Job ID = 6528445 SRX = SRX6468496 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T15:00:28 prefetch.2.10.7: 1) Downloading 'SRR9710582'... 2020-06-29T15:00:28 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:08:41 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:08:41 prefetch.2.10.7: 1) 'SRR9710582' was downloaded successfully Read 32288188 spots for SRR9710582/SRR9710582.sra Written 32288188 spots for SRR9710582/SRR9710582.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:05 32288188 reads; of these: 32288188 (100.00%) were unpaired; of these: 3223181 (9.98%) aligned 0 times 20064419 (62.14%) aligned exactly 1 time 9000588 (27.88%) aligned >1 times 90.02% overall alignment rate Time searching: 00:11:05 Overall time: 00:11:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 9830080 / 29065007 = 0.3382 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:40:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6468496/SRX6468496.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6468496/SRX6468496.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6468496/SRX6468496.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6468496/SRX6468496.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:40:06: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:40:06: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:40:12: 1000000 INFO @ Tue, 30 Jun 2020 00:40:17: 2000000 INFO @ Tue, 30 Jun 2020 00:40:23: 3000000 INFO @ Tue, 30 Jun 2020 00:40:29: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:40:34: 5000000 INFO @ Tue, 30 Jun 2020 00:40:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6468496/SRX6468496.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6468496/SRX6468496.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6468496/SRX6468496.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6468496/SRX6468496.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:40:36: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:40:36: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:40:41: 6000000 INFO @ Tue, 30 Jun 2020 00:40:43: 1000000 INFO @ Tue, 30 Jun 2020 00:40:47: 7000000 INFO @ Tue, 30 Jun 2020 00:40:49: 2000000 INFO @ Tue, 30 Jun 2020 00:40:53: 8000000 INFO @ Tue, 30 Jun 2020 00:40:55: 3000000 INFO @ Tue, 30 Jun 2020 00:40:59: 9000000 INFO @ Tue, 30 Jun 2020 00:41:02: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:41:05: 10000000 INFO @ Tue, 30 Jun 2020 00:41:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6468496/SRX6468496.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6468496/SRX6468496.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6468496/SRX6468496.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6468496/SRX6468496.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:41:06: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:41:06: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:41:08: 5000000 INFO @ Tue, 30 Jun 2020 00:41:12: 11000000 INFO @ Tue, 30 Jun 2020 00:41:12: 1000000 INFO @ Tue, 30 Jun 2020 00:41:14: 6000000 INFO @ Tue, 30 Jun 2020 00:41:18: 12000000 INFO @ Tue, 30 Jun 2020 00:41:18: 2000000 INFO @ Tue, 30 Jun 2020 00:41:21: 7000000 INFO @ Tue, 30 Jun 2020 00:41:24: 13000000 INFO @ Tue, 30 Jun 2020 00:41:25: 3000000 INFO @ Tue, 30 Jun 2020 00:41:28: 8000000 INFO @ Tue, 30 Jun 2020 00:41:31: 4000000 INFO @ Tue, 30 Jun 2020 00:41:31: 14000000 INFO @ Tue, 30 Jun 2020 00:41:34: 9000000 INFO @ Tue, 30 Jun 2020 00:41:37: 5000000 INFO @ Tue, 30 Jun 2020 00:41:37: 15000000 INFO @ Tue, 30 Jun 2020 00:41:40: 10000000 INFO @ Tue, 30 Jun 2020 00:41:43: 6000000 INFO @ Tue, 30 Jun 2020 00:41:44: 16000000 INFO @ Tue, 30 Jun 2020 00:41:47: 11000000 INFO @ Tue, 30 Jun 2020 00:41:49: 7000000 INFO @ Tue, 30 Jun 2020 00:41:50: 17000000 INFO @ Tue, 30 Jun 2020 00:41:53: 12000000 INFO @ Tue, 30 Jun 2020 00:41:56: 8000000 INFO @ Tue, 30 Jun 2020 00:41:56: 18000000 INFO @ Tue, 30 Jun 2020 00:42:00: 13000000 INFO @ Tue, 30 Jun 2020 00:42:02: 9000000 INFO @ Tue, 30 Jun 2020 00:42:03: 19000000 INFO @ Tue, 30 Jun 2020 00:42:04: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:42:04: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:42:04: #1 total tags in treatment: 19234927 INFO @ Tue, 30 Jun 2020 00:42:04: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:42:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:42:05: #1 tags after filtering in treatment: 19234927 INFO @ Tue, 30 Jun 2020 00:42:05: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:42:05: #1 finished! INFO @ Tue, 30 Jun 2020 00:42:05: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:42:05: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:42:06: #2 number of paired peaks: 7 WARNING @ Tue, 30 Jun 2020 00:42:06: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:42:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6468496/SRX6468496.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468496/SRX6468496.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468496/SRX6468496.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468496/SRX6468496.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:42:06: 14000000 INFO @ Tue, 30 Jun 2020 00:42:08: 10000000 INFO @ Tue, 30 Jun 2020 00:42:12: 15000000 INFO @ Tue, 30 Jun 2020 00:42:14: 11000000 INFO @ Tue, 30 Jun 2020 00:42:18: 16000000 INFO @ Tue, 30 Jun 2020 00:42:20: 12000000 INFO @ Tue, 30 Jun 2020 00:42:24: 17000000 INFO @ Tue, 30 Jun 2020 00:42:28: 13000000 INFO @ Tue, 30 Jun 2020 00:42:30: 18000000 INFO @ Tue, 30 Jun 2020 00:42:35: 14000000 INFO @ Tue, 30 Jun 2020 00:42:37: 19000000 INFO @ Tue, 30 Jun 2020 00:42:38: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:42:38: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:42:38: #1 total tags in treatment: 19234927 INFO @ Tue, 30 Jun 2020 00:42:38: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:42:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:42:39: #1 tags after filtering in treatment: 19234927 INFO @ Tue, 30 Jun 2020 00:42:39: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:42:39: #1 finished! INFO @ Tue, 30 Jun 2020 00:42:39: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:42:39: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:42:40: #2 number of paired peaks: 7 WARNING @ Tue, 30 Jun 2020 00:42:40: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:42:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6468496/SRX6468496.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468496/SRX6468496.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468496/SRX6468496.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468496/SRX6468496.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:42:41: 15000000 INFO @ Tue, 30 Jun 2020 00:42:47: 16000000 INFO @ Tue, 30 Jun 2020 00:42:53: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:43:00: 18000000 INFO @ Tue, 30 Jun 2020 00:43:07: 19000000 INFO @ Tue, 30 Jun 2020 00:43:09: #1 tag size is determined as 50 bps INFO @ Tue, 30 Jun 2020 00:43:09: #1 tag size = 50 INFO @ Tue, 30 Jun 2020 00:43:09: #1 total tags in treatment: 19234927 INFO @ Tue, 30 Jun 2020 00:43:09: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:43:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:43:10: #1 tags after filtering in treatment: 19234927 INFO @ Tue, 30 Jun 2020 00:43:10: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:43:10: #1 finished! INFO @ Tue, 30 Jun 2020 00:43:10: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:43:10: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:43:11: #2 number of paired peaks: 7 WARNING @ Tue, 30 Jun 2020 00:43:11: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:43:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6468496/SRX6468496.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468496/SRX6468496.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468496/SRX6468496.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468496/SRX6468496.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。