Job ID = 12265362 SRX = SRX6430375 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 25031515 spots for SRR9669866/SRR9669866.sra Written 25031515 spots for SRR9669866/SRR9669866.sra fastq に変換しました。 bowtie でマッピング中... Your job 12265637 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:43:35 25031515 reads; of these: 25031515 (100.00%) were paired; of these: 7525038 (30.06%) aligned concordantly 0 times 13521368 (54.02%) aligned concordantly exactly 1 time 3985109 (15.92%) aligned concordantly >1 times ---- 7525038 pairs aligned concordantly 0 times; of these: 2483749 (33.01%) aligned discordantly 1 time ---- 5041289 pairs aligned 0 times concordantly or discordantly; of these: 10082578 mates make up the pairs; of these: 8339474 (82.71%) aligned 0 times 722740 (7.17%) aligned exactly 1 time 1020364 (10.12%) aligned >1 times 83.34% overall alignment rate Time searching: 00:43:35 Overall time: 00:43:35 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 1508961 / 19708000 = 0.0766 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:39:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6430375/SRX6430375.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6430375/SRX6430375.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6430375/SRX6430375.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6430375/SRX6430375.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:39:39: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:39:39: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:39:44: 1000000 INFO @ Sat, 03 Apr 2021 07:39:50: 2000000 INFO @ Sat, 03 Apr 2021 07:39:55: 3000000 INFO @ Sat, 03 Apr 2021 07:40:01: 4000000 INFO @ Sat, 03 Apr 2021 07:40:06: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:40:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6430375/SRX6430375.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6430375/SRX6430375.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6430375/SRX6430375.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6430375/SRX6430375.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:40:09: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:40:09: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:40:12: 6000000 INFO @ Sat, 03 Apr 2021 07:40:15: 1000000 INFO @ Sat, 03 Apr 2021 07:40:18: 7000000 INFO @ Sat, 03 Apr 2021 07:40:21: 2000000 INFO @ Sat, 03 Apr 2021 07:40:24: 8000000 INFO @ Sat, 03 Apr 2021 07:40:27: 3000000 INFO @ Sat, 03 Apr 2021 07:40:29: 9000000 INFO @ Sat, 03 Apr 2021 07:40:33: 4000000 INFO @ Sat, 03 Apr 2021 07:40:35: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:40:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6430375/SRX6430375.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6430375/SRX6430375.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6430375/SRX6430375.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6430375/SRX6430375.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:40:39: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:40:39: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:40:39: 5000000 INFO @ Sat, 03 Apr 2021 07:40:42: 11000000 INFO @ Sat, 03 Apr 2021 07:40:45: 6000000 INFO @ Sat, 03 Apr 2021 07:40:46: 1000000 INFO @ Sat, 03 Apr 2021 07:40:48: 12000000 INFO @ Sat, 03 Apr 2021 07:40:52: 7000000 INFO @ Sat, 03 Apr 2021 07:40:53: 2000000 INFO @ Sat, 03 Apr 2021 07:40:54: 13000000 INFO @ Sat, 03 Apr 2021 07:40:58: 8000000 INFO @ Sat, 03 Apr 2021 07:41:00: 3000000 INFO @ Sat, 03 Apr 2021 07:41:00: 14000000 INFO @ Sat, 03 Apr 2021 07:41:05: 9000000 INFO @ Sat, 03 Apr 2021 07:41:07: 4000000 INFO @ Sat, 03 Apr 2021 07:41:07: 15000000 INFO @ Sat, 03 Apr 2021 07:41:11: 10000000 INFO @ Sat, 03 Apr 2021 07:41:13: 16000000 INFO @ Sat, 03 Apr 2021 07:41:13: 5000000 INFO @ Sat, 03 Apr 2021 07:41:18: 11000000 INFO @ Sat, 03 Apr 2021 07:41:20: 17000000 INFO @ Sat, 03 Apr 2021 07:41:20: 6000000 INFO @ Sat, 03 Apr 2021 07:41:24: 12000000 INFO @ Sat, 03 Apr 2021 07:41:26: 18000000 INFO @ Sat, 03 Apr 2021 07:41:27: 7000000 INFO @ Sat, 03 Apr 2021 07:41:31: 13000000 INFO @ Sat, 03 Apr 2021 07:41:33: 19000000 INFO @ Sat, 03 Apr 2021 07:41:33: 8000000 INFO @ Sat, 03 Apr 2021 07:41:37: 14000000 INFO @ Sat, 03 Apr 2021 07:41:39: 20000000 INFO @ Sat, 03 Apr 2021 07:41:40: 9000000 INFO @ Sat, 03 Apr 2021 07:41:44: 15000000 INFO @ Sat, 03 Apr 2021 07:41:46: 21000000 INFO @ Sat, 03 Apr 2021 07:41:47: 10000000 INFO @ Sat, 03 Apr 2021 07:41:50: 16000000 INFO @ Sat, 03 Apr 2021 07:41:52: 22000000 INFO @ Sat, 03 Apr 2021 07:41:54: 11000000 INFO @ Sat, 03 Apr 2021 07:41:57: 17000000 INFO @ Sat, 03 Apr 2021 07:41:59: 23000000 INFO @ Sat, 03 Apr 2021 07:42:00: 12000000 INFO @ Sat, 03 Apr 2021 07:42:03: 18000000 INFO @ Sat, 03 Apr 2021 07:42:05: 24000000 INFO @ Sat, 03 Apr 2021 07:42:07: 13000000 INFO @ Sat, 03 Apr 2021 07:42:10: 19000000 INFO @ Sat, 03 Apr 2021 07:42:11: 25000000 INFO @ Sat, 03 Apr 2021 07:42:13: 14000000 INFO @ Sat, 03 Apr 2021 07:42:16: 20000000 INFO @ Sat, 03 Apr 2021 07:42:18: 26000000 INFO @ Sat, 03 Apr 2021 07:42:19: 15000000 INFO @ Sat, 03 Apr 2021 07:42:23: 21000000 INFO @ Sat, 03 Apr 2021 07:42:24: 27000000 INFO @ Sat, 03 Apr 2021 07:42:26: 16000000 INFO @ Sat, 03 Apr 2021 07:42:29: 22000000 INFO @ Sat, 03 Apr 2021 07:42:30: 28000000 INFO @ Sat, 03 Apr 2021 07:42:32: 17000000 INFO @ Sat, 03 Apr 2021 07:42:35: 23000000 INFO @ Sat, 03 Apr 2021 07:42:37: 29000000 INFO @ Sat, 03 Apr 2021 07:42:38: 18000000 INFO @ Sat, 03 Apr 2021 07:42:42: 24000000 INFO @ Sat, 03 Apr 2021 07:42:43: 30000000 INFO @ Sat, 03 Apr 2021 07:42:45: 19000000 INFO @ Sat, 03 Apr 2021 07:42:48: 25000000 INFO @ Sat, 03 Apr 2021 07:42:50: 31000000 INFO @ Sat, 03 Apr 2021 07:42:51: 20000000 INFO @ Sat, 03 Apr 2021 07:42:55: 26000000 INFO @ Sat, 03 Apr 2021 07:42:57: 32000000 INFO @ Sat, 03 Apr 2021 07:42:58: 21000000 INFO @ Sat, 03 Apr 2021 07:43:01: 27000000 INFO @ Sat, 03 Apr 2021 07:43:03: 33000000 INFO @ Sat, 03 Apr 2021 07:43:04: 22000000 INFO @ Sat, 03 Apr 2021 07:43:08: 28000000 INFO @ Sat, 03 Apr 2021 07:43:10: 34000000 INFO @ Sat, 03 Apr 2021 07:43:11: 23000000 INFO @ Sat, 03 Apr 2021 07:43:14: 29000000 INFO @ Sat, 03 Apr 2021 07:43:16: 35000000 INFO @ Sat, 03 Apr 2021 07:43:18: 24000000 INFO @ Sat, 03 Apr 2021 07:43:21: 30000000 INFO @ Sat, 03 Apr 2021 07:43:23: 36000000 INFO @ Sat, 03 Apr 2021 07:43:25: 25000000 INFO @ Sat, 03 Apr 2021 07:43:27: 31000000 INFO @ Sat, 03 Apr 2021 07:43:29: 37000000 INFO @ Sat, 03 Apr 2021 07:43:31: 26000000 INFO @ Sat, 03 Apr 2021 07:43:34: 32000000 INFO @ Sat, 03 Apr 2021 07:43:36: 38000000 INFO @ Sat, 03 Apr 2021 07:43:38: 27000000 INFO @ Sat, 03 Apr 2021 07:43:40: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:43:40: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:43:40: #1 total tags in treatment: 16188669 INFO @ Sat, 03 Apr 2021 07:43:40: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:43:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:43:40: 33000000 INFO @ Sat, 03 Apr 2021 07:43:40: #1 tags after filtering in treatment: 13482366 INFO @ Sat, 03 Apr 2021 07:43:40: #1 Redundant rate of treatment: 0.17 INFO @ Sat, 03 Apr 2021 07:43:40: #1 finished! INFO @ Sat, 03 Apr 2021 07:43:40: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:43:40: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:43:41: #2 number of paired peaks: 65 WARNING @ Sat, 03 Apr 2021 07:43:41: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 07:43:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6430375/SRX6430375.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430375/SRX6430375.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430375/SRX6430375.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430375/SRX6430375.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:43:45: 28000000 INFO @ Sat, 03 Apr 2021 07:43:46: 34000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 07:43:52: 35000000 INFO @ Sat, 03 Apr 2021 07:43:53: 29000000 INFO @ Sat, 03 Apr 2021 07:43:59: 36000000 INFO @ Sat, 03 Apr 2021 07:44:00: 30000000 INFO @ Sat, 03 Apr 2021 07:44:05: 37000000 INFO @ Sat, 03 Apr 2021 07:44:08: 31000000 INFO @ Sat, 03 Apr 2021 07:44:11: 38000000 INFO @ Sat, 03 Apr 2021 07:44:15: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:44:15: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:44:15: #1 total tags in treatment: 16188669 INFO @ Sat, 03 Apr 2021 07:44:15: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:44:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:44:15: 32000000 INFO @ Sat, 03 Apr 2021 07:44:16: #1 tags after filtering in treatment: 13482366 INFO @ Sat, 03 Apr 2021 07:44:16: #1 Redundant rate of treatment: 0.17 INFO @ Sat, 03 Apr 2021 07:44:16: #1 finished! INFO @ Sat, 03 Apr 2021 07:44:16: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:44:16: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:44:16: #2 number of paired peaks: 65 WARNING @ Sat, 03 Apr 2021 07:44:16: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 07:44:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6430375/SRX6430375.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430375/SRX6430375.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430375/SRX6430375.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430375/SRX6430375.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:44:23: 33000000 INFO @ Sat, 03 Apr 2021 07:44:30: 34000000 INFO @ Sat, 03 Apr 2021 07:44:37: 35000000 INFO @ Sat, 03 Apr 2021 07:44:44: 36000000 INFO @ Sat, 03 Apr 2021 07:44:51: 37000000 INFO @ Sat, 03 Apr 2021 07:44:59: 38000000 INFO @ Sat, 03 Apr 2021 07:45:04: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:45:04: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:45:04: #1 total tags in treatment: 16188669 INFO @ Sat, 03 Apr 2021 07:45:04: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:45:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:45:04: #1 tags after filtering in treatment: 13482366 INFO @ Sat, 03 Apr 2021 07:45:04: #1 Redundant rate of treatment: 0.17 INFO @ Sat, 03 Apr 2021 07:45:04: #1 finished! INFO @ Sat, 03 Apr 2021 07:45:04: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:45:04: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:45:05: #2 number of paired peaks: 65 WARNING @ Sat, 03 Apr 2021 07:45:05: Too few paired peaks (65) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 07:45:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6430375/SRX6430375.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430375/SRX6430375.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430375/SRX6430375.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430375/SRX6430375.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。