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:18:23: #1 finished! INFO @ Tue, 30 Jun 2020 01:18:23: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:18:23: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:18:25: #2 number of paired peaks: 9 WARNING @ Tue, 30 Jun 2020 01:18:25: Too few paired peaks (9) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:18:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6468504/SRX6468504.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468504/SRX6468504.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468504/SRX6468504.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468504/SRX6468504.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 01:18:25: 14000000 INFO @ Tue, 30 Jun 2020 01:18:32: 15000000 INFO @ Tue, 30 Jun 2020 01:18:39: 16000000 INFO @ Tue, 30 Jun 2020 01:18:39: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 01:18:39: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 01:18:39: #1 total tags in treatment: 16103846 INFO @ Tue, 30 Jun 2020 01:18:39: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:18:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:18:40: #1 tags after filtering in treatment: 16103846 INFO @ Tue, 30 Jun 2020 01:18:40: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:18:40: #1 finished! INFO @ Tue, 30 Jun 2020 01:18:40: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:18:40: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:18:41: #2 number of paired peaks: 9 WARNING @ Tue, 30 Jun 2020 01:18:41: Too few paired peaks (9) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:18:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6468504/SRX6468504.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468504/SRX6468504.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468504/SRX6468504.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468504/SRX6468504.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。