Job ID = 4178624 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 26,759,923 reads read : 26,759,923 reads written : 26,759,923 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:36 26759923 reads; of these: 26759923 (100.00%) were unpaired; of these: 3141680 (11.74%) aligned 0 times 15585835 (58.24%) aligned exactly 1 time 8032408 (30.02%) aligned >1 times 88.26% overall alignment rate Time searching: 00:09:37 Overall time: 00:09:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 6524243 / 23618243 = 0.2762 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Thu, 05 Dec 2019 13:45:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6468498/SRX6468498.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6468498/SRX6468498.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6468498/SRX6468498.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6468498/SRX6468498.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Dec 2019 13:45:58: #1 read tag files... INFO @ Thu, 05 Dec 2019 13:45:58: #1 read treatment tags... INFO @ Thu, 05 Dec 2019 13:46:04: 1000000 INFO @ Thu, 05 Dec 2019 13:46:10: 2000000 INFO @ Thu, 05 Dec 2019 13:46:16: 3000000 INFO @ Thu, 05 Dec 2019 13:46:22: 4000000 INFO @ Thu, 05 Dec 2019 13:46:28: 5000000 INFO @ Thu, 05 Dec 2019 13:46:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6468498/SRX6468498.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6468498/SRX6468498.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6468498/SRX6468498.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6468498/SRX6468498.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Dec 2019 13:46:28: #1 read tag files... INFO @ Thu, 05 Dec 2019 13:46:28: #1 read treatment tags... INFO @ Thu, 05 Dec 2019 13:46:34: 1000000 INFO @ Thu, 05 Dec 2019 13:46:34: 6000000 INFO @ Thu, 05 Dec 2019 13:46:39: 2000000 INFO @ Thu, 05 Dec 2019 13:46:40: 7000000 INFO @ Thu, 05 Dec 2019 13:46:45: 3000000 INFO @ Thu, 05 Dec 2019 13:46:46: 8000000 INFO @ Thu, 05 Dec 2019 13:46:50: 4000000 INFO @ Thu, 05 Dec 2019 13:46:53: 9000000 INFO @ Thu, 05 Dec 2019 13:46:56: 5000000 BedGraph に変換中... INFO @ Thu, 05 Dec 2019 13:46:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6468498/SRX6468498.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6468498/SRX6468498.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6468498/SRX6468498.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6468498/SRX6468498.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Dec 2019 13:46:58: #1 read tag files... INFO @ Thu, 05 Dec 2019 13:46:58: #1 read treatment tags... INFO @ Thu, 05 Dec 2019 13:46:59: 10000000 INFO @ Thu, 05 Dec 2019 13:47:02: 6000000 INFO @ Thu, 05 Dec 2019 13:47:04: 1000000 INFO @ Thu, 05 Dec 2019 13:47:05: 11000000 INFO @ Thu, 05 Dec 2019 13:47:08: 7000000 INFO @ Thu, 05 Dec 2019 13:47:10: 2000000 INFO @ Thu, 05 Dec 2019 13:47:11: 12000000 INFO @ Thu, 05 Dec 2019 13:47:14: 8000000 INFO @ Thu, 05 Dec 2019 13:47:16: 3000000 INFO @ Thu, 05 Dec 2019 13:47:18: 13000000 INFO @ Thu, 05 Dec 2019 13:47:20: 9000000 INFO @ Thu, 05 Dec 2019 13:47:22: 4000000 INFO @ Thu, 05 Dec 2019 13:47:24: 14000000 INFO @ Thu, 05 Dec 2019 13:47:26: 10000000 INFO @ Thu, 05 Dec 2019 13:47:28: 5000000 INFO @ Thu, 05 Dec 2019 13:47:30: 15000000 INFO @ Thu, 05 Dec 2019 13:47:32: 11000000 INFO @ Thu, 05 Dec 2019 13:47:34: 6000000 INFO @ Thu, 05 Dec 2019 13:47:37: 16000000 INFO @ Thu, 05 Dec 2019 13:47:38: 12000000 INFO @ Thu, 05 Dec 2019 13:47:40: 7000000 INFO @ Thu, 05 Dec 2019 13:47:43: 17000000 INFO @ Thu, 05 Dec 2019 13:47:44: #1 tag size is determined as 50 bps INFO @ Thu, 05 Dec 2019 13:47:44: #1 tag size = 50 INFO @ Thu, 05 Dec 2019 13:47:44: #1 total tags in treatment: 17094000 INFO @ Thu, 05 Dec 2019 13:47:44: #1 user defined the maximum tags... INFO @ Thu, 05 Dec 2019 13:47:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Dec 2019 13:47:44: #1 tags after filtering in treatment: 17094000 INFO @ Thu, 05 Dec 2019 13:47:44: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Dec 2019 13:47:44: #1 finished! INFO @ Thu, 05 Dec 2019 13:47:44: #2 Build Peak Model... INFO @ Thu, 05 Dec 2019 13:47:44: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Dec 2019 13:47:44: 13000000 INFO @ Thu, 05 Dec 2019 13:47:45: #2 number of paired peaks: 6 WARNING @ Thu, 05 Dec 2019 13:47:45: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Dec 2019 13:47:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6468498/SRX6468498.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468498/SRX6468498.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468498/SRX6468498.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468498/SRX6468498.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 05 Dec 2019 13:47:46: 8000000 INFO @ Thu, 05 Dec 2019 13:47:50: 14000000 INFO @ Thu, 05 Dec 2019 13:47:52: 9000000 INFO @ Thu, 05 Dec 2019 13:47:56: 15000000 INFO @ Thu, 05 Dec 2019 13:47:58: 10000000 INFO @ Thu, 05 Dec 2019 13:48:02: 16000000 INFO @ Thu, 05 Dec 2019 13:48:03: 11000000 INFO @ Thu, 05 Dec 2019 13:48:08: 17000000 INFO @ Thu, 05 Dec 2019 13:48:09: #1 tag size is determined as 50 bps INFO @ Thu, 05 Dec 2019 13:48:09: #1 tag size = 50 INFO @ Thu, 05 Dec 2019 13:48:09: #1 total tags in treatment: 17094000 INFO @ Thu, 05 Dec 2019 13:48:09: #1 user defined the maximum tags... INFO @ Thu, 05 Dec 2019 13:48:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Dec 2019 13:48:09: #1 tags after filtering in treatment: 17094000 INFO @ Thu, 05 Dec 2019 13:48:09: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Dec 2019 13:48:09: #1 finished! INFO @ Thu, 05 Dec 2019 13:48:09: #2 Build Peak Model... INFO @ Thu, 05 Dec 2019 13:48:09: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Dec 2019 13:48:09: 12000000 INFO @ Thu, 05 Dec 2019 13:48:10: #2 number of paired peaks: 6 WARNING @ Thu, 05 Dec 2019 13:48:10: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Dec 2019 13:48:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6468498/SRX6468498.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468498/SRX6468498.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468498/SRX6468498.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468498/SRX6468498.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 05 Dec 2019 13:48:14: 13000000 INFO @ Thu, 05 Dec 2019 13:48:20: 14000000 INFO @ Thu, 05 Dec 2019 13:48:25: 15000000 INFO @ Thu, 05 Dec 2019 13:48:30: 16000000 INFO @ Thu, 05 Dec 2019 13:48:36: 17000000 INFO @ Thu, 05 Dec 2019 13:48:36: #1 tag size is determined as 50 bps INFO @ Thu, 05 Dec 2019 13:48:36: #1 tag size = 50 INFO @ Thu, 05 Dec 2019 13:48:36: #1 total tags in treatment: 17094000 INFO @ Thu, 05 Dec 2019 13:48:36: #1 user defined the maximum tags... INFO @ Thu, 05 Dec 2019 13:48:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Dec 2019 13:48:37: #1 tags after filtering in treatment: 17094000 INFO @ Thu, 05 Dec 2019 13:48:37: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Dec 2019 13:48:37: #1 finished! INFO @ Thu, 05 Dec 2019 13:48:37: #2 Build Peak Model... INFO @ Thu, 05 Dec 2019 13:48:37: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Dec 2019 13:48:38: #2 number of paired peaks: 6 WARNING @ Thu, 05 Dec 2019 13:48:38: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Dec 2019 13:48:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6468498/SRX6468498.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468498/SRX6468498.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468498/SRX6468498.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468498/SRX6468498.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。