Job ID = 3785899 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2019-11-01T05:37:15 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:37:15 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:37:15 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:37:15 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:38:05 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:38:29 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:38:29 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:39:20 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:39:20 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:39:28 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:39:49 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:39:49 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:40:29 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:40:29 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:40:29 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:42:53 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:43:10 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:43:21 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:43:21 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:43:39 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:44:12 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:45:14 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:45:15 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:45:32 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:45:55 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:45:56 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:46:45 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:46:53 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:46:54 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:47:01 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:48:25 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:48:25 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:48:30 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:48:30 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:49:25 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:49:25 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:50:35 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:50:35 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:50:46 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:51:10 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - mbedtls_ssl_handshake returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:51:10 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - ktls_handshake failed while accessing '130.14.250.27' from '172.19.7.66' 2019-11-01T05:51:10 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - Failed to create TLS stream for 'sra-download.ncbi.nlm.nih.gov' (130.14.250.27) from '172.19.7.66' 2019-11-01T05:51:10 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - mbedtls_ssl_handshake returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:51:10 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - ktls_handshake failed while accessing '130.14.250.26' from '172.19.7.66' 2019-11-01T05:51:10 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - Failed to create TLS stream for 'sra-download.ncbi.nlm.nih.gov' (130.14.250.26) from '172.19.7.66' 2019-11-01T05:51:32 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:52:33 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:53:06 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:53:13 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:54:51 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:55:22 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:55:22 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:55:43 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:56:08 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:57:44 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:57:44 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:57:47 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:58:12 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:58:57 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:59:09 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:59:32 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T05:59:54 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T06:00:35 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T06:00:39 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T06:01:49 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 40,463,033 reads read : 40,463,033 reads written : 40,463,033 2019-11-01T06:10:49 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-11-01T06:10:49 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 15,459,531 reads read : 15,459,531 reads written : 15,459,531 fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:29 55922564 reads; of these: 55922564 (100.00%) were unpaired; of these: 45898930 (82.08%) aligned 0 times 7186295 (12.85%) aligned exactly 1 time 2837339 (5.07%) aligned >1 times 17.92% overall alignment rate Time searching: 00:09:29 Overall time: 00:09:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1206306 / 10023634 = 0.1203 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Fri, 01 Nov 2019 15:25:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5849998/SRX5849998.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5849998/SRX5849998.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5849998/SRX5849998.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5849998/SRX5849998.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 01 Nov 2019 15:25:14: #1 read tag files... INFO @ Fri, 01 Nov 2019 15:25:14: #1 read treatment tags... INFO @ Fri, 01 Nov 2019 15:25:22: 1000000 INFO @ Fri, 01 Nov 2019 15:25:30: 2000000 INFO @ Fri, 01 Nov 2019 15:25:38: 3000000 INFO @ Fri, 01 Nov 2019 15:25:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5849998/SRX5849998.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5849998/SRX5849998.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5849998/SRX5849998.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5849998/SRX5849998.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 01 Nov 2019 15:25:44: #1 read tag files... INFO @ Fri, 01 Nov 2019 15:25:44: #1 read treatment tags... INFO @ Fri, 01 Nov 2019 15:25:46: 4000000 INFO @ Fri, 01 Nov 2019 15:25:53: 5000000 INFO @ Fri, 01 Nov 2019 15:25:53: 1000000 INFO @ Fri, 01 Nov 2019 15:26:01: 6000000 INFO @ Fri, 01 Nov 2019 15:26:02: 2000000 INFO @ Fri, 01 Nov 2019 15:26:08: 7000000 INFO @ Fri, 01 Nov 2019 15:26:11: 3000000 BedGraph に変換中... INFO @ Fri, 01 Nov 2019 15:26:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5849998/SRX5849998.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5849998/SRX5849998.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5849998/SRX5849998.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5849998/SRX5849998.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 01 Nov 2019 15:26:14: #1 read tag files... INFO @ Fri, 01 Nov 2019 15:26:14: #1 read treatment tags... INFO @ Fri, 01 Nov 2019 15:26:16: 8000000 INFO @ Fri, 01 Nov 2019 15:26:19: 4000000 INFO @ Fri, 01 Nov 2019 15:26:22: #1 tag size is determined as 50 bps INFO @ Fri, 01 Nov 2019 15:26:22: #1 tag size = 50 INFO @ Fri, 01 Nov 2019 15:26:22: #1 total tags in treatment: 8817328 INFO @ Fri, 01 Nov 2019 15:26:22: #1 user defined the maximum tags... INFO @ Fri, 01 Nov 2019 15:26:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 01 Nov 2019 15:26:23: #1 tags after filtering in treatment: 8817328 INFO @ Fri, 01 Nov 2019 15:26:23: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 01 Nov 2019 15:26:23: #1 finished! INFO @ Fri, 01 Nov 2019 15:26:23: #2 Build Peak Model... INFO @ Fri, 01 Nov 2019 15:26:23: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 01 Nov 2019 15:26:23: 1000000 INFO @ Fri, 01 Nov 2019 15:26:24: #2 number of paired peaks: 5266 INFO @ Fri, 01 Nov 2019 15:26:24: start model_add_line... INFO @ Fri, 01 Nov 2019 15:26:24: start X-correlation... INFO @ Fri, 01 Nov 2019 15:26:24: end of X-cor INFO @ Fri, 01 Nov 2019 15:26:24: #2 finished! INFO @ Fri, 01 Nov 2019 15:26:24: #2 predicted fragment length is 185 bps INFO @ Fri, 01 Nov 2019 15:26:24: #2 alternative fragment length(s) may be 2,185 bps INFO @ Fri, 01 Nov 2019 15:26:24: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX5849998/SRX5849998.05_model.r INFO @ Fri, 01 Nov 2019 15:26:24: #3 Call peaks... INFO @ Fri, 01 Nov 2019 15:26:24: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 01 Nov 2019 15:26:28: 5000000 INFO @ Fri, 01 Nov 2019 15:26:31: 2000000 INFO @ Fri, 01 Nov 2019 15:26:37: 6000000 INFO @ Fri, 01 Nov 2019 15:26:38: 3000000 INFO @ Fri, 01 Nov 2019 15:26:46: 7000000 INFO @ Fri, 01 Nov 2019 15:26:46: 4000000 INFO @ Fri, 01 Nov 2019 15:26:54: 5000000 INFO @ Fri, 01 Nov 2019 15:26:54: 8000000 INFO @ Fri, 01 Nov 2019 15:26:56: #3 Call peaks for each chromosome... INFO @ Fri, 01 Nov 2019 15:27:01: #1 tag size is determined as 50 bps INFO @ Fri, 01 Nov 2019 15:27:01: #1 tag size = 50 INFO @ Fri, 01 Nov 2019 15:27:01: #1 total tags in treatment: 8817328 INFO @ Fri, 01 Nov 2019 15:27:01: #1 user defined the maximum tags... INFO @ Fri, 01 Nov 2019 15:27:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 01 Nov 2019 15:27:01: #1 tags after filtering in treatment: 8817328 INFO @ Fri, 01 Nov 2019 15:27:01: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 01 Nov 2019 15:27:01: #1 finished! INFO @ Fri, 01 Nov 2019 15:27:01: #2 Build Peak Model... INFO @ Fri, 01 Nov 2019 15:27:01: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 01 Nov 2019 15:27:02: 6000000 INFO @ Fri, 01 Nov 2019 15:27:03: #2 number of paired peaks: 5266 INFO @ Fri, 01 Nov 2019 15:27:03: start model_add_line... INFO @ Fri, 01 Nov 2019 15:27:03: start X-correlation... INFO @ Fri, 01 Nov 2019 15:27:03: end of X-cor INFO @ Fri, 01 Nov 2019 15:27:03: #2 finished! INFO @ Fri, 01 Nov 2019 15:27:03: #2 predicted fragment length is 185 bps INFO @ Fri, 01 Nov 2019 15:27:03: #2 alternative fragment length(s) may be 2,185 bps INFO @ Fri, 01 Nov 2019 15:27:03: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX5849998/SRX5849998.10_model.r INFO @ Fri, 01 Nov 2019 15:27:03: #3 Call peaks... INFO @ Fri, 01 Nov 2019 15:27:03: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 01 Nov 2019 15:27:10: 7000000 INFO @ Fri, 01 Nov 2019 15:27:11: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX5849998/SRX5849998.05_peaks.xls INFO @ Fri, 01 Nov 2019 15:27:11: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX5849998/SRX5849998.05_peaks.narrowPeak INFO @ Fri, 01 Nov 2019 15:27:11: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX5849998/SRX5849998.05_summits.bed INFO @ Fri, 01 Nov 2019 15:27:11: Done! pass1 - making usageList (15 chroms): 4 millis pass2 - checking and writing primary data (7256 records, 4 fields): 18 millis CompletedMACS2peakCalling INFO @ Fri, 01 Nov 2019 15:27:18: 8000000 INFO @ Fri, 01 Nov 2019 15:27:24: #1 tag size is determined as 50 bps INFO @ Fri, 01 Nov 2019 15:27:24: #1 tag size = 50 INFO @ Fri, 01 Nov 2019 15:27:24: #1 total tags in treatment: 8817328 INFO @ Fri, 01 Nov 2019 15:27:24: #1 user defined the maximum tags... INFO @ Fri, 01 Nov 2019 15:27:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 01 Nov 2019 15:27:24: #1 tags after filtering in treatment: 8817328 INFO @ Fri, 01 Nov 2019 15:27:24: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 01 Nov 2019 15:27:24: #1 finished! INFO @ Fri, 01 Nov 2019 15:27:24: #2 Build Peak Model... INFO @ Fri, 01 Nov 2019 15:27:24: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 01 Nov 2019 15:27:25: #2 number of paired peaks: 5266 INFO @ Fri, 01 Nov 2019 15:27:25: start model_add_line... INFO @ Fri, 01 Nov 2019 15:27:26: start X-correlation... INFO @ Fri, 01 Nov 2019 15:27:26: end of X-cor INFO @ Fri, 01 Nov 2019 15:27:26: #2 finished! INFO @ Fri, 01 Nov 2019 15:27:26: #2 predicted fragment length is 185 bps INFO @ Fri, 01 Nov 2019 15:27:26: #2 alternative fragment length(s) may be 2,185 bps INFO @ Fri, 01 Nov 2019 15:27:26: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX5849998/SRX5849998.20_model.r INFO @ Fri, 01 Nov 2019 15:27:26: #3 Call peaks... INFO @ Fri, 01 Nov 2019 15:27:26: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 01 Nov 2019 15:27:34: #3 Call peaks for each chromosome... INFO @ Fri, 01 Nov 2019 15:27:48: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX5849998/SRX5849998.10_peaks.xls INFO @ Fri, 01 Nov 2019 15:27:48: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX5849998/SRX5849998.10_peaks.narrowPeak INFO @ Fri, 01 Nov 2019 15:27:48: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX5849998/SRX5849998.10_summits.bed INFO @ Fri, 01 Nov 2019 15:27:49: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (3362 records, 4 fields): 10 millis CompletedMACS2peakCalling INFO @ Fri, 01 Nov 2019 15:27:56: #3 Call peaks for each chromosome... INFO @ Fri, 01 Nov 2019 15:28:11: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX5849998/SRX5849998.20_peaks.xls INFO @ Fri, 01 Nov 2019 15:28:11: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX5849998/SRX5849998.20_peaks.narrowPeak INFO @ Fri, 01 Nov 2019 15:28:11: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX5849998/SRX5849998.20_summits.bed INFO @ Fri, 01 Nov 2019 15:28:11: Done! pass1 - making usageList (14 chroms): 1 millis pass2 - checking and writing primary data (1188 records, 4 fields): 5 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。