Job ID = 4178582 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 49,600,736 reads read : 99,201,472 reads written : 49,600,736 reads 0-length : 49,600,736 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:20:03 49600736 reads; of these: 49600736 (100.00%) were unpaired; of these: 4213115 (8.49%) aligned 0 times 32829074 (66.19%) aligned exactly 1 time 12558547 (25.32%) aligned >1 times 91.51% overall alignment rate Time searching: 00:20:03 Overall time: 00:20:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 13300193 / 45387621 = 0.2930 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Thu, 05 Dec 2019 14:01:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5827846/SRX5827846.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5827846/SRX5827846.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5827846/SRX5827846.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5827846/SRX5827846.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Dec 2019 14:01:53: #1 read tag files... INFO @ Thu, 05 Dec 2019 14:01:53: #1 read treatment tags... INFO @ Thu, 05 Dec 2019 14:02:02: 1000000 INFO @ Thu, 05 Dec 2019 14:02:10: 2000000 INFO @ Thu, 05 Dec 2019 14:02:19: 3000000 INFO @ Thu, 05 Dec 2019 14:02:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5827846/SRX5827846.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5827846/SRX5827846.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5827846/SRX5827846.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5827846/SRX5827846.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Dec 2019 14:02:23: #1 read tag files... INFO @ Thu, 05 Dec 2019 14:02:23: #1 read treatment tags... INFO @ Thu, 05 Dec 2019 14:02:28: 4000000 INFO @ Thu, 05 Dec 2019 14:02:32: 1000000 INFO @ Thu, 05 Dec 2019 14:02:37: 5000000 INFO @ Thu, 05 Dec 2019 14:02:41: 2000000 INFO @ Thu, 05 Dec 2019 14:02:46: 6000000 INFO @ Thu, 05 Dec 2019 14:02:50: 3000000 BedGraph に変換中... INFO @ Thu, 05 Dec 2019 14:02:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5827846/SRX5827846.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5827846/SRX5827846.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5827846/SRX5827846.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5827846/SRX5827846.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 05 Dec 2019 14:02:53: #1 read tag files... INFO @ Thu, 05 Dec 2019 14:02:53: #1 read treatment tags... INFO @ Thu, 05 Dec 2019 14:02:56: 7000000 INFO @ Thu, 05 Dec 2019 14:02:59: 4000000 INFO @ Thu, 05 Dec 2019 14:03:03: 1000000 INFO @ Thu, 05 Dec 2019 14:03:05: 8000000 INFO @ Thu, 05 Dec 2019 14:03:08: 5000000 INFO @ Thu, 05 Dec 2019 14:03:13: 2000000 INFO @ Thu, 05 Dec 2019 14:03:14: 9000000 INFO @ Thu, 05 Dec 2019 14:03:18: 6000000 INFO @ Thu, 05 Dec 2019 14:03:23: 3000000 INFO @ Thu, 05 Dec 2019 14:03:26: 10000000 INFO @ Thu, 05 Dec 2019 14:03:31: 7000000 INFO @ Thu, 05 Dec 2019 14:03:35: 4000000 INFO @ Thu, 05 Dec 2019 14:03:36: 11000000 INFO @ Thu, 05 Dec 2019 14:03:42: 8000000 INFO @ Thu, 05 Dec 2019 14:03:43: 5000000 INFO @ Thu, 05 Dec 2019 14:03:47: 12000000 INFO @ Thu, 05 Dec 2019 14:03:52: 6000000 INFO @ Thu, 05 Dec 2019 14:03:53: 9000000 INFO @ Thu, 05 Dec 2019 14:03:56: 13000000 INFO @ Thu, 05 Dec 2019 14:04:01: 7000000 INFO @ Thu, 05 Dec 2019 14:04:03: 10000000 INFO @ Thu, 05 Dec 2019 14:04:06: 14000000 INFO @ Thu, 05 Dec 2019 14:04:10: 8000000 INFO @ Thu, 05 Dec 2019 14:04:14: 11000000 INFO @ Thu, 05 Dec 2019 14:04:16: 15000000 INFO @ Thu, 05 Dec 2019 14:04:19: 9000000 INFO @ Thu, 05 Dec 2019 14:04:24: 12000000 INFO @ Thu, 05 Dec 2019 14:04:27: 16000000 INFO @ Thu, 05 Dec 2019 14:04:27: 10000000 INFO @ Thu, 05 Dec 2019 14:04:33: 13000000 INFO @ Thu, 05 Dec 2019 14:04:37: 11000000 INFO @ Thu, 05 Dec 2019 14:04:37: 17000000 INFO @ Thu, 05 Dec 2019 14:04:43: 14000000 INFO @ Thu, 05 Dec 2019 14:04:47: 12000000 INFO @ Thu, 05 Dec 2019 14:04:47: 18000000 INFO @ Thu, 05 Dec 2019 14:04:53: 15000000 INFO @ Thu, 05 Dec 2019 14:04:57: 13000000 INFO @ Thu, 05 Dec 2019 14:04:57: 19000000 INFO @ Thu, 05 Dec 2019 14:05:03: 16000000 INFO @ Thu, 05 Dec 2019 14:05:06: 14000000 INFO @ Thu, 05 Dec 2019 14:05:08: 20000000 INFO @ Thu, 05 Dec 2019 14:05:14: 17000000 INFO @ Thu, 05 Dec 2019 14:05:16: 15000000 INFO @ Thu, 05 Dec 2019 14:05:18: 21000000 INFO @ Thu, 05 Dec 2019 14:05:25: 18000000 INFO @ Thu, 05 Dec 2019 14:05:26: 16000000 INFO @ Thu, 05 Dec 2019 14:05:27: 22000000 INFO @ Thu, 05 Dec 2019 14:05:36: 17000000 INFO @ Thu, 05 Dec 2019 14:05:36: 19000000 INFO @ Thu, 05 Dec 2019 14:05:37: 23000000 INFO @ Thu, 05 Dec 2019 14:05:46: 24000000 INFO @ Thu, 05 Dec 2019 14:05:46: 18000000 INFO @ Thu, 05 Dec 2019 14:05:47: 20000000 INFO @ Thu, 05 Dec 2019 14:05:56: 25000000 INFO @ Thu, 05 Dec 2019 14:05:56: 19000000 INFO @ Thu, 05 Dec 2019 14:05:57: 21000000 INFO @ Thu, 05 Dec 2019 14:06:06: 26000000 INFO @ Thu, 05 Dec 2019 14:06:06: 20000000 INFO @ Thu, 05 Dec 2019 14:06:07: 22000000 INFO @ Thu, 05 Dec 2019 14:06:16: 21000000 INFO @ Thu, 05 Dec 2019 14:06:16: 27000000 INFO @ Thu, 05 Dec 2019 14:06:17: 23000000 INFO @ Thu, 05 Dec 2019 14:06:25: 22000000 INFO @ Thu, 05 Dec 2019 14:06:26: 28000000 INFO @ Thu, 05 Dec 2019 14:06:26: 24000000 INFO @ Thu, 05 Dec 2019 14:06:35: 23000000 INFO @ Thu, 05 Dec 2019 14:06:36: 29000000 INFO @ Thu, 05 Dec 2019 14:06:36: 25000000 INFO @ Thu, 05 Dec 2019 14:06:45: 24000000 INFO @ Thu, 05 Dec 2019 14:06:45: 30000000 INFO @ Thu, 05 Dec 2019 14:06:47: 26000000 INFO @ Thu, 05 Dec 2019 14:06:55: 25000000 INFO @ Thu, 05 Dec 2019 14:06:55: 31000000 INFO @ Thu, 05 Dec 2019 14:06:57: 27000000 INFO @ Thu, 05 Dec 2019 14:07:05: 32000000 INFO @ Thu, 05 Dec 2019 14:07:06: 26000000 INFO @ Thu, 05 Dec 2019 14:07:06: #1 tag size is determined as 51 bps INFO @ Thu, 05 Dec 2019 14:07:06: #1 tag size = 51 INFO @ Thu, 05 Dec 2019 14:07:06: #1 total tags in treatment: 32087428 INFO @ Thu, 05 Dec 2019 14:07:06: #1 user defined the maximum tags... INFO @ Thu, 05 Dec 2019 14:07:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Dec 2019 14:07:06: 28000000 INFO @ Thu, 05 Dec 2019 14:07:07: #1 tags after filtering in treatment: 32087428 INFO @ Thu, 05 Dec 2019 14:07:07: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Dec 2019 14:07:07: #1 finished! INFO @ Thu, 05 Dec 2019 14:07:07: #2 Build Peak Model... INFO @ Thu, 05 Dec 2019 14:07:07: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Dec 2019 14:07:09: #2 number of paired peaks: 64 WARNING @ Thu, 05 Dec 2019 14:07:09: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Dec 2019 14:07:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5827846/SRX5827846.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5827846/SRX5827846.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5827846/SRX5827846.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5827846/SRX5827846.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 05 Dec 2019 14:07:15: 29000000 INFO @ Thu, 05 Dec 2019 14:07:16: 27000000 INFO @ Thu, 05 Dec 2019 14:07:24: 30000000 INFO @ Thu, 05 Dec 2019 14:07:27: 28000000 INFO @ Thu, 05 Dec 2019 14:07:32: 31000000 INFO @ Thu, 05 Dec 2019 14:07:38: 29000000 INFO @ Thu, 05 Dec 2019 14:07:41: 32000000 INFO @ Thu, 05 Dec 2019 14:07:42: #1 tag size is determined as 51 bps INFO @ Thu, 05 Dec 2019 14:07:42: #1 tag size = 51 INFO @ Thu, 05 Dec 2019 14:07:42: #1 total tags in treatment: 32087428 INFO @ Thu, 05 Dec 2019 14:07:42: #1 user defined the maximum tags... INFO @ Thu, 05 Dec 2019 14:07:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Dec 2019 14:07:43: #1 tags after filtering in treatment: 32087428 INFO @ Thu, 05 Dec 2019 14:07:43: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Dec 2019 14:07:43: #1 finished! INFO @ Thu, 05 Dec 2019 14:07:43: #2 Build Peak Model... INFO @ Thu, 05 Dec 2019 14:07:43: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Dec 2019 14:07:45: #2 number of paired peaks: 64 WARNING @ Thu, 05 Dec 2019 14:07:45: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Dec 2019 14:07:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5827846/SRX5827846.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5827846/SRX5827846.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5827846/SRX5827846.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5827846/SRX5827846.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 05 Dec 2019 14:07:48: 30000000 INFO @ Thu, 05 Dec 2019 14:07:59: 31000000 INFO @ Thu, 05 Dec 2019 14:08:09: 32000000 INFO @ Thu, 05 Dec 2019 14:08:10: #1 tag size is determined as 51 bps INFO @ Thu, 05 Dec 2019 14:08:10: #1 tag size = 51 INFO @ Thu, 05 Dec 2019 14:08:10: #1 total tags in treatment: 32087428 INFO @ Thu, 05 Dec 2019 14:08:10: #1 user defined the maximum tags... INFO @ Thu, 05 Dec 2019 14:08:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 05 Dec 2019 14:08:11: #1 tags after filtering in treatment: 32087428 INFO @ Thu, 05 Dec 2019 14:08:11: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 05 Dec 2019 14:08:11: #1 finished! INFO @ Thu, 05 Dec 2019 14:08:11: #2 Build Peak Model... INFO @ Thu, 05 Dec 2019 14:08:11: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 05 Dec 2019 14:08:14: #2 number of paired peaks: 64 WARNING @ Thu, 05 Dec 2019 14:08:14: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 05 Dec 2019 14:08:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5827846/SRX5827846.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5827846/SRX5827846.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5827846/SRX5827846.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5827846/SRX5827846.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。