Job ID = 12265331 SRX = SRX6430351 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 34227371 spots for SRR9669889/SRR9669889.sra Written 34227371 spots for SRR9669889/SRR9669889.sra fastq に変換しました。 bowtie でマッピング中... Your job 12265601 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:36:57 34227371 reads; of these: 34227371 (100.00%) were paired; of these: 11376924 (33.24%) aligned concordantly 0 times 17360888 (50.72%) aligned concordantly exactly 1 time 5489559 (16.04%) aligned concordantly >1 times ---- 11376924 pairs aligned concordantly 0 times; of these: 4446457 (39.08%) aligned discordantly 1 time ---- 6930467 pairs aligned 0 times concordantly or discordantly; of these: 13860934 mates make up the pairs; of these: 10300623 (74.31%) aligned 0 times 1576198 (11.37%) aligned exactly 1 time 1984113 (14.31%) aligned >1 times 84.95% overall alignment rate Time searching: 00:36:57 Overall time: 00:36:57 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 3271408 / 26818574 = 0.1220 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:32:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6430351/SRX6430351.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6430351/SRX6430351.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6430351/SRX6430351.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6430351/SRX6430351.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:32:11: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:32:11: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:32:16: 1000000 INFO @ Sat, 03 Apr 2021 07:32:21: 2000000 INFO @ Sat, 03 Apr 2021 07:32:26: 3000000 INFO @ Sat, 03 Apr 2021 07:32:31: 4000000 INFO @ Sat, 03 Apr 2021 07:32:36: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:32:41: 6000000 INFO @ Sat, 03 Apr 2021 07:32:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6430351/SRX6430351.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6430351/SRX6430351.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6430351/SRX6430351.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6430351/SRX6430351.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:32:41: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:32:41: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:32:46: 7000000 INFO @ Sat, 03 Apr 2021 07:32:46: 1000000 INFO @ Sat, 03 Apr 2021 07:32:51: 8000000 INFO @ Sat, 03 Apr 2021 07:32:52: 2000000 INFO @ Sat, 03 Apr 2021 07:32:57: 9000000 INFO @ Sat, 03 Apr 2021 07:32:57: 3000000 INFO @ Sat, 03 Apr 2021 07:33:02: 10000000 INFO @ Sat, 03 Apr 2021 07:33:03: 4000000 INFO @ Sat, 03 Apr 2021 07:33:07: 11000000 INFO @ Sat, 03 Apr 2021 07:33:08: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:33:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6430351/SRX6430351.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6430351/SRX6430351.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6430351/SRX6430351.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6430351/SRX6430351.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:33:11: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:33:11: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:33:13: 12000000 INFO @ Sat, 03 Apr 2021 07:33:14: 6000000 INFO @ Sat, 03 Apr 2021 07:33:16: 1000000 INFO @ Sat, 03 Apr 2021 07:33:18: 13000000 INFO @ Sat, 03 Apr 2021 07:33:19: 7000000 INFO @ Sat, 03 Apr 2021 07:33:22: 2000000 INFO @ Sat, 03 Apr 2021 07:33:23: 14000000 INFO @ Sat, 03 Apr 2021 07:33:25: 8000000 INFO @ Sat, 03 Apr 2021 07:33:27: 3000000 INFO @ Sat, 03 Apr 2021 07:33:29: 15000000 INFO @ Sat, 03 Apr 2021 07:33:30: 9000000 INFO @ Sat, 03 Apr 2021 07:33:33: 4000000 INFO @ Sat, 03 Apr 2021 07:33:34: 16000000 INFO @ Sat, 03 Apr 2021 07:33:36: 10000000 INFO @ Sat, 03 Apr 2021 07:33:38: 5000000 INFO @ Sat, 03 Apr 2021 07:33:39: 17000000 INFO @ Sat, 03 Apr 2021 07:33:41: 11000000 INFO @ Sat, 03 Apr 2021 07:33:44: 6000000 INFO @ Sat, 03 Apr 2021 07:33:45: 18000000 INFO @ Sat, 03 Apr 2021 07:33:46: 12000000 INFO @ Sat, 03 Apr 2021 07:33:49: 7000000 INFO @ Sat, 03 Apr 2021 07:33:50: 19000000 INFO @ Sat, 03 Apr 2021 07:33:52: 13000000 INFO @ Sat, 03 Apr 2021 07:33:54: 8000000 INFO @ Sat, 03 Apr 2021 07:33:55: 20000000 INFO @ Sat, 03 Apr 2021 07:33:57: 14000000 INFO @ Sat, 03 Apr 2021 07:34:00: 9000000 INFO @ Sat, 03 Apr 2021 07:34:01: 21000000 INFO @ Sat, 03 Apr 2021 07:34:02: 15000000 INFO @ Sat, 03 Apr 2021 07:34:05: 10000000 INFO @ Sat, 03 Apr 2021 07:34:06: 22000000 INFO @ Sat, 03 Apr 2021 07:34:08: 16000000 INFO @ Sat, 03 Apr 2021 07:34:11: 11000000 INFO @ Sat, 03 Apr 2021 07:34:11: 23000000 INFO @ Sat, 03 Apr 2021 07:34:13: 17000000 INFO @ Sat, 03 Apr 2021 07:34:16: 12000000 INFO @ Sat, 03 Apr 2021 07:34:17: 24000000 INFO @ Sat, 03 Apr 2021 07:34:18: 18000000 INFO @ Sat, 03 Apr 2021 07:34:21: 13000000 INFO @ Sat, 03 Apr 2021 07:34:22: 25000000 INFO @ Sat, 03 Apr 2021 07:34:24: 19000000 INFO @ Sat, 03 Apr 2021 07:34:26: 14000000 INFO @ Sat, 03 Apr 2021 07:34:27: 26000000 INFO @ Sat, 03 Apr 2021 07:34:29: 20000000 INFO @ Sat, 03 Apr 2021 07:34:32: 15000000 INFO @ Sat, 03 Apr 2021 07:34:32: 27000000 INFO @ Sat, 03 Apr 2021 07:34:34: 21000000 INFO @ Sat, 03 Apr 2021 07:34:37: 16000000 INFO @ Sat, 03 Apr 2021 07:34:38: 28000000 INFO @ Sat, 03 Apr 2021 07:34:39: 22000000 INFO @ Sat, 03 Apr 2021 07:34:42: 17000000 INFO @ Sat, 03 Apr 2021 07:34:43: 29000000 INFO @ Sat, 03 Apr 2021 07:34:45: 23000000 INFO @ Sat, 03 Apr 2021 07:34:47: 18000000 INFO @ Sat, 03 Apr 2021 07:34:48: 30000000 INFO @ Sat, 03 Apr 2021 07:34:50: 24000000 INFO @ Sat, 03 Apr 2021 07:34:53: 19000000 INFO @ Sat, 03 Apr 2021 07:34:54: 31000000 INFO @ Sat, 03 Apr 2021 07:34:55: 25000000 INFO @ Sat, 03 Apr 2021 07:34:58: 20000000 INFO @ Sat, 03 Apr 2021 07:34:59: 32000000 INFO @ Sat, 03 Apr 2021 07:35:01: 26000000 INFO @ Sat, 03 Apr 2021 07:35:03: 21000000 INFO @ Sat, 03 Apr 2021 07:35:04: 33000000 INFO @ Sat, 03 Apr 2021 07:35:06: 27000000 INFO @ Sat, 03 Apr 2021 07:35:09: 22000000 INFO @ Sat, 03 Apr 2021 07:35:09: 34000000 INFO @ Sat, 03 Apr 2021 07:35:11: 28000000 INFO @ Sat, 03 Apr 2021 07:35:14: 23000000 INFO @ Sat, 03 Apr 2021 07:35:15: 35000000 INFO @ Sat, 03 Apr 2021 07:35:16: 29000000 INFO @ Sat, 03 Apr 2021 07:35:19: 24000000 INFO @ Sat, 03 Apr 2021 07:35:20: 36000000 INFO @ Sat, 03 Apr 2021 07:35:22: 30000000 INFO @ Sat, 03 Apr 2021 07:35:24: 25000000 INFO @ Sat, 03 Apr 2021 07:35:25: 37000000 INFO @ Sat, 03 Apr 2021 07:35:27: 31000000 INFO @ Sat, 03 Apr 2021 07:35:29: 26000000 INFO @ Sat, 03 Apr 2021 07:35:30: 38000000 INFO @ Sat, 03 Apr 2021 07:35:32: 32000000 INFO @ Sat, 03 Apr 2021 07:35:35: 27000000 INFO @ Sat, 03 Apr 2021 07:35:35: 39000000 INFO @ Sat, 03 Apr 2021 07:35:37: 33000000 INFO @ Sat, 03 Apr 2021 07:35:40: 28000000 INFO @ Sat, 03 Apr 2021 07:35:40: 40000000 INFO @ Sat, 03 Apr 2021 07:35:43: 34000000 INFO @ Sat, 03 Apr 2021 07:35:45: 29000000 INFO @ Sat, 03 Apr 2021 07:35:46: 41000000 INFO @ Sat, 03 Apr 2021 07:35:48: 35000000 INFO @ Sat, 03 Apr 2021 07:35:50: 30000000 INFO @ Sat, 03 Apr 2021 07:35:51: 42000000 INFO @ Sat, 03 Apr 2021 07:35:53: 36000000 INFO @ Sat, 03 Apr 2021 07:35:55: 31000000 INFO @ Sat, 03 Apr 2021 07:35:56: 43000000 INFO @ Sat, 03 Apr 2021 07:35:58: 37000000 INFO @ Sat, 03 Apr 2021 07:36:00: 32000000 INFO @ Sat, 03 Apr 2021 07:36:01: 44000000 INFO @ Sat, 03 Apr 2021 07:36:03: 38000000 INFO @ Sat, 03 Apr 2021 07:36:05: 33000000 INFO @ Sat, 03 Apr 2021 07:36:06: 45000000 INFO @ Sat, 03 Apr 2021 07:36:08: 39000000 INFO @ Sat, 03 Apr 2021 07:36:10: 34000000 INFO @ Sat, 03 Apr 2021 07:36:11: 46000000 INFO @ Sat, 03 Apr 2021 07:36:13: 40000000 INFO @ Sat, 03 Apr 2021 07:36:15: 35000000 INFO @ Sat, 03 Apr 2021 07:36:16: 47000000 INFO @ Sat, 03 Apr 2021 07:36:18: 41000000 INFO @ Sat, 03 Apr 2021 07:36:20: 36000000 INFO @ Sat, 03 Apr 2021 07:36:21: 48000000 INFO @ Sat, 03 Apr 2021 07:36:23: 42000000 INFO @ Sat, 03 Apr 2021 07:36:25: 37000000 INFO @ Sat, 03 Apr 2021 07:36:26: 49000000 INFO @ Sat, 03 Apr 2021 07:36:28: 43000000 INFO @ Sat, 03 Apr 2021 07:36:31: 50000000 INFO @ Sat, 03 Apr 2021 07:36:31: 38000000 INFO @ Sat, 03 Apr 2021 07:36:34: 44000000 INFO @ Sat, 03 Apr 2021 07:36:36: 51000000 INFO @ Sat, 03 Apr 2021 07:36:36: 39000000 INFO @ Sat, 03 Apr 2021 07:36:39: 45000000 INFO @ Sat, 03 Apr 2021 07:36:39: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:36:39: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:36:39: #1 total tags in treatment: 19990290 INFO @ Sat, 03 Apr 2021 07:36:39: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:36:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:36:40: #1 tags after filtering in treatment: 16270328 INFO @ Sat, 03 Apr 2021 07:36:40: #1 Redundant rate of treatment: 0.19 INFO @ Sat, 03 Apr 2021 07:36:40: #1 finished! INFO @ Sat, 03 Apr 2021 07:36:40: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:36:40: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:36:41: #2 number of paired peaks: 88 WARNING @ Sat, 03 Apr 2021 07:36:41: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 07:36:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6430351/SRX6430351.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430351/SRX6430351.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430351/SRX6430351.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430351/SRX6430351.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:36:41: 40000000 INFO @ Sat, 03 Apr 2021 07:36:44: 46000000 INFO @ Sat, 03 Apr 2021 07:36:47: 41000000 INFO @ Sat, 03 Apr 2021 07:36:49: 47000000 INFO @ Sat, 03 Apr 2021 07:36:52: 42000000 INFO @ Sat, 03 Apr 2021 07:36:55: 48000000 INFO @ Sat, 03 Apr 2021 07:36:57: 43000000 INFO @ Sat, 03 Apr 2021 07:37:00: 49000000 INFO @ Sat, 03 Apr 2021 07:37:02: 44000000 INFO @ Sat, 03 Apr 2021 07:37:05: 50000000 INFO @ Sat, 03 Apr 2021 07:37:07: 45000000 INFO @ Sat, 03 Apr 2021 07:37:10: 51000000 INFO @ Sat, 03 Apr 2021 07:37:12: 46000000 INFO @ Sat, 03 Apr 2021 07:37:13: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:37:13: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:37:13: #1 total tags in treatment: 19990290 INFO @ Sat, 03 Apr 2021 07:37:13: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:37:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:37:13: #1 tags after filtering in treatment: 16270328 INFO @ Sat, 03 Apr 2021 07:37:13: #1 Redundant rate of treatment: 0.19 INFO @ Sat, 03 Apr 2021 07:37:13: #1 finished! INFO @ Sat, 03 Apr 2021 07:37:13: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:37:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:37:15: #2 number of paired peaks: 88 WARNING @ Sat, 03 Apr 2021 07:37:15: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 07:37:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6430351/SRX6430351.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430351/SRX6430351.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430351/SRX6430351.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430351/SRX6430351.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:37:17: 47000000 INFO @ Sat, 03 Apr 2021 07:37:22: 48000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 07:37:27: 49000000 INFO @ Sat, 03 Apr 2021 07:37:32: 50000000 INFO @ Sat, 03 Apr 2021 07:37:37: 51000000 INFO @ Sat, 03 Apr 2021 07:37:40: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:37:40: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:37:40: #1 total tags in treatment: 19990290 INFO @ Sat, 03 Apr 2021 07:37:40: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:37:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:37:41: #1 tags after filtering in treatment: 16270328 INFO @ Sat, 03 Apr 2021 07:37:41: #1 Redundant rate of treatment: 0.19 INFO @ Sat, 03 Apr 2021 07:37:41: #1 finished! INFO @ Sat, 03 Apr 2021 07:37:41: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:37:41: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:37:42: #2 number of paired peaks: 88 WARNING @ Sat, 03 Apr 2021 07:37:42: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 07:37:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6430351/SRX6430351.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430351/SRX6430351.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430351/SRX6430351.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430351/SRX6430351.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。