Job ID = 6528431 SRX = SRX645100 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T15:25:32 prefetch.2.10.7: 1) Downloading 'SRR1505699'... 2020-06-29T15:25:32 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:32:29 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:32:29 prefetch.2.10.7: 1) 'SRR1505699' was downloaded successfully Read 25534902 spots for SRR1505699/SRR1505699.sra Written 25534902 spots for SRR1505699/SRR1505699.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:29 25534902 reads; of these: 25534902 (100.00%) were unpaired; of these: 13244262 (51.87%) aligned 0 times 8550301 (33.48%) aligned exactly 1 time 3740339 (14.65%) aligned >1 times 48.13% overall alignment rate Time searching: 00:12:29 Overall time: 00:12:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2364093 / 12290640 = 0.1923 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:08:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX645100/SRX645100.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX645100/SRX645100.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX645100/SRX645100.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX645100/SRX645100.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:08:52: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:08:52: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:09:00: 1000000 INFO @ Tue, 30 Jun 2020 01:09:08: 2000000 INFO @ Tue, 30 Jun 2020 01:09:17: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:09:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX645100/SRX645100.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX645100/SRX645100.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX645100/SRX645100.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX645100/SRX645100.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:09:22: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:09:22: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:09:25: 4000000 INFO @ Tue, 30 Jun 2020 01:09:30: 1000000 INFO @ Tue, 30 Jun 2020 01:09:34: 5000000 INFO @ Tue, 30 Jun 2020 01:09:39: 2000000 INFO @ Tue, 30 Jun 2020 01:09:43: 6000000 INFO @ Tue, 30 Jun 2020 01:09:47: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:09:51: 7000000 INFO @ Tue, 30 Jun 2020 01:09:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX645100/SRX645100.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX645100/SRX645100.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX645100/SRX645100.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX645100/SRX645100.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:09:52: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:09:52: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:09:56: 4000000 INFO @ Tue, 30 Jun 2020 01:10:00: 8000000 INFO @ Tue, 30 Jun 2020 01:10:01: 1000000 INFO @ Tue, 30 Jun 2020 01:10:05: 5000000 INFO @ Tue, 30 Jun 2020 01:10:09: 9000000 INFO @ Tue, 30 Jun 2020 01:10:10: 2000000 INFO @ Tue, 30 Jun 2020 01:10:14: 6000000 INFO @ Tue, 30 Jun 2020 01:10:18: #1 tag size is determined as 100 bps INFO @ Tue, 30 Jun 2020 01:10:18: #1 tag size = 100 INFO @ Tue, 30 Jun 2020 01:10:18: #1 total tags in treatment: 9926547 INFO @ Tue, 30 Jun 2020 01:10:18: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:10:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:10:18: #1 tags after filtering in treatment: 9926547 INFO @ Tue, 30 Jun 2020 01:10:18: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:10:18: #1 finished! INFO @ Tue, 30 Jun 2020 01:10:18: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:10:18: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:10:19: 3000000 INFO @ Tue, 30 Jun 2020 01:10:19: #2 number of paired peaks: 91 WARNING @ Tue, 30 Jun 2020 01:10:19: Too few paired peaks (91) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:10:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX645100/SRX645100.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX645100/SRX645100.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX645100/SRX645100.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX645100/SRX645100.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 01:10:22: 7000000 INFO @ Tue, 30 Jun 2020 01:10:27: 4000000 INFO @ Tue, 30 Jun 2020 01:10:31: 8000000 INFO @ Tue, 30 Jun 2020 01:10:36: 5000000 INFO @ Tue, 30 Jun 2020 01:10:39: 9000000 INFO @ Tue, 30 Jun 2020 01:10:45: 6000000 INFO @ Tue, 30 Jun 2020 01:10:47: #1 tag size is determined as 100 bps INFO @ Tue, 30 Jun 2020 01:10:47: #1 tag size = 100 INFO @ Tue, 30 Jun 2020 01:10:47: #1 total tags in treatment: 9926547 INFO @ Tue, 30 Jun 2020 01:10:47: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:10:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:10:48: #1 tags after filtering in treatment: 9926547 INFO @ Tue, 30 Jun 2020 01:10:48: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:10:48: #1 finished! INFO @ Tue, 30 Jun 2020 01:10:48: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:10:48: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:10:48: #2 number of paired peaks: 91 WARNING @ Tue, 30 Jun 2020 01:10:48: Too few paired peaks (91) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:10:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX645100/SRX645100.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX645100/SRX645100.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX645100/SRX645100.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX645100/SRX645100.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 01:10:53: 7000000 INFO @ Tue, 30 Jun 2020 01:11:00: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 01:11:08: 9000000 INFO @ Tue, 30 Jun 2020 01:11:15: #1 tag size is determined as 100 bps INFO @ Tue, 30 Jun 2020 01:11:15: #1 tag size = 100 INFO @ Tue, 30 Jun 2020 01:11:15: #1 total tags in treatment: 9926547 INFO @ Tue, 30 Jun 2020 01:11:15: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:11:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:11:16: #1 tags after filtering in treatment: 9926547 INFO @ Tue, 30 Jun 2020 01:11:16: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:11:16: #1 finished! INFO @ Tue, 30 Jun 2020 01:11:16: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:11:16: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:11:16: #2 number of paired peaks: 91 WARNING @ Tue, 30 Jun 2020 01:11:16: Too few paired peaks (91) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:11:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX645100/SRX645100.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX645100/SRX645100.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX645100/SRX645100.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX645100/SRX645100.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。