Job ID = 12265341 SRX = SRX6430356 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 32449214 spots for SRR9669885/SRR9669885.sra Written 32449214 spots for SRR9669885/SRR9669885.sra fastq に変換しました。 bowtie でマッピング中... Your job 12265633 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:43:41 32449214 reads; of these: 32449214 (100.00%) were paired; of these: 10911527 (33.63%) aligned concordantly 0 times 16638371 (51.28%) aligned concordantly exactly 1 time 4899316 (15.10%) aligned concordantly >1 times ---- 10911527 pairs aligned concordantly 0 times; of these: 3934412 (36.06%) aligned discordantly 1 time ---- 6977115 pairs aligned 0 times concordantly or discordantly; of these: 13954230 mates make up the pairs; of these: 10996759 (78.81%) aligned 0 times 1378925 (9.88%) aligned exactly 1 time 1578546 (11.31%) aligned >1 times 83.06% overall alignment rate Time searching: 00:43:42 Overall time: 00:43:42 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 3070979 / 25064077 = 0.1225 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:42:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6430356/SRX6430356.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6430356/SRX6430356.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6430356/SRX6430356.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6430356/SRX6430356.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:42:45: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:42:45: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:42:52: 1000000 INFO @ Sat, 03 Apr 2021 07:42:59: 2000000 INFO @ Sat, 03 Apr 2021 07:43:06: 3000000 INFO @ Sat, 03 Apr 2021 07:43:12: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:43:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6430356/SRX6430356.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6430356/SRX6430356.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6430356/SRX6430356.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6430356/SRX6430356.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:43:16: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:43:16: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:43:19: 5000000 INFO @ Sat, 03 Apr 2021 07:43:24: 1000000 INFO @ Sat, 03 Apr 2021 07:43:26: 6000000 INFO @ Sat, 03 Apr 2021 07:43:33: 2000000 INFO @ Sat, 03 Apr 2021 07:43:33: 7000000 INFO @ Sat, 03 Apr 2021 07:43:40: 8000000 INFO @ Sat, 03 Apr 2021 07:43:41: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:43:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6430356/SRX6430356.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6430356/SRX6430356.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6430356/SRX6430356.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6430356/SRX6430356.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:43:45: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:43:45: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:43:47: 9000000 INFO @ Sat, 03 Apr 2021 07:43:50: 4000000 INFO @ Sat, 03 Apr 2021 07:43:54: 1000000 INFO @ Sat, 03 Apr 2021 07:43:54: 10000000 INFO @ Sat, 03 Apr 2021 07:43:59: 5000000 INFO @ Sat, 03 Apr 2021 07:44:01: 11000000 INFO @ Sat, 03 Apr 2021 07:44:02: 2000000 INFO @ Sat, 03 Apr 2021 07:44:08: 12000000 INFO @ Sat, 03 Apr 2021 07:44:08: 6000000 INFO @ Sat, 03 Apr 2021 07:44:10: 3000000 INFO @ Sat, 03 Apr 2021 07:44:15: 13000000 INFO @ Sat, 03 Apr 2021 07:44:17: 7000000 INFO @ Sat, 03 Apr 2021 07:44:18: 4000000 INFO @ Sat, 03 Apr 2021 07:44:23: 14000000 INFO @ Sat, 03 Apr 2021 07:44:26: 8000000 INFO @ Sat, 03 Apr 2021 07:44:26: 5000000 INFO @ Sat, 03 Apr 2021 07:44:30: 15000000 INFO @ Sat, 03 Apr 2021 07:44:34: 6000000 INFO @ Sat, 03 Apr 2021 07:44:34: 9000000 INFO @ Sat, 03 Apr 2021 07:44:36: 16000000 INFO @ Sat, 03 Apr 2021 07:44:42: 7000000 INFO @ Sat, 03 Apr 2021 07:44:43: 10000000 INFO @ Sat, 03 Apr 2021 07:44:43: 17000000 INFO @ Sat, 03 Apr 2021 07:44:50: 8000000 INFO @ Sat, 03 Apr 2021 07:44:50: 18000000 INFO @ Sat, 03 Apr 2021 07:44:52: 11000000 INFO @ Sat, 03 Apr 2021 07:44:57: 9000000 INFO @ Sat, 03 Apr 2021 07:44:58: 19000000 INFO @ Sat, 03 Apr 2021 07:45:00: 12000000 INFO @ Sat, 03 Apr 2021 07:45:05: 10000000 INFO @ Sat, 03 Apr 2021 07:45:06: 20000000 INFO @ Sat, 03 Apr 2021 07:45:09: 13000000 INFO @ Sat, 03 Apr 2021 07:45:13: 11000000 INFO @ Sat, 03 Apr 2021 07:45:15: 21000000 INFO @ Sat, 03 Apr 2021 07:45:18: 14000000 INFO @ Sat, 03 Apr 2021 07:45:21: 12000000 INFO @ Sat, 03 Apr 2021 07:45:25: 22000000 INFO @ Sat, 03 Apr 2021 07:45:26: 15000000 INFO @ Sat, 03 Apr 2021 07:45:29: 13000000 INFO @ Sat, 03 Apr 2021 07:45:35: 16000000 INFO @ Sat, 03 Apr 2021 07:45:35: 23000000 INFO @ Sat, 03 Apr 2021 07:45:37: 14000000 INFO @ Sat, 03 Apr 2021 07:45:44: 17000000 INFO @ Sat, 03 Apr 2021 07:45:45: 15000000 INFO @ Sat, 03 Apr 2021 07:45:46: 24000000 INFO @ Sat, 03 Apr 2021 07:45:52: 18000000 INFO @ Sat, 03 Apr 2021 07:45:53: 16000000 INFO @ Sat, 03 Apr 2021 07:45:56: 25000000 INFO @ Sat, 03 Apr 2021 07:46:01: 17000000 INFO @ Sat, 03 Apr 2021 07:46:01: 19000000 INFO @ Sat, 03 Apr 2021 07:46:06: 26000000 INFO @ Sat, 03 Apr 2021 07:46:09: 18000000 INFO @ Sat, 03 Apr 2021 07:46:09: 20000000 INFO @ Sat, 03 Apr 2021 07:46:15: 27000000 INFO @ Sat, 03 Apr 2021 07:46:17: 19000000 INFO @ Sat, 03 Apr 2021 07:46:18: 21000000 INFO @ Sat, 03 Apr 2021 07:46:24: 20000000 INFO @ Sat, 03 Apr 2021 07:46:25: 28000000 INFO @ Sat, 03 Apr 2021 07:46:27: 22000000 INFO @ Sat, 03 Apr 2021 07:46:32: 21000000 INFO @ Sat, 03 Apr 2021 07:46:35: 29000000 INFO @ Sat, 03 Apr 2021 07:46:36: 23000000 INFO @ Sat, 03 Apr 2021 07:46:40: 22000000 INFO @ Sat, 03 Apr 2021 07:46:44: 30000000 INFO @ Sat, 03 Apr 2021 07:46:45: 24000000 INFO @ Sat, 03 Apr 2021 07:46:49: 23000000 INFO @ Sat, 03 Apr 2021 07:46:53: 31000000 INFO @ Sat, 03 Apr 2021 07:46:54: 25000000 INFO @ Sat, 03 Apr 2021 07:46:58: 24000000 INFO @ Sat, 03 Apr 2021 07:47:02: 32000000 INFO @ Sat, 03 Apr 2021 07:47:03: 26000000 INFO @ Sat, 03 Apr 2021 07:47:08: 25000000 INFO @ Sat, 03 Apr 2021 07:47:11: 27000000 INFO @ Sat, 03 Apr 2021 07:47:11: 33000000 INFO @ Sat, 03 Apr 2021 07:47:17: 26000000 INFO @ Sat, 03 Apr 2021 07:47:18: 28000000 INFO @ Sat, 03 Apr 2021 07:47:21: 34000000 INFO @ Sat, 03 Apr 2021 07:47:26: 29000000 INFO @ Sat, 03 Apr 2021 07:47:27: 27000000 INFO @ Sat, 03 Apr 2021 07:47:30: 35000000 INFO @ Sat, 03 Apr 2021 07:47:34: 30000000 INFO @ Sat, 03 Apr 2021 07:47:36: 28000000 INFO @ Sat, 03 Apr 2021 07:47:40: 36000000 INFO @ Sat, 03 Apr 2021 07:47:42: 31000000 INFO @ Sat, 03 Apr 2021 07:47:45: 29000000 INFO @ Sat, 03 Apr 2021 07:47:48: 37000000 INFO @ Sat, 03 Apr 2021 07:47:50: 32000000 INFO @ Sat, 03 Apr 2021 07:47:54: 30000000 INFO @ Sat, 03 Apr 2021 07:47:55: 38000000 INFO @ Sat, 03 Apr 2021 07:47:57: 33000000 INFO @ Sat, 03 Apr 2021 07:48:02: 39000000 INFO @ Sat, 03 Apr 2021 07:48:02: 31000000 INFO @ Sat, 03 Apr 2021 07:48:06: 34000000 INFO @ Sat, 03 Apr 2021 07:48:11: 40000000 INFO @ Sat, 03 Apr 2021 07:48:11: 32000000 INFO @ Sat, 03 Apr 2021 07:48:15: 35000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 07:48:20: 41000000 INFO @ Sat, 03 Apr 2021 07:48:20: 33000000 INFO @ Sat, 03 Apr 2021 07:48:23: 36000000 INFO @ Sat, 03 Apr 2021 07:48:28: 42000000 INFO @ Sat, 03 Apr 2021 07:48:29: 34000000 INFO @ Sat, 03 Apr 2021 07:48:31: 37000000 INFO @ Sat, 03 Apr 2021 07:48:37: 43000000 INFO @ Sat, 03 Apr 2021 07:48:38: 35000000 INFO @ Sat, 03 Apr 2021 07:48:39: 38000000 INFO @ Sat, 03 Apr 2021 07:48:45: 44000000 INFO @ Sat, 03 Apr 2021 07:48:46: 39000000 INFO @ Sat, 03 Apr 2021 07:48:47: 36000000 INFO @ Sat, 03 Apr 2021 07:48:54: 40000000 INFO @ Sat, 03 Apr 2021 07:48:54: 45000000 INFO @ Sat, 03 Apr 2021 07:48:56: 37000000 INFO @ Sat, 03 Apr 2021 07:49:01: 41000000 INFO @ Sat, 03 Apr 2021 07:49:03: 46000000 INFO @ Sat, 03 Apr 2021 07:49:05: 38000000 INFO @ Sat, 03 Apr 2021 07:49:09: 42000000 INFO @ Sat, 03 Apr 2021 07:49:13: 47000000 INFO @ Sat, 03 Apr 2021 07:49:14: 39000000 INFO @ Sat, 03 Apr 2021 07:49:16: 43000000 INFO @ Sat, 03 Apr 2021 07:49:21: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:49:21: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:49:21: #1 total tags in treatment: 18841272 INFO @ Sat, 03 Apr 2021 07:49:21: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:49:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:49:21: #1 tags after filtering in treatment: 15351916 INFO @ Sat, 03 Apr 2021 07:49:21: #1 Redundant rate of treatment: 0.19 INFO @ Sat, 03 Apr 2021 07:49:21: #1 finished! INFO @ Sat, 03 Apr 2021 07:49:21: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:49:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:49:23: #2 number of paired peaks: 86 WARNING @ Sat, 03 Apr 2021 07:49:23: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 07:49:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6430356/SRX6430356.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430356/SRX6430356.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430356/SRX6430356.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430356/SRX6430356.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:49:23: 40000000 INFO @ Sat, 03 Apr 2021 07:49:24: 44000000 INFO @ Sat, 03 Apr 2021 07:49:31: 45000000 INFO @ Sat, 03 Apr 2021 07:49:31: 41000000 INFO @ Sat, 03 Apr 2021 07:49:38: 46000000 INFO @ Sat, 03 Apr 2021 07:49:39: 42000000 INFO @ Sat, 03 Apr 2021 07:49:46: 47000000 INFO @ Sat, 03 Apr 2021 07:49:47: 43000000 INFO @ Sat, 03 Apr 2021 07:49:52: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:49:52: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:49:52: #1 total tags in treatment: 18841272 INFO @ Sat, 03 Apr 2021 07:49:52: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:49:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:49:53: #1 tags after filtering in treatment: 15351916 INFO @ Sat, 03 Apr 2021 07:49:53: #1 Redundant rate of treatment: 0.19 INFO @ Sat, 03 Apr 2021 07:49:53: #1 finished! INFO @ Sat, 03 Apr 2021 07:49:53: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:49:53: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:49:54: #2 number of paired peaks: 86 WARNING @ Sat, 03 Apr 2021 07:49:54: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 07:49:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6430356/SRX6430356.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430356/SRX6430356.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430356/SRX6430356.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430356/SRX6430356.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:49:55: 44000000 INFO @ Sat, 03 Apr 2021 07:50:03: 45000000 BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 07:50:11: 46000000 INFO @ Sat, 03 Apr 2021 07:50:18: 47000000 INFO @ Sat, 03 Apr 2021 07:50:24: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:50:24: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:50:24: #1 total tags in treatment: 18841272 INFO @ Sat, 03 Apr 2021 07:50:24: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:50:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:50:25: #1 tags after filtering in treatment: 15351916 INFO @ Sat, 03 Apr 2021 07:50:25: #1 Redundant rate of treatment: 0.19 INFO @ Sat, 03 Apr 2021 07:50:25: #1 finished! INFO @ Sat, 03 Apr 2021 07:50:25: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:50:25: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:50:26: #2 number of paired peaks: 86 WARNING @ Sat, 03 Apr 2021 07:50:26: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 07:50:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6430356/SRX6430356.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430356/SRX6430356.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430356/SRX6430356.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430356/SRX6430356.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling