Job ID = 6528436 SRX = SRX645133 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T15:17:18 prefetch.2.10.7: 1) Downloading 'SRR1505733'... 2020-06-29T15:17:18 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:30:51 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:30:51 prefetch.2.10.7: 1) 'SRR1505733' was downloaded successfully Read 60000000 spots for SRR1505733/SRR1505733.sra Written 60000000 spots for SRR1505733/SRR1505733.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:19:28 60000000 reads; of these: 60000000 (100.00%) were unpaired; of these: 42874739 (71.46%) aligned 0 times 12536288 (20.89%) aligned exactly 1 time 4588973 (7.65%) aligned >1 times 28.54% overall alignment rate Time searching: 00:19:28 Overall time: 00:19:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1373957 / 17125261 = 0.0802 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:28:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX645133/SRX645133.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX645133/SRX645133.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX645133/SRX645133.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX645133/SRX645133.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:28:45: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:28:45: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:28:55: 1000000 INFO @ Tue, 30 Jun 2020 01:29:05: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:29:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX645133/SRX645133.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX645133/SRX645133.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX645133/SRX645133.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX645133/SRX645133.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:29:15: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:29:15: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:29:15: 3000000 INFO @ Tue, 30 Jun 2020 01:29:24: 1000000 INFO @ Tue, 30 Jun 2020 01:29:26: 4000000 INFO @ Tue, 30 Jun 2020 01:29:33: 2000000 INFO @ Tue, 30 Jun 2020 01:29:37: 5000000 INFO @ Tue, 30 Jun 2020 01:29:42: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:29:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX645133/SRX645133.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX645133/SRX645133.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX645133/SRX645133.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX645133/SRX645133.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:29:45: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:29:45: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:29:48: 6000000 INFO @ Tue, 30 Jun 2020 01:29:52: 4000000 INFO @ Tue, 30 Jun 2020 01:29:55: 1000000 INFO @ Tue, 30 Jun 2020 01:29:59: 7000000 INFO @ Tue, 30 Jun 2020 01:30:02: 5000000 INFO @ Tue, 30 Jun 2020 01:30:05: 2000000 INFO @ Tue, 30 Jun 2020 01:30:09: 8000000 INFO @ Tue, 30 Jun 2020 01:30:12: 6000000 INFO @ Tue, 30 Jun 2020 01:30:15: 3000000 INFO @ Tue, 30 Jun 2020 01:30:20: 9000000 INFO @ Tue, 30 Jun 2020 01:30:22: 7000000 INFO @ Tue, 30 Jun 2020 01:30:25: 4000000 INFO @ Tue, 30 Jun 2020 01:30:30: 10000000 INFO @ Tue, 30 Jun 2020 01:30:32: 8000000 INFO @ Tue, 30 Jun 2020 01:30:35: 5000000 INFO @ Tue, 30 Jun 2020 01:30:40: 11000000 INFO @ Tue, 30 Jun 2020 01:30:42: 9000000 INFO @ Tue, 30 Jun 2020 01:30:45: 6000000 INFO @ Tue, 30 Jun 2020 01:30:50: 12000000 INFO @ Tue, 30 Jun 2020 01:30:51: 10000000 INFO @ Tue, 30 Jun 2020 01:30:54: 7000000 INFO @ Tue, 30 Jun 2020 01:31:01: 13000000 INFO @ Tue, 30 Jun 2020 01:31:01: 11000000 INFO @ Tue, 30 Jun 2020 01:31:04: 8000000 INFO @ Tue, 30 Jun 2020 01:31:11: 14000000 INFO @ Tue, 30 Jun 2020 01:31:11: 12000000 INFO @ Tue, 30 Jun 2020 01:31:14: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 01:31:21: 15000000 INFO @ Tue, 30 Jun 2020 01:31:21: 13000000 INFO @ Tue, 30 Jun 2020 01:31:24: 10000000 INFO @ Tue, 30 Jun 2020 01:31:28: #1 tag size is determined as 100 bps INFO @ Tue, 30 Jun 2020 01:31:28: #1 tag size = 100 INFO @ Tue, 30 Jun 2020 01:31:28: #1 total tags in treatment: 15751304 INFO @ Tue, 30 Jun 2020 01:31:28: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:31:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:31:29: #1 tags after filtering in treatment: 15751304 INFO @ Tue, 30 Jun 2020 01:31:29: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:31:29: #1 finished! INFO @ Tue, 30 Jun 2020 01:31:29: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:31:29: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:31:30: #2 number of paired peaks: 56 WARNING @ Tue, 30 Jun 2020 01:31:30: Too few paired peaks (56) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:31:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX645133/SRX645133.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX645133/SRX645133.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX645133/SRX645133.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX645133/SRX645133.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 01:31:30: 14000000 INFO @ Tue, 30 Jun 2020 01:31:33: 11000000 INFO @ Tue, 30 Jun 2020 01:31:39: 15000000 INFO @ Tue, 30 Jun 2020 01:31:42: 12000000 INFO @ Tue, 30 Jun 2020 01:31:46: #1 tag size is determined as 100 bps INFO @ Tue, 30 Jun 2020 01:31:46: #1 tag size = 100 INFO @ Tue, 30 Jun 2020 01:31:46: #1 total tags in treatment: 15751304 INFO @ Tue, 30 Jun 2020 01:31:46: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:31:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:31:46: #1 tags after filtering in treatment: 15751304 INFO @ Tue, 30 Jun 2020 01:31:46: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:31:46: #1 finished! INFO @ Tue, 30 Jun 2020 01:31:46: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:31:46: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:31:47: #2 number of paired peaks: 56 WARNING @ Tue, 30 Jun 2020 01:31:47: Too few paired peaks (56) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:31:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX645133/SRX645133.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX645133/SRX645133.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX645133/SRX645133.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX645133/SRX645133.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 01:31:50: 13000000 BigWig に変換しました。 INFO @ Tue, 30 Jun 2020 01:31:58: 14000000 INFO @ Tue, 30 Jun 2020 01:32:06: 15000000 INFO @ Tue, 30 Jun 2020 01:32:12: #1 tag size is determined as 100 bps INFO @ Tue, 30 Jun 2020 01:32:12: #1 tag size = 100 INFO @ Tue, 30 Jun 2020 01:32:12: #1 total tags in treatment: 15751304 INFO @ Tue, 30 Jun 2020 01:32:12: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:32:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:32:12: #1 tags after filtering in treatment: 15751304 INFO @ Tue, 30 Jun 2020 01:32:12: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:32:12: #1 finished! INFO @ Tue, 30 Jun 2020 01:32:12: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:32:12: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:32:13: #2 number of paired peaks: 56 WARNING @ Tue, 30 Jun 2020 01:32:13: Too few paired peaks (56) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:32:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX645133/SRX645133.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX645133/SRX645133.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX645133/SRX645133.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX645133/SRX645133.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling