Job ID = 6528446 SRX = SRX6468497 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T15:02:29 prefetch.2.10.7: 1) Downloading 'SRR9710583'... 2020-06-29T15:02:29 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:07:07 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:07:07 prefetch.2.10.7: 1) 'SRR9710583' was downloaded successfully Read 32051351 spots for SRR9710583/SRR9710583.sra Written 32051351 spots for SRR9710583/SRR9710583.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:56 32051351 reads; of these: 32051351 (100.00%) were unpaired; of these: 8603028 (26.84%) aligned 0 times 16220461 (50.61%) aligned exactly 1 time 7227862 (22.55%) aligned >1 times 73.16% overall alignment rate Time searching: 00:09:56 Overall time: 00:09:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 10707459 / 23448323 = 0.4566 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:35:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6468497/SRX6468497.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6468497/SRX6468497.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6468497/SRX6468497.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6468497/SRX6468497.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:35:16: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:35:16: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:35:24: 1000000 INFO @ Tue, 30 Jun 2020 00:35:31: 2000000 INFO @ Tue, 30 Jun 2020 00:35:39: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:35:46: 4000000 INFO @ Tue, 30 Jun 2020 00:35:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6468497/SRX6468497.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6468497/SRX6468497.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6468497/SRX6468497.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6468497/SRX6468497.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:35:46: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:35:46: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:35:53: 1000000 INFO @ Tue, 30 Jun 2020 00:35:53: 5000000 INFO @ Tue, 30 Jun 2020 00:36:00: 2000000 INFO @ Tue, 30 Jun 2020 00:36:01: 6000000 INFO @ Tue, 30 Jun 2020 00:36:07: 3000000 INFO @ Tue, 30 Jun 2020 00:36:09: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:36:14: 4000000 INFO @ Tue, 30 Jun 2020 00:36:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6468497/SRX6468497.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6468497/SRX6468497.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6468497/SRX6468497.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6468497/SRX6468497.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:36:16: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:36:16: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:36:17: 8000000 INFO @ Tue, 30 Jun 2020 00:36:22: 5000000 INFO @ Tue, 30 Jun 2020 00:36:25: 1000000 INFO @ Tue, 30 Jun 2020 00:36:25: 9000000 INFO @ Tue, 30 Jun 2020 00:36:29: 6000000 INFO @ Tue, 30 Jun 2020 00:36:33: 2000000 INFO @ Tue, 30 Jun 2020 00:36:33: 10000000 INFO @ Tue, 30 Jun 2020 00:36:36: 7000000 INFO @ Tue, 30 Jun 2020 00:36:41: 3000000 INFO @ Tue, 30 Jun 2020 00:36:42: 11000000 INFO @ Tue, 30 Jun 2020 00:36:44: 8000000 INFO @ Tue, 30 Jun 2020 00:36:49: 4000000 INFO @ Tue, 30 Jun 2020 00:36:50: 12000000 INFO @ Tue, 30 Jun 2020 00:36:51: 9000000 INFO @ Tue, 30 Jun 2020 00:36:56: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:36:56: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:36:56: #1 total tags in treatment: 12740864 INFO @ Tue, 30 Jun 2020 00:36:56: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:36:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:36:56: #1 tags after filtering in treatment: 12740864 INFO @ Tue, 30 Jun 2020 00:36:56: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:36:56: #1 finished! INFO @ Tue, 30 Jun 2020 00:36:56: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:36:56: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:36:57: #2 number of paired peaks: 58 WARNING @ Tue, 30 Jun 2020 00:36:57: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:36:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6468497/SRX6468497.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468497/SRX6468497.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468497/SRX6468497.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468497/SRX6468497.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:36:57: 5000000 INFO @ Tue, 30 Jun 2020 00:36:58: 10000000 INFO @ Tue, 30 Jun 2020 00:37:05: 6000000 INFO @ Tue, 30 Jun 2020 00:37:05: 11000000 INFO @ Tue, 30 Jun 2020 00:37:13: 7000000 INFO @ Tue, 30 Jun 2020 00:37:13: 12000000 INFO @ Tue, 30 Jun 2020 00:37:18: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:37:18: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:37:18: #1 total tags in treatment: 12740864 INFO @ Tue, 30 Jun 2020 00:37:18: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:37:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:37:18: #1 tags after filtering in treatment: 12740864 INFO @ Tue, 30 Jun 2020 00:37:18: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:37:18: #1 finished! INFO @ Tue, 30 Jun 2020 00:37:18: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:37:18: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:37:19: #2 number of paired peaks: 58 WARNING @ Tue, 30 Jun 2020 00:37:19: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:37:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6468497/SRX6468497.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468497/SRX6468497.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468497/SRX6468497.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468497/SRX6468497.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:37:20: 8000000 INFO @ Tue, 30 Jun 2020 00:37:27: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:37:34: 10000000 INFO @ Tue, 30 Jun 2020 00:37:41: 11000000 INFO @ Tue, 30 Jun 2020 00:37:47: 12000000 INFO @ Tue, 30 Jun 2020 00:37:52: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:37:52: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:37:52: #1 total tags in treatment: 12740864 INFO @ Tue, 30 Jun 2020 00:37:52: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:37:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:37:52: #1 tags after filtering in treatment: 12740864 INFO @ Tue, 30 Jun 2020 00:37:52: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:37:52: #1 finished! INFO @ Tue, 30 Jun 2020 00:37:52: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:37:52: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:37:53: #2 number of paired peaks: 58 WARNING @ Tue, 30 Jun 2020 00:37:53: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:37:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6468497/SRX6468497.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468497/SRX6468497.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468497/SRX6468497.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468497/SRX6468497.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。