Job ID = 12265336 SRX = SRX6430353 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 31213468 spots for SRR9669888/SRR9669888.sra Written 31213468 spots for SRR9669888/SRR9669888.sra fastq に変換しました。 bowtie でマッピング中... Your job 12265580 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:31:49 31213468 reads; of these: 31213468 (100.00%) were paired; of these: 9257054 (29.66%) aligned concordantly 0 times 16113692 (51.62%) aligned concordantly exactly 1 time 5842722 (18.72%) aligned concordantly >1 times ---- 9257054 pairs aligned concordantly 0 times; of these: 3744358 (40.45%) aligned discordantly 1 time ---- 5512696 pairs aligned 0 times concordantly or discordantly; of these: 11025392 mates make up the pairs; of these: 8091403 (73.39%) aligned 0 times 1272890 (11.55%) aligned exactly 1 time 1661099 (15.07%) aligned >1 times 87.04% overall alignment rate Time searching: 00:31:49 Overall time: 00:31:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 3510096 / 25298312 = 0.1387 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:25:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6430353/SRX6430353.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6430353/SRX6430353.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6430353/SRX6430353.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6430353/SRX6430353.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:25:43: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:25:43: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:25:49: 1000000 INFO @ Sat, 03 Apr 2021 07:25:54: 2000000 INFO @ Sat, 03 Apr 2021 07:25:59: 3000000 INFO @ Sat, 03 Apr 2021 07:26:04: 4000000 INFO @ Sat, 03 Apr 2021 07:26:09: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:26:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6430353/SRX6430353.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6430353/SRX6430353.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6430353/SRX6430353.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6430353/SRX6430353.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:26:13: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:26:13: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:26:14: 6000000 INFO @ Sat, 03 Apr 2021 07:26:19: 1000000 INFO @ Sat, 03 Apr 2021 07:26:19: 7000000 INFO @ Sat, 03 Apr 2021 07:26:24: 2000000 INFO @ Sat, 03 Apr 2021 07:26:25: 8000000 INFO @ Sat, 03 Apr 2021 07:26:29: 3000000 INFO @ Sat, 03 Apr 2021 07:26:30: 9000000 INFO @ Sat, 03 Apr 2021 07:26:35: 4000000 INFO @ Sat, 03 Apr 2021 07:26:36: 10000000 INFO @ Sat, 03 Apr 2021 07:26:40: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:26:41: 11000000 INFO @ Sat, 03 Apr 2021 07:26:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6430353/SRX6430353.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6430353/SRX6430353.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6430353/SRX6430353.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6430353/SRX6430353.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:26:43: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:26:43: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:26:46: 6000000 INFO @ Sat, 03 Apr 2021 07:26:47: 12000000 INFO @ Sat, 03 Apr 2021 07:26:49: 1000000 INFO @ Sat, 03 Apr 2021 07:26:52: 7000000 INFO @ Sat, 03 Apr 2021 07:26:53: 13000000 INFO @ Sat, 03 Apr 2021 07:26:56: 2000000 INFO @ Sat, 03 Apr 2021 07:26:58: 8000000 INFO @ Sat, 03 Apr 2021 07:26:59: 14000000 INFO @ Sat, 03 Apr 2021 07:27:02: 3000000 INFO @ Sat, 03 Apr 2021 07:27:04: 9000000 INFO @ Sat, 03 Apr 2021 07:27:05: 15000000 INFO @ Sat, 03 Apr 2021 07:27:09: 4000000 INFO @ Sat, 03 Apr 2021 07:27:10: 10000000 INFO @ Sat, 03 Apr 2021 07:27:11: 16000000 INFO @ Sat, 03 Apr 2021 07:27:15: 5000000 INFO @ Sat, 03 Apr 2021 07:27:16: 11000000 INFO @ Sat, 03 Apr 2021 07:27:17: 17000000 INFO @ Sat, 03 Apr 2021 07:27:21: 6000000 INFO @ Sat, 03 Apr 2021 07:27:22: 12000000 INFO @ Sat, 03 Apr 2021 07:27:23: 18000000 INFO @ Sat, 03 Apr 2021 07:27:28: 13000000 INFO @ Sat, 03 Apr 2021 07:27:28: 7000000 INFO @ Sat, 03 Apr 2021 07:27:29: 19000000 INFO @ Sat, 03 Apr 2021 07:27:34: 14000000 INFO @ Sat, 03 Apr 2021 07:27:34: 8000000 INFO @ Sat, 03 Apr 2021 07:27:35: 20000000 INFO @ Sat, 03 Apr 2021 07:27:39: 15000000 INFO @ Sat, 03 Apr 2021 07:27:41: 21000000 INFO @ Sat, 03 Apr 2021 07:27:41: 9000000 INFO @ Sat, 03 Apr 2021 07:27:46: 16000000 INFO @ Sat, 03 Apr 2021 07:27:47: 22000000 INFO @ Sat, 03 Apr 2021 07:27:48: 10000000 INFO @ Sat, 03 Apr 2021 07:27:52: 17000000 INFO @ Sat, 03 Apr 2021 07:27:53: 23000000 INFO @ Sat, 03 Apr 2021 07:27:55: 11000000 INFO @ Sat, 03 Apr 2021 07:27:58: 18000000 INFO @ Sat, 03 Apr 2021 07:28:00: 24000000 INFO @ Sat, 03 Apr 2021 07:28:02: 12000000 INFO @ Sat, 03 Apr 2021 07:28:04: 19000000 INFO @ Sat, 03 Apr 2021 07:28:06: 25000000 INFO @ Sat, 03 Apr 2021 07:28:08: 13000000 INFO @ Sat, 03 Apr 2021 07:28:10: 20000000 INFO @ Sat, 03 Apr 2021 07:28:12: 26000000 INFO @ Sat, 03 Apr 2021 07:28:15: 14000000 INFO @ Sat, 03 Apr 2021 07:28:17: 21000000 INFO @ Sat, 03 Apr 2021 07:28:18: 27000000 INFO @ Sat, 03 Apr 2021 07:28:22: 15000000 INFO @ Sat, 03 Apr 2021 07:28:23: 22000000 INFO @ Sat, 03 Apr 2021 07:28:24: 28000000 INFO @ Sat, 03 Apr 2021 07:28:28: 16000000 INFO @ Sat, 03 Apr 2021 07:28:29: 23000000 INFO @ Sat, 03 Apr 2021 07:28:31: 29000000 INFO @ Sat, 03 Apr 2021 07:28:35: 24000000 INFO @ Sat, 03 Apr 2021 07:28:35: 17000000 INFO @ Sat, 03 Apr 2021 07:28:37: 30000000 INFO @ Sat, 03 Apr 2021 07:28:41: 25000000 INFO @ Sat, 03 Apr 2021 07:28:42: 18000000 INFO @ Sat, 03 Apr 2021 07:28:44: 31000000 INFO @ Sat, 03 Apr 2021 07:28:47: 26000000 INFO @ Sat, 03 Apr 2021 07:28:49: 19000000 INFO @ Sat, 03 Apr 2021 07:28:50: 32000000 INFO @ Sat, 03 Apr 2021 07:28:54: 27000000 INFO @ Sat, 03 Apr 2021 07:28:56: 20000000 INFO @ Sat, 03 Apr 2021 07:28:57: 33000000 INFO @ Sat, 03 Apr 2021 07:29:00: 28000000 INFO @ Sat, 03 Apr 2021 07:29:03: 34000000 INFO @ Sat, 03 Apr 2021 07:29:03: 21000000 INFO @ Sat, 03 Apr 2021 07:29:06: 29000000 INFO @ Sat, 03 Apr 2021 07:29:09: 35000000 INFO @ Sat, 03 Apr 2021 07:29:10: 22000000 INFO @ Sat, 03 Apr 2021 07:29:13: 30000000 INFO @ Sat, 03 Apr 2021 07:29:16: 36000000 INFO @ Sat, 03 Apr 2021 07:29:18: 23000000 INFO @ Sat, 03 Apr 2021 07:29:19: 31000000 INFO @ Sat, 03 Apr 2021 07:29:22: 37000000 INFO @ Sat, 03 Apr 2021 07:29:25: 24000000 INFO @ Sat, 03 Apr 2021 07:29:25: 32000000 INFO @ Sat, 03 Apr 2021 07:29:29: 38000000 INFO @ Sat, 03 Apr 2021 07:29:32: 33000000 INFO @ Sat, 03 Apr 2021 07:29:33: 25000000 INFO @ Sat, 03 Apr 2021 07:29:35: 39000000 INFO @ Sat, 03 Apr 2021 07:29:38: 34000000 INFO @ Sat, 03 Apr 2021 07:29:40: 26000000 INFO @ Sat, 03 Apr 2021 07:29:42: 40000000 INFO @ Sat, 03 Apr 2021 07:29:44: 35000000 INFO @ Sat, 03 Apr 2021 07:29:48: 27000000 INFO @ Sat, 03 Apr 2021 07:29:48: 41000000 INFO @ Sat, 03 Apr 2021 07:29:50: 36000000 INFO @ Sat, 03 Apr 2021 07:29:55: 42000000 INFO @ Sat, 03 Apr 2021 07:29:55: 28000000 INFO @ Sat, 03 Apr 2021 07:29:57: 37000000 INFO @ Sat, 03 Apr 2021 07:30:01: 43000000 INFO @ Sat, 03 Apr 2021 07:30:03: 29000000 INFO @ Sat, 03 Apr 2021 07:30:03: 38000000 INFO @ Sat, 03 Apr 2021 07:30:07: 44000000 INFO @ Sat, 03 Apr 2021 07:30:09: 39000000 INFO @ Sat, 03 Apr 2021 07:30:10: 30000000 INFO @ Sat, 03 Apr 2021 07:30:14: 45000000 INFO @ Sat, 03 Apr 2021 07:30:15: 40000000 INFO @ Sat, 03 Apr 2021 07:30:18: 31000000 INFO @ Sat, 03 Apr 2021 07:30:20: 46000000 INFO @ Sat, 03 Apr 2021 07:30:22: 41000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 07:30:25: 32000000 INFO @ Sat, 03 Apr 2021 07:30:26: 47000000 INFO @ Sat, 03 Apr 2021 07:30:28: 42000000 INFO @ Sat, 03 Apr 2021 07:30:29: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:30:29: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:30:29: #1 total tags in treatment: 18714104 INFO @ Sat, 03 Apr 2021 07:30:29: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:30:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:30:29: #1 tags after filtering in treatment: 15156322 INFO @ Sat, 03 Apr 2021 07:30:29: #1 Redundant rate of treatment: 0.19 INFO @ Sat, 03 Apr 2021 07:30:29: #1 finished! INFO @ Sat, 03 Apr 2021 07:30:29: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:30:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:30:30: #2 number of paired peaks: 87 WARNING @ Sat, 03 Apr 2021 07:30:30: Too few paired peaks (87) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 07:30:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6430353/SRX6430353.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430353/SRX6430353.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430353/SRX6430353.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430353/SRX6430353.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:30:32: 33000000 INFO @ Sat, 03 Apr 2021 07:30:34: 43000000 INFO @ Sat, 03 Apr 2021 07:30:39: 34000000 INFO @ Sat, 03 Apr 2021 07:30:40: 44000000 INFO @ Sat, 03 Apr 2021 07:30:45: 35000000 INFO @ Sat, 03 Apr 2021 07:30:46: 45000000 INFO @ Sat, 03 Apr 2021 07:30:52: 46000000 INFO @ Sat, 03 Apr 2021 07:30:52: 36000000 INFO @ Sat, 03 Apr 2021 07:30:58: 47000000 INFO @ Sat, 03 Apr 2021 07:30:59: 37000000 INFO @ Sat, 03 Apr 2021 07:31:00: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:31:00: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:31:00: #1 total tags in treatment: 18714104 INFO @ Sat, 03 Apr 2021 07:31:00: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:31:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:31:01: #1 tags after filtering in treatment: 15156322 INFO @ Sat, 03 Apr 2021 07:31:01: #1 Redundant rate of treatment: 0.19 INFO @ Sat, 03 Apr 2021 07:31:01: #1 finished! INFO @ Sat, 03 Apr 2021 07:31:01: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:31:01: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:31:02: #2 number of paired peaks: 87 WARNING @ Sat, 03 Apr 2021 07:31:02: Too few paired peaks (87) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 07:31:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6430353/SRX6430353.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430353/SRX6430353.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430353/SRX6430353.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430353/SRX6430353.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:31:05: 38000000 INFO @ Sat, 03 Apr 2021 07:31:11: 39000000 INFO @ Sat, 03 Apr 2021 07:31:17: 40000000 INFO @ Sat, 03 Apr 2021 07:31:23: 41000000 INFO @ Sat, 03 Apr 2021 07:31:30: 42000000 INFO @ Sat, 03 Apr 2021 07:31:36: 43000000 INFO @ Sat, 03 Apr 2021 07:31:42: 44000000 BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 07:31:48: 45000000 INFO @ Sat, 03 Apr 2021 07:31:55: 46000000 INFO @ Sat, 03 Apr 2021 07:32:01: 47000000 INFO @ Sat, 03 Apr 2021 07:32:03: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:32:03: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:32:03: #1 total tags in treatment: 18714104 INFO @ Sat, 03 Apr 2021 07:32:03: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:32:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:32:04: #1 tags after filtering in treatment: 15156322 INFO @ Sat, 03 Apr 2021 07:32:04: #1 Redundant rate of treatment: 0.19 INFO @ Sat, 03 Apr 2021 07:32:04: #1 finished! INFO @ Sat, 03 Apr 2021 07:32:04: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:32:04: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:32:05: #2 number of paired peaks: 87 WARNING @ Sat, 03 Apr 2021 07:32:05: Too few paired peaks (87) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 07:32:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6430353/SRX6430353.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430353/SRX6430353.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430353/SRX6430353.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430353/SRX6430353.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling