Job ID = 4178620 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 30,070,766 reads read : 30,070,766 reads written : 30,070,766 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:38 30070766 reads; of these: 30070766 (100.00%) were unpaired; of these: 1239763 (4.12%) aligned 0 times 19556494 (65.03%) aligned exactly 1 time 9274509 (30.84%) aligned >1 times 95.88% overall alignment rate Time searching: 00:11:38 Overall time: 00:11:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 7674553 / 28831003 = 0.2662 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Thu, 05 Dec 2019 13:51:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6468494/SRX6468494.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6468494/SRX6468494.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6468494/SRX6468494.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6468494/SRX6468494.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Dec 2019 13:51:51: #1 read tag files... INFO @ Thu, 05 Dec 2019 13:51:51: #1 read treatment tags... INFO @ Thu, 05 Dec 2019 13:51:57: 1000000 INFO @ Thu, 05 Dec 2019 13:52:04: 2000000 INFO @ Thu, 05 Dec 2019 13:52:11: 3000000 INFO @ Thu, 05 Dec 2019 13:52:18: 4000000 INFO @ Thu, 05 Dec 2019 13:52:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6468494/SRX6468494.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6468494/SRX6468494.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6468494/SRX6468494.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6468494/SRX6468494.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Dec 2019 13:52:21: #1 read tag files... INFO @ Thu, 05 Dec 2019 13:52:21: #1 read treatment tags... INFO @ Thu, 05 Dec 2019 13:52:25: 5000000 INFO @ Thu, 05 Dec 2019 13:52:28: 1000000 INFO @ Thu, 05 Dec 2019 13:52:32: 6000000 INFO @ Thu, 05 Dec 2019 13:52:36: 2000000 INFO @ Thu, 05 Dec 2019 13:52:40: 7000000 INFO @ Thu, 05 Dec 2019 13:52:43: 3000000 INFO @ Thu, 05 Dec 2019 13:52:47: 8000000 BedGraph に変換中... INFO @ Thu, 05 Dec 2019 13:52:51: 4000000 INFO @ Thu, 05 Dec 2019 13:52:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6468494/SRX6468494.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6468494/SRX6468494.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6468494/SRX6468494.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6468494/SRX6468494.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Dec 2019 13:52:51: #1 read tag files... INFO @ Thu, 05 Dec 2019 13:52:51: #1 read treatment tags... INFO @ Thu, 05 Dec 2019 13:52:55: 9000000 INFO @ Thu, 05 Dec 2019 13:52:57: 1000000 INFO @ Thu, 05 Dec 2019 13:52:58: 5000000 INFO @ Thu, 05 Dec 2019 13:53:02: 10000000 INFO @ Thu, 05 Dec 2019 13:53:03: 2000000 INFO @ Thu, 05 Dec 2019 13:53:06: 6000000 INFO @ Thu, 05 Dec 2019 13:53:09: 3000000 INFO @ Thu, 05 Dec 2019 13:53:10: 11000000 INFO @ Thu, 05 Dec 2019 13:53:13: 7000000 INFO @ Thu, 05 Dec 2019 13:53:15: 4000000 INFO @ Thu, 05 Dec 2019 13:53:17: 12000000 INFO @ Thu, 05 Dec 2019 13:53:21: 8000000 INFO @ Thu, 05 Dec 2019 13:53:21: 5000000 INFO @ Thu, 05 Dec 2019 13:53:25: 13000000 INFO @ Thu, 05 Dec 2019 13:53:27: 6000000 INFO @ Thu, 05 Dec 2019 13:53:28: 9000000 INFO @ Thu, 05 Dec 2019 13:53:32: 14000000 INFO @ Thu, 05 Dec 2019 13:53:33: 7000000 INFO @ Thu, 05 Dec 2019 13:53:36: 10000000 INFO @ Thu, 05 Dec 2019 13:53:39: 8000000 INFO @ Thu, 05 Dec 2019 13:53:40: 15000000 INFO @ Thu, 05 Dec 2019 13:53:43: 11000000 INFO @ Thu, 05 Dec 2019 13:53:45: 9000000 INFO @ Thu, 05 Dec 2019 13:53:47: 16000000 INFO @ Thu, 05 Dec 2019 13:53:51: 12000000 INFO @ Thu, 05 Dec 2019 13:53:51: 10000000 INFO @ Thu, 05 Dec 2019 13:53:55: 17000000 INFO @ Thu, 05 Dec 2019 13:53:57: 11000000 INFO @ Thu, 05 Dec 2019 13:53:58: 13000000 INFO @ Thu, 05 Dec 2019 13:54:02: 18000000 INFO @ Thu, 05 Dec 2019 13:54:03: 12000000 INFO @ Thu, 05 Dec 2019 13:54:06: 14000000 INFO @ Thu, 05 Dec 2019 13:54:10: 13000000 INFO @ Thu, 05 Dec 2019 13:54:10: 19000000 INFO @ Thu, 05 Dec 2019 13:54:13: 15000000 INFO @ Thu, 05 Dec 2019 13:54:16: 14000000 INFO @ Thu, 05 Dec 2019 13:54:17: 20000000 INFO @ Thu, 05 Dec 2019 13:54:21: 16000000 INFO @ Thu, 05 Dec 2019 13:54:22: 15000000 INFO @ Thu, 05 Dec 2019 13:54:25: 21000000 INFO @ Thu, 05 Dec 2019 13:54:26: #1 tag size is determined as 50 bps INFO @ Thu, 05 Dec 2019 13:54:26: #1 tag size = 50 INFO @ Thu, 05 Dec 2019 13:54:26: #1 total tags in treatment: 21156450 INFO @ Thu, 05 Dec 2019 13:54:26: #1 user defined the maximum tags... INFO @ Thu, 05 Dec 2019 13:54:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Dec 2019 13:54:26: #1 tags after filtering in treatment: 21156450 INFO @ Thu, 05 Dec 2019 13:54:26: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Dec 2019 13:54:26: #1 finished! INFO @ Thu, 05 Dec 2019 13:54:26: #2 Build Peak Model... INFO @ Thu, 05 Dec 2019 13:54:26: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Dec 2019 13:54:28: #2 number of paired peaks: 4 WARNING @ Thu, 05 Dec 2019 13:54:28: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Dec 2019 13:54:28: Process for pairing-model is terminated! INFO @ Thu, 05 Dec 2019 13:54:28: 16000000 cut: /home/okishinya/chipatlas/results/dm3/SRX6468494/SRX6468494.05_peaks.narrowPeak: No such file or directory INFO @ Thu, 05 Dec 2019 13:54:28: 17000000 pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468494/SRX6468494.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468494/SRX6468494.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468494/SRX6468494.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 05 Dec 2019 13:54:34: 17000000 INFO @ Thu, 05 Dec 2019 13:54:35: 18000000 INFO @ Thu, 05 Dec 2019 13:54:40: 18000000 INFO @ Thu, 05 Dec 2019 13:54:42: 19000000 INFO @ Thu, 05 Dec 2019 13:54:46: 19000000 INFO @ Thu, 05 Dec 2019 13:54:49: 20000000 INFO @ Thu, 05 Dec 2019 13:54:52: 20000000 INFO @ Thu, 05 Dec 2019 13:54:57: 21000000 INFO @ Thu, 05 Dec 2019 13:54:58: #1 tag size is determined as 50 bps INFO @ Thu, 05 Dec 2019 13:54:58: #1 tag size = 50 INFO @ Thu, 05 Dec 2019 13:54:58: #1 total tags in treatment: 21156450 INFO @ Thu, 05 Dec 2019 13:54:58: #1 user defined the maximum tags... INFO @ Thu, 05 Dec 2019 13:54:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Dec 2019 13:54:58: #1 tags after filtering in treatment: 21156450 INFO @ Thu, 05 Dec 2019 13:54:58: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Dec 2019 13:54:58: #1 finished! INFO @ Thu, 05 Dec 2019 13:54:58: #2 Build Peak Model... INFO @ Thu, 05 Dec 2019 13:54:58: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Dec 2019 13:54:58: 21000000 INFO @ Thu, 05 Dec 2019 13:54:59: #2 number of paired peaks: 4 WARNING @ Thu, 05 Dec 2019 13:54:59: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Dec 2019 13:54:59: Process for pairing-model is terminated! INFO @ Thu, 05 Dec 2019 13:54:59: #1 tag size is determined as 50 bps INFO @ Thu, 05 Dec 2019 13:54:59: #1 tag size = 50 INFO @ Thu, 05 Dec 2019 13:54:59: #1 total tags in treatment: 21156450 INFO @ Thu, 05 Dec 2019 13:54:59: #1 user defined the maximum tags... INFO @ Thu, 05 Dec 2019 13:54:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) cut: /home/okishinya/chipatlas/results/dm3/SRX6468494/SRX6468494.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468494/SRX6468494.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468494/SRX6468494.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468494/SRX6468494.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 05 Dec 2019 13:55:00: #1 tags after filtering in treatment: 21156450 INFO @ Thu, 05 Dec 2019 13:55:00: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Dec 2019 13:55:00: #1 finished! INFO @ Thu, 05 Dec 2019 13:55:00: #2 Build Peak Model... INFO @ Thu, 05 Dec 2019 13:55:00: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Dec 2019 13:55:01: #2 number of paired peaks: 4 WARNING @ Thu, 05 Dec 2019 13:55:01: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Dec 2019 13:55:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6468494/SRX6468494.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468494/SRX6468494.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468494/SRX6468494.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468494/SRX6468494.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。