Job ID = 4178614 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 31,411,937 reads read : 31,411,937 reads written : 31,411,937 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:12:41 31411937 reads; of these: 31411937 (100.00%) were unpaired; of these: 1812537 (5.77%) aligned 0 times 22271383 (70.90%) aligned exactly 1 time 7328017 (23.33%) aligned >1 times 94.23% overall alignment rate Time searching: 00:12:46 Overall time: 00:12:46 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 11336242 / 29599400 = 0.3830 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Thu, 05 Dec 2019 13:51:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6468488/SRX6468488.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6468488/SRX6468488.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6468488/SRX6468488.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6468488/SRX6468488.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Dec 2019 13:51:42: #1 read tag files... INFO @ Thu, 05 Dec 2019 13:51:42: #1 read treatment tags... INFO @ Thu, 05 Dec 2019 13:51:50: 1000000 INFO @ Thu, 05 Dec 2019 13:51:58: 2000000 INFO @ Thu, 05 Dec 2019 13:52:05: 3000000 INFO @ Thu, 05 Dec 2019 13:52:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6468488/SRX6468488.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6468488/SRX6468488.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6468488/SRX6468488.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6468488/SRX6468488.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Dec 2019 13:52:10: #1 read tag files... INFO @ Thu, 05 Dec 2019 13:52:10: #1 read treatment tags... INFO @ Thu, 05 Dec 2019 13:52:13: 4000000 INFO @ Thu, 05 Dec 2019 13:52:21: 5000000 INFO @ Thu, 05 Dec 2019 13:52:21: 1000000 INFO @ Thu, 05 Dec 2019 13:52:28: 6000000 INFO @ Thu, 05 Dec 2019 13:52:32: 2000000 INFO @ Thu, 05 Dec 2019 13:52:36: 7000000 BedGraph に変換中... INFO @ Thu, 05 Dec 2019 13:52:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6468488/SRX6468488.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6468488/SRX6468488.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6468488/SRX6468488.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6468488/SRX6468488.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Dec 2019 13:52:40: #1 read tag files... INFO @ Thu, 05 Dec 2019 13:52:40: #1 read treatment tags... INFO @ Thu, 05 Dec 2019 13:52:42: 3000000 INFO @ Thu, 05 Dec 2019 13:52:44: 8000000 INFO @ Thu, 05 Dec 2019 13:52:49: 1000000 INFO @ Thu, 05 Dec 2019 13:52:52: 9000000 INFO @ Thu, 05 Dec 2019 13:52:53: 4000000 INFO @ Thu, 05 Dec 2019 13:52:58: 2000000 INFO @ Thu, 05 Dec 2019 13:53:00: 10000000 INFO @ Thu, 05 Dec 2019 13:53:04: 5000000 INFO @ Thu, 05 Dec 2019 13:53:07: 3000000 INFO @ Thu, 05 Dec 2019 13:53:08: 11000000 INFO @ Thu, 05 Dec 2019 13:53:16: 6000000 INFO @ Thu, 05 Dec 2019 13:53:17: 4000000 INFO @ Thu, 05 Dec 2019 13:53:18: 12000000 INFO @ Thu, 05 Dec 2019 13:53:26: 5000000 INFO @ Thu, 05 Dec 2019 13:53:27: 13000000 INFO @ Thu, 05 Dec 2019 13:53:28: 7000000 INFO @ Thu, 05 Dec 2019 13:53:35: 14000000 INFO @ Thu, 05 Dec 2019 13:53:36: 6000000 INFO @ Thu, 05 Dec 2019 13:53:39: 8000000 INFO @ Thu, 05 Dec 2019 13:53:44: 15000000 INFO @ Thu, 05 Dec 2019 13:53:45: 7000000 INFO @ Thu, 05 Dec 2019 13:53:50: 9000000 INFO @ Thu, 05 Dec 2019 13:53:52: 16000000 INFO @ Thu, 05 Dec 2019 13:53:53: 8000000 INFO @ Thu, 05 Dec 2019 13:54:00: 17000000 INFO @ Thu, 05 Dec 2019 13:54:02: 10000000 INFO @ Thu, 05 Dec 2019 13:54:02: 9000000 INFO @ Thu, 05 Dec 2019 13:54:09: 18000000 INFO @ Thu, 05 Dec 2019 13:54:11: #1 tag size is determined as 50 bps INFO @ Thu, 05 Dec 2019 13:54:11: #1 tag size = 50 INFO @ Thu, 05 Dec 2019 13:54:11: #1 total tags in treatment: 18263158 INFO @ Thu, 05 Dec 2019 13:54:11: #1 user defined the maximum tags... INFO @ Thu, 05 Dec 2019 13:54:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Dec 2019 13:54:11: #1 tags after filtering in treatment: 18263158 INFO @ Thu, 05 Dec 2019 13:54:11: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Dec 2019 13:54:11: #1 finished! INFO @ Thu, 05 Dec 2019 13:54:11: #2 Build Peak Model... INFO @ Thu, 05 Dec 2019 13:54:11: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Dec 2019 13:54:11: 10000000 INFO @ Thu, 05 Dec 2019 13:54:13: 11000000 INFO @ Thu, 05 Dec 2019 13:54:13: #2 number of paired peaks: 0 WARNING @ Thu, 05 Dec 2019 13:54:13: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Dec 2019 13:54:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6468488/SRX6468488.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468488/SRX6468488.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468488/SRX6468488.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468488/SRX6468488.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 05 Dec 2019 13:54:20: 11000000 INFO @ Thu, 05 Dec 2019 13:54:24: 12000000 INFO @ Thu, 05 Dec 2019 13:54:29: 12000000 INFO @ Thu, 05 Dec 2019 13:54:38: 13000000 INFO @ Thu, 05 Dec 2019 13:54:41: 13000000 INFO @ Thu, 05 Dec 2019 13:54:49: 14000000 INFO @ Thu, 05 Dec 2019 13:54:51: 14000000 INFO @ Thu, 05 Dec 2019 13:55:00: 15000000 INFO @ Thu, 05 Dec 2019 13:55:00: 15000000 INFO @ Thu, 05 Dec 2019 13:55:09: 16000000 INFO @ Thu, 05 Dec 2019 13:55:12: 16000000 INFO @ Thu, 05 Dec 2019 13:55:18: 17000000 INFO @ Thu, 05 Dec 2019 13:55:23: 17000000 INFO @ Thu, 05 Dec 2019 13:55:27: 18000000 INFO @ Thu, 05 Dec 2019 13:55:29: #1 tag size is determined as 50 bps INFO @ Thu, 05 Dec 2019 13:55:29: #1 tag size = 50 INFO @ Thu, 05 Dec 2019 13:55:29: #1 total tags in treatment: 18263158 INFO @ Thu, 05 Dec 2019 13:55:29: #1 user defined the maximum tags... INFO @ Thu, 05 Dec 2019 13:55:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Dec 2019 13:55:30: #1 tags after filtering in treatment: 18263158 INFO @ Thu, 05 Dec 2019 13:55:30: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Dec 2019 13:55:30: #1 finished! INFO @ Thu, 05 Dec 2019 13:55:30: #2 Build Peak Model... INFO @ Thu, 05 Dec 2019 13:55:30: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Dec 2019 13:55:31: #2 number of paired peaks: 0 WARNING @ Thu, 05 Dec 2019 13:55:31: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Dec 2019 13:55:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6468488/SRX6468488.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468488/SRX6468488.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468488/SRX6468488.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468488/SRX6468488.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 05 Dec 2019 13:55:34: 18000000 INFO @ Thu, 05 Dec 2019 13:55:37: #1 tag size is determined as 50 bps INFO @ Thu, 05 Dec 2019 13:55:37: #1 tag size = 50 INFO @ Thu, 05 Dec 2019 13:55:37: #1 total tags in treatment: 18263158 INFO @ Thu, 05 Dec 2019 13:55:37: #1 user defined the maximum tags... INFO @ Thu, 05 Dec 2019 13:55:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Dec 2019 13:55:37: #1 tags after filtering in treatment: 18263158 INFO @ Thu, 05 Dec 2019 13:55:37: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Dec 2019 13:55:37: #1 finished! INFO @ Thu, 05 Dec 2019 13:55:37: #2 Build Peak Model... INFO @ Thu, 05 Dec 2019 13:55:37: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Dec 2019 13:55:39: #2 number of paired peaks: 0 WARNING @ Thu, 05 Dec 2019 13:55:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Dec 2019 13:55:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6468488/SRX6468488.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468488/SRX6468488.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468488/SRX6468488.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468488/SRX6468488.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。