Job ID = 6528435 SRX = SRX645106 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T15:00:58 prefetch.2.10.7: 1) Downloading 'SRR1505705'... 2020-06-29T15:00:58 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:09:17 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:09:17 prefetch.2.10.7: 1) 'SRR1505705' was downloaded successfully Read 29883592 spots for SRR1505705/SRR1505705.sra Written 29883592 spots for SRR1505705/SRR1505705.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:00 29883592 reads; of these: 29883592 (100.00%) were unpaired; of these: 17317061 (57.95%) aligned 0 times 10211269 (34.17%) aligned exactly 1 time 2355262 (7.88%) aligned >1 times 42.05% overall alignment rate Time searching: 00:11:00 Overall time: 00:11:00 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 738911 / 12566531 = 0.0588 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:47:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX645106/SRX645106.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX645106/SRX645106.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX645106/SRX645106.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX645106/SRX645106.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:47:54: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:47:54: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:48:03: 1000000 INFO @ Tue, 30 Jun 2020 00:48:13: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:48:23: 3000000 INFO @ Tue, 30 Jun 2020 00:48:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX645106/SRX645106.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX645106/SRX645106.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX645106/SRX645106.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX645106/SRX645106.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:48:24: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:48:24: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:48:33: 4000000 INFO @ Tue, 30 Jun 2020 00:48:34: 1000000 INFO @ Tue, 30 Jun 2020 00:48:44: 5000000 INFO @ Tue, 30 Jun 2020 00:48:44: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:48:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX645106/SRX645106.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX645106/SRX645106.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX645106/SRX645106.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX645106/SRX645106.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:48:54: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:48:54: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:48:54: 3000000 INFO @ Tue, 30 Jun 2020 00:48:54: 6000000 INFO @ Tue, 30 Jun 2020 00:49:03: 1000000 INFO @ Tue, 30 Jun 2020 00:49:05: 4000000 INFO @ Tue, 30 Jun 2020 00:49:05: 7000000 INFO @ Tue, 30 Jun 2020 00:49:13: 2000000 INFO @ Tue, 30 Jun 2020 00:49:15: 5000000 INFO @ Tue, 30 Jun 2020 00:49:15: 8000000 INFO @ Tue, 30 Jun 2020 00:49:22: 3000000 INFO @ Tue, 30 Jun 2020 00:49:26: 6000000 INFO @ Tue, 30 Jun 2020 00:49:26: 9000000 INFO @ Tue, 30 Jun 2020 00:49:32: 4000000 INFO @ Tue, 30 Jun 2020 00:49:36: 7000000 INFO @ Tue, 30 Jun 2020 00:49:37: 10000000 INFO @ Tue, 30 Jun 2020 00:49:41: 5000000 INFO @ Tue, 30 Jun 2020 00:49:46: 8000000 INFO @ Tue, 30 Jun 2020 00:49:47: 11000000 INFO @ Tue, 30 Jun 2020 00:49:51: 6000000 INFO @ Tue, 30 Jun 2020 00:49:56: 9000000 INFO @ Tue, 30 Jun 2020 00:49:56: #1 tag size is determined as 100 bps INFO @ Tue, 30 Jun 2020 00:49:56: #1 tag size = 100 INFO @ Tue, 30 Jun 2020 00:49:56: #1 total tags in treatment: 11827620 INFO @ Tue, 30 Jun 2020 00:49:56: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:49:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:49:56: #1 tags after filtering in treatment: 11827620 INFO @ Tue, 30 Jun 2020 00:49:56: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:49:56: #1 finished! INFO @ Tue, 30 Jun 2020 00:49:56: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:49:56: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:49:57: #2 number of paired peaks: 3 WARNING @ Tue, 30 Jun 2020 00:49:57: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:49:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX645106/SRX645106.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX645106/SRX645106.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX645106/SRX645106.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX645106/SRX645106.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:50:00: 7000000 INFO @ Tue, 30 Jun 2020 00:50:06: 10000000 INFO @ Tue, 30 Jun 2020 00:50:09: 8000000 INFO @ Tue, 30 Jun 2020 00:50:15: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:50:19: 9000000 INFO @ Tue, 30 Jun 2020 00:50:23: #1 tag size is determined as 100 bps INFO @ Tue, 30 Jun 2020 00:50:23: #1 tag size = 100 INFO @ Tue, 30 Jun 2020 00:50:23: #1 total tags in treatment: 11827620 INFO @ Tue, 30 Jun 2020 00:50:23: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:50:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:50:24: #1 tags after filtering in treatment: 11827620 INFO @ Tue, 30 Jun 2020 00:50:24: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:50:24: #1 finished! INFO @ Tue, 30 Jun 2020 00:50:24: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:50:24: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:50:24: #2 number of paired peaks: 3 WARNING @ Tue, 30 Jun 2020 00:50:24: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:50:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX645106/SRX645106.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX645106/SRX645106.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX645106/SRX645106.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX645106/SRX645106.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:50:29: 10000000 INFO @ Tue, 30 Jun 2020 00:50:38: 11000000 INFO @ Tue, 30 Jun 2020 00:50:46: #1 tag size is determined as 100 bps INFO @ Tue, 30 Jun 2020 00:50:46: #1 tag size = 100 INFO @ Tue, 30 Jun 2020 00:50:46: #1 total tags in treatment: 11827620 INFO @ Tue, 30 Jun 2020 00:50:46: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:50:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:50:46: #1 tags after filtering in treatment: 11827620 INFO @ Tue, 30 Jun 2020 00:50:46: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:50:46: #1 finished! INFO @ Tue, 30 Jun 2020 00:50:46: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:50:46: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:50:47: #2 number of paired peaks: 3 WARNING @ Tue, 30 Jun 2020 00:50:47: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:50:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX645106/SRX645106.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX645106/SRX645106.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX645106/SRX645106.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX645106/SRX645106.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。