Job ID = 6626501 SRX = SRX5736479 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 21238161 spots for SRR8956890/SRR8956890.sra Written 21238161 spots for SRR8956890/SRR8956890.sra fastq に変換しました。 bowtie でマッピング中... Your job 6626765 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:59:08 21238161 reads; of these: 21238161 (100.00%) were paired; of these: 2858270 (13.46%) aligned concordantly 0 times 11774373 (55.44%) aligned concordantly exactly 1 time 6605518 (31.10%) aligned concordantly >1 times ---- 2858270 pairs aligned concordantly 0 times; of these: 510470 (17.86%) aligned discordantly 1 time ---- 2347800 pairs aligned 0 times concordantly or discordantly; of these: 4695600 mates make up the pairs; of these: 3221338 (68.60%) aligned 0 times 656233 (13.98%) aligned exactly 1 time 818029 (17.42%) aligned >1 times 92.42% overall alignment rate Time searching: 00:59:09 Overall time: 00:59:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 1817703 / 18760097 = 0.0969 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:20:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5736479/SRX5736479.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5736479/SRX5736479.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5736479/SRX5736479.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5736479/SRX5736479.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:20:17: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:20:17: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:20:24: 1000000 INFO @ Tue, 14 Jul 2020 08:20:31: 2000000 INFO @ Tue, 14 Jul 2020 08:20:37: 3000000 INFO @ Tue, 14 Jul 2020 08:20:43: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:20:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5736479/SRX5736479.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5736479/SRX5736479.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5736479/SRX5736479.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5736479/SRX5736479.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:20:47: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:20:47: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:20:49: 5000000 INFO @ Tue, 14 Jul 2020 08:20:55: 1000000 INFO @ Tue, 14 Jul 2020 08:20:55: 6000000 INFO @ Tue, 14 Jul 2020 08:21:02: 7000000 INFO @ Tue, 14 Jul 2020 08:21:03: 2000000 INFO @ Tue, 14 Jul 2020 08:21:09: 8000000 INFO @ Tue, 14 Jul 2020 08:21:11: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 08:21:16: 9000000 INFO @ Tue, 14 Jul 2020 08:21:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX5736479/SRX5736479.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX5736479/SRX5736479.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX5736479/SRX5736479.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX5736479/SRX5736479.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 08:21:17: #1 read tag files... INFO @ Tue, 14 Jul 2020 08:21:17: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 08:21:19: 4000000 INFO @ Tue, 14 Jul 2020 08:21:22: 10000000 INFO @ Tue, 14 Jul 2020 08:21:25: 1000000 INFO @ Tue, 14 Jul 2020 08:21:27: 5000000 INFO @ Tue, 14 Jul 2020 08:21:29: 11000000 INFO @ Tue, 14 Jul 2020 08:21:32: 2000000 INFO @ Tue, 14 Jul 2020 08:21:35: 6000000 INFO @ Tue, 14 Jul 2020 08:21:37: 12000000 INFO @ Tue, 14 Jul 2020 08:21:39: 3000000 INFO @ Tue, 14 Jul 2020 08:21:43: 7000000 INFO @ Tue, 14 Jul 2020 08:21:44: 13000000 INFO @ Tue, 14 Jul 2020 08:21:47: 4000000 INFO @ Tue, 14 Jul 2020 08:21:50: 14000000 INFO @ Tue, 14 Jul 2020 08:21:51: 8000000 INFO @ Tue, 14 Jul 2020 08:21:56: 5000000 INFO @ Tue, 14 Jul 2020 08:21:57: 15000000 INFO @ Tue, 14 Jul 2020 08:21:59: 9000000 INFO @ Tue, 14 Jul 2020 08:22:04: 16000000 INFO @ Tue, 14 Jul 2020 08:22:04: 6000000 INFO @ Tue, 14 Jul 2020 08:22:07: 10000000 INFO @ Tue, 14 Jul 2020 08:22:11: 17000000 INFO @ Tue, 14 Jul 2020 08:22:13: 7000000 INFO @ Tue, 14 Jul 2020 08:22:15: 11000000 INFO @ Tue, 14 Jul 2020 08:22:18: 18000000 INFO @ Tue, 14 Jul 2020 08:22:22: 8000000 INFO @ Tue, 14 Jul 2020 08:22:23: 12000000 INFO @ Tue, 14 Jul 2020 08:22:24: 19000000 INFO @ Tue, 14 Jul 2020 08:22:31: 9000000 INFO @ Tue, 14 Jul 2020 08:22:31: 13000000 INFO @ Tue, 14 Jul 2020 08:22:31: 20000000 INFO @ Tue, 14 Jul 2020 08:22:38: 21000000 INFO @ Tue, 14 Jul 2020 08:22:39: 14000000 INFO @ Tue, 14 Jul 2020 08:22:39: 10000000 INFO @ Tue, 14 Jul 2020 08:22:45: 22000000 INFO @ Tue, 14 Jul 2020 08:22:47: 15000000 INFO @ Tue, 14 Jul 2020 08:22:48: 11000000 INFO @ Tue, 14 Jul 2020 08:22:51: 23000000 INFO @ Tue, 14 Jul 2020 08:22:56: 16000000 INFO @ Tue, 14 Jul 2020 08:22:57: 12000000 INFO @ Tue, 14 Jul 2020 08:22:58: 24000000 INFO @ Tue, 14 Jul 2020 08:23:05: 25000000 INFO @ Tue, 14 Jul 2020 08:23:06: 13000000 INFO @ Tue, 14 Jul 2020 08:23:06: 17000000 INFO @ Tue, 14 Jul 2020 08:23:12: 26000000 INFO @ Tue, 14 Jul 2020 08:23:15: 14000000 INFO @ Tue, 14 Jul 2020 08:23:17: 18000000 INFO @ Tue, 14 Jul 2020 08:23:18: 27000000 INFO @ Tue, 14 Jul 2020 08:23:24: 15000000 INFO @ Tue, 14 Jul 2020 08:23:25: 28000000 INFO @ Tue, 14 Jul 2020 08:23:28: 19000000 INFO @ Tue, 14 Jul 2020 08:23:32: 29000000 INFO @ Tue, 14 Jul 2020 08:23:33: 16000000 INFO @ Tue, 14 Jul 2020 08:23:38: 20000000 INFO @ Tue, 14 Jul 2020 08:23:39: 30000000 INFO @ Tue, 14 Jul 2020 08:23:42: 17000000 INFO @ Tue, 14 Jul 2020 08:23:46: 31000000 INFO @ Tue, 14 Jul 2020 08:23:48: 21000000 INFO @ Tue, 14 Jul 2020 08:23:51: 18000000 INFO @ Tue, 14 Jul 2020 08:23:52: 32000000 INFO @ Tue, 14 Jul 2020 08:23:58: 22000000 INFO @ Tue, 14 Jul 2020 08:23:59: 33000000 INFO @ Tue, 14 Jul 2020 08:24:00: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 08:24:06: 34000000 INFO @ Tue, 14 Jul 2020 08:24:08: 23000000 INFO @ Tue, 14 Jul 2020 08:24:09: 20000000 INFO @ Tue, 14 Jul 2020 08:24:13: 35000000 INFO @ Tue, 14 Jul 2020 08:24:17: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 08:24:17: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 08:24:17: #1 total tags in treatment: 16584246 INFO @ Tue, 14 Jul 2020 08:24:17: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:24:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:24:17: #1 tags after filtering in treatment: 14518913 INFO @ Tue, 14 Jul 2020 08:24:17: #1 Redundant rate of treatment: 0.12 INFO @ Tue, 14 Jul 2020 08:24:17: #1 finished! INFO @ Tue, 14 Jul 2020 08:24:17: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:24:17: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:24:17: 21000000 INFO @ Tue, 14 Jul 2020 08:24:18: #2 number of paired peaks: 58 WARNING @ Tue, 14 Jul 2020 08:24:18: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:24:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5736479/SRX5736479.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736479/SRX5736479.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736479/SRX5736479.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736479/SRX5736479.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:24:19: 24000000 INFO @ Tue, 14 Jul 2020 08:24:24: 22000000 INFO @ Tue, 14 Jul 2020 08:24:29: 25000000 INFO @ Tue, 14 Jul 2020 08:24:31: 23000000 INFO @ Tue, 14 Jul 2020 08:24:37: 24000000 INFO @ Tue, 14 Jul 2020 08:24:39: 26000000 INFO @ Tue, 14 Jul 2020 08:24:44: 25000000 INFO @ Tue, 14 Jul 2020 08:24:49: 27000000 INFO @ Tue, 14 Jul 2020 08:24:50: 26000000 INFO @ Tue, 14 Jul 2020 08:24:57: 27000000 INFO @ Tue, 14 Jul 2020 08:24:59: 28000000 INFO @ Tue, 14 Jul 2020 08:25:03: 28000000 INFO @ Tue, 14 Jul 2020 08:25:09: 29000000 INFO @ Tue, 14 Jul 2020 08:25:10: 29000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 08:25:17: 30000000 INFO @ Tue, 14 Jul 2020 08:25:20: 30000000 INFO @ Tue, 14 Jul 2020 08:25:23: 31000000 INFO @ Tue, 14 Jul 2020 08:25:30: 32000000 INFO @ Tue, 14 Jul 2020 08:25:30: 31000000 INFO @ Tue, 14 Jul 2020 08:25:36: 33000000 INFO @ Tue, 14 Jul 2020 08:25:41: 32000000 INFO @ Tue, 14 Jul 2020 08:25:43: 34000000 INFO @ Tue, 14 Jul 2020 08:25:50: 35000000 INFO @ Tue, 14 Jul 2020 08:25:51: 33000000 INFO @ Tue, 14 Jul 2020 08:25:54: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 08:25:54: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 08:25:54: #1 total tags in treatment: 16584246 INFO @ Tue, 14 Jul 2020 08:25:54: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:25:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:25:54: #1 tags after filtering in treatment: 14518913 INFO @ Tue, 14 Jul 2020 08:25:54: #1 Redundant rate of treatment: 0.12 INFO @ Tue, 14 Jul 2020 08:25:54: #1 finished! INFO @ Tue, 14 Jul 2020 08:25:54: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:25:54: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:25:55: #2 number of paired peaks: 58 WARNING @ Tue, 14 Jul 2020 08:25:55: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:25:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5736479/SRX5736479.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736479/SRX5736479.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736479/SRX5736479.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736479/SRX5736479.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 08:26:02: 34000000 INFO @ Tue, 14 Jul 2020 08:26:12: 35000000 INFO @ Tue, 14 Jul 2020 08:26:19: #1 tag size is determined as 75 bps INFO @ Tue, 14 Jul 2020 08:26:19: #1 tag size = 75 INFO @ Tue, 14 Jul 2020 08:26:19: #1 total tags in treatment: 16584246 INFO @ Tue, 14 Jul 2020 08:26:19: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 08:26:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 08:26:19: #1 tags after filtering in treatment: 14518913 INFO @ Tue, 14 Jul 2020 08:26:19: #1 Redundant rate of treatment: 0.12 INFO @ Tue, 14 Jul 2020 08:26:19: #1 finished! INFO @ Tue, 14 Jul 2020 08:26:19: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 08:26:19: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 08:26:20: #2 number of paired peaks: 58 WARNING @ Tue, 14 Jul 2020 08:26:20: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 08:26:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX5736479/SRX5736479.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736479/SRX5736479.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736479/SRX5736479.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX5736479/SRX5736479.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling