Job ID = 12265364 SRX = SRX6430377 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 30932397 spots for SRR9669864/SRR9669864.sra Written 30932397 spots for SRR9669864/SRR9669864.sra fastq に変換しました。 bowtie でマッピング中... Your job 12265673 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:51:21 30932397 reads; of these: 30932397 (100.00%) were paired; of these: 8311797 (26.87%) aligned concordantly 0 times 17472851 (56.49%) aligned concordantly exactly 1 time 5147749 (16.64%) aligned concordantly >1 times ---- 8311797 pairs aligned concordantly 0 times; of these: 2901451 (34.91%) aligned discordantly 1 time ---- 5410346 pairs aligned 0 times concordantly or discordantly; of these: 10820692 mates make up the pairs; of these: 8837994 (81.68%) aligned 0 times 749385 (6.93%) aligned exactly 1 time 1233313 (11.40%) aligned >1 times 85.71% overall alignment rate Time searching: 00:51:21 Overall time: 00:51:21 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 2116974 / 25164854 = 0.0841 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:50:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6430377/SRX6430377.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6430377/SRX6430377.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6430377/SRX6430377.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6430377/SRX6430377.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:50:38: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:50:38: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:50:44: 1000000 INFO @ Sat, 03 Apr 2021 07:50:49: 2000000 INFO @ Sat, 03 Apr 2021 07:50:55: 3000000 INFO @ Sat, 03 Apr 2021 07:51:00: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:51:06: 5000000 INFO @ Sat, 03 Apr 2021 07:51:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6430377/SRX6430377.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6430377/SRX6430377.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6430377/SRX6430377.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6430377/SRX6430377.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:51:08: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:51:08: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:51:11: 6000000 INFO @ Sat, 03 Apr 2021 07:51:14: 1000000 INFO @ Sat, 03 Apr 2021 07:51:17: 7000000 INFO @ Sat, 03 Apr 2021 07:51:20: 2000000 INFO @ Sat, 03 Apr 2021 07:51:23: 8000000 INFO @ Sat, 03 Apr 2021 07:51:26: 3000000 INFO @ Sat, 03 Apr 2021 07:51:29: 9000000 INFO @ Sat, 03 Apr 2021 07:51:31: 4000000 INFO @ Sat, 03 Apr 2021 07:51:35: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:51:37: 5000000 INFO @ Sat, 03 Apr 2021 07:51:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6430377/SRX6430377.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6430377/SRX6430377.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6430377/SRX6430377.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6430377/SRX6430377.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:51:38: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:51:38: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:51:41: 11000000 INFO @ Sat, 03 Apr 2021 07:51:43: 6000000 INFO @ Sat, 03 Apr 2021 07:51:44: 1000000 INFO @ Sat, 03 Apr 2021 07:51:47: 12000000 INFO @ Sat, 03 Apr 2021 07:51:49: 7000000 INFO @ Sat, 03 Apr 2021 07:51:50: 2000000 INFO @ Sat, 03 Apr 2021 07:51:53: 13000000 INFO @ Sat, 03 Apr 2021 07:51:55: 8000000 INFO @ Sat, 03 Apr 2021 07:51:56: 3000000 INFO @ Sat, 03 Apr 2021 07:51:59: 14000000 INFO @ Sat, 03 Apr 2021 07:52:01: 9000000 INFO @ Sat, 03 Apr 2021 07:52:02: 4000000 INFO @ Sat, 03 Apr 2021 07:52:04: 15000000 INFO @ Sat, 03 Apr 2021 07:52:07: 10000000 INFO @ Sat, 03 Apr 2021 07:52:08: 5000000 INFO @ Sat, 03 Apr 2021 07:52:10: 16000000 INFO @ Sat, 03 Apr 2021 07:52:13: 11000000 INFO @ Sat, 03 Apr 2021 07:52:14: 6000000 INFO @ Sat, 03 Apr 2021 07:52:16: 17000000 INFO @ Sat, 03 Apr 2021 07:52:18: 12000000 INFO @ Sat, 03 Apr 2021 07:52:20: 7000000 INFO @ Sat, 03 Apr 2021 07:52:22: 18000000 INFO @ Sat, 03 Apr 2021 07:52:24: 13000000 INFO @ Sat, 03 Apr 2021 07:52:26: 8000000 INFO @ Sat, 03 Apr 2021 07:52:28: 19000000 INFO @ Sat, 03 Apr 2021 07:52:30: 14000000 INFO @ Sat, 03 Apr 2021 07:52:32: 9000000 INFO @ Sat, 03 Apr 2021 07:52:34: 20000000 INFO @ Sat, 03 Apr 2021 07:52:35: 15000000 INFO @ Sat, 03 Apr 2021 07:52:38: 10000000 INFO @ Sat, 03 Apr 2021 07:52:39: 21000000 INFO @ Sat, 03 Apr 2021 07:52:41: 16000000 INFO @ Sat, 03 Apr 2021 07:52:43: 11000000 INFO @ Sat, 03 Apr 2021 07:52:45: 22000000 INFO @ Sat, 03 Apr 2021 07:52:47: 17000000 INFO @ Sat, 03 Apr 2021 07:52:49: 12000000 INFO @ Sat, 03 Apr 2021 07:52:51: 23000000 INFO @ Sat, 03 Apr 2021 07:52:52: 18000000 INFO @ Sat, 03 Apr 2021 07:52:55: 13000000 INFO @ Sat, 03 Apr 2021 07:52:56: 24000000 INFO @ Sat, 03 Apr 2021 07:52:58: 19000000 INFO @ Sat, 03 Apr 2021 07:53:01: 14000000 INFO @ Sat, 03 Apr 2021 07:53:02: 25000000 INFO @ Sat, 03 Apr 2021 07:53:04: 20000000 INFO @ Sat, 03 Apr 2021 07:53:07: 15000000 INFO @ Sat, 03 Apr 2021 07:53:08: 26000000 INFO @ Sat, 03 Apr 2021 07:53:09: 21000000 INFO @ Sat, 03 Apr 2021 07:53:13: 16000000 INFO @ Sat, 03 Apr 2021 07:53:13: 27000000 INFO @ Sat, 03 Apr 2021 07:53:15: 22000000 INFO @ Sat, 03 Apr 2021 07:53:19: 17000000 INFO @ Sat, 03 Apr 2021 07:53:19: 28000000 INFO @ Sat, 03 Apr 2021 07:53:20: 23000000 INFO @ Sat, 03 Apr 2021 07:53:25: 18000000 INFO @ Sat, 03 Apr 2021 07:53:25: 29000000 INFO @ Sat, 03 Apr 2021 07:53:26: 24000000 INFO @ Sat, 03 Apr 2021 07:53:30: 19000000 INFO @ Sat, 03 Apr 2021 07:53:31: 30000000 INFO @ Sat, 03 Apr 2021 07:53:32: 25000000 INFO @ Sat, 03 Apr 2021 07:53:36: 20000000 INFO @ Sat, 03 Apr 2021 07:53:36: 31000000 INFO @ Sat, 03 Apr 2021 07:53:37: 26000000 INFO @ Sat, 03 Apr 2021 07:53:42: 21000000 INFO @ Sat, 03 Apr 2021 07:53:42: 32000000 INFO @ Sat, 03 Apr 2021 07:53:43: 27000000 INFO @ Sat, 03 Apr 2021 07:53:48: 22000000 INFO @ Sat, 03 Apr 2021 07:53:48: 33000000 INFO @ Sat, 03 Apr 2021 07:53:48: 28000000 INFO @ Sat, 03 Apr 2021 07:53:53: 23000000 INFO @ Sat, 03 Apr 2021 07:53:53: 34000000 INFO @ Sat, 03 Apr 2021 07:53:54: 29000000 INFO @ Sat, 03 Apr 2021 07:53:59: 24000000 INFO @ Sat, 03 Apr 2021 07:53:59: 35000000 INFO @ Sat, 03 Apr 2021 07:54:00: 30000000 INFO @ Sat, 03 Apr 2021 07:54:04: 25000000 INFO @ Sat, 03 Apr 2021 07:54:04: 36000000 INFO @ Sat, 03 Apr 2021 07:54:05: 31000000 INFO @ Sat, 03 Apr 2021 07:54:10: 26000000 INFO @ Sat, 03 Apr 2021 07:54:10: 37000000 INFO @ Sat, 03 Apr 2021 07:54:11: 32000000 INFO @ Sat, 03 Apr 2021 07:54:15: 27000000 INFO @ Sat, 03 Apr 2021 07:54:16: 38000000 INFO @ Sat, 03 Apr 2021 07:54:16: 33000000 INFO @ Sat, 03 Apr 2021 07:54:21: 28000000 INFO @ Sat, 03 Apr 2021 07:54:21: 39000000 INFO @ Sat, 03 Apr 2021 07:54:22: 34000000 INFO @ Sat, 03 Apr 2021 07:54:26: 29000000 INFO @ Sat, 03 Apr 2021 07:54:27: 40000000 INFO @ Sat, 03 Apr 2021 07:54:27: 35000000 INFO @ Sat, 03 Apr 2021 07:54:32: 30000000 INFO @ Sat, 03 Apr 2021 07:54:32: 41000000 INFO @ Sat, 03 Apr 2021 07:54:33: 36000000 INFO @ Sat, 03 Apr 2021 07:54:37: 31000000 INFO @ Sat, 03 Apr 2021 07:54:38: 42000000 INFO @ Sat, 03 Apr 2021 07:54:38: 37000000 INFO @ Sat, 03 Apr 2021 07:54:43: 32000000 INFO @ Sat, 03 Apr 2021 07:54:43: 43000000 INFO @ Sat, 03 Apr 2021 07:54:44: 38000000 INFO @ Sat, 03 Apr 2021 07:54:48: 33000000 INFO @ Sat, 03 Apr 2021 07:54:49: 44000000 INFO @ Sat, 03 Apr 2021 07:54:49: 39000000 INFO @ Sat, 03 Apr 2021 07:54:54: 34000000 INFO @ Sat, 03 Apr 2021 07:54:54: 45000000 INFO @ Sat, 03 Apr 2021 07:54:55: 40000000 INFO @ Sat, 03 Apr 2021 07:54:59: 35000000 INFO @ Sat, 03 Apr 2021 07:55:00: 46000000 INFO @ Sat, 03 Apr 2021 07:55:00: 41000000 INFO @ Sat, 03 Apr 2021 07:55:04: 36000000 INFO @ Sat, 03 Apr 2021 07:55:05: 47000000 INFO @ Sat, 03 Apr 2021 07:55:06: 42000000 INFO @ Sat, 03 Apr 2021 07:55:10: 37000000 INFO @ Sat, 03 Apr 2021 07:55:11: 48000000 INFO @ Sat, 03 Apr 2021 07:55:11: 43000000 INFO @ Sat, 03 Apr 2021 07:55:15: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:55:15: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:55:15: #1 total tags in treatment: 20762312 INFO @ Sat, 03 Apr 2021 07:55:15: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:55:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:55:15: 38000000 INFO @ Sat, 03 Apr 2021 07:55:16: #1 tags after filtering in treatment: 16635067 INFO @ Sat, 03 Apr 2021 07:55:16: #1 Redundant rate of treatment: 0.20 INFO @ Sat, 03 Apr 2021 07:55:16: #1 finished! INFO @ Sat, 03 Apr 2021 07:55:16: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:55:16: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:55:17: 44000000 INFO @ Sat, 03 Apr 2021 07:55:17: #2 number of paired peaks: 61 WARNING @ Sat, 03 Apr 2021 07:55:17: Too few paired peaks (61) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 07:55:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6430377/SRX6430377.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430377/SRX6430377.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430377/SRX6430377.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430377/SRX6430377.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:55:21: 39000000 INFO @ Sat, 03 Apr 2021 07:55:22: 45000000 INFO @ Sat, 03 Apr 2021 07:55:26: 40000000 INFO @ Sat, 03 Apr 2021 07:55:27: 46000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 07:55:32: 41000000 INFO @ Sat, 03 Apr 2021 07:55:33: 47000000 INFO @ Sat, 03 Apr 2021 07:55:37: 42000000 INFO @ Sat, 03 Apr 2021 07:55:38: 48000000 INFO @ Sat, 03 Apr 2021 07:55:43: 43000000 INFO @ Sat, 03 Apr 2021 07:55:43: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:55:43: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:55:43: #1 total tags in treatment: 20762312 INFO @ Sat, 03 Apr 2021 07:55:43: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:55:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:55:43: #1 tags after filtering in treatment: 16635067 INFO @ Sat, 03 Apr 2021 07:55:43: #1 Redundant rate of treatment: 0.20 INFO @ Sat, 03 Apr 2021 07:55:43: #1 finished! INFO @ Sat, 03 Apr 2021 07:55:43: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:55:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:55:44: #2 number of paired peaks: 61 WARNING @ Sat, 03 Apr 2021 07:55:44: Too few paired peaks (61) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 07:55:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6430377/SRX6430377.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430377/SRX6430377.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430377/SRX6430377.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430377/SRX6430377.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:55:48: 44000000 INFO @ Sat, 03 Apr 2021 07:55:53: 45000000 INFO @ Sat, 03 Apr 2021 07:55:58: 46000000 INFO @ Sat, 03 Apr 2021 07:56:04: 47000000 INFO @ Sat, 03 Apr 2021 07:56:09: 48000000 INFO @ Sat, 03 Apr 2021 07:56:13: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:56:13: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:56:13: #1 total tags in treatment: 20762312 INFO @ Sat, 03 Apr 2021 07:56:13: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:56:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:56:14: #1 tags after filtering in treatment: 16635067 INFO @ Sat, 03 Apr 2021 07:56:14: #1 Redundant rate of treatment: 0.20 INFO @ Sat, 03 Apr 2021 07:56:14: #1 finished! INFO @ Sat, 03 Apr 2021 07:56:14: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:56:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:56:15: #2 number of paired peaks: 61 WARNING @ Sat, 03 Apr 2021 07:56:15: Too few paired peaks (61) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 07:56:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6430377/SRX6430377.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430377/SRX6430377.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430377/SRX6430377.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430377/SRX6430377.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。