Job ID = 4178610 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 27,773,923 reads read : 27,773,923 reads written : 27,773,923 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:01 27773923 reads; of these: 27773923 (100.00%) were unpaired; of these: 1633853 (5.88%) aligned 0 times 20529261 (73.92%) aligned exactly 1 time 5610809 (20.20%) aligned >1 times 94.12% overall alignment rate Time searching: 00:10:01 Overall time: 00:10:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 10134676 / 26140070 = 0.3877 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Thu, 05 Dec 2019 13:46:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6468486/SRX6468486.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6468486/SRX6468486.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6468486/SRX6468486.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6468486/SRX6468486.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Dec 2019 13:46:06: #1 read tag files... INFO @ Thu, 05 Dec 2019 13:46:06: #1 read treatment tags... INFO @ Thu, 05 Dec 2019 13:46:18: 1000000 INFO @ Thu, 05 Dec 2019 13:46:30: 2000000 INFO @ Thu, 05 Dec 2019 13:46:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6468486/SRX6468486.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6468486/SRX6468486.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6468486/SRX6468486.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6468486/SRX6468486.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Dec 2019 13:46:36: #1 read tag files... INFO @ Thu, 05 Dec 2019 13:46:36: #1 read treatment tags... INFO @ Thu, 05 Dec 2019 13:46:42: 3000000 INFO @ Thu, 05 Dec 2019 13:46:46: 1000000 INFO @ Thu, 05 Dec 2019 13:46:53: 4000000 INFO @ Thu, 05 Dec 2019 13:46:57: 2000000 BedGraph に変換中... INFO @ Thu, 05 Dec 2019 13:47:05: 5000000 INFO @ Thu, 05 Dec 2019 13:47:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6468486/SRX6468486.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6468486/SRX6468486.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6468486/SRX6468486.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6468486/SRX6468486.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Dec 2019 13:47:06: #1 read tag files... INFO @ Thu, 05 Dec 2019 13:47:06: #1 read treatment tags... INFO @ Thu, 05 Dec 2019 13:47:07: 3000000 INFO @ Thu, 05 Dec 2019 13:47:15: 1000000 INFO @ Thu, 05 Dec 2019 13:47:17: 4000000 INFO @ Thu, 05 Dec 2019 13:47:17: 6000000 INFO @ Thu, 05 Dec 2019 13:47:24: 2000000 INFO @ Thu, 05 Dec 2019 13:47:27: 5000000 INFO @ Thu, 05 Dec 2019 13:47:29: 7000000 INFO @ Thu, 05 Dec 2019 13:47:33: 3000000 INFO @ Thu, 05 Dec 2019 13:47:37: 6000000 INFO @ Thu, 05 Dec 2019 13:47:41: 8000000 INFO @ Thu, 05 Dec 2019 13:47:42: 4000000 INFO @ Thu, 05 Dec 2019 13:47:48: 7000000 INFO @ Thu, 05 Dec 2019 13:47:51: 5000000 INFO @ Thu, 05 Dec 2019 13:47:52: 9000000 INFO @ Thu, 05 Dec 2019 13:47:58: 8000000 INFO @ Thu, 05 Dec 2019 13:48:00: 6000000 INFO @ Thu, 05 Dec 2019 13:48:04: 10000000 INFO @ Thu, 05 Dec 2019 13:48:06: 9000000 INFO @ Thu, 05 Dec 2019 13:48:10: 7000000 INFO @ Thu, 05 Dec 2019 13:48:15: 10000000 INFO @ Thu, 05 Dec 2019 13:48:15: 11000000 INFO @ Thu, 05 Dec 2019 13:48:20: 8000000 INFO @ Thu, 05 Dec 2019 13:48:23: 11000000 INFO @ Thu, 05 Dec 2019 13:48:27: 12000000 INFO @ Thu, 05 Dec 2019 13:48:30: 9000000 INFO @ Thu, 05 Dec 2019 13:48:31: 12000000 INFO @ Thu, 05 Dec 2019 13:48:39: 13000000 INFO @ Thu, 05 Dec 2019 13:48:40: 13000000 INFO @ Thu, 05 Dec 2019 13:48:41: 10000000 INFO @ Thu, 05 Dec 2019 13:48:49: 14000000 INFO @ Thu, 05 Dec 2019 13:48:51: 14000000 INFO @ Thu, 05 Dec 2019 13:48:51: 11000000 INFO @ Thu, 05 Dec 2019 13:48:58: 15000000 INFO @ Thu, 05 Dec 2019 13:49:01: 12000000 INFO @ Thu, 05 Dec 2019 13:49:02: 15000000 INFO @ Thu, 05 Dec 2019 13:49:08: 16000000 INFO @ Thu, 05 Dec 2019 13:49:08: #1 tag size is determined as 50 bps INFO @ Thu, 05 Dec 2019 13:49:08: #1 tag size = 50 INFO @ Thu, 05 Dec 2019 13:49:08: #1 total tags in treatment: 16005394 INFO @ Thu, 05 Dec 2019 13:49:08: #1 user defined the maximum tags... INFO @ Thu, 05 Dec 2019 13:49:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Dec 2019 13:49:08: #1 tags after filtering in treatment: 16005394 INFO @ Thu, 05 Dec 2019 13:49:08: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Dec 2019 13:49:08: #1 finished! INFO @ Thu, 05 Dec 2019 13:49:08: #2 Build Peak Model... INFO @ Thu, 05 Dec 2019 13:49:08: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Dec 2019 13:49:10: #2 number of paired peaks: 2 WARNING @ Thu, 05 Dec 2019 13:49:10: Too few paired peaks (2) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Dec 2019 13:49:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6468486/SRX6468486.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468486/SRX6468486.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468486/SRX6468486.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468486/SRX6468486.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 05 Dec 2019 13:49:12: 13000000 INFO @ Thu, 05 Dec 2019 13:49:14: 16000000 INFO @ Thu, 05 Dec 2019 13:49:14: #1 tag size is determined as 50 bps INFO @ Thu, 05 Dec 2019 13:49:14: #1 tag size = 50 INFO @ Thu, 05 Dec 2019 13:49:14: #1 total tags in treatment: 16005394 INFO @ Thu, 05 Dec 2019 13:49:14: #1 user defined the maximum tags... INFO @ Thu, 05 Dec 2019 13:49:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Dec 2019 13:49:14: #1 tags after filtering in treatment: 16005394 INFO @ Thu, 05 Dec 2019 13:49:14: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Dec 2019 13:49:14: #1 finished! INFO @ Thu, 05 Dec 2019 13:49:14: #2 Build Peak Model... INFO @ Thu, 05 Dec 2019 13:49:14: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Dec 2019 13:49:16: #2 number of paired peaks: 2 WARNING @ Thu, 05 Dec 2019 13:49:16: Too few paired peaks (2) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Dec 2019 13:49:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6468486/SRX6468486.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468486/SRX6468486.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468486/SRX6468486.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468486/SRX6468486.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 05 Dec 2019 13:49:22: 14000000 INFO @ Thu, 05 Dec 2019 13:49:32: 15000000 INFO @ Thu, 05 Dec 2019 13:49:41: 16000000 INFO @ Thu, 05 Dec 2019 13:49:42: #1 tag size is determined as 50 bps INFO @ Thu, 05 Dec 2019 13:49:42: #1 tag size = 50 INFO @ Thu, 05 Dec 2019 13:49:42: #1 total tags in treatment: 16005394 INFO @ Thu, 05 Dec 2019 13:49:42: #1 user defined the maximum tags... INFO @ Thu, 05 Dec 2019 13:49:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Dec 2019 13:49:42: #1 tags after filtering in treatment: 16005394 INFO @ Thu, 05 Dec 2019 13:49:42: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Dec 2019 13:49:42: #1 finished! INFO @ Thu, 05 Dec 2019 13:49:42: #2 Build Peak Model... INFO @ Thu, 05 Dec 2019 13:49:42: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Dec 2019 13:49:44: #2 number of paired peaks: 2 WARNING @ Thu, 05 Dec 2019 13:49:44: Too few paired peaks (2) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Dec 2019 13:49:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6468486/SRX6468486.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468486/SRX6468486.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468486/SRX6468486.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6468486/SRX6468486.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。