Job ID = 12265366 SRX = SRX6430379 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 30683773 spots for SRR9669862/SRR9669862.sra Written 30683773 spots for SRR9669862/SRR9669862.sra fastq に変換しました。 bowtie でマッピング中... Your job 12265658 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:48:13 30683773 reads; of these: 30683773 (100.00%) were paired; of these: 9260002 (30.18%) aligned concordantly 0 times 16948972 (55.24%) aligned concordantly exactly 1 time 4474799 (14.58%) aligned concordantly >1 times ---- 9260002 pairs aligned concordantly 0 times; of these: 3072953 (33.19%) aligned discordantly 1 time ---- 6187049 pairs aligned 0 times concordantly or discordantly; of these: 12374098 mates make up the pairs; of these: 10365117 (83.76%) aligned 0 times 839634 (6.79%) aligned exactly 1 time 1169347 (9.45%) aligned >1 times 83.11% overall alignment rate Time searching: 00:48:13 Overall time: 00:48:13 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 3067444 / 24117872 = 0.1272 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:46:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6430379/SRX6430379.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6430379/SRX6430379.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6430379/SRX6430379.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6430379/SRX6430379.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:46:52: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:46:52: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:46:59: 1000000 INFO @ Sat, 03 Apr 2021 07:47:05: 2000000 INFO @ Sat, 03 Apr 2021 07:47:11: 3000000 INFO @ Sat, 03 Apr 2021 07:47:17: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:47:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6430379/SRX6430379.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6430379/SRX6430379.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6430379/SRX6430379.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6430379/SRX6430379.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:47:22: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:47:22: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:47:23: 5000000 INFO @ Sat, 03 Apr 2021 07:47:30: 1000000 INFO @ Sat, 03 Apr 2021 07:47:30: 6000000 INFO @ Sat, 03 Apr 2021 07:47:37: 7000000 INFO @ Sat, 03 Apr 2021 07:47:38: 2000000 INFO @ Sat, 03 Apr 2021 07:47:45: 8000000 INFO @ Sat, 03 Apr 2021 07:47:46: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:47:52: 9000000 INFO @ Sat, 03 Apr 2021 07:47:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6430379/SRX6430379.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6430379/SRX6430379.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6430379/SRX6430379.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6430379/SRX6430379.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:47:52: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:47:52: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:47:54: 4000000 INFO @ Sat, 03 Apr 2021 07:47:59: 10000000 INFO @ Sat, 03 Apr 2021 07:47:59: 1000000 INFO @ Sat, 03 Apr 2021 07:48:02: 5000000 INFO @ Sat, 03 Apr 2021 07:48:06: 11000000 INFO @ Sat, 03 Apr 2021 07:48:07: 2000000 INFO @ Sat, 03 Apr 2021 07:48:10: 6000000 INFO @ Sat, 03 Apr 2021 07:48:14: 12000000 INFO @ Sat, 03 Apr 2021 07:48:14: 3000000 INFO @ Sat, 03 Apr 2021 07:48:18: 7000000 INFO @ Sat, 03 Apr 2021 07:48:21: 13000000 INFO @ Sat, 03 Apr 2021 07:48:21: 4000000 INFO @ Sat, 03 Apr 2021 07:48:26: 8000000 INFO @ Sat, 03 Apr 2021 07:48:28: 14000000 INFO @ Sat, 03 Apr 2021 07:48:29: 5000000 INFO @ Sat, 03 Apr 2021 07:48:34: 9000000 INFO @ Sat, 03 Apr 2021 07:48:35: 15000000 INFO @ Sat, 03 Apr 2021 07:48:36: 6000000 INFO @ Sat, 03 Apr 2021 07:48:42: 10000000 INFO @ Sat, 03 Apr 2021 07:48:43: 16000000 INFO @ Sat, 03 Apr 2021 07:48:43: 7000000 INFO @ Sat, 03 Apr 2021 07:48:50: 17000000 INFO @ Sat, 03 Apr 2021 07:48:50: 8000000 INFO @ Sat, 03 Apr 2021 07:48:51: 11000000 INFO @ Sat, 03 Apr 2021 07:48:57: 18000000 INFO @ Sat, 03 Apr 2021 07:48:58: 9000000 INFO @ Sat, 03 Apr 2021 07:48:59: 12000000 INFO @ Sat, 03 Apr 2021 07:49:05: 19000000 INFO @ Sat, 03 Apr 2021 07:49:05: 10000000 INFO @ Sat, 03 Apr 2021 07:49:07: 13000000 INFO @ Sat, 03 Apr 2021 07:49:12: 20000000 INFO @ Sat, 03 Apr 2021 07:49:12: 11000000 INFO @ Sat, 03 Apr 2021 07:49:16: 14000000 INFO @ Sat, 03 Apr 2021 07:49:19: 21000000 INFO @ Sat, 03 Apr 2021 07:49:20: 12000000 INFO @ Sat, 03 Apr 2021 07:49:24: 15000000 INFO @ Sat, 03 Apr 2021 07:49:27: 22000000 INFO @ Sat, 03 Apr 2021 07:49:27: 13000000 INFO @ Sat, 03 Apr 2021 07:49:33: 16000000 INFO @ Sat, 03 Apr 2021 07:49:34: 23000000 INFO @ Sat, 03 Apr 2021 07:49:34: 14000000 INFO @ Sat, 03 Apr 2021 07:49:41: 24000000 INFO @ Sat, 03 Apr 2021 07:49:42: 15000000 INFO @ Sat, 03 Apr 2021 07:49:42: 17000000 INFO @ Sat, 03 Apr 2021 07:49:49: 25000000 INFO @ Sat, 03 Apr 2021 07:49:49: 16000000 INFO @ Sat, 03 Apr 2021 07:49:51: 18000000 INFO @ Sat, 03 Apr 2021 07:49:56: 26000000 INFO @ Sat, 03 Apr 2021 07:49:57: 17000000 INFO @ Sat, 03 Apr 2021 07:49:59: 19000000 INFO @ Sat, 03 Apr 2021 07:50:03: 27000000 INFO @ Sat, 03 Apr 2021 07:50:04: 18000000 INFO @ Sat, 03 Apr 2021 07:50:08: 20000000 INFO @ Sat, 03 Apr 2021 07:50:10: 28000000 INFO @ Sat, 03 Apr 2021 07:50:11: 19000000 INFO @ Sat, 03 Apr 2021 07:50:17: 21000000 INFO @ Sat, 03 Apr 2021 07:50:18: 20000000 INFO @ Sat, 03 Apr 2021 07:50:18: 29000000 INFO @ Sat, 03 Apr 2021 07:50:25: 21000000 INFO @ Sat, 03 Apr 2021 07:50:25: 22000000 INFO @ Sat, 03 Apr 2021 07:50:25: 30000000 INFO @ Sat, 03 Apr 2021 07:50:32: 22000000 INFO @ Sat, 03 Apr 2021 07:50:33: 31000000 INFO @ Sat, 03 Apr 2021 07:50:34: 23000000 INFO @ Sat, 03 Apr 2021 07:50:39: 23000000 INFO @ Sat, 03 Apr 2021 07:50:40: 32000000 INFO @ Sat, 03 Apr 2021 07:50:42: 24000000 INFO @ Sat, 03 Apr 2021 07:50:47: 24000000 INFO @ Sat, 03 Apr 2021 07:50:47: 33000000 INFO @ Sat, 03 Apr 2021 07:50:51: 25000000 INFO @ Sat, 03 Apr 2021 07:50:54: 25000000 INFO @ Sat, 03 Apr 2021 07:50:54: 34000000 INFO @ Sat, 03 Apr 2021 07:51:00: 26000000 INFO @ Sat, 03 Apr 2021 07:51:01: 26000000 INFO @ Sat, 03 Apr 2021 07:51:02: 35000000 INFO @ Sat, 03 Apr 2021 07:51:09: 27000000 INFO @ Sat, 03 Apr 2021 07:51:09: 36000000 INFO @ Sat, 03 Apr 2021 07:51:09: 27000000 INFO @ Sat, 03 Apr 2021 07:51:16: 37000000 INFO @ Sat, 03 Apr 2021 07:51:17: 28000000 INFO @ Sat, 03 Apr 2021 07:51:18: 28000000 INFO @ Sat, 03 Apr 2021 07:51:23: 38000000 INFO @ Sat, 03 Apr 2021 07:51:27: 29000000 INFO @ Sat, 03 Apr 2021 07:51:27: 29000000 INFO @ Sat, 03 Apr 2021 07:51:30: 39000000 INFO @ Sat, 03 Apr 2021 07:51:35: 30000000 INFO @ Sat, 03 Apr 2021 07:51:36: 30000000 INFO @ Sat, 03 Apr 2021 07:51:37: 40000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 07:51:44: 31000000 INFO @ Sat, 03 Apr 2021 07:51:44: 41000000 INFO @ Sat, 03 Apr 2021 07:51:45: 31000000 INFO @ Sat, 03 Apr 2021 07:51:51: 42000000 INFO @ Sat, 03 Apr 2021 07:51:52: 32000000 INFO @ Sat, 03 Apr 2021 07:51:54: 32000000 INFO @ Sat, 03 Apr 2021 07:51:58: 43000000 INFO @ Sat, 03 Apr 2021 07:52:00: 33000000 INFO @ Sat, 03 Apr 2021 07:52:02: 33000000 INFO @ Sat, 03 Apr 2021 07:52:05: 44000000 INFO @ Sat, 03 Apr 2021 07:52:09: 34000000 INFO @ Sat, 03 Apr 2021 07:52:10: 34000000 INFO @ Sat, 03 Apr 2021 07:52:11: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:52:11: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:52:11: #1 total tags in treatment: 18812606 INFO @ Sat, 03 Apr 2021 07:52:11: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:52:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:52:11: #1 tags after filtering in treatment: 14986140 INFO @ Sat, 03 Apr 2021 07:52:11: #1 Redundant rate of treatment: 0.20 INFO @ Sat, 03 Apr 2021 07:52:11: #1 finished! INFO @ Sat, 03 Apr 2021 07:52:11: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:52:11: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:52:12: #2 number of paired peaks: 79 WARNING @ Sat, 03 Apr 2021 07:52:12: Too few paired peaks (79) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 07:52:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6430379/SRX6430379.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 26 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430379/SRX6430379.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430379/SRX6430379.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430379/SRX6430379.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:52:17: 35000000 INFO @ Sat, 03 Apr 2021 07:52:19: 35000000 INFO @ Sat, 03 Apr 2021 07:52:24: 36000000 INFO @ Sat, 03 Apr 2021 07:52:27: 36000000 INFO @ Sat, 03 Apr 2021 07:52:31: 37000000 INFO @ Sat, 03 Apr 2021 07:52:36: 37000000 INFO @ Sat, 03 Apr 2021 07:52:38: 38000000 INFO @ Sat, 03 Apr 2021 07:52:44: 38000000 INFO @ Sat, 03 Apr 2021 07:52:44: 39000000 INFO @ Sat, 03 Apr 2021 07:52:51: 40000000 INFO @ Sat, 03 Apr 2021 07:52:51: 39000000 INFO @ Sat, 03 Apr 2021 07:52:58: 41000000 INFO @ Sat, 03 Apr 2021 07:52:59: 40000000 INFO @ Sat, 03 Apr 2021 07:53:04: 42000000 BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 07:53:07: 41000000 INFO @ Sat, 03 Apr 2021 07:53:11: 43000000 INFO @ Sat, 03 Apr 2021 07:53:15: 42000000 INFO @ Sat, 03 Apr 2021 07:53:18: 44000000 INFO @ Sat, 03 Apr 2021 07:53:22: 43000000 INFO @ Sat, 03 Apr 2021 07:53:23: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:53:23: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:53:23: #1 total tags in treatment: 18812606 INFO @ Sat, 03 Apr 2021 07:53:23: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:53:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:53:24: #1 tags after filtering in treatment: 14986140 INFO @ Sat, 03 Apr 2021 07:53:24: #1 Redundant rate of treatment: 0.20 INFO @ Sat, 03 Apr 2021 07:53:24: #1 finished! INFO @ Sat, 03 Apr 2021 07:53:24: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:53:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:53:25: #2 number of paired peaks: 79 WARNING @ Sat, 03 Apr 2021 07:53:25: Too few paired peaks (79) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 07:53:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6430379/SRX6430379.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 19 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430379/SRX6430379.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430379/SRX6430379.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430379/SRX6430379.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:53:29: 44000000 INFO @ Sat, 03 Apr 2021 07:53:36: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:53:36: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:53:36: #1 total tags in treatment: 18812606 INFO @ Sat, 03 Apr 2021 07:53:36: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:53:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:53:36: #1 tags after filtering in treatment: 14986140 INFO @ Sat, 03 Apr 2021 07:53:36: #1 Redundant rate of treatment: 0.20 INFO @ Sat, 03 Apr 2021 07:53:36: #1 finished! INFO @ Sat, 03 Apr 2021 07:53:36: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:53:36: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:53:37: #2 number of paired peaks: 79 WARNING @ Sat, 03 Apr 2021 07:53:37: Too few paired peaks (79) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 07:53:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6430379/SRX6430379.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430379/SRX6430379.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430379/SRX6430379.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430379/SRX6430379.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling