Job ID = 12265329 SRX = SRX6430350 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 32387214 spots for SRR9669890/SRR9669890.sra Written 32387214 spots for SRR9669890/SRR9669890.sra fastq に変換しました。 bowtie でマッピング中... Your job 12265589 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:33:49 32387214 reads; of these: 32387214 (100.00%) were paired; of these: 10077138 (31.11%) aligned concordantly 0 times 17260631 (53.29%) aligned concordantly exactly 1 time 5049445 (15.59%) aligned concordantly >1 times ---- 10077138 pairs aligned concordantly 0 times; of these: 4074030 (40.43%) aligned discordantly 1 time ---- 6003108 pairs aligned 0 times concordantly or discordantly; of these: 12006216 mates make up the pairs; of these: 8990352 (74.88%) aligned 0 times 1402122 (11.68%) aligned exactly 1 time 1613742 (13.44%) aligned >1 times 86.12% overall alignment rate Time searching: 00:33:49 Overall time: 00:33:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 2705769 / 25951182 = 0.1043 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:28:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6430350/SRX6430350.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6430350/SRX6430350.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6430350/SRX6430350.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6430350/SRX6430350.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:28:34: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:28:34: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:28:39: 1000000 INFO @ Sat, 03 Apr 2021 07:28:44: 2000000 INFO @ Sat, 03 Apr 2021 07:28:49: 3000000 INFO @ Sat, 03 Apr 2021 07:28:55: 4000000 INFO @ Sat, 03 Apr 2021 07:29:00: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:29:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6430350/SRX6430350.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6430350/SRX6430350.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6430350/SRX6430350.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6430350/SRX6430350.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:29:04: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:29:04: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:29:05: 6000000 INFO @ Sat, 03 Apr 2021 07:29:10: 1000000 INFO @ Sat, 03 Apr 2021 07:29:11: 7000000 INFO @ Sat, 03 Apr 2021 07:29:16: 2000000 INFO @ Sat, 03 Apr 2021 07:29:16: 8000000 INFO @ Sat, 03 Apr 2021 07:29:21: 3000000 INFO @ Sat, 03 Apr 2021 07:29:22: 9000000 INFO @ Sat, 03 Apr 2021 07:29:27: 4000000 INFO @ Sat, 03 Apr 2021 07:29:28: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:29:33: 5000000 INFO @ Sat, 03 Apr 2021 07:29:33: 11000000 INFO @ Sat, 03 Apr 2021 07:29:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6430350/SRX6430350.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6430350/SRX6430350.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6430350/SRX6430350.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6430350/SRX6430350.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:29:34: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:29:34: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:29:38: 6000000 INFO @ Sat, 03 Apr 2021 07:29:39: 12000000 INFO @ Sat, 03 Apr 2021 07:29:40: 1000000 INFO @ Sat, 03 Apr 2021 07:29:44: 7000000 INFO @ Sat, 03 Apr 2021 07:29:45: 13000000 INFO @ Sat, 03 Apr 2021 07:29:46: 2000000 INFO @ Sat, 03 Apr 2021 07:29:50: 8000000 INFO @ Sat, 03 Apr 2021 07:29:50: 14000000 INFO @ Sat, 03 Apr 2021 07:29:52: 3000000 INFO @ Sat, 03 Apr 2021 07:29:56: 9000000 INFO @ Sat, 03 Apr 2021 07:29:56: 15000000 INFO @ Sat, 03 Apr 2021 07:29:58: 4000000 INFO @ Sat, 03 Apr 2021 07:30:01: 10000000 INFO @ Sat, 03 Apr 2021 07:30:02: 16000000 INFO @ Sat, 03 Apr 2021 07:30:04: 5000000 INFO @ Sat, 03 Apr 2021 07:30:07: 11000000 INFO @ Sat, 03 Apr 2021 07:30:08: 17000000 INFO @ Sat, 03 Apr 2021 07:30:09: 6000000 INFO @ Sat, 03 Apr 2021 07:30:13: 12000000 INFO @ Sat, 03 Apr 2021 07:30:13: 18000000 INFO @ Sat, 03 Apr 2021 07:30:15: 7000000 INFO @ Sat, 03 Apr 2021 07:30:19: 13000000 INFO @ Sat, 03 Apr 2021 07:30:19: 19000000 INFO @ Sat, 03 Apr 2021 07:30:21: 8000000 INFO @ Sat, 03 Apr 2021 07:30:24: 14000000 INFO @ Sat, 03 Apr 2021 07:30:25: 20000000 INFO @ Sat, 03 Apr 2021 07:30:27: 9000000 INFO @ Sat, 03 Apr 2021 07:30:30: 15000000 INFO @ Sat, 03 Apr 2021 07:30:30: 21000000 INFO @ Sat, 03 Apr 2021 07:30:32: 10000000 INFO @ Sat, 03 Apr 2021 07:30:36: 16000000 INFO @ Sat, 03 Apr 2021 07:30:36: 22000000 INFO @ Sat, 03 Apr 2021 07:30:38: 11000000 INFO @ Sat, 03 Apr 2021 07:30:42: 17000000 INFO @ Sat, 03 Apr 2021 07:30:42: 23000000 INFO @ Sat, 03 Apr 2021 07:30:44: 12000000 INFO @ Sat, 03 Apr 2021 07:30:47: 24000000 INFO @ Sat, 03 Apr 2021 07:30:48: 18000000 INFO @ Sat, 03 Apr 2021 07:30:49: 13000000 INFO @ Sat, 03 Apr 2021 07:30:53: 25000000 INFO @ Sat, 03 Apr 2021 07:30:53: 19000000 INFO @ Sat, 03 Apr 2021 07:30:55: 14000000 INFO @ Sat, 03 Apr 2021 07:30:59: 26000000 INFO @ Sat, 03 Apr 2021 07:30:59: 20000000 INFO @ Sat, 03 Apr 2021 07:31:01: 15000000 INFO @ Sat, 03 Apr 2021 07:31:04: 27000000 INFO @ Sat, 03 Apr 2021 07:31:05: 21000000 INFO @ Sat, 03 Apr 2021 07:31:07: 16000000 INFO @ Sat, 03 Apr 2021 07:31:10: 28000000 INFO @ Sat, 03 Apr 2021 07:31:10: 22000000 INFO @ Sat, 03 Apr 2021 07:31:12: 17000000 INFO @ Sat, 03 Apr 2021 07:31:16: 29000000 INFO @ Sat, 03 Apr 2021 07:31:16: 23000000 INFO @ Sat, 03 Apr 2021 07:31:18: 18000000 INFO @ Sat, 03 Apr 2021 07:31:21: 30000000 INFO @ Sat, 03 Apr 2021 07:31:22: 24000000 INFO @ Sat, 03 Apr 2021 07:31:24: 19000000 INFO @ Sat, 03 Apr 2021 07:31:27: 31000000 INFO @ Sat, 03 Apr 2021 07:31:28: 25000000 INFO @ Sat, 03 Apr 2021 07:31:29: 20000000 INFO @ Sat, 03 Apr 2021 07:31:33: 32000000 INFO @ Sat, 03 Apr 2021 07:31:33: 26000000 INFO @ Sat, 03 Apr 2021 07:31:35: 21000000 INFO @ Sat, 03 Apr 2021 07:31:39: 33000000 INFO @ Sat, 03 Apr 2021 07:31:39: 27000000 INFO @ Sat, 03 Apr 2021 07:31:41: 22000000 INFO @ Sat, 03 Apr 2021 07:31:44: 34000000 INFO @ Sat, 03 Apr 2021 07:31:45: 28000000 INFO @ Sat, 03 Apr 2021 07:31:47: 23000000 INFO @ Sat, 03 Apr 2021 07:31:50: 35000000 INFO @ Sat, 03 Apr 2021 07:31:51: 29000000 INFO @ Sat, 03 Apr 2021 07:31:52: 24000000 INFO @ Sat, 03 Apr 2021 07:31:56: 36000000 INFO @ Sat, 03 Apr 2021 07:31:56: 30000000 INFO @ Sat, 03 Apr 2021 07:31:58: 25000000 INFO @ Sat, 03 Apr 2021 07:32:01: 37000000 INFO @ Sat, 03 Apr 2021 07:32:02: 31000000 INFO @ Sat, 03 Apr 2021 07:32:04: 26000000 INFO @ Sat, 03 Apr 2021 07:32:07: 38000000 INFO @ Sat, 03 Apr 2021 07:32:08: 32000000 INFO @ Sat, 03 Apr 2021 07:32:09: 27000000 INFO @ Sat, 03 Apr 2021 07:32:12: 39000000 INFO @ Sat, 03 Apr 2021 07:32:13: 33000000 INFO @ Sat, 03 Apr 2021 07:32:15: 28000000 INFO @ Sat, 03 Apr 2021 07:32:18: 40000000 INFO @ Sat, 03 Apr 2021 07:32:19: 34000000 INFO @ Sat, 03 Apr 2021 07:32:21: 29000000 INFO @ Sat, 03 Apr 2021 07:32:24: 41000000 INFO @ Sat, 03 Apr 2021 07:32:25: 35000000 INFO @ Sat, 03 Apr 2021 07:32:26: 30000000 INFO @ Sat, 03 Apr 2021 07:32:29: 42000000 INFO @ Sat, 03 Apr 2021 07:32:31: 36000000 INFO @ Sat, 03 Apr 2021 07:32:32: 31000000 INFO @ Sat, 03 Apr 2021 07:32:35: 43000000 INFO @ Sat, 03 Apr 2021 07:32:36: 37000000 INFO @ Sat, 03 Apr 2021 07:32:38: 32000000 INFO @ Sat, 03 Apr 2021 07:32:41: 44000000 INFO @ Sat, 03 Apr 2021 07:32:42: 38000000 INFO @ Sat, 03 Apr 2021 07:32:44: 33000000 INFO @ Sat, 03 Apr 2021 07:32:46: 45000000 INFO @ Sat, 03 Apr 2021 07:32:47: 39000000 INFO @ Sat, 03 Apr 2021 07:32:49: 34000000 INFO @ Sat, 03 Apr 2021 07:32:52: 46000000 INFO @ Sat, 03 Apr 2021 07:32:53: 40000000 INFO @ Sat, 03 Apr 2021 07:32:55: 35000000 INFO @ Sat, 03 Apr 2021 07:32:58: 47000000 INFO @ Sat, 03 Apr 2021 07:32:59: 41000000 INFO @ Sat, 03 Apr 2021 07:33:01: 36000000 INFO @ Sat, 03 Apr 2021 07:33:04: 48000000 INFO @ Sat, 03 Apr 2021 07:33:05: 42000000 INFO @ Sat, 03 Apr 2021 07:33:07: 37000000 INFO @ Sat, 03 Apr 2021 07:33:09: 49000000 INFO @ Sat, 03 Apr 2021 07:33:10: 43000000 INFO @ Sat, 03 Apr 2021 07:33:12: 38000000 INFO @ Sat, 03 Apr 2021 07:33:15: 50000000 INFO @ Sat, 03 Apr 2021 07:33:16: 44000000 INFO @ Sat, 03 Apr 2021 07:33:18: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:33:18: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:33:18: #1 total tags in treatment: 19891240 INFO @ Sat, 03 Apr 2021 07:33:18: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:33:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:33:18: #1 tags after filtering in treatment: 16250070 INFO @ Sat, 03 Apr 2021 07:33:18: #1 Redundant rate of treatment: 0.18 INFO @ Sat, 03 Apr 2021 07:33:18: #1 finished! INFO @ Sat, 03 Apr 2021 07:33:18: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:33:18: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:33:18: 39000000 INFO @ Sat, 03 Apr 2021 07:33:19: #2 number of paired peaks: 90 WARNING @ Sat, 03 Apr 2021 07:33:19: Too few paired peaks (90) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 07:33:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6430350/SRX6430350.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430350/SRX6430350.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430350/SRX6430350.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430350/SRX6430350.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:33:22: 45000000 INFO @ Sat, 03 Apr 2021 07:33:24: 40000000 INFO @ Sat, 03 Apr 2021 07:33:27: 46000000 INFO @ Sat, 03 Apr 2021 07:33:29: 41000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 07:33:33: 47000000 INFO @ Sat, 03 Apr 2021 07:33:35: 42000000 INFO @ Sat, 03 Apr 2021 07:33:39: 48000000 INFO @ Sat, 03 Apr 2021 07:33:41: 43000000 INFO @ Sat, 03 Apr 2021 07:33:45: 49000000 INFO @ Sat, 03 Apr 2021 07:33:47: 44000000 INFO @ Sat, 03 Apr 2021 07:33:51: 50000000 INFO @ Sat, 03 Apr 2021 07:33:53: 45000000 INFO @ Sat, 03 Apr 2021 07:33:53: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:33:53: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:33:53: #1 total tags in treatment: 19891240 INFO @ Sat, 03 Apr 2021 07:33:53: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:33:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:33:54: #1 tags after filtering in treatment: 16250070 INFO @ Sat, 03 Apr 2021 07:33:54: #1 Redundant rate of treatment: 0.18 INFO @ Sat, 03 Apr 2021 07:33:54: #1 finished! INFO @ Sat, 03 Apr 2021 07:33:54: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:33:54: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:33:55: #2 number of paired peaks: 90 WARNING @ Sat, 03 Apr 2021 07:33:55: Too few paired peaks (90) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 07:33:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6430350/SRX6430350.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430350/SRX6430350.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430350/SRX6430350.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430350/SRX6430350.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:33:58: 46000000 INFO @ Sat, 03 Apr 2021 07:34:04: 47000000 INFO @ Sat, 03 Apr 2021 07:34:09: 48000000 INFO @ Sat, 03 Apr 2021 07:34:14: 49000000 INFO @ Sat, 03 Apr 2021 07:34:20: 50000000 INFO @ Sat, 03 Apr 2021 07:34:22: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:34:22: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:34:22: #1 total tags in treatment: 19891240 INFO @ Sat, 03 Apr 2021 07:34:22: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:34:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:34:22: #1 tags after filtering in treatment: 16250070 INFO @ Sat, 03 Apr 2021 07:34:22: #1 Redundant rate of treatment: 0.18 INFO @ Sat, 03 Apr 2021 07:34:22: #1 finished! INFO @ Sat, 03 Apr 2021 07:34:22: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:34:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:34:23: #2 number of paired peaks: 90 WARNING @ Sat, 03 Apr 2021 07:34:23: Too few paired peaks (90) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 03 Apr 2021 07:34:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6430350/SRX6430350.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430350/SRX6430350.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430350/SRX6430350.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6430350/SRX6430350.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。