Job ID = 6528403 SRX = SRX5775930 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T15:31:42 prefetch.2.10.7: 1) Downloading 'SRR8997133'... 2020-06-29T15:31:42 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:33:52 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:33:52 prefetch.2.10.7: 1) 'SRR8997133' was downloaded successfully Read 20713487 spots for SRR8997133/SRR8997133.sra Written 20713487 spots for SRR8997133/SRR8997133.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:11 20713487 reads; of these: 20713487 (100.00%) were unpaired; of these: 531657 (2.57%) aligned 0 times 13882377 (67.02%) aligned exactly 1 time 6299453 (30.41%) aligned >1 times 97.43% overall alignment rate Time searching: 00:10:11 Overall time: 00:10:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 7509601 / 20181830 = 0.3721 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:01:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5775930/SRX5775930.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5775930/SRX5775930.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5775930/SRX5775930.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5775930/SRX5775930.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:01:01: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:01:01: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:01:07: 1000000 INFO @ Tue, 30 Jun 2020 01:01:12: 2000000 INFO @ Tue, 30 Jun 2020 01:01:18: 3000000 INFO @ Tue, 30 Jun 2020 01:01:23: 4000000 INFO @ Tue, 30 Jun 2020 01:01:29: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:01:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5775930/SRX5775930.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5775930/SRX5775930.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5775930/SRX5775930.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5775930/SRX5775930.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:01:31: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:01:31: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:01:34: 6000000 INFO @ Tue, 30 Jun 2020 01:01:37: 1000000 INFO @ Tue, 30 Jun 2020 01:01:40: 7000000 INFO @ Tue, 30 Jun 2020 01:01:43: 2000000 INFO @ Tue, 30 Jun 2020 01:01:47: 8000000 INFO @ Tue, 30 Jun 2020 01:01:50: 3000000 INFO @ Tue, 30 Jun 2020 01:01:53: 9000000 INFO @ Tue, 30 Jun 2020 01:01:56: 4000000 INFO @ Tue, 30 Jun 2020 01:01:59: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:02:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5775930/SRX5775930.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5775930/SRX5775930.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5775930/SRX5775930.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5775930/SRX5775930.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:02:01: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:02:01: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:02:02: 5000000 INFO @ Tue, 30 Jun 2020 01:02:05: 11000000 INFO @ Tue, 30 Jun 2020 01:02:08: 1000000 INFO @ Tue, 30 Jun 2020 01:02:08: 6000000 INFO @ Tue, 30 Jun 2020 01:02:12: 12000000 INFO @ Tue, 30 Jun 2020 01:02:15: 2000000 INFO @ Tue, 30 Jun 2020 01:02:15: 7000000 INFO @ Tue, 30 Jun 2020 01:02:16: #1 tag size is determined as 75 bps INFO @ Tue, 30 Jun 2020 01:02:16: #1 tag size = 75 INFO @ Tue, 30 Jun 2020 01:02:16: #1 total tags in treatment: 12672229 INFO @ Tue, 30 Jun 2020 01:02:16: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:02:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:02:16: #1 tags after filtering in treatment: 12672229 INFO @ Tue, 30 Jun 2020 01:02:16: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:02:16: #1 finished! INFO @ Tue, 30 Jun 2020 01:02:16: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:02:16: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:02:17: #2 number of paired peaks: 98 WARNING @ Tue, 30 Jun 2020 01:02:17: Too few paired peaks (98) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:02:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5775930/SRX5775930.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5775930/SRX5775930.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5775930/SRX5775930.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5775930/SRX5775930.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 01:02:21: 8000000 INFO @ Tue, 30 Jun 2020 01:02:21: 3000000 INFO @ Tue, 30 Jun 2020 01:02:27: 9000000 INFO @ Tue, 30 Jun 2020 01:02:27: 4000000 INFO @ Tue, 30 Jun 2020 01:02:33: 10000000 INFO @ Tue, 30 Jun 2020 01:02:33: 5000000 INFO @ Tue, 30 Jun 2020 01:02:39: 11000000 INFO @ Tue, 30 Jun 2020 01:02:39: 6000000 INFO @ Tue, 30 Jun 2020 01:02:45: 12000000 INFO @ Tue, 30 Jun 2020 01:02:45: 7000000 INFO @ Tue, 30 Jun 2020 01:02:49: #1 tag size is determined as 75 bps INFO @ Tue, 30 Jun 2020 01:02:49: #1 tag size = 75 INFO @ Tue, 30 Jun 2020 01:02:49: #1 total tags in treatment: 12672229 INFO @ Tue, 30 Jun 2020 01:02:49: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:02:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:02:49: #1 tags after filtering in treatment: 12672229 INFO @ Tue, 30 Jun 2020 01:02:49: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:02:49: #1 finished! INFO @ Tue, 30 Jun 2020 01:02:49: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:02:49: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:02:50: #2 number of paired peaks: 98 WARNING @ Tue, 30 Jun 2020 01:02:50: Too few paired peaks (98) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:02:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5775930/SRX5775930.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5775930/SRX5775930.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5775930/SRX5775930.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5775930/SRX5775930.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 01:02:51: 8000000 INFO @ Tue, 30 Jun 2020 01:02:56: 9000000 INFO @ Tue, 30 Jun 2020 01:03:02: 10000000 INFO @ Tue, 30 Jun 2020 01:03:07: 11000000 INFO @ Tue, 30 Jun 2020 01:03:13: 12000000 INFO @ Tue, 30 Jun 2020 01:03:17: #1 tag size is determined as 75 bps INFO @ Tue, 30 Jun 2020 01:03:17: #1 tag size = 75 INFO @ Tue, 30 Jun 2020 01:03:17: #1 total tags in treatment: 12672229 INFO @ Tue, 30 Jun 2020 01:03:17: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:03:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:03:17: #1 tags after filtering in treatment: 12672229 INFO @ Tue, 30 Jun 2020 01:03:17: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 01:03:17: #1 finished! INFO @ Tue, 30 Jun 2020 01:03:17: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:03:17: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:03:18: #2 number of paired peaks: 98 WARNING @ Tue, 30 Jun 2020 01:03:18: Too few paired peaks (98) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:03:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5775930/SRX5775930.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5775930/SRX5775930.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5775930/SRX5775930.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5775930/SRX5775930.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。