Job ID = 4178615 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 35,317,384 reads read : 35,317,384 reads written : 35,317,384 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:32 35317384 reads; of these: 35317384 (100.00%) were unpaired; of these: 2488805 (7.05%) aligned 0 times 24888485 (70.47%) aligned exactly 1 time 7940094 (22.48%) aligned >1 times 92.95% overall alignment rate Time searching: 00:12:32 Overall time: 00:12:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 15320031 / 32828579 = 0.4667 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Thu, 05 Dec 2019 13:54:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6468489/SRX6468489.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6468489/SRX6468489.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6468489/SRX6468489.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6468489/SRX6468489.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Dec 2019 13:54:23: #1 read tag files... INFO @ Thu, 05 Dec 2019 13:54:23: #1 read treatment tags... INFO @ Thu, 05 Dec 2019 13:54:31: 1000000 INFO @ Thu, 05 Dec 2019 13:54:41: 2000000 INFO @ Thu, 05 Dec 2019 13:54:49: 3000000 INFO @ Thu, 05 Dec 2019 13:54:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6468489/SRX6468489.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6468489/SRX6468489.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6468489/SRX6468489.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6468489/SRX6468489.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Dec 2019 13:54:53: #1 read tag files... INFO @ Thu, 05 Dec 2019 13:54:53: #1 read treatment tags... INFO @ Thu, 05 Dec 2019 13:54:56: 4000000 INFO @ Thu, 05 Dec 2019 13:55:02: 1000000 INFO @ Thu, 05 Dec 2019 13:55:03: 5000000 INFO @ Thu, 05 Dec 2019 13:55:10: 2000000 INFO @ Thu, 05 Dec 2019 13:55:11: 6000000 INFO @ Thu, 05 Dec 2019 13:55:18: 3000000 INFO @ Thu, 05 Dec 2019 13:55:18: 7000000 BedGraph に変換中... INFO @ Thu, 05 Dec 2019 13:55:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6468489/SRX6468489.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6468489/SRX6468489.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6468489/SRX6468489.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6468489/SRX6468489.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Dec 2019 13:55:24: #1 read tag files... INFO @ Thu, 05 Dec 2019 13:55:24: #1 read treatment tags... INFO @ Thu, 05 Dec 2019 13:55:26: 8000000 INFO @ Thu, 05 Dec 2019 13:55:26: 4000000 INFO @ Thu, 05 Dec 2019 13:55:33: 9000000 INFO @ Thu, 05 Dec 2019 13:55:34: 5000000 INFO @ Thu, 05 Dec 2019 13:55:35: 1000000 INFO @ Thu, 05 Dec 2019 13:55:41: 10000000 INFO @ Thu, 05 Dec 2019 13:55:43: 6000000 INFO @ Thu, 05 Dec 2019 13:55:46: 2000000 INFO @ Thu, 05 Dec 2019 13:55:48: 11000000 INFO @ Thu, 05 Dec 2019 13:55:52: 7000000 INFO @ Thu, 05 Dec 2019 13:55:56: 3000000 INFO @ Thu, 05 Dec 2019 13:55:56: 12000000 INFO @ Thu, 05 Dec 2019 13:56:00: 8000000 INFO @ Thu, 05 Dec 2019 13:56:04: 13000000 INFO @ Thu, 05 Dec 2019 13:56:05: 4000000 INFO @ Thu, 05 Dec 2019 13:56:08: 9000000 INFO @ Thu, 05 Dec 2019 13:56:11: 14000000 INFO @ Thu, 05 Dec 2019 13:56:15: 5000000 INFO @ Thu, 05 Dec 2019 13:56:18: 10000000 INFO @ Thu, 05 Dec 2019 13:56:20: 15000000 INFO @ Thu, 05 Dec 2019 13:56:26: 6000000 INFO @ Thu, 05 Dec 2019 13:56:27: 11000000 INFO @ Thu, 05 Dec 2019 13:56:30: 16000000 INFO @ Thu, 05 Dec 2019 13:56:37: 12000000 INFO @ Thu, 05 Dec 2019 13:56:39: 17000000 INFO @ Thu, 05 Dec 2019 13:56:40: 7000000 INFO @ Thu, 05 Dec 2019 13:56:43: #1 tag size is determined as 51 bps INFO @ Thu, 05 Dec 2019 13:56:43: #1 tag size = 51 INFO @ Thu, 05 Dec 2019 13:56:43: #1 total tags in treatment: 17508548 INFO @ Thu, 05 Dec 2019 13:56:43: #1 user defined the maximum tags... INFO @ Thu, 05 Dec 2019 13:56:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Dec 2019 13:56:44: #1 tags after filtering in treatment: 17508548 INFO @ Thu, 05 Dec 2019 13:56:44: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Dec 2019 13:56:44: #1 finished! INFO @ Thu, 05 Dec 2019 13:56:44: #2 Build Peak Model... INFO @ Thu, 05 Dec 2019 13:56:44: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Dec 2019 13:56:45: 13000000 INFO @ Thu, 05 Dec 2019 13:56:45: #2 number of paired peaks: 0 WARNING @ Thu, 05 Dec 2019 13:56:45: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Dec 2019 13:56:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6468489/SRX6468489.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468489/SRX6468489.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468489/SRX6468489.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468489/SRX6468489.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 05 Dec 2019 13:56:51: 8000000 INFO @ Thu, 05 Dec 2019 13:56:53: 14000000 INFO @ Thu, 05 Dec 2019 13:57:02: 15000000 INFO @ Thu, 05 Dec 2019 13:57:03: 9000000 INFO @ Thu, 05 Dec 2019 13:57:10: 16000000 INFO @ Thu, 05 Dec 2019 13:57:15: 10000000 INFO @ Thu, 05 Dec 2019 13:57:18: 17000000 INFO @ Thu, 05 Dec 2019 13:57:22: #1 tag size is determined as 51 bps INFO @ Thu, 05 Dec 2019 13:57:22: #1 tag size = 51 INFO @ Thu, 05 Dec 2019 13:57:22: #1 total tags in treatment: 17508548 INFO @ Thu, 05 Dec 2019 13:57:22: #1 user defined the maximum tags... INFO @ Thu, 05 Dec 2019 13:57:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Dec 2019 13:57:23: #1 tags after filtering in treatment: 17508548 INFO @ Thu, 05 Dec 2019 13:57:23: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Dec 2019 13:57:23: #1 finished! INFO @ Thu, 05 Dec 2019 13:57:23: #2 Build Peak Model... INFO @ Thu, 05 Dec 2019 13:57:23: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Dec 2019 13:57:24: #2 number of paired peaks: 0 WARNING @ Thu, 05 Dec 2019 13:57:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Dec 2019 13:57:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6468489/SRX6468489.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468489/SRX6468489.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468489/SRX6468489.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468489/SRX6468489.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 05 Dec 2019 13:57:26: 11000000 INFO @ Thu, 05 Dec 2019 13:57:37: 12000000 INFO @ Thu, 05 Dec 2019 13:57:48: 13000000 INFO @ Thu, 05 Dec 2019 13:57:59: 14000000 INFO @ Thu, 05 Dec 2019 13:58:11: 15000000 INFO @ Thu, 05 Dec 2019 13:58:22: 16000000 INFO @ Thu, 05 Dec 2019 13:58:33: 17000000 INFO @ Thu, 05 Dec 2019 13:58:38: #1 tag size is determined as 51 bps INFO @ Thu, 05 Dec 2019 13:58:38: #1 tag size = 51 INFO @ Thu, 05 Dec 2019 13:58:38: #1 total tags in treatment: 17508548 INFO @ Thu, 05 Dec 2019 13:58:38: #1 user defined the maximum tags... INFO @ Thu, 05 Dec 2019 13:58:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Dec 2019 13:58:38: #1 tags after filtering in treatment: 17508548 INFO @ Thu, 05 Dec 2019 13:58:38: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Dec 2019 13:58:38: #1 finished! INFO @ Thu, 05 Dec 2019 13:58:38: #2 Build Peak Model... INFO @ Thu, 05 Dec 2019 13:58:38: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Dec 2019 13:58:40: #2 number of paired peaks: 0 WARNING @ Thu, 05 Dec 2019 13:58:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Dec 2019 13:58:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6468489/SRX6468489.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468489/SRX6468489.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468489/SRX6468489.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468489/SRX6468489.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。