Job ID = 6528418 SRX = SRX6386732 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:55:43 prefetch.2.10.7: 1) Downloading 'SRR9624464'... 2020-06-29T14:55:43 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:57:15 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:57:16 prefetch.2.10.7: 'SRR9624464' is valid 2020-06-29T14:57:16 prefetch.2.10.7: 1) 'SRR9624464' was downloaded successfully Read 16943864 spots for SRR9624464/SRR9624464.sra Written 16943864 spots for SRR9624464/SRR9624464.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:32 16943864 reads; of these: 16943864 (100.00%) were unpaired; of these: 1363596 (8.05%) aligned 0 times 10942419 (64.58%) aligned exactly 1 time 4637849 (27.37%) aligned >1 times 91.95% overall alignment rate Time searching: 00:05:33 Overall time: 00:05:33 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3567264 / 15580268 = 0.2290 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:11:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6386732/SRX6386732.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6386732/SRX6386732.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6386732/SRX6386732.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6386732/SRX6386732.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:11:12: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:11:12: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:11:17: 1000000 INFO @ Tue, 30 Jun 2020 00:11:22: 2000000 INFO @ Tue, 30 Jun 2020 00:11:27: 3000000 INFO @ Tue, 30 Jun 2020 00:11:32: 4000000 INFO @ Tue, 30 Jun 2020 00:11:37: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:11:42: 6000000 INFO @ Tue, 30 Jun 2020 00:11:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6386732/SRX6386732.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6386732/SRX6386732.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6386732/SRX6386732.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6386732/SRX6386732.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:11:42: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:11:42: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:11:46: 7000000 INFO @ Tue, 30 Jun 2020 00:11:47: 1000000 INFO @ Tue, 30 Jun 2020 00:11:51: 8000000 INFO @ Tue, 30 Jun 2020 00:11:52: 2000000 INFO @ Tue, 30 Jun 2020 00:11:56: 9000000 INFO @ Tue, 30 Jun 2020 00:11:56: 3000000 INFO @ Tue, 30 Jun 2020 00:12:01: 10000000 INFO @ Tue, 30 Jun 2020 00:12:01: 4000000 INFO @ Tue, 30 Jun 2020 00:12:06: 5000000 INFO @ Tue, 30 Jun 2020 00:12:06: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:12:10: 6000000 INFO @ Tue, 30 Jun 2020 00:12:10: 12000000 INFO @ Tue, 30 Jun 2020 00:12:11: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:12:11: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:12:11: #1 total tags in treatment: 12013004 INFO @ Tue, 30 Jun 2020 00:12:11: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:12:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:12:11: #1 tags after filtering in treatment: 12013004 INFO @ Tue, 30 Jun 2020 00:12:11: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:12:11: #1 finished! INFO @ Tue, 30 Jun 2020 00:12:11: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:12:11: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:12:12: #2 number of paired peaks: 84 WARNING @ Tue, 30 Jun 2020 00:12:12: Too few paired peaks (84) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:12:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6386732/SRX6386732.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6386732/SRX6386732.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6386732/SRX6386732.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6386732/SRX6386732.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:12:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX6386732/SRX6386732.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX6386732/SRX6386732.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX6386732/SRX6386732.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX6386732/SRX6386732.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:12:12: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:12:12: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:12:15: 7000000 INFO @ Tue, 30 Jun 2020 00:12:17: 1000000 INFO @ Tue, 30 Jun 2020 00:12:20: 8000000 INFO @ Tue, 30 Jun 2020 00:12:21: 2000000 INFO @ Tue, 30 Jun 2020 00:12:24: 9000000 INFO @ Tue, 30 Jun 2020 00:12:26: 3000000 INFO @ Tue, 30 Jun 2020 00:12:29: 10000000 INFO @ Tue, 30 Jun 2020 00:12:31: 4000000 INFO @ Tue, 30 Jun 2020 00:12:34: 11000000 INFO @ Tue, 30 Jun 2020 00:12:36: 5000000 INFO @ Tue, 30 Jun 2020 00:12:38: 12000000 INFO @ Tue, 30 Jun 2020 00:12:39: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:12:39: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:12:39: #1 total tags in treatment: 12013004 INFO @ Tue, 30 Jun 2020 00:12:39: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:12:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:12:39: #1 tags after filtering in treatment: 12013004 INFO @ Tue, 30 Jun 2020 00:12:39: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:12:39: #1 finished! INFO @ Tue, 30 Jun 2020 00:12:39: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:12:39: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:12:39: #2 number of paired peaks: 84 WARNING @ Tue, 30 Jun 2020 00:12:39: Too few paired peaks (84) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:12:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6386732/SRX6386732.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6386732/SRX6386732.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6386732/SRX6386732.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6386732/SRX6386732.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:12:40: 6000000 INFO @ Tue, 30 Jun 2020 00:12:45: 7000000 INFO @ Tue, 30 Jun 2020 00:12:50: 8000000 INFO @ Tue, 30 Jun 2020 00:12:55: 9000000 INFO @ Tue, 30 Jun 2020 00:12:59: 10000000 INFO @ Tue, 30 Jun 2020 00:13:04: 11000000 INFO @ Tue, 30 Jun 2020 00:13:09: 12000000 INFO @ Tue, 30 Jun 2020 00:13:09: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:13:09: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:13:09: #1 total tags in treatment: 12013004 INFO @ Tue, 30 Jun 2020 00:13:09: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:13:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:13:09: #1 tags after filtering in treatment: 12013004 INFO @ Tue, 30 Jun 2020 00:13:09: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:13:09: #1 finished! INFO @ Tue, 30 Jun 2020 00:13:09: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:13:09: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:13:10: #2 number of paired peaks: 84 WARNING @ Tue, 30 Jun 2020 00:13:10: Too few paired peaks (84) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:13:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX6386732/SRX6386732.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6386732/SRX6386732.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6386732/SRX6386732.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX6386732/SRX6386732.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。