Job ID = 6528401 SRX = SRX5775908 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:55:43 prefetch.2.10.7: 1) Downloading 'SRR8997111'... 2020-06-29T14:55:43 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:58:20 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:58:20 prefetch.2.10.7: 1) 'SRR8997111' was downloaded successfully Read 27099927 spots for SRR8997111/SRR8997111.sra Written 27099927 spots for SRR8997111/SRR8997111.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:50 27099927 reads; of these: 27099927 (100.00%) were unpaired; of these: 596083 (2.20%) aligned 0 times 18692357 (68.98%) aligned exactly 1 time 7811487 (28.82%) aligned >1 times 97.80% overall alignment rate Time searching: 00:11:50 Overall time: 00:11:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 8542531 / 26503844 = 0.3223 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:26:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5775908/SRX5775908.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5775908/SRX5775908.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5775908/SRX5775908.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5775908/SRX5775908.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:26:47: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:26:47: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:26:52: 1000000 INFO @ Tue, 30 Jun 2020 00:26:58: 2000000 INFO @ Tue, 30 Jun 2020 00:27:04: 3000000 INFO @ Tue, 30 Jun 2020 00:27:10: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:27:16: 5000000 INFO @ Tue, 30 Jun 2020 00:27:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5775908/SRX5775908.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5775908/SRX5775908.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5775908/SRX5775908.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5775908/SRX5775908.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:27:17: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:27:17: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:27:22: 6000000 INFO @ Tue, 30 Jun 2020 00:27:25: 1000000 INFO @ Tue, 30 Jun 2020 00:27:29: 7000000 INFO @ Tue, 30 Jun 2020 00:27:33: 2000000 INFO @ Tue, 30 Jun 2020 00:27:36: 8000000 INFO @ Tue, 30 Jun 2020 00:27:41: 3000000 INFO @ Tue, 30 Jun 2020 00:27:43: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:27:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5775908/SRX5775908.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5775908/SRX5775908.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5775908/SRX5775908.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5775908/SRX5775908.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:27:47: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:27:47: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:27:49: 4000000 INFO @ Tue, 30 Jun 2020 00:27:50: 10000000 INFO @ Tue, 30 Jun 2020 00:27:54: 1000000 INFO @ Tue, 30 Jun 2020 00:27:57: 5000000 INFO @ Tue, 30 Jun 2020 00:27:58: 11000000 INFO @ Tue, 30 Jun 2020 00:28:01: 2000000 INFO @ Tue, 30 Jun 2020 00:28:05: 12000000 INFO @ Tue, 30 Jun 2020 00:28:05: 6000000 INFO @ Tue, 30 Jun 2020 00:28:08: 3000000 INFO @ Tue, 30 Jun 2020 00:28:12: 13000000 INFO @ Tue, 30 Jun 2020 00:28:13: 7000000 INFO @ Tue, 30 Jun 2020 00:28:15: 4000000 INFO @ Tue, 30 Jun 2020 00:28:19: 14000000 INFO @ Tue, 30 Jun 2020 00:28:21: 8000000 INFO @ Tue, 30 Jun 2020 00:28:22: 5000000 INFO @ Tue, 30 Jun 2020 00:28:26: 15000000 INFO @ Tue, 30 Jun 2020 00:28:29: 6000000 INFO @ Tue, 30 Jun 2020 00:28:29: 9000000 INFO @ Tue, 30 Jun 2020 00:28:33: 16000000 INFO @ Tue, 30 Jun 2020 00:28:37: 7000000 INFO @ Tue, 30 Jun 2020 00:28:37: 10000000 INFO @ Tue, 30 Jun 2020 00:28:40: 17000000 INFO @ Tue, 30 Jun 2020 00:28:44: 8000000 INFO @ Tue, 30 Jun 2020 00:28:45: 11000000 INFO @ Tue, 30 Jun 2020 00:28:47: #1 tag size is determined as 75 bps INFO @ Tue, 30 Jun 2020 00:28:47: #1 tag size = 75 INFO @ Tue, 30 Jun 2020 00:28:47: #1 total tags in treatment: 17961313 INFO @ Tue, 30 Jun 2020 00:28:47: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:28:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:28:48: #1 tags after filtering in treatment: 17961313 INFO @ Tue, 30 Jun 2020 00:28:48: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:28:48: #1 finished! INFO @ Tue, 30 Jun 2020 00:28:48: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:28:48: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:28:49: #2 number of paired peaks: 70 WARNING @ Tue, 30 Jun 2020 00:28:49: Too few paired peaks (70) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:28:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5775908/SRX5775908.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5775908/SRX5775908.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5775908/SRX5775908.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5775908/SRX5775908.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:28:51: 9000000 INFO @ Tue, 30 Jun 2020 00:28:53: 12000000 INFO @ Tue, 30 Jun 2020 00:28:57: 10000000 INFO @ Tue, 30 Jun 2020 00:29:01: 13000000 INFO @ Tue, 30 Jun 2020 00:29:04: 11000000 INFO @ Tue, 30 Jun 2020 00:29:10: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:29:11: 12000000 INFO @ Tue, 30 Jun 2020 00:29:18: 15000000 INFO @ Tue, 30 Jun 2020 00:29:18: 13000000 INFO @ Tue, 30 Jun 2020 00:29:25: 14000000 INFO @ Tue, 30 Jun 2020 00:29:26: 16000000 INFO @ Tue, 30 Jun 2020 00:29:32: 15000000 INFO @ Tue, 30 Jun 2020 00:29:34: 17000000 INFO @ Tue, 30 Jun 2020 00:29:39: 16000000 INFO @ Tue, 30 Jun 2020 00:29:42: #1 tag size is determined as 75 bps INFO @ Tue, 30 Jun 2020 00:29:42: #1 tag size = 75 INFO @ Tue, 30 Jun 2020 00:29:42: #1 total tags in treatment: 17961313 INFO @ Tue, 30 Jun 2020 00:29:42: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:29:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:29:42: #1 tags after filtering in treatment: 17961313 INFO @ Tue, 30 Jun 2020 00:29:42: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:29:42: #1 finished! INFO @ Tue, 30 Jun 2020 00:29:42: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:29:42: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:29:43: #2 number of paired peaks: 70 WARNING @ Tue, 30 Jun 2020 00:29:43: Too few paired peaks (70) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:29:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5775908/SRX5775908.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5775908/SRX5775908.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5775908/SRX5775908.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5775908/SRX5775908.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:29:46: 17000000 BigWig に変換しました。 INFO @ Tue, 30 Jun 2020 00:29:52: #1 tag size is determined as 75 bps INFO @ Tue, 30 Jun 2020 00:29:52: #1 tag size = 75 INFO @ Tue, 30 Jun 2020 00:29:52: #1 total tags in treatment: 17961313 INFO @ Tue, 30 Jun 2020 00:29:52: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:29:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:29:53: #1 tags after filtering in treatment: 17961313 INFO @ Tue, 30 Jun 2020 00:29:53: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:29:53: #1 finished! INFO @ Tue, 30 Jun 2020 00:29:53: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:29:53: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:29:54: #2 number of paired peaks: 70 WARNING @ Tue, 30 Jun 2020 00:29:54: Too few paired peaks (70) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:29:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5775908/SRX5775908.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5775908/SRX5775908.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5775908/SRX5775908.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5775908/SRX5775908.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling