Job ID = 4178616 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 28,868,123 reads read : 28,868,123 reads written : 28,868,123 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:23 28868123 reads; of these: 28868123 (100.00%) were unpaired; of these: 1602213 (5.55%) aligned 0 times 20838000 (72.18%) aligned exactly 1 time 6427910 (22.27%) aligned >1 times 94.45% overall alignment rate Time searching: 00:10:23 Overall time: 00:10:23 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 9975981 / 27265910 = 0.3659 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Thu, 05 Dec 2019 13:49:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6468490/SRX6468490.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6468490/SRX6468490.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6468490/SRX6468490.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6468490/SRX6468490.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Dec 2019 13:49:01: #1 read tag files... INFO @ Thu, 05 Dec 2019 13:49:01: #1 read treatment tags... INFO @ Thu, 05 Dec 2019 13:49:09: 1000000 INFO @ Thu, 05 Dec 2019 13:49:17: 2000000 INFO @ Thu, 05 Dec 2019 13:49:26: 3000000 INFO @ Thu, 05 Dec 2019 13:49:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6468490/SRX6468490.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6468490/SRX6468490.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6468490/SRX6468490.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6468490/SRX6468490.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Dec 2019 13:49:30: #1 read tag files... INFO @ Thu, 05 Dec 2019 13:49:30: #1 read treatment tags... INFO @ Thu, 05 Dec 2019 13:49:34: 4000000 INFO @ Thu, 05 Dec 2019 13:49:39: 1000000 INFO @ Thu, 05 Dec 2019 13:49:43: 5000000 INFO @ Thu, 05 Dec 2019 13:49:48: 2000000 INFO @ Thu, 05 Dec 2019 13:49:51: 6000000 INFO @ Thu, 05 Dec 2019 13:49:57: 3000000 BedGraph に変換中... INFO @ Thu, 05 Dec 2019 13:50:00: 7000000 INFO @ Thu, 05 Dec 2019 13:50:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6468490/SRX6468490.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6468490/SRX6468490.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6468490/SRX6468490.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6468490/SRX6468490.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Dec 2019 13:50:00: #1 read tag files... INFO @ Thu, 05 Dec 2019 13:50:00: #1 read treatment tags... INFO @ Thu, 05 Dec 2019 13:50:06: 4000000 INFO @ Thu, 05 Dec 2019 13:50:08: 1000000 INFO @ Thu, 05 Dec 2019 13:50:09: 8000000 INFO @ Thu, 05 Dec 2019 13:50:15: 5000000 INFO @ Thu, 05 Dec 2019 13:50:15: 2000000 INFO @ Thu, 05 Dec 2019 13:50:19: 9000000 INFO @ Thu, 05 Dec 2019 13:50:23: 3000000 INFO @ Thu, 05 Dec 2019 13:50:24: 6000000 INFO @ Thu, 05 Dec 2019 13:50:28: 10000000 INFO @ Thu, 05 Dec 2019 13:50:31: 4000000 INFO @ Thu, 05 Dec 2019 13:50:33: 7000000 INFO @ Thu, 05 Dec 2019 13:50:37: 11000000 INFO @ Thu, 05 Dec 2019 13:50:38: 5000000 INFO @ Thu, 05 Dec 2019 13:50:42: 8000000 INFO @ Thu, 05 Dec 2019 13:50:46: 6000000 INFO @ Thu, 05 Dec 2019 13:50:47: 12000000 INFO @ Thu, 05 Dec 2019 13:50:52: 9000000 INFO @ Thu, 05 Dec 2019 13:50:54: 7000000 INFO @ Thu, 05 Dec 2019 13:50:56: 13000000 INFO @ Thu, 05 Dec 2019 13:51:01: 10000000 INFO @ Thu, 05 Dec 2019 13:51:02: 8000000 INFO @ Thu, 05 Dec 2019 13:51:04: 14000000 INFO @ Thu, 05 Dec 2019 13:51:10: 11000000 INFO @ Thu, 05 Dec 2019 13:51:10: 9000000 INFO @ Thu, 05 Dec 2019 13:51:12: 15000000 INFO @ Thu, 05 Dec 2019 13:51:17: 10000000 INFO @ Thu, 05 Dec 2019 13:51:18: 12000000 INFO @ Thu, 05 Dec 2019 13:51:20: 16000000 INFO @ Thu, 05 Dec 2019 13:51:25: 11000000 INFO @ Thu, 05 Dec 2019 13:51:28: 13000000 INFO @ Thu, 05 Dec 2019 13:51:29: 17000000 INFO @ Thu, 05 Dec 2019 13:51:32: #1 tag size is determined as 51 bps INFO @ Thu, 05 Dec 2019 13:51:32: #1 tag size = 51 INFO @ Thu, 05 Dec 2019 13:51:32: #1 total tags in treatment: 17289929 INFO @ Thu, 05 Dec 2019 13:51:32: #1 user defined the maximum tags... INFO @ Thu, 05 Dec 2019 13:51:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Dec 2019 13:51:32: #1 tags after filtering in treatment: 17289929 INFO @ Thu, 05 Dec 2019 13:51:32: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Dec 2019 13:51:32: #1 finished! INFO @ Thu, 05 Dec 2019 13:51:32: #2 Build Peak Model... INFO @ Thu, 05 Dec 2019 13:51:32: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Dec 2019 13:51:32: 12000000 INFO @ Thu, 05 Dec 2019 13:51:34: #2 number of paired peaks: 4 WARNING @ Thu, 05 Dec 2019 13:51:34: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Dec 2019 13:51:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6468490/SRX6468490.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468490/SRX6468490.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468490/SRX6468490.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468490/SRX6468490.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 05 Dec 2019 13:51:36: 14000000 INFO @ Thu, 05 Dec 2019 13:51:40: 13000000 INFO @ Thu, 05 Dec 2019 13:51:45: 15000000 INFO @ Thu, 05 Dec 2019 13:51:48: 14000000 INFO @ Thu, 05 Dec 2019 13:51:54: 16000000 INFO @ Thu, 05 Dec 2019 13:51:55: 15000000 INFO @ Thu, 05 Dec 2019 13:52:04: 16000000 INFO @ Thu, 05 Dec 2019 13:52:05: 17000000 INFO @ Thu, 05 Dec 2019 13:52:11: #1 tag size is determined as 51 bps INFO @ Thu, 05 Dec 2019 13:52:11: #1 tag size = 51 INFO @ Thu, 05 Dec 2019 13:52:11: #1 total tags in treatment: 17289929 INFO @ Thu, 05 Dec 2019 13:52:11: #1 user defined the maximum tags... INFO @ Thu, 05 Dec 2019 13:52:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Dec 2019 13:52:12: #1 tags after filtering in treatment: 17289929 INFO @ Thu, 05 Dec 2019 13:52:12: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Dec 2019 13:52:12: #1 finished! INFO @ Thu, 05 Dec 2019 13:52:12: #2 Build Peak Model... INFO @ Thu, 05 Dec 2019 13:52:12: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Dec 2019 13:52:14: #2 number of paired peaks: 4 WARNING @ Thu, 05 Dec 2019 13:52:14: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Dec 2019 13:52:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6468490/SRX6468490.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 9 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468490/SRX6468490.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468490/SRX6468490.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468490/SRX6468490.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 05 Dec 2019 13:52:19: 17000000 INFO @ Thu, 05 Dec 2019 13:52:21: #1 tag size is determined as 51 bps INFO @ Thu, 05 Dec 2019 13:52:21: #1 tag size = 51 INFO @ Thu, 05 Dec 2019 13:52:21: #1 total tags in treatment: 17289929 INFO @ Thu, 05 Dec 2019 13:52:21: #1 user defined the maximum tags... INFO @ Thu, 05 Dec 2019 13:52:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Dec 2019 13:52:22: #1 tags after filtering in treatment: 17289929 INFO @ Thu, 05 Dec 2019 13:52:22: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Dec 2019 13:52:22: #1 finished! INFO @ Thu, 05 Dec 2019 13:52:22: #2 Build Peak Model... INFO @ Thu, 05 Dec 2019 13:52:22: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Dec 2019 13:52:23: #2 number of paired peaks: 4 WARNING @ Thu, 05 Dec 2019 13:52:23: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Dec 2019 13:52:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6468490/SRX6468490.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468490/SRX6468490.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468490/SRX6468490.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468490/SRX6468490.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。