Job ID = 12265340 SRX = SRX6430355 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 33786399 spots for SRR9669886/SRR9669886.sra Written 33786399 spots for SRR9669886/SRR9669886.sra fastq に変換しました。 bowtie でマッピング中... Your job 12265626 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:41:26 33786399 reads; of these: 33786399 (100.00%) were paired; of these: 7729213 (22.88%) aligned concordantly 0 times 18667890 (55.25%) aligned concordantly exactly 1 time 7389296 (21.87%) aligned concordantly >1 times ---- 7729213 pairs aligned concordantly 0 times; of these: 3580424 (46.32%) aligned discordantly 1 time ---- 4148789 pairs aligned 0 times concordantly or discordantly; of these: 8297578 mates make up the pairs; of these: 5609348 (67.60%) aligned 0 times 1084643 (13.07%) aligned exactly 1 time 1603587 (19.33%) aligned >1 times 91.70% overall alignment rate Time searching: 00:41:26 Overall time: 00:41:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 28 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 4985222 / 29215942 = 0.1706 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:42:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6430355/SRX6430355.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6430355/SRX6430355.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6430355/SRX6430355.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6430355/SRX6430355.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:42:27: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:42:27: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:42:34: 1000000 INFO @ Sat, 03 Apr 2021 07:42:42: 2000000 INFO @ Sat, 03 Apr 2021 07:42:50: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:42:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6430355/SRX6430355.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6430355/SRX6430355.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6430355/SRX6430355.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6430355/SRX6430355.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:42:57: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:42:57: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:42:58: 4000000 INFO @ Sat, 03 Apr 2021 07:43:04: 1000000 INFO @ Sat, 03 Apr 2021 07:43:06: 5000000 INFO @ Sat, 03 Apr 2021 07:43:11: 2000000 INFO @ Sat, 03 Apr 2021 07:43:15: 6000000 INFO @ Sat, 03 Apr 2021 07:43:19: 3000000 INFO @ Sat, 03 Apr 2021 07:43:23: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:43:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6430355/SRX6430355.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6430355/SRX6430355.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6430355/SRX6430355.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6430355/SRX6430355.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:43:26: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:43:26: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:43:28: 4000000 INFO @ Sat, 03 Apr 2021 07:43:32: 8000000 INFO @ Sat, 03 Apr 2021 07:43:35: 1000000 INFO @ Sat, 03 Apr 2021 07:43:36: 5000000 INFO @ Sat, 03 Apr 2021 07:43:41: 9000000 INFO @ Sat, 03 Apr 2021 07:43:44: 2000000 INFO @ Sat, 03 Apr 2021 07:43:44: 6000000 INFO @ Sat, 03 Apr 2021 07:43:49: 10000000 INFO @ Sat, 03 Apr 2021 07:43:52: 3000000 INFO @ Sat, 03 Apr 2021 07:43:53: 7000000 INFO @ Sat, 03 Apr 2021 07:43:58: 11000000 INFO @ Sat, 03 Apr 2021 07:44:01: 4000000 INFO @ Sat, 03 Apr 2021 07:44:01: 8000000 INFO @ Sat, 03 Apr 2021 07:44:06: 12000000 INFO @ Sat, 03 Apr 2021 07:44:09: 5000000 INFO @ Sat, 03 Apr 2021 07:44:10: 9000000 INFO @ Sat, 03 Apr 2021 07:44:15: 13000000 INFO @ Sat, 03 Apr 2021 07:44:18: 6000000 INFO @ Sat, 03 Apr 2021 07:44:18: 10000000 INFO @ Sat, 03 Apr 2021 07:44:25: 14000000 INFO @ Sat, 03 Apr 2021 07:44:26: 7000000 INFO @ Sat, 03 Apr 2021 07:44:27: 11000000 INFO @ Sat, 03 Apr 2021 07:44:33: 15000000 INFO @ Sat, 03 Apr 2021 07:44:35: 8000000 INFO @ Sat, 03 Apr 2021 07:44:35: 12000000 INFO @ Sat, 03 Apr 2021 07:44:42: 16000000 INFO @ Sat, 03 Apr 2021 07:44:43: 9000000 INFO @ Sat, 03 Apr 2021 07:44:43: 13000000 INFO @ Sat, 03 Apr 2021 07:44:51: 17000000 INFO @ Sat, 03 Apr 2021 07:44:51: 10000000 INFO @ Sat, 03 Apr 2021 07:44:52: 14000000 INFO @ Sat, 03 Apr 2021 07:44:59: 18000000 INFO @ Sat, 03 Apr 2021 07:45:00: 11000000 INFO @ Sat, 03 Apr 2021 07:45:00: 15000000 INFO @ Sat, 03 Apr 2021 07:45:08: 19000000 INFO @ Sat, 03 Apr 2021 07:45:08: 12000000 INFO @ Sat, 03 Apr 2021 07:45:08: 16000000 INFO @ Sat, 03 Apr 2021 07:45:16: 13000000 INFO @ Sat, 03 Apr 2021 07:45:16: 17000000 INFO @ Sat, 03 Apr 2021 07:45:17: 20000000 INFO @ Sat, 03 Apr 2021 07:45:24: 18000000 INFO @ Sat, 03 Apr 2021 07:45:24: 14000000 INFO @ Sat, 03 Apr 2021 07:45:25: 21000000 INFO @ Sat, 03 Apr 2021 07:45:33: 19000000 INFO @ Sat, 03 Apr 2021 07:45:33: 15000000 INFO @ Sat, 03 Apr 2021 07:45:34: 22000000 INFO @ Sat, 03 Apr 2021 07:45:41: 20000000 INFO @ Sat, 03 Apr 2021 07:45:41: 16000000 INFO @ Sat, 03 Apr 2021 07:45:43: 23000000 INFO @ Sat, 03 Apr 2021 07:45:49: 21000000 INFO @ Sat, 03 Apr 2021 07:45:49: 17000000 INFO @ Sat, 03 Apr 2021 07:45:51: 24000000 INFO @ Sat, 03 Apr 2021 07:45:57: 22000000 INFO @ Sat, 03 Apr 2021 07:45:57: 18000000 INFO @ Sat, 03 Apr 2021 07:46:00: 25000000 INFO @ Sat, 03 Apr 2021 07:46:05: 23000000 INFO @ Sat, 03 Apr 2021 07:46:06: 19000000 INFO @ Sat, 03 Apr 2021 07:46:08: 26000000 INFO @ Sat, 03 Apr 2021 07:46:13: 24000000 INFO @ Sat, 03 Apr 2021 07:46:14: 20000000 INFO @ Sat, 03 Apr 2021 07:46:17: 27000000 INFO @ Sat, 03 Apr 2021 07:46:22: 25000000 INFO @ Sat, 03 Apr 2021 07:46:23: 21000000 INFO @ Sat, 03 Apr 2021 07:46:25: 28000000 INFO @ Sat, 03 Apr 2021 07:46:30: 26000000 INFO @ Sat, 03 Apr 2021 07:46:31: 22000000 INFO @ Sat, 03 Apr 2021 07:46:34: 29000000 INFO @ Sat, 03 Apr 2021 07:46:39: 27000000 INFO @ Sat, 03 Apr 2021 07:46:39: 23000000 INFO @ Sat, 03 Apr 2021 07:46:42: 30000000 INFO @ Sat, 03 Apr 2021 07:46:47: 28000000 INFO @ Sat, 03 Apr 2021 07:46:47: 24000000 INFO @ Sat, 03 Apr 2021 07:46:51: 31000000 INFO @ Sat, 03 Apr 2021 07:46:56: 25000000 INFO @ Sat, 03 Apr 2021 07:46:56: 29000000 INFO @ Sat, 03 Apr 2021 07:47:00: 32000000 INFO @ Sat, 03 Apr 2021 07:47:04: 26000000 INFO @ Sat, 03 Apr 2021 07:47:04: 30000000 INFO @ Sat, 03 Apr 2021 07:47:09: 33000000 INFO @ Sat, 03 Apr 2021 07:47:13: 27000000 INFO @ Sat, 03 Apr 2021 07:47:14: 31000000 INFO @ Sat, 03 Apr 2021 07:47:19: 34000000 INFO @ Sat, 03 Apr 2021 07:47:22: 28000000 INFO @ Sat, 03 Apr 2021 07:47:24: 32000000 INFO @ Sat, 03 Apr 2021 07:47:28: 35000000 INFO @ Sat, 03 Apr 2021 07:47:31: 29000000 INFO @ Sat, 03 Apr 2021 07:47:33: 33000000 INFO @ Sat, 03 Apr 2021 07:47:37: 36000000 INFO @ Sat, 03 Apr 2021 07:47:40: 30000000 INFO @ Sat, 03 Apr 2021 07:47:43: 34000000 INFO @ Sat, 03 Apr 2021 07:47:46: 37000000 INFO @ Sat, 03 Apr 2021 07:47:48: 31000000 INFO @ Sat, 03 Apr 2021 07:47:52: 35000000 INFO @ Sat, 03 Apr 2021 07:47:54: 38000000 INFO @ Sat, 03 Apr 2021 07:47:57: 32000000 INFO @ Sat, 03 Apr 2021 07:48:00: 36000000 INFO @ Sat, 03 Apr 2021 07:48:02: 39000000 INFO @ Sat, 03 Apr 2021 07:48:06: 33000000 INFO @ Sat, 03 Apr 2021 07:48:08: 37000000 INFO @ Sat, 03 Apr 2021 07:48:10: 40000000 INFO @ Sat, 03 Apr 2021 07:48:14: 34000000 INFO @ Sat, 03 Apr 2021 07:48:16: 38000000 INFO @ Sat, 03 Apr 2021 07:48:18: 41000000 INFO @ Sat, 03 Apr 2021 07:48:23: 35000000 INFO @ Sat, 03 Apr 2021 07:48:24: 39000000 INFO @ Sat, 03 Apr 2021 07:48:27: 42000000 INFO @ Sat, 03 Apr 2021 07:48:31: 36000000 INFO @ Sat, 03 Apr 2021 07:48:33: 40000000 INFO @ Sat, 03 Apr 2021 07:48:35: 43000000 INFO @ Sat, 03 Apr 2021 07:48:40: 37000000 INFO @ Sat, 03 Apr 2021 07:48:42: 41000000 INFO @ Sat, 03 Apr 2021 07:48:43: 44000000 INFO @ Sat, 03 Apr 2021 07:48:48: 38000000 INFO @ Sat, 03 Apr 2021 07:48:51: 42000000 INFO @ Sat, 03 Apr 2021 07:48:52: 45000000 INFO @ Sat, 03 Apr 2021 07:48:56: 39000000 INFO @ Sat, 03 Apr 2021 07:49:00: 43000000 INFO @ Sat, 03 Apr 2021 07:49:00: 46000000 INFO @ Sat, 03 Apr 2021 07:49:04: 40000000 INFO @ Sat, 03 Apr 2021 07:49:08: 47000000 INFO @ Sat, 03 Apr 2021 07:49:09: 44000000 INFO @ Sat, 03 Apr 2021 07:49:12: 41000000 INFO @ Sat, 03 Apr 2021 07:49:17: 48000000 INFO @ Sat, 03 Apr 2021 07:49:18: 45000000 INFO @ Sat, 03 Apr 2021 07:49:20: 42000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 07:49:25: 49000000 INFO @ Sat, 03 Apr 2021 07:49:26: 46000000 INFO @ Sat, 03 Apr 2021 07:49:28: 43000000 INFO @ Sat, 03 Apr 2021 07:49:33: 50000000 INFO @ Sat, 03 Apr 2021 07:49:34: 47000000 INFO @ Sat, 03 Apr 2021 07:49:36: 44000000 INFO @ Sat, 03 Apr 2021 07:49:41: 51000000 INFO @ Sat, 03 Apr 2021 07:49:42: 48000000 INFO @ Sat, 03 Apr 2021 07:49:43: 45000000 INFO @ Sat, 03 Apr 2021 07:49:50: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:49:50: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:49:50: #1 total tags in treatment: 21303310 INFO @ Sat, 03 Apr 2021 07:49:50: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:49:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:49:50: 49000000 INFO @ Sat, 03 Apr 2021 07:49:50: #1 tags after filtering in treatment: 16619755 INFO @ Sat, 03 Apr 2021 07:49:50: #1 Redundant rate of treatment: 0.22 INFO @ Sat, 03 Apr 2021 07:49:50: #1 finished! INFO @ Sat, 03 Apr 2021 07:49:50: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:49:50: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:49:51: 46000000 INFO @ Sat, 03 Apr 2021 07:49:52: #2 number of paired peaks: 91 WARNING @ Sat, 03 Apr 2021 07:49:52: Too few paired peaks (91) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 07:49:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6430355/SRX6430355.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430355/SRX6430355.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430355/SRX6430355.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430355/SRX6430355.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:49:58: 50000000 INFO @ Sat, 03 Apr 2021 07:49:59: 47000000 INFO @ Sat, 03 Apr 2021 07:50:05: 51000000 INFO @ Sat, 03 Apr 2021 07:50:06: 48000000 INFO @ Sat, 03 Apr 2021 07:50:13: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:50:13: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:50:13: #1 total tags in treatment: 21303310 INFO @ Sat, 03 Apr 2021 07:50:13: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:50:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:50:14: #1 tags after filtering in treatment: 16619755 INFO @ Sat, 03 Apr 2021 07:50:14: #1 Redundant rate of treatment: 0.22 INFO @ Sat, 03 Apr 2021 07:50:14: #1 finished! INFO @ Sat, 03 Apr 2021 07:50:14: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:50:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:50:14: 49000000 INFO @ Sat, 03 Apr 2021 07:50:15: #2 number of paired peaks: 91 WARNING @ Sat, 03 Apr 2021 07:50:15: Too few paired peaks (91) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 07:50:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6430355/SRX6430355.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430355/SRX6430355.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430355/SRX6430355.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430355/SRX6430355.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:50:21: 50000000 INFO @ Sat, 03 Apr 2021 07:50:29: 51000000 INFO @ Sat, 03 Apr 2021 07:50:37: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:50:37: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:50:37: #1 total tags in treatment: 21303310 INFO @ Sat, 03 Apr 2021 07:50:37: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:50:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:50:37: #1 tags after filtering in treatment: 16619755 INFO @ Sat, 03 Apr 2021 07:50:37: #1 Redundant rate of treatment: 0.22 INFO @ Sat, 03 Apr 2021 07:50:37: #1 finished! INFO @ Sat, 03 Apr 2021 07:50:37: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:50:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:50:39: #2 number of paired peaks: 91 WARNING @ Sat, 03 Apr 2021 07:50:39: Too few paired peaks (91) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 07:50:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6430355/SRX6430355.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430355/SRX6430355.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430355/SRX6430355.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430355/SRX6430355.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。