Job ID = 12265354 SRX = SRX6430370 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 32332035 spots for SRR9669871/SRR9669871.sra Written 32332035 spots for SRR9669871/SRR9669871.sra fastq に変換しました。 bowtie でマッピング中... Your job 12265551 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:22:36 32332035 reads; of these: 32332035 (100.00%) were paired; of these: 17252877 (53.36%) aligned concordantly 0 times 9011933 (27.87%) aligned concordantly exactly 1 time 6067225 (18.77%) aligned concordantly >1 times ---- 17252877 pairs aligned concordantly 0 times; of these: 1472582 (8.54%) aligned discordantly 1 time ---- 15780295 pairs aligned 0 times concordantly or discordantly; of these: 31560590 mates make up the pairs; of these: 30050959 (95.22%) aligned 0 times 487804 (1.55%) aligned exactly 1 time 1021827 (3.24%) aligned >1 times 53.53% overall alignment rate Time searching: 00:22:36 Overall time: 00:22:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 3364456 / 16349911 = 0.2058 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:13:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6430370/SRX6430370.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6430370/SRX6430370.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6430370/SRX6430370.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6430370/SRX6430370.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:13:36: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:13:36: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:13:42: 1000000 INFO @ Sat, 03 Apr 2021 07:13:48: 2000000 INFO @ Sat, 03 Apr 2021 07:13:55: 3000000 INFO @ Sat, 03 Apr 2021 07:14:01: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:14:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6430370/SRX6430370.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6430370/SRX6430370.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6430370/SRX6430370.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6430370/SRX6430370.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:14:05: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:14:05: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:14:07: 5000000 INFO @ Sat, 03 Apr 2021 07:14:12: 1000000 INFO @ Sat, 03 Apr 2021 07:14:14: 6000000 INFO @ Sat, 03 Apr 2021 07:14:18: 2000000 INFO @ Sat, 03 Apr 2021 07:14:20: 7000000 INFO @ Sat, 03 Apr 2021 07:14:24: 3000000 INFO @ Sat, 03 Apr 2021 07:14:27: 8000000 INFO @ Sat, 03 Apr 2021 07:14:31: 4000000 INFO @ Sat, 03 Apr 2021 07:14:33: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:14:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6430370/SRX6430370.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6430370/SRX6430370.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6430370/SRX6430370.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6430370/SRX6430370.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:14:35: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:14:35: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:14:37: 5000000 INFO @ Sat, 03 Apr 2021 07:14:40: 10000000 INFO @ Sat, 03 Apr 2021 07:14:42: 1000000 INFO @ Sat, 03 Apr 2021 07:14:43: 6000000 INFO @ Sat, 03 Apr 2021 07:14:47: 11000000 INFO @ Sat, 03 Apr 2021 07:14:48: 2000000 INFO @ Sat, 03 Apr 2021 07:14:49: 7000000 INFO @ Sat, 03 Apr 2021 07:14:53: 12000000 INFO @ Sat, 03 Apr 2021 07:14:54: 3000000 INFO @ Sat, 03 Apr 2021 07:14:56: 8000000 INFO @ Sat, 03 Apr 2021 07:15:00: 13000000 INFO @ Sat, 03 Apr 2021 07:15:01: 4000000 INFO @ Sat, 03 Apr 2021 07:15:02: 9000000 INFO @ Sat, 03 Apr 2021 07:15:06: 14000000 INFO @ Sat, 03 Apr 2021 07:15:07: 5000000 INFO @ Sat, 03 Apr 2021 07:15:08: 10000000 INFO @ Sat, 03 Apr 2021 07:15:13: 15000000 INFO @ Sat, 03 Apr 2021 07:15:14: 6000000 INFO @ Sat, 03 Apr 2021 07:15:15: 11000000 INFO @ Sat, 03 Apr 2021 07:15:20: 16000000 INFO @ Sat, 03 Apr 2021 07:15:20: 7000000 INFO @ Sat, 03 Apr 2021 07:15:21: 12000000 INFO @ Sat, 03 Apr 2021 07:15:26: 17000000 INFO @ Sat, 03 Apr 2021 07:15:26: 8000000 INFO @ Sat, 03 Apr 2021 07:15:28: 13000000 INFO @ Sat, 03 Apr 2021 07:15:33: 9000000 INFO @ Sat, 03 Apr 2021 07:15:33: 18000000 INFO @ Sat, 03 Apr 2021 07:15:34: 14000000 INFO @ Sat, 03 Apr 2021 07:15:39: 10000000 INFO @ Sat, 03 Apr 2021 07:15:40: 19000000 INFO @ Sat, 03 Apr 2021 07:15:41: 15000000 INFO @ Sat, 03 Apr 2021 07:15:45: 11000000 INFO @ Sat, 03 Apr 2021 07:15:46: 20000000 INFO @ Sat, 03 Apr 2021 07:15:47: 16000000 INFO @ Sat, 03 Apr 2021 07:15:52: 12000000 INFO @ Sat, 03 Apr 2021 07:15:53: 21000000 INFO @ Sat, 03 Apr 2021 07:15:53: 17000000 INFO @ Sat, 03 Apr 2021 07:15:58: 13000000 INFO @ Sat, 03 Apr 2021 07:16:00: 22000000 INFO @ Sat, 03 Apr 2021 07:16:00: 18000000 INFO @ Sat, 03 Apr 2021 07:16:04: 14000000 INFO @ Sat, 03 Apr 2021 07:16:06: 19000000 INFO @ Sat, 03 Apr 2021 07:16:06: 23000000 INFO @ Sat, 03 Apr 2021 07:16:10: 15000000 INFO @ Sat, 03 Apr 2021 07:16:12: 20000000 INFO @ Sat, 03 Apr 2021 07:16:13: 24000000 INFO @ Sat, 03 Apr 2021 07:16:17: 16000000 INFO @ Sat, 03 Apr 2021 07:16:19: 21000000 INFO @ Sat, 03 Apr 2021 07:16:20: 25000000 INFO @ Sat, 03 Apr 2021 07:16:23: 17000000 INFO @ Sat, 03 Apr 2021 07:16:25: 22000000 INFO @ Sat, 03 Apr 2021 07:16:26: 26000000 INFO @ Sat, 03 Apr 2021 07:16:29: 18000000 INFO @ Sat, 03 Apr 2021 07:16:31: 23000000 INFO @ Sat, 03 Apr 2021 07:16:33: 27000000 INFO @ Sat, 03 Apr 2021 07:16:35: 19000000 INFO @ Sat, 03 Apr 2021 07:16:38: 24000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 07:16:39: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:16:39: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:16:39: #1 total tags in treatment: 11766295 INFO @ Sat, 03 Apr 2021 07:16:39: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:16:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:16:39: #1 tags after filtering in treatment: 9641016 INFO @ Sat, 03 Apr 2021 07:16:39: #1 Redundant rate of treatment: 0.18 INFO @ Sat, 03 Apr 2021 07:16:39: #1 finished! INFO @ Sat, 03 Apr 2021 07:16:39: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:16:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:16:40: #2 number of paired peaks: 93 WARNING @ Sat, 03 Apr 2021 07:16:40: Too few paired peaks (93) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 07:16:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6430370/SRX6430370.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430370/SRX6430370.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430370/SRX6430370.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430370/SRX6430370.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:16:42: 20000000 INFO @ Sat, 03 Apr 2021 07:16:44: 25000000 INFO @ Sat, 03 Apr 2021 07:16:48: 21000000 INFO @ Sat, 03 Apr 2021 07:16:51: 26000000 INFO @ Sat, 03 Apr 2021 07:16:54: 22000000 INFO @ Sat, 03 Apr 2021 07:16:57: 27000000 INFO @ Sat, 03 Apr 2021 07:17:01: 23000000 INFO @ Sat, 03 Apr 2021 07:17:03: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:17:03: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:17:03: #1 total tags in treatment: 11766295 INFO @ Sat, 03 Apr 2021 07:17:03: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:17:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:17:03: #1 tags after filtering in treatment: 9641016 INFO @ Sat, 03 Apr 2021 07:17:03: #1 Redundant rate of treatment: 0.18 INFO @ Sat, 03 Apr 2021 07:17:03: #1 finished! INFO @ Sat, 03 Apr 2021 07:17:03: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:17:03: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:17:04: #2 number of paired peaks: 93 WARNING @ Sat, 03 Apr 2021 07:17:04: Too few paired peaks (93) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 07:17:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6430370/SRX6430370.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430370/SRX6430370.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430370/SRX6430370.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430370/SRX6430370.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:17:07: 24000000 INFO @ Sat, 03 Apr 2021 07:17:12: 25000000 INFO @ Sat, 03 Apr 2021 07:17:18: 26000000 INFO @ Sat, 03 Apr 2021 07:17:24: 27000000 INFO @ Sat, 03 Apr 2021 07:17:29: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:17:29: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:17:29: #1 total tags in treatment: 11766295 INFO @ Sat, 03 Apr 2021 07:17:29: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:17:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:17:29: #1 tags after filtering in treatment: 9641016 INFO @ Sat, 03 Apr 2021 07:17:29: #1 Redundant rate of treatment: 0.18 INFO @ Sat, 03 Apr 2021 07:17:29: #1 finished! INFO @ Sat, 03 Apr 2021 07:17:29: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:17:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:17:30: #2 number of paired peaks: 93 WARNING @ Sat, 03 Apr 2021 07:17:30: Too few paired peaks (93) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 07:17:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6430370/SRX6430370.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430370/SRX6430370.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430370/SRX6430370.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430370/SRX6430370.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。