Job ID = 12265363 SRX = SRX6430376 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 31477393 spots for SRR9669865/SRR9669865.sra Written 31477393 spots for SRR9669865/SRR9669865.sra fastq に変換しました。 bowtie でマッピング中... Your job 12265701 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:58:38 31477393 reads; of these: 31477393 (100.00%) were paired; of these: 9519346 (30.24%) aligned concordantly 0 times 16828412 (53.46%) aligned concordantly exactly 1 time 5129635 (16.30%) aligned concordantly >1 times ---- 9519346 pairs aligned concordantly 0 times; of these: 3076702 (32.32%) aligned discordantly 1 time ---- 6442644 pairs aligned 0 times concordantly or discordantly; of these: 12885288 mates make up the pairs; of these: 10829278 (84.04%) aligned 0 times 763942 (5.93%) aligned exactly 1 time 1292068 (10.03%) aligned >1 times 82.80% overall alignment rate Time searching: 00:58:38 Overall time: 00:58:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 2148648 / 24671779 = 0.0871 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:59:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6430376/SRX6430376.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6430376/SRX6430376.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6430376/SRX6430376.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6430376/SRX6430376.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:59:23: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:59:23: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:59:32: 1000000 INFO @ Sat, 03 Apr 2021 07:59:40: 2000000 INFO @ Sat, 03 Apr 2021 07:59:48: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:59:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6430376/SRX6430376.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6430376/SRX6430376.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6430376/SRX6430376.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6430376/SRX6430376.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:59:53: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:59:53: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:59:56: 4000000 INFO @ Sat, 03 Apr 2021 08:00:03: 5000000 INFO @ Sat, 03 Apr 2021 08:00:03: 1000000 INFO @ Sat, 03 Apr 2021 08:00:11: 6000000 INFO @ Sat, 03 Apr 2021 08:00:13: 2000000 INFO @ Sat, 03 Apr 2021 08:00:19: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 08:00:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6430376/SRX6430376.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6430376/SRX6430376.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6430376/SRX6430376.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6430376/SRX6430376.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 08:00:23: #1 read tag files... INFO @ Sat, 03 Apr 2021 08:00:23: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 08:00:23: 3000000 INFO @ Sat, 03 Apr 2021 08:00:27: 8000000 INFO @ Sat, 03 Apr 2021 08:00:33: 1000000 INFO @ Sat, 03 Apr 2021 08:00:34: 4000000 INFO @ Sat, 03 Apr 2021 08:00:35: 9000000 INFO @ Sat, 03 Apr 2021 08:00:42: 2000000 INFO @ Sat, 03 Apr 2021 08:00:43: 10000000 INFO @ Sat, 03 Apr 2021 08:00:44: 5000000 INFO @ Sat, 03 Apr 2021 08:00:51: 3000000 INFO @ Sat, 03 Apr 2021 08:00:52: 11000000 INFO @ Sat, 03 Apr 2021 08:00:54: 6000000 INFO @ Sat, 03 Apr 2021 08:01:00: 4000000 INFO @ Sat, 03 Apr 2021 08:01:01: 12000000 INFO @ Sat, 03 Apr 2021 08:01:05: 7000000 INFO @ Sat, 03 Apr 2021 08:01:09: 5000000 INFO @ Sat, 03 Apr 2021 08:01:10: 13000000 INFO @ Sat, 03 Apr 2021 08:01:15: 8000000 INFO @ Sat, 03 Apr 2021 08:01:18: 6000000 INFO @ Sat, 03 Apr 2021 08:01:19: 14000000 INFO @ Sat, 03 Apr 2021 08:01:26: 9000000 INFO @ Sat, 03 Apr 2021 08:01:26: 7000000 INFO @ Sat, 03 Apr 2021 08:01:27: 15000000 INFO @ Sat, 03 Apr 2021 08:01:35: 8000000 INFO @ Sat, 03 Apr 2021 08:01:36: 16000000 INFO @ Sat, 03 Apr 2021 08:01:36: 10000000 INFO @ Sat, 03 Apr 2021 08:01:43: 9000000 INFO @ Sat, 03 Apr 2021 08:01:44: 17000000 INFO @ Sat, 03 Apr 2021 08:01:46: 11000000 INFO @ Sat, 03 Apr 2021 08:01:52: 10000000 INFO @ Sat, 03 Apr 2021 08:01:55: 18000000 INFO @ Sat, 03 Apr 2021 08:01:57: 12000000 INFO @ Sat, 03 Apr 2021 08:02:00: 11000000 INFO @ Sat, 03 Apr 2021 08:02:03: 19000000 INFO @ Sat, 03 Apr 2021 08:02:08: 13000000 INFO @ Sat, 03 Apr 2021 08:02:09: 12000000 INFO @ Sat, 03 Apr 2021 08:02:12: 20000000 INFO @ Sat, 03 Apr 2021 08:02:18: 14000000 INFO @ Sat, 03 Apr 2021 08:02:18: 13000000 INFO @ Sat, 03 Apr 2021 08:02:20: 21000000 INFO @ Sat, 03 Apr 2021 08:02:27: 15000000 INFO @ Sat, 03 Apr 2021 08:02:28: 14000000 INFO @ Sat, 03 Apr 2021 08:02:29: 22000000 INFO @ Sat, 03 Apr 2021 08:02:36: 15000000 INFO @ Sat, 03 Apr 2021 08:02:37: 23000000 INFO @ Sat, 03 Apr 2021 08:02:37: 16000000 INFO @ Sat, 03 Apr 2021 08:02:45: 16000000 INFO @ Sat, 03 Apr 2021 08:02:46: 24000000 INFO @ Sat, 03 Apr 2021 08:02:47: 17000000 INFO @ Sat, 03 Apr 2021 08:02:54: 17000000 INFO @ Sat, 03 Apr 2021 08:02:55: 25000000 INFO @ Sat, 03 Apr 2021 08:02:56: 18000000 INFO @ Sat, 03 Apr 2021 08:03:03: 18000000 INFO @ Sat, 03 Apr 2021 08:03:04: 26000000 INFO @ Sat, 03 Apr 2021 08:03:05: 19000000 INFO @ Sat, 03 Apr 2021 08:03:11: 19000000 INFO @ Sat, 03 Apr 2021 08:03:13: 27000000 INFO @ Sat, 03 Apr 2021 08:03:13: 20000000 INFO @ Sat, 03 Apr 2021 08:03:19: 20000000 INFO @ Sat, 03 Apr 2021 08:03:21: 21000000 INFO @ Sat, 03 Apr 2021 08:03:22: 28000000 INFO @ Sat, 03 Apr 2021 08:03:26: 21000000 INFO @ Sat, 03 Apr 2021 08:03:29: 22000000 INFO @ Sat, 03 Apr 2021 08:03:33: 29000000 INFO @ Sat, 03 Apr 2021 08:03:35: 22000000 INFO @ Sat, 03 Apr 2021 08:03:38: 23000000 INFO @ Sat, 03 Apr 2021 08:03:41: 30000000 INFO @ Sat, 03 Apr 2021 08:03:43: 23000000 INFO @ Sat, 03 Apr 2021 08:03:46: 24000000 INFO @ Sat, 03 Apr 2021 08:03:49: 31000000 INFO @ Sat, 03 Apr 2021 08:03:51: 24000000 INFO @ Sat, 03 Apr 2021 08:03:54: 25000000 INFO @ Sat, 03 Apr 2021 08:03:57: 32000000 INFO @ Sat, 03 Apr 2021 08:03:59: 25000000 INFO @ Sat, 03 Apr 2021 08:04:02: 26000000 INFO @ Sat, 03 Apr 2021 08:04:06: 33000000 INFO @ Sat, 03 Apr 2021 08:04:08: 26000000 INFO @ Sat, 03 Apr 2021 08:04:10: 27000000 INFO @ Sat, 03 Apr 2021 08:04:14: 34000000 INFO @ Sat, 03 Apr 2021 08:04:16: 27000000 INFO @ Sat, 03 Apr 2021 08:04:18: 28000000 INFO @ Sat, 03 Apr 2021 08:04:23: 35000000 INFO @ Sat, 03 Apr 2021 08:04:25: 28000000 INFO @ Sat, 03 Apr 2021 08:04:26: 29000000 INFO @ Sat, 03 Apr 2021 08:04:32: 36000000 INFO @ Sat, 03 Apr 2021 08:04:33: 29000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 08:04:35: 30000000 INFO @ Sat, 03 Apr 2021 08:04:40: 37000000 INFO @ Sat, 03 Apr 2021 08:04:42: 30000000 INFO @ Sat, 03 Apr 2021 08:04:43: 31000000 INFO @ Sat, 03 Apr 2021 08:04:49: 38000000 INFO @ Sat, 03 Apr 2021 08:04:51: 31000000 INFO @ Sat, 03 Apr 2021 08:04:51: 32000000 INFO @ Sat, 03 Apr 2021 08:04:57: 39000000 INFO @ Sat, 03 Apr 2021 08:04:59: 32000000 INFO @ Sat, 03 Apr 2021 08:04:59: 33000000 INFO @ Sat, 03 Apr 2021 08:05:06: 40000000 INFO @ Sat, 03 Apr 2021 08:05:07: 34000000 INFO @ Sat, 03 Apr 2021 08:05:07: 33000000 INFO @ Sat, 03 Apr 2021 08:05:15: 41000000 INFO @ Sat, 03 Apr 2021 08:05:15: 35000000 INFO @ Sat, 03 Apr 2021 08:05:16: 34000000 INFO @ Sat, 03 Apr 2021 08:05:24: 35000000 INFO @ Sat, 03 Apr 2021 08:05:24: 42000000 INFO @ Sat, 03 Apr 2021 08:05:24: 36000000 INFO @ Sat, 03 Apr 2021 08:05:32: 36000000 INFO @ Sat, 03 Apr 2021 08:05:32: 43000000 INFO @ Sat, 03 Apr 2021 08:05:32: 37000000 INFO @ Sat, 03 Apr 2021 08:05:41: 37000000 INFO @ Sat, 03 Apr 2021 08:05:41: 38000000 INFO @ Sat, 03 Apr 2021 08:05:41: 44000000 INFO @ Sat, 03 Apr 2021 08:05:49: 39000000 INFO @ Sat, 03 Apr 2021 08:05:49: 45000000 INFO @ Sat, 03 Apr 2021 08:05:49: 38000000 INFO @ Sat, 03 Apr 2021 08:05:57: 40000000 INFO @ Sat, 03 Apr 2021 08:05:58: 46000000 INFO @ Sat, 03 Apr 2021 08:05:58: 39000000 INFO @ Sat, 03 Apr 2021 08:06:06: 41000000 INFO @ Sat, 03 Apr 2021 08:06:06: 47000000 INFO @ Sat, 03 Apr 2021 08:06:07: 40000000 BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 08:06:14: 42000000 INFO @ Sat, 03 Apr 2021 08:06:14: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 08:06:14: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 08:06:14: #1 total tags in treatment: 20069968 INFO @ Sat, 03 Apr 2021 08:06:14: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:06:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:06:15: #1 tags after filtering in treatment: 15950449 INFO @ Sat, 03 Apr 2021 08:06:15: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 03 Apr 2021 08:06:15: #1 finished! INFO @ Sat, 03 Apr 2021 08:06:15: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:06:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:06:15: 41000000 INFO @ Sat, 03 Apr 2021 08:06:16: #2 number of paired peaks: 70 WARNING @ Sat, 03 Apr 2021 08:06:16: Too few paired peaks (70) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 08:06:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6430376/SRX6430376.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430376/SRX6430376.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430376/SRX6430376.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430376/SRX6430376.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 08:06:22: 43000000 INFO @ Sat, 03 Apr 2021 08:06:23: 42000000 INFO @ Sat, 03 Apr 2021 08:06:30: 44000000 INFO @ Sat, 03 Apr 2021 08:06:30: 43000000 INFO @ Sat, 03 Apr 2021 08:06:38: 44000000 INFO @ Sat, 03 Apr 2021 08:06:39: 45000000 INFO @ Sat, 03 Apr 2021 08:06:46: 45000000 INFO @ Sat, 03 Apr 2021 08:06:47: 46000000 INFO @ Sat, 03 Apr 2021 08:06:53: 46000000 INFO @ Sat, 03 Apr 2021 08:06:55: 47000000 INFO @ Sat, 03 Apr 2021 08:07:01: 47000000 INFO @ Sat, 03 Apr 2021 08:07:02: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 08:07:02: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 08:07:02: #1 total tags in treatment: 20069968 INFO @ Sat, 03 Apr 2021 08:07:02: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:07:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:07:03: #1 tags after filtering in treatment: 15950449 INFO @ Sat, 03 Apr 2021 08:07:03: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 03 Apr 2021 08:07:03: #1 finished! INFO @ Sat, 03 Apr 2021 08:07:03: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:07:03: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:07:04: #2 number of paired peaks: 70 WARNING @ Sat, 03 Apr 2021 08:07:04: Too few paired peaks (70) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 08:07:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6430376/SRX6430376.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430376/SRX6430376.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430376/SRX6430376.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430376/SRX6430376.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 08:07:07: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 08:07:07: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 08:07:07: #1 total tags in treatment: 20069968 INFO @ Sat, 03 Apr 2021 08:07:07: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 08:07:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 08:07:07: #1 tags after filtering in treatment: 15950449 INFO @ Sat, 03 Apr 2021 08:07:07: #1 Redundant rate of treatment: 0.21 INFO @ Sat, 03 Apr 2021 08:07:07: #1 finished! INFO @ Sat, 03 Apr 2021 08:07:07: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 08:07:07: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 08:07:08: #2 number of paired peaks: 70 WARNING @ Sat, 03 Apr 2021 08:07:08: Too few paired peaks (70) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 08:07:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6430376/SRX6430376.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430376/SRX6430376.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430376/SRX6430376.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430376/SRX6430376.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling